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Gene information
General information
TC012840 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15739238..15740361
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012840
    iBeetle screen iB_05334

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality),
    Pupal morphology
    beetle stock: bad (increased lethality),
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012840 RA
      MSVLVNETY TEDEIFGDL ATHEESSLP STGSILQML 
      WNIIAEGTK LVICVLSAV SASFLIYII LKFEKVRKK 
      CNIFLLHYA ISSLIDVML VPLVMLMSQ FAFSSTVGW 
      EFFCFIYSI NSSTNILVF VFGSMIGFY WFIQNFKNS 
      WIKNQKKFE LFATIFIYI VCSIKLAIV GGECFVEES 
      LHTSEILRG IFLILLLII TGINIVVKR KSLTDTQIK 
      SKYELAVSS YIIYSFLPL FVIGFSFYF TWSHDTIDF 
      IFYFYTLAE VLAFSGTPI ITYILYQKS KHFRVAFNS 
      IFKRSVKHY DEEELNEES EEEQPAAGS NNNSGFI
    CDS sequence
    • >TC012840 RA
      atgtccgtt ttagtaaac gaaacctac accgaagat 
      gagattttc ggcgatctt gcgacccac gaggagtca 
      tcattaccc tccacagga tcaatcctt caaatgctc 
      tggaacatc atcgccgaa ggaacgaaa cttgtgatt 
      tgtgttcta tcagctgta tcagccagt tttctcatt 
      tacatcatc ctcaagttc gagaaagtg aggaagaaa 
      tgcaatatt ttcctccta cattacgcc atatcgtcg 
      ctgattgac gtcatgttg gtaccacta gtcatgttg 
      atgtctcaa tttgcgttt tcaagtaca gtgggctgg 
      gaatttttc tgctttatc tactcgatt aacagctcg 
      acgaatatt ttggttttc gtgttcggc tctatgatt 
      ggcttttac tggttcatc cagaatttt aaaaactcg 
      tggatcaaa aatcagaaa aagtttgag ctttttgca 
      acgattttc atctacata gtgtgttcc attaaattg 
      gcgatagtt ggaggcgaa tgttttgtg gaagagtcg 
      ctccatacg agcgaaatt cttcgagga attttcctc 
      atcttgttg ttaataata acagggata aatattgtg 
      gtcaagcga aaatcgctc accgacact caaattaag 
      tcaaaatac gaattggca gtttcctcc tatatcatc 
      tatagtttc cttccatta tttgtgatt gggtttagt 
      ttctacttc acgtggtca catgacacg attgatttc 
      attttttat ttttatacg cttgctgaa gttctggca 
      tttagtgga acacccatt attacgtac attctgtac 
      cagaagagc aaacatttc cgggtcgct tttaacagt 
      attttcaaa cgttcggtc aaacattac gacgaggaa 
      gaactgaat gaagaaagt gaagaggaa caaccagcg 
      gctggaagc aataataat tcggggttt atttaa
    mRNA sequence
    • >TC012840 RA
      ttgtcaata acccaatac aatgtccgt tttagtaaa 
      cgaaaccta caccgaaga tgagatttt cggcgatct 
      tgcgaccca cgaggagtc atcattacc ctccacagg 
      atcaatcct tcaaatgct ctggaacat catcgccga 
      aggaacgaa acttgtgat ttgtgttct atcagctgt 
      atcagccag ttttctcat ttacatcat cctcaagtt 
      cgagaaagt gaggaagaa atgcaatat tttcctcct 
      acattacgc catatcgtc gctgattga cgtcatgtt 
      ggtaccact agtcatgtt gatgtctca atttgcgtt 
      ttcaagtac agtgggctg ggaattttt ctgctttat 
      ctactcgat taacagctc gacgaatat tttggtttt 
      cgtgttcgg ctctatgat tggctttta ctggttcat 
      ccagaattt taaaaactc gtggatcaa aaatcagaa 
      aaagtttga gctttttgc aacgatttt catctacat 
      agtgtgttc cattaaatt ggcgatagt tggaggcga 
      atgttttgt ggaagagtc gctccatac gagcgaaat 
      tcttcgagg aattttcct catcttgtt gttaataat 
      aacagggat aaatattgt ggtcaagcg aaaatcgct 
      caccgacac tcaaattaa gtcaaaata cgaattggc 
      agtttcctc ctatatcat ctatagttt ccttccatt 
      atttgtgat tgggtttag tttctactt cacgtggtc 
      acatgacac gattgattt catttttta tttttatac 
      gcttgctga agttctggc atttagtgg aacacccat 
      tattacgta cattctgta ccagaagag caaacattt 
      ccgggtcgc ttttaacag tattttcaa acgttcggt 
      caaacatta cgacgagga agaactgaa tgaagaaag 
      tgaagagga acaaccagc ggctggaag caataataa 
      ttcggggtt tatttaaaa tgtatataa ataaatatg 
      ttatttgtt attaatacg acaagaatt tggaataat 
      tgtctctat aataaaagt tgaaggtta at
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY