Status
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Gene information
General information
TC012834 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15812123..15817281
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012834
    iBeetle screen iB_05331

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 42.86% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Pupal morphology
    wings potentially slightlyshortened - Number of animals showing the phenotype: 3
    wings potentially slightlynot closed - Number of animals showing the phenotype: 3

    Adult morphology
    wings not closed - Number of animals showing the phenotype: 3


    wings surface rough - Number of animals showing the phenotype: 5


    abdominal odoriferous glands partially content not present - Number of animals showing the phenotype: 3 (41 dpi)
    Days post injection



    abdominal odoriferous glands partially colour darker - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012834 RA
      MFLFLFKSK YRRKCYVWQ ALCCAVVVL NVCSLVKAK 
      CADCSGNGV CKNETCVCY DGWQGPQCQ FCGGKVRLG 
      TSTGTIHDG LGNYSLSVK CSWLIDAPN TSITLHLEE 
      FATECGWDH LYIFDGDSV DSPLLAVFS GLMYKGDYN 
      IRRIPEIVA YSGSALVHF FSDDAFNMS GFNMTYRLN 
      ACPSKISGV DCSGNGICI DQVCTCNGG WTGVACHLQ 
      KCPDNCGQH DGRGRCEPE FGCRCESDY KGDDCSQYA 
      PSGYWETVN VESFVPPGS ASHGAAVWR DSMYVIAGE 
      SYSRRFMLY VYDFNGNVW ETPYISESP SQRYGHSTV 
      IYGDKIFLY GGVMGNRGP TSEVWAFDI NAKTWENIT 
      VKAVACNSS VLMCGPLKS AGHTATVIE NSTNRKADR 
      MIVLFGHSP HLGYLNTVQ EYYFGTREW HIVATKGYP 
      VKGGYGHTA TWDPLTGKI YVYGGVVSE NESAQVLSK 
      SLYSYDPNT RVWVLLKDA PSARFLHTS TFISGGLML 
      VFGGNTHND TLHSFGAKC YSSEFLAYD VTCDTWQVL 
      QVPKDIHAD LARFGHSAV TFEGSLYIY GGFDGQMLS 
      DMLKYTPGS CTNFITSNE CLSKKPGVK CVWDMRNNF 
      CTAIQNIPV KNLTVQLDG LIIRCPELS RTMITQSLI 
      QNENRCEKF EDCLSCVQT TSECVWCGN SCMHNKKCR 
      NSSEANIAK LEQCPLDPS PICKQLHTC SACTSQPFC 
      RWRFEHAKC VTHPLPSNV TAAAAEPIQ CQKVCSEYN 
      SCLNCTQEE CIWCQNEGR CVDKNAYTA SFPYGQCRE 
      WTTVGSKCR TRGANENSQ CSFYSTCAK CRDDPACGW 
      CDDGSRTGL GKCMPGGYA GPTLHTHSL PSSTCPPDR 
      WHFTTCPLC QCNGHASCI ANSSVCLPC QNLTMGTQC 
      EHCVKGYWG NPVNGGKCQ PCECNGQAT HCHPESGKC 
      YCTTKGLIG DHCEKCDAT NHYNSDPIN KDSCYYDLT 
      IDYQFTFNL SKKEDRHFT QINFRNSPI KADIDADFS 
      ITCSVLAKM NITIRRGGS KEEKPIYED FNCTTFRTK 
      FPKSDYNFG VEDNITLTT FYVYVYDFQ PPLWIQISF 
      SQYPKLNLQ QFFITFSSC FLLLLLFAA ILWKIKQRY 
      DMYRRRQRL FVEMEQMAS RPFSQVLVE LEVKEKLDF 
      DNISVIDLK RKKRDAPSP IALEPCYGN KAAVLSLLV 
      RLPTGCEPY TVKGQSAGL AIASALVTL GNPRKMSID 
      QVKTETKSG KSRKSQSQH PDSCL
    CDS sequence
    • >TC012834 RA
      atgtttttg ttcttgttc aagtcgaaa taccgtcga 
      aaatgttac gtgtggcag gctttgtgt tgtgctgtt 
      gtggtttta aatgtttgc tctcttgtt aaggccaaa 
      tgtgcggat tgtagcggc aatggggtg tgtaaaaat 
      gagacttgt gtgtgttac gacggctgg caggggccc 
      cagtgccag ttttgcggc ggaaaagtg cgtttgggt 
      acctccacc ggtaccatt cacgacggc ttgggcaac 
      tactctctc agcgtgaaa tgctcctgg ttgatcgac 
      gcccccaac acctccatc accctccac ctggaggag 
      ttcgccacg gagtgcggt tgggaccac ctgtacatc 
      ttcgacggc gacagcgtg gactcgccc cttctggcc 
      gtcttcagc ggtctcatg tacaaaggc gactacaac 
      atccgccgc atcccagaa atcgtcgct tattcgggc 
      tccgccctc gtccatttc ttcagcgac gatgctttc 
      aacatgtct ggcttcaac atgacctac cgcctcaac 
      gcctgtccc tcgaaaata tcgggggtg gactgcagc 
      ggcaacggc atttgcatt gatcaagtg tgcacatgc 
      aacgggggc tggacgggg gtggcatgc caccttcaa 
      aaatgcccc gataattgc ggacaacac gacggcaga 
      ggccgctgt gagcccgaa ttcgggtgc cggtgtgaa 
      agtgactac aagggggac gattgcagt caatacgcc 
      cccagtggc tattgggaa acggtcaat gttgaaagt 
      tttgtgcct ccgggatcg gcctcacat ggggctgcc 
      gtgtggagg gactccatg tacgtcatc gccggggag 
      agctacagt cgcagattt atgctctat gtatacgat 
      tttaacggg aacgtctgg gagacacca tacatttcc 
      gaaagtccc tcccaacgc tacggtcac tcgaccgtc 
      atctatggc gataaaatc ttcctctat ggtggtgtg 
      atgggtaat cgggggccc acgtccgaa gtgtgggct 
      tttgatatt aatgccaaa acttgggag aatataact 
      gtgaaagca gtggcgtgc aattcgagt gtgttgatg 
      tgtgggccg ttgaaatcg gccggacac acagccact 
      gtgattgag aattcgacc aatcggaaa gccgatcgt 
      atgattgtt ttattcggt cattcgcca catcttggc 
      tacttgaat acggtccag gagtattat tttggtaca 
      cgggagtgg cacatagtt gcaactaag ggatatccg 
      gtcaagggt ggctatgga catacagcc acctgggat 
      cccttgact gggaaaatt tatgtttat ggaggagtg 
      gtttcggaa aatgagtcg gctcaagtt ttgagtaaa 
      agtctttat tcgtatgat cccaatacg cgagtttgg 
      gtgttgttg aaggacgcg ccttcggct cgtttcctc 
      cacacttcg acttttatt tcggggggt ttaatgcta 
      gtttttggg ggaaatacc cacaacgat acgttgcac 
      agtttcgga gcaaagtgt tacagttcg gagtttttg 
      gcttatgac gttacgtgt gatacttgg caggtgtta 
      caagtgcca aaggatatt cacgctgat ttggctcgt 
      tttggtcat tctgccgtt acttttgag ggttcgttg 
      tacatttat ggagggttt gacggacaa atgttgtcc 
      gatatgtta aaatataca cccgggagt tgtacaaat 
      tttatcact tccaatgag tgtttgagt aagaagccc 
      ggagttaag tgtgtctgg gatatgcgg aataatttt 
      tgtacggcc attcagaat attccggtg aagaatttg 
      acggttcaa ttggatggg ttgataatc cggtgtccg 
      gaattgagt cgtacgatg attacgcaa agtttaatc 
      cagaatgag aatcgttgt gagaaattt gaagattgt 
      ttgagttgc gttcaaacg acgagcgag tgcgtatgg 
      tgtggaaat tcctgtatg cacaacaaa aaatgtcga 
      aatagctcc gaggccaat attgcgaaa ttggaacaa 
      tgtccgctc gatcctagt cctatttgt aagcagctg 
      cacacttgc tcggcttgc acctcgcaa ccgttctgc 
      agatggcgc ttcgagcac gccaaatgc gtgacccac 
      cccttgcct tccaacgta accgccgcc gctgcggaa 
      cccatccaa tgccaaaaa gtctgttcc gaatacaat 
      tcctgtctc aactgcact caggaggag tgcatctgg 
      tgccagaac gaaggccgt tgcgttgac aaaaacgcc 
      tacacggcc agcttcccc tatggtcag tgtcgcgaa 
      tggacaacc gttggaagc aaatgtcgc actcgaggc 
      gcaaacgaa aactcccaa tgcagtttc tactccacg 
      tgtgcaaaa tgtcgcgat gaccccgct tgtggatgg 
      tgcgatgat gggtccagg acaggtctt ggcaaatgt 
      atgcctgga ggatatgct ggtccaaca ttacacact 
      cattcgctc ccttcgtcc acttgtcct ccagatcgg 
      tggcatttt acaacgtgt ccgttgtgt caatgtaat 
      ggacatgcg agttgtatt gcgaattcg agtgtttgt 
      ctaccgtgt cagaatctg acgatgggg acgcagtgt 
      gagcattgt gtcaaaggg tattggggg aatcccgta 
      aatggggga aaatgccaa ccgtgtgaa tgtaacggg 
      caggcgacg cattgccat ccagaatcg ggcaagtgt 
      tattgcacg acaaaggga ttgattggg gatcattgt 
      gagaagtgt gatgccact aatcactat aattccgac 
      ccgattaac aaggattcg tgttattac gatttaaca 
      atcgactat cagttcacc ttcaacctg tcgaagaaa 
      gaagatcgc cactttacc caaataaat ttccgaaat 
      tccccgatt aaggccgac atcgatgcc gatttttcc 
      ataacgtgt tccgttctt gccaaaatg aacataaca 
      ataagacgt ggtggttca aaggaagag aagccgatt 
      tatgaagat ttcaattgt accactttc cggacgaaa 
      ttccctaag tccgattat aatttcggt gttgaggat 
      aacatcacc ttgaccacg ttctacgtt tatgtttac 
      gatttccag ccgccactt tggatccag atttctttc 
      tcccagtac ccgaaatta aatttgcag cagtttttt 
      atcactttt tcgtcgtgt tttctcctg ttgttgctt 
      ttcgctgcg attttgtgg aagattaag cagaggtat 
      gatatgtac cgtcgcaga cagcgactt tttgttgag 
      atggaacaa atggcatcg cgtccgttt tcgcaagtt 
      ttggtcgag ttggaagtg aaggaaaag ctcgatttt 
      gataatatt tcggtgatt gatttgaag cgaaaaaag 
      cgcgatgcc ccaagtcct attgcactt gagccttgt 
      tatggtaat aaagcggcc gttttgagt cttttagtg 
      aggttaccg acgggttgt gagccttac acagttaag 
      ggccagtcg gccggtttg gctatagct agtgctttg 
      gtaacgctg gggaatccc cgcaaaatg agcatcgat 
      caagtgaaa accgaaacg aaaagtgga aaatcgcgc 
      aaatcccaa agtcagcat cctgatagt tgtctttag
    mRNA sequence
    • >TC012834 RA
      gccttccgc cagttgaca tttcatctg ttatgtagt 
      taattgtca aaaaaatat ggaaaatag tatctctct 
      gaaaaaacc gtcacccct tggccaaaa aagcaccga 
      aatgaagaa accatcacc taatcatta acacccgtt 
      ctaatctcg catcgataa ttccgccac tcgtccatt 
      ttcaattaa aatttaccg ttttgtttt gacgtcgac 
      gtaatgcaa ctgtaaagt aatggtggt aataagatg 
      ttagaaatt ttccaaatg tttttgttc ttgttcaag 
      tcgaaatac cgtcgaaaa tgttacgtg tggcaggct 
      ttgtgttgt gctgttgtg gttttaaat gtttgctct 
      cttgttaag gccaaatgt gcggattgt agcggcaat 
      ggggtgtgt aaaaatgag acttgtgtg tgttacgac 
      ggctggcag gggccccag tgccagttt tgcggcgga 
      aaagtgcgt ttgggtacc tccaccggt accattcac 
      gacggcttg ggcaactac tctctcagc gtgaaatgc 
      tcctggttg atcgacgcc cccaacacc tccatcacc 
      ctccacctg gaggagttc gccacggag tgcggttgg 
      gaccacctg tacatcttc gacggcgac agcgtggac 
      tcgcccctt ctggccgtc ttcagcggt ctcatgtac 
      aaaggcgac tacaacatc cgccgcatc ccagaaatc 
      gtcgcttat tcgggctcc gccctcgtc catttcttc 
      agcgacgat gctttcaac atgtctggc ttcaacatg 
      acctaccgc ctcaacgcc tgtccctcg aaaatatcg 
      ggggtggac tgcagcggc aacggcatt tgcattgat 
      caagtgtgc acatgcaac gggggctgg acgggggtg 
      gcatgccac cttcaaaaa tgccccgat aattgcgga 
      caacacgac ggcagaggc cgctgtgag cccgaattc 
      gggtgccgg tgtgaaagt gactacaag ggggacgat 
      tgcagtcaa tacgccccc agtggctat tgggaaacg 
      gtcaatgtt gaaagtttt gtgcctccg ggatcggcc 
      tcacatggg gctgccgtg tggagggac tccatgtac 
      gtcatcgcc ggggagagc tacagtcgc agatttatg 
      ctctatgta tacgatttt aacgggaac gtctgggag 
      acaccatac atttccgaa agtccctcc caacgctac 
      ggtcactcg accgtcatc tatggcgat aaaatcttc 
      ctctatggt ggtgtgatg ggtaatcgg gggcccacg 
      tccgaagtg tgggctttt gatattaat gccaaaact 
      tgggagaat ataactgtg aaagcagtg gcgtgcaat 
      tcgagtgtg ttgatgtgt gggccgttg aaatcggcc 
      ggacacaca gccactgtg attgagaat tcgaccaat 
      cggaaagcc gatcgtatg attgtttta ttcggtcat 
      tcgccacat cttggctac ttgaatacg gtccaggag 
      tattatttt ggtacacgg gagtggcac atagttgca 
      actaaggga tatccggtc aagggtggc tatggacat 
      acagccacc tgggatccc ttgactggg aaaatttat 
      gtttatgga ggagtggtt tcggaaaat gagtcggct 
      caagttttg agtaaaagt ctttattcg tatgatccc 
      aatacgcga gtttgggtg ttgttgaag gacgcgcct 
      tcggctcgt ttcctccac acttcgact tttatttcg 
      gggggttta atgctagtt tttggggga aatacccac 
      aacgatacg ttgcacagt ttcggagca aagtgttac 
      agttcggag tttttggct tatgacgtt acgtgtgat 
      acttggcag gtgttacaa gtgccaaag gatattcac 
      gctgatttg gctcgtttt ggtcattct gccgttact 
      tttgagggt tcgttgtac atttatgga gggtttgac 
      ggacaaatg ttgtccgat atgttaaaa tatacaccc 
      gggagttgt acaaatttt atcacttcc aatgagtgt 
      ttgagtaag aagcccgga gttaagtgt gtctgggat 
      atgcggaat aatttttgt acggccatt cagaatatt 
      ccggtgaag aatttgacg gttcaattg gatgggttg 
      ataatccgg tgtccggaa ttgagtcgt acgatgatt 
      acgcaaagt ttaatccag aatgagaat cgttgtgag 
      aaatttgaa gattgtttg agttgcgtt caaacgacg 
      agcgagtgc gtatggtgt ggaaattcc tgtatgcac 
      aacaaaaaa tgtcgaaat agctccgag gccaatatt 
      gcgaaattg gaacaatgt ccgctcgat cctagtcct 
      atttgtaag cagctgcac acttgctcg gcttgcacc 
      tcgcaaccg ttctgcaga tggcgcttc gagcacgcc 
      aaatgcgtg acccacccc ttgccttcc aacgtaacc 
      gccgccgct gcggaaccc atccaatgc caaaaagtc 
      tgttccgaa tacaattcc tgtctcaac tgcactcag 
      gaggagtgc atctggtgc cagaacgaa ggccgttgc 
      gttgacaaa aacgcctac acggccagc ttcccctat 
      ggtcagtgt cgcgaatgg acaaccgtt ggaagcaaa 
      tgtcgcact cgaggcgca aacgaaaac tcccaatgc 
      agtttctac tccacgtgt gcaaaatgt cgcgatgac 
      cccgcttgt ggatggtgc gatgatggg tccaggaca 
      ggtcttggc aaatgtatg cctggagga tatgctggt 
      ccaacatta cacactcat tcgctccct tcgtccact 
      tgtcctcca gatcggtgg cattttaca acgtgtccg 
      ttgtgtcaa tgtaatgga catgcgagt tgtattgcg 
      aattcgagt gtttgtcta ccgtgtcag aatctgacg 
      atggggacg cagtgtgag cattgtgtc aaagggtat 
      tgggggaat cccgtaaat gggggaaaa tgccaaccg 
      tgtgaatgt aacgggcag gcgacgcat tgccatcca 
      gaatcgggc aagtgttat tgcacgaca aagggattg 
      attggggat cattgtgag aagtgtgat gccactaat 
      cactataat tccgacccg attaacaag gattcgtgt 
      tattacgat ttaacaatc gactatcag ttcaccttc 
      aacctgtcg aagaaagaa gatcgccac tttacccaa 
      ataaatttc cgaaattcc ccgattaag gccgacatc 
      gatgccgat ttttccata acgtgttcc gttcttgcc 
      aaaatgaac ataacaata agacgtggt ggttcaaag 
      gaagagaag ccgatttat gaagatttc aattgtacc 
      actttccgg acgaaattc cctaagtcc gattataat 
      ttcggtgtt gaggataac atcaccttg accacgttc 
      tacgtttat gtttacgat ttccagccg ccactttgg 
      atccagatt tctttctcc cagtacccg aaattaaat 
      ttgcagcag ttttttatc actttttcg tcgtgtttt 
      ctcctgttg ttgcttttc gctgcgatt ttgtggaag 
      attaagcag aggtatgat atgtaccgt cgcagacag 
      cgacttttt gttgagatg gaacaaatg gcatcgcgt 
      ccgttttcg caagttttg gtcgagttg gaagtgaag 
      gaaaagctc gattttgat aatatttcg gtgattgat 
      ttgaagcga aaaaagcgc gatgcccca agtcctatt 
      gcacttgag ccttgttat ggtaataaa gcggccgtt 
      ttgagtctt ttagtgagg ttaccgacg ggttgtgag 
      ccttacaca gttaagggc cagtcggcc ggtttggct 
      atagctagt gctttggta acgctgggg aatccccgc 
      aaaatgagc atcgatcaa gtgaaaacc gaaacgaaa 
      agtggaaaa tcgcgcaaa tcccaaagt cagcatcct 
      gatagttgt ctttagttg gggtttttt gccgtactt 
      attggtccg aatttgtga tacgttttt ccaggccat 
      ggatttttt taatagcat taaaaatga caaacatga 
      aatagttat tggtgtgtt taggacgcc acttgtatt 
      tcgaagcaa ttttagcaa tattatgat gactaactg 
      cttttttta aaatcgcct cttttcctc ctccatttg 
      tttgtgtgg tgagctttt ttaagacgt tttaatgag 
      agactgaac atatttatt gttttatac tattgatat 
      tttttcatc tgttgattt ttacggtaa tgggctcga 
      attttattt actcatcgg atcgactag tccttgaat 
      catataatg cggtcctga tctcattac gtacacttt 
      actcaaatt ttaggaaac gtgtatccg ttttcaacc 
      cgtctcatt tttatatca atacattca acttagatg 
      caacatatt ttagtaact ttccatttt atttactgc 
      catgtacct attatatta tgtacaatt atttctatt 
      gtaaataaa tcattagtt atatg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY