General information | |||
TC012834 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 15812123..15817281 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC012834 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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Quotation | ||
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Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_05331 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 42.86% (includes death as larva, prepupa, pupa, adult)
wings potentially slightlynot closed - Number of animals showing the phenotype: 3
wings surface rough - Number of animals showing the phenotype: 5
abdominal odoriferous glands partially content not present - Number of animals showing the phenotype: 3 (41 dpi)
abdominal odoriferous glands partially colour darker - Number of animals showing the phenotype: 1 (41 dpi)
- >TC012834 RA
MFLFLFKSK YRRKCYVWQ ALCCAVVVL NVCSLVKAK CADCSGNGV CKNETCVCY DGWQGPQCQ FCGGKVRLG TSTGTIHDG LGNYSLSVK CSWLIDAPN TSITLHLEE FATECGWDH LYIFDGDSV DSPLLAVFS GLMYKGDYN IRRIPEIVA YSGSALVHF FSDDAFNMS GFNMTYRLN ACPSKISGV DCSGNGICI DQVCTCNGG WTGVACHLQ KCPDNCGQH DGRGRCEPE FGCRCESDY KGDDCSQYA PSGYWETVN VESFVPPGS ASHGAAVWR DSMYVIAGE SYSRRFMLY VYDFNGNVW ETPYISESP SQRYGHSTV IYGDKIFLY GGVMGNRGP TSEVWAFDI NAKTWENIT VKAVACNSS VLMCGPLKS AGHTATVIE NSTNRKADR MIVLFGHSP HLGYLNTVQ EYYFGTREW HIVATKGYP VKGGYGHTA TWDPLTGKI YVYGGVVSE NESAQVLSK SLYSYDPNT RVWVLLKDA PSARFLHTS TFISGGLML VFGGNTHND TLHSFGAKC YSSEFLAYD VTCDTWQVL QVPKDIHAD LARFGHSAV TFEGSLYIY GGFDGQMLS DMLKYTPGS CTNFITSNE CLSKKPGVK CVWDMRNNF CTAIQNIPV KNLTVQLDG LIIRCPELS RTMITQSLI QNENRCEKF EDCLSCVQT TSECVWCGN SCMHNKKCR NSSEANIAK LEQCPLDPS PICKQLHTC SACTSQPFC RWRFEHAKC VTHPLPSNV TAAAAEPIQ CQKVCSEYN SCLNCTQEE CIWCQNEGR CVDKNAYTA SFPYGQCRE WTTVGSKCR TRGANENSQ CSFYSTCAK CRDDPACGW CDDGSRTGL GKCMPGGYA GPTLHTHSL PSSTCPPDR WHFTTCPLC QCNGHASCI ANSSVCLPC QNLTMGTQC EHCVKGYWG NPVNGGKCQ PCECNGQAT HCHPESGKC YCTTKGLIG DHCEKCDAT NHYNSDPIN KDSCYYDLT IDYQFTFNL SKKEDRHFT QINFRNSPI KADIDADFS ITCSVLAKM NITIRRGGS KEEKPIYED FNCTTFRTK FPKSDYNFG VEDNITLTT FYVYVYDFQ PPLWIQISF SQYPKLNLQ QFFITFSSC FLLLLLFAA ILWKIKQRY DMYRRRQRL FVEMEQMAS RPFSQVLVE LEVKEKLDF DNISVIDLK RKKRDAPSP IALEPCYGN KAAVLSLLV RLPTGCEPY TVKGQSAGL AIASALVTL GNPRKMSID QVKTETKSG KSRKSQSQH PDSCL
- >TC012834 RA
atgtttttg ttcttgttc aagtcgaaa taccgtcga aaatgttac gtgtggcag gctttgtgt tgtgctgtt gtggtttta aatgtttgc tctcttgtt aaggccaaa tgtgcggat tgtagcggc aatggggtg tgtaaaaat gagacttgt gtgtgttac gacggctgg caggggccc cagtgccag ttttgcggc ggaaaagtg cgtttgggt acctccacc ggtaccatt cacgacggc ttgggcaac tactctctc agcgtgaaa tgctcctgg ttgatcgac gcccccaac acctccatc accctccac ctggaggag ttcgccacg gagtgcggt tgggaccac ctgtacatc ttcgacggc gacagcgtg gactcgccc cttctggcc gtcttcagc ggtctcatg tacaaaggc gactacaac atccgccgc atcccagaa atcgtcgct tattcgggc tccgccctc gtccatttc ttcagcgac gatgctttc aacatgtct ggcttcaac atgacctac cgcctcaac gcctgtccc tcgaaaata tcgggggtg gactgcagc ggcaacggc atttgcatt gatcaagtg tgcacatgc aacgggggc tggacgggg gtggcatgc caccttcaa aaatgcccc gataattgc ggacaacac gacggcaga ggccgctgt gagcccgaa ttcgggtgc cggtgtgaa agtgactac aagggggac gattgcagt caatacgcc cccagtggc tattgggaa acggtcaat gttgaaagt tttgtgcct ccgggatcg gcctcacat ggggctgcc gtgtggagg gactccatg tacgtcatc gccggggag agctacagt cgcagattt atgctctat gtatacgat tttaacggg aacgtctgg gagacacca tacatttcc gaaagtccc tcccaacgc tacggtcac tcgaccgtc atctatggc gataaaatc ttcctctat ggtggtgtg atgggtaat cgggggccc acgtccgaa gtgtgggct tttgatatt aatgccaaa acttgggag aatataact gtgaaagca gtggcgtgc aattcgagt gtgttgatg tgtgggccg ttgaaatcg gccggacac acagccact gtgattgag aattcgacc aatcggaaa gccgatcgt atgattgtt ttattcggt cattcgcca catcttggc tacttgaat acggtccag gagtattat tttggtaca cgggagtgg cacatagtt gcaactaag ggatatccg gtcaagggt ggctatgga catacagcc acctgggat cccttgact gggaaaatt tatgtttat ggaggagtg gtttcggaa aatgagtcg gctcaagtt ttgagtaaa agtctttat tcgtatgat cccaatacg cgagtttgg gtgttgttg aaggacgcg ccttcggct cgtttcctc cacacttcg acttttatt tcggggggt ttaatgcta gtttttggg ggaaatacc cacaacgat acgttgcac agtttcgga gcaaagtgt tacagttcg gagtttttg gcttatgac gttacgtgt gatacttgg caggtgtta caagtgcca aaggatatt cacgctgat ttggctcgt tttggtcat tctgccgtt acttttgag ggttcgttg tacatttat ggagggttt gacggacaa atgttgtcc gatatgtta aaatataca cccgggagt tgtacaaat tttatcact tccaatgag tgtttgagt aagaagccc ggagttaag tgtgtctgg gatatgcgg aataatttt tgtacggcc attcagaat attccggtg aagaatttg acggttcaa ttggatggg ttgataatc cggtgtccg gaattgagt cgtacgatg attacgcaa agtttaatc cagaatgag aatcgttgt gagaaattt gaagattgt ttgagttgc gttcaaacg acgagcgag tgcgtatgg tgtggaaat tcctgtatg cacaacaaa aaatgtcga aatagctcc gaggccaat attgcgaaa ttggaacaa tgtccgctc gatcctagt cctatttgt aagcagctg cacacttgc tcggcttgc acctcgcaa ccgttctgc agatggcgc ttcgagcac gccaaatgc gtgacccac cccttgcct tccaacgta accgccgcc gctgcggaa cccatccaa tgccaaaaa gtctgttcc gaatacaat tcctgtctc aactgcact caggaggag tgcatctgg tgccagaac gaaggccgt tgcgttgac aaaaacgcc tacacggcc agcttcccc tatggtcag tgtcgcgaa tggacaacc gttggaagc aaatgtcgc actcgaggc gcaaacgaa aactcccaa tgcagtttc tactccacg tgtgcaaaa tgtcgcgat gaccccgct tgtggatgg tgcgatgat gggtccagg acaggtctt ggcaaatgt atgcctgga ggatatgct ggtccaaca ttacacact cattcgctc ccttcgtcc acttgtcct ccagatcgg tggcatttt acaacgtgt ccgttgtgt caatgtaat ggacatgcg agttgtatt gcgaattcg agtgtttgt ctaccgtgt cagaatctg acgatgggg acgcagtgt gagcattgt gtcaaaggg tattggggg aatcccgta aatggggga aaatgccaa ccgtgtgaa tgtaacggg caggcgacg cattgccat ccagaatcg ggcaagtgt tattgcacg acaaaggga ttgattggg gatcattgt gagaagtgt gatgccact aatcactat aattccgac ccgattaac aaggattcg tgttattac gatttaaca atcgactat cagttcacc ttcaacctg tcgaagaaa gaagatcgc cactttacc caaataaat ttccgaaat tccccgatt aaggccgac atcgatgcc gatttttcc ataacgtgt tccgttctt gccaaaatg aacataaca ataagacgt ggtggttca aaggaagag aagccgatt tatgaagat ttcaattgt accactttc cggacgaaa ttccctaag tccgattat aatttcggt gttgaggat aacatcacc ttgaccacg ttctacgtt tatgtttac gatttccag ccgccactt tggatccag atttctttc tcccagtac ccgaaatta aatttgcag cagtttttt atcactttt tcgtcgtgt tttctcctg ttgttgctt ttcgctgcg attttgtgg aagattaag cagaggtat gatatgtac cgtcgcaga cagcgactt tttgttgag atggaacaa atggcatcg cgtccgttt tcgcaagtt ttggtcgag ttggaagtg aaggaaaag ctcgatttt gataatatt tcggtgatt gatttgaag cgaaaaaag cgcgatgcc ccaagtcct attgcactt gagccttgt tatggtaat aaagcggcc gttttgagt cttttagtg aggttaccg acgggttgt gagccttac acagttaag ggccagtcg gccggtttg gctatagct agtgctttg gtaacgctg gggaatccc cgcaaaatg agcatcgat caagtgaaa accgaaacg aaaagtgga aaatcgcgc aaatcccaa agtcagcat cctgatagt tgtctttag
- >TC012834 RA
gccttccgc cagttgaca tttcatctg ttatgtagt taattgtca aaaaaatat ggaaaatag tatctctct gaaaaaacc gtcacccct tggccaaaa aagcaccga aatgaagaa accatcacc taatcatta acacccgtt ctaatctcg catcgataa ttccgccac tcgtccatt ttcaattaa aatttaccg ttttgtttt gacgtcgac gtaatgcaa ctgtaaagt aatggtggt aataagatg ttagaaatt ttccaaatg tttttgttc ttgttcaag tcgaaatac cgtcgaaaa tgttacgtg tggcaggct ttgtgttgt gctgttgtg gttttaaat gtttgctct cttgttaag gccaaatgt gcggattgt agcggcaat ggggtgtgt aaaaatgag acttgtgtg tgttacgac ggctggcag gggccccag tgccagttt tgcggcgga aaagtgcgt ttgggtacc tccaccggt accattcac gacggcttg ggcaactac tctctcagc gtgaaatgc tcctggttg atcgacgcc cccaacacc tccatcacc ctccacctg gaggagttc gccacggag tgcggttgg gaccacctg tacatcttc gacggcgac agcgtggac tcgcccctt ctggccgtc ttcagcggt ctcatgtac aaaggcgac tacaacatc cgccgcatc ccagaaatc gtcgcttat tcgggctcc gccctcgtc catttcttc agcgacgat gctttcaac atgtctggc ttcaacatg acctaccgc ctcaacgcc tgtccctcg aaaatatcg ggggtggac tgcagcggc aacggcatt tgcattgat caagtgtgc acatgcaac gggggctgg acgggggtg gcatgccac cttcaaaaa tgccccgat aattgcgga caacacgac ggcagaggc cgctgtgag cccgaattc gggtgccgg tgtgaaagt gactacaag ggggacgat tgcagtcaa tacgccccc agtggctat tgggaaacg gtcaatgtt gaaagtttt gtgcctccg ggatcggcc tcacatggg gctgccgtg tggagggac tccatgtac gtcatcgcc ggggagagc tacagtcgc agatttatg ctctatgta tacgatttt aacgggaac gtctgggag acaccatac atttccgaa agtccctcc caacgctac ggtcactcg accgtcatc tatggcgat aaaatcttc ctctatggt ggtgtgatg ggtaatcgg gggcccacg tccgaagtg tgggctttt gatattaat gccaaaact tgggagaat ataactgtg aaagcagtg gcgtgcaat tcgagtgtg ttgatgtgt gggccgttg aaatcggcc ggacacaca gccactgtg attgagaat tcgaccaat cggaaagcc gatcgtatg attgtttta ttcggtcat tcgccacat cttggctac ttgaatacg gtccaggag tattatttt ggtacacgg gagtggcac atagttgca actaaggga tatccggtc aagggtggc tatggacat acagccacc tgggatccc ttgactggg aaaatttat gtttatgga ggagtggtt tcggaaaat gagtcggct caagttttg agtaaaagt ctttattcg tatgatccc aatacgcga gtttgggtg ttgttgaag gacgcgcct tcggctcgt ttcctccac acttcgact tttatttcg gggggttta atgctagtt tttggggga aatacccac aacgatacg ttgcacagt ttcggagca aagtgttac agttcggag tttttggct tatgacgtt acgtgtgat acttggcag gtgttacaa gtgccaaag gatattcac gctgatttg gctcgtttt ggtcattct gccgttact tttgagggt tcgttgtac atttatgga gggtttgac ggacaaatg ttgtccgat atgttaaaa tatacaccc gggagttgt acaaatttt atcacttcc aatgagtgt ttgagtaag aagcccgga gttaagtgt gtctgggat atgcggaat aatttttgt acggccatt cagaatatt ccggtgaag aatttgacg gttcaattg gatgggttg ataatccgg tgtccggaa ttgagtcgt acgatgatt acgcaaagt ttaatccag aatgagaat cgttgtgag aaatttgaa gattgtttg agttgcgtt caaacgacg agcgagtgc gtatggtgt ggaaattcc tgtatgcac aacaaaaaa tgtcgaaat agctccgag gccaatatt gcgaaattg gaacaatgt ccgctcgat cctagtcct atttgtaag cagctgcac acttgctcg gcttgcacc tcgcaaccg ttctgcaga tggcgcttc gagcacgcc aaatgcgtg acccacccc ttgccttcc aacgtaacc gccgccgct gcggaaccc atccaatgc caaaaagtc tgttccgaa tacaattcc tgtctcaac tgcactcag gaggagtgc atctggtgc cagaacgaa ggccgttgc gttgacaaa aacgcctac acggccagc ttcccctat ggtcagtgt cgcgaatgg acaaccgtt ggaagcaaa tgtcgcact cgaggcgca aacgaaaac tcccaatgc agtttctac tccacgtgt gcaaaatgt cgcgatgac cccgcttgt ggatggtgc gatgatggg tccaggaca ggtcttggc aaatgtatg cctggagga tatgctggt ccaacatta cacactcat tcgctccct tcgtccact tgtcctcca gatcggtgg cattttaca acgtgtccg ttgtgtcaa tgtaatgga catgcgagt tgtattgcg aattcgagt gtttgtcta ccgtgtcag aatctgacg atggggacg cagtgtgag cattgtgtc aaagggtat tgggggaat cccgtaaat gggggaaaa tgccaaccg tgtgaatgt aacgggcag gcgacgcat tgccatcca gaatcgggc aagtgttat tgcacgaca aagggattg attggggat cattgtgag aagtgtgat gccactaat cactataat tccgacccg attaacaag gattcgtgt tattacgat ttaacaatc gactatcag ttcaccttc aacctgtcg aagaaagaa gatcgccac tttacccaa ataaatttc cgaaattcc ccgattaag gccgacatc gatgccgat ttttccata acgtgttcc gttcttgcc aaaatgaac ataacaata agacgtggt ggttcaaag gaagagaag ccgatttat gaagatttc aattgtacc actttccgg acgaaattc cctaagtcc gattataat ttcggtgtt gaggataac atcaccttg accacgttc tacgtttat gtttacgat ttccagccg ccactttgg atccagatt tctttctcc cagtacccg aaattaaat ttgcagcag ttttttatc actttttcg tcgtgtttt ctcctgttg ttgcttttc gctgcgatt ttgtggaag attaagcag aggtatgat atgtaccgt cgcagacag cgacttttt gttgagatg gaacaaatg gcatcgcgt ccgttttcg caagttttg gtcgagttg gaagtgaag gaaaagctc gattttgat aatatttcg gtgattgat ttgaagcga aaaaagcgc gatgcccca agtcctatt gcacttgag ccttgttat ggtaataaa gcggccgtt ttgagtctt ttagtgagg ttaccgacg ggttgtgag ccttacaca gttaagggc cagtcggcc ggtttggct atagctagt gctttggta acgctgggg aatccccgc aaaatgagc atcgatcaa gtgaaaacc gaaacgaaa agtggaaaa tcgcgcaaa tcccaaagt cagcatcct gatagttgt ctttagttg gggtttttt gccgtactt attggtccg aatttgtga tacgttttt ccaggccat ggatttttt taatagcat taaaaatga caaacatga aatagttat tggtgtgtt taggacgcc acttgtatt tcgaagcaa ttttagcaa tattatgat gactaactg cttttttta aaatcgcct cttttcctc ctccatttg tttgtgtgg tgagctttt ttaagacgt tttaatgag agactgaac atatttatt gttttatac tattgatat tttttcatc tgttgattt ttacggtaa tgggctcga attttattt actcatcgg atcgactag tccttgaat catataatg cggtcctga tctcattac gtacacttt actcaaatt ttaggaaac gtgtatccg ttttcaacc cgtctcatt tttatatca atacattca acttagatg caacatatt ttagtaact ttccatttt atttactgc catgtacct attatatta tgtacaatt atttctatt gtaaataaa tcattagtt atatg
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
