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Gene information
General information
TC012841 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15730830..15733131
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012841
    iBeetle screen iB_05335

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality),
    Pupal morphology
    beetle stock: bad (increased lethality),
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012841 RA
      MSLPLVFLY LFFPSSSVS INIKNNVCT KVASYHLAP 
      ISPDCRYNP DVLSDVQAM SQRHGYSFE KLPVTTDDG 
      YILNIFKIS SKNSVGDKL PVFVQHGIA ENSGAWADK 
      GNRSLAYRL VEEGHDVYL GNLRGSIFS NKHVKYSVN 
      DPRYWNFNL DIMAANDLR SMLNFVAKS TGSKILYIG 
      HSMGTTLSF MYSSEFSKE ASQILQGII ALAPVGFLN 
      GVPIIELAR PIGIPLLDV LSVLHIRGL LYQEKIIHK 
      LINVLCKNA VPEICYGFF SLATGPTKQ FLPEDMLTF 
      LSYWPSGLS IYQLKHYLQ IGASKKFQK YDYGRIGNL 
      KHYGSFKPP SYKLKDIKV PISLMYGEN DILFRQKNV 
      DRLFHEIGS HSKSKYAIS AGRQGYSHI DFVYAKNLE 
      DDLYQLMFD VLSSQMGSA K
    CDS sequence
    • >TC012841 RA
      atgtcgcta ccactggtg tttctctat ttatttttt 
      ccctcgtca tcagtttca atcaatata aaaaataat 
      gtttgcact aaagtggcc tcgtatcac cttgcgcca 
      atcagtcca gactgtaga tacaatcct gatgttttg 
      tccgatgtt caagcaatg tcacaacga catggctac 
      agttttgaa aaattgcca gtcacaact gacgatggc 
      tacatatta aatattttc aaaatttcg tcaaaaaat 
      tcagttggg gataaatta ccagtgttt gtgcaacat 
      ggaattgcc gaaaattcg ggcgcttgg gctgataaa 
      ggcaaccga tctttggct tataggctt gtggaggaa 
      ggacatgat gtgtattta ggaaatctg agaggttca 
      atcttttca aacaaacat gtcaaatat tcagttaac 
      gatcccaga tattggaat tttaatttg gacataatg 
      gcagcaaac gacctaaga tcaatgtta aattttgtg 
      gccaaaagc accggctca aaaatcctg tacattggc 
      cactctatg ggaacaacc ttatcgttc atgtattcg 
      tccgagttt agcaaagaa gcgagtcag atccttcag 
      ggaattatc gcactagca ccagttgga tttttgaac 
      ggagtccca attatagaa ctggctaga ccaatcgga 
      atcccactt cttgacgtg ttatccgtg ttacacatt 
      cggggactt ttgtaccag gaaaaaatt atccacaaa 
      ttgataaat gtcctttgc aagaacgca gttcctgag 
      atttgttac gggtttttc agccttgcc actggacca 
      actaaacaa ttcttgcct gaagacatg ttgacgttt 
      ttgagctac tggccgagt ggtttatcc atttaccaa 
      ctgaaacac tacttacaa atcggtgcg agtaaaaaa 
      tttcagaaa tacgattac ggccgtatt ggtaatttg 
      aaacactac ggttctttc aaaccccct agttacaaa 
      ctcaaggat attaaagtc cccatctcg ctaatgtac 
      ggcgaaaat gacattctt ttcaggcaa aaaaacgtg 
      gaccgatta tttcacgaa attggctct cacagtaaa 
      agcaagtat gcaatttcg gccggtcgg cagggttat 
      tctcacatt gactttgtt tatgccaaa aacttggag 
      gatgatttg taccagttg atgtttgat gttttaagc 
      tcgcaaatg ggaagcgcc aaataa
    mRNA sequence
    • >TC012841 RA
      ggtttaaag taaaaaaaa gtgtgtcac cacagtgtg 
      aaaaaatct tgttgccaa atgtcgcta ccactggtg 
      tttctctat ttatttttt ccctcgtca tcagtttca 
      atcaatata aaaaataat gtttgcact aaagtggcc 
      tcgtatcac cttgcgcca atcagtcca gactgtaga 
      tacaatcct gatgttttg tccgatgtt caagcaatg 
      tcacaacga catggctac agttttgaa aaattgcca 
      gtcacaact gacgatggc tacatatta aatattttc 
      aaaatttcg tcaaaaaat tcagttggg gataaatta 
      ccagtgttt gtgcaacat ggaattgcc gaaaattcg 
      ggcgcttgg gctgataaa ggcaaccga tctttggct 
      tataggctt gtggaggaa ggacatgat gtgtattta 
      ggaaatctg agaggttca atcttttca aacaaacat 
      gtcaaatat tcagttaac gatcccaga tattggaat 
      tttaatttg gacataatg gcagcaaac gacctaaga 
      tcaatgtta aattttgtg gccaaaagc accggctca 
      aaaatcctg tacattggc cactctatg ggaacaacc 
      ttatcgttc atgtattcg tccgagttt agcaaagaa 
      gcgagtcag atccttcag ggaattatc gcactagca 
      ccagttgga tttttgaac ggagtccca attatagaa 
      ctggctaga ccaatcgga atcccactt cttgacgtg 
      ttatccgtg ttacacatt cggggactt ttgtaccag 
      gaaaaaatt atccacaaa ttgataaat gtcctttgc 
      aagaacgca gttcctgag atttgttac gggtttttc 
      agccttgcc actggacca actaaacaa ttcttgcct 
      gaagacatg ttgacgttt ttgagctac tggccgagt 
      ggtttatcc atttaccaa ctgaaacac tacttacaa 
      atcggtgcg agtaaaaaa tttcagaaa tacgattac 
      ggccgtatt ggtaatttg aaacactac ggttctttc 
      aaaccccct agttacaaa ctcaaggat attaaagtc 
      cccatctcg ctaatgtac ggcgaaaat gacattctt 
      ttcaggcaa aaaaacgtg gaccgatta tttcacgaa 
      attggctct cacagtaaa agcaagtat gcaatttcg 
      gccggtcgg cagggttat tctcacatt gactttgtt 
      tatgccaaa aacttggag gatgatttg taccagttg 
      atgtttgat gttttaagc tcgcaaatg ggaagcgcc 
      aaataattt tttaagcaa caacgactt gtctgttat 
      taagtgcaa taaagtaac cgcattgat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY