Status
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Gene information
General information
TC012834 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15812123..15817281
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC012834
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05331

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 42.86% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    Pupal morphology
    wings potentially slightlyshortened - Number of animals showing the phenotype: 3
    wings potentially slightlynot closed - Number of animals showing the phenotype: 3

    Adult morphology
    wings not closed - Number of animals showing the phenotype: 3


    wings surface rough - Number of animals showing the phenotype: 5


    abdominal odoriferous glands partially content not present - Number of animals showing the phenotype: 3 (41 dpi)
    Days post injection


    abdominal odoriferous glands partially colour darker - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012834 RA
      MFLFLFKSK YRRKCYVWQ ALCCAVVVL NVCSLVKAK 
CADCSGNGV CKNETCVCY DGWQGPQCQ FCGGKVRLG 
TSTGTIHDG LGNYSLSVK CSWLIDAPN TSITLHLEE 
FATECGWDH LYIFDGDSV DSPLLAVFS GLMYKGDYN 
IRRIPEIVA YSGSALVHF FSDDAFNMS GFNMTYRLN 
ACPSKISGV DCSGNGICI DQVCTCNGG WTGVACHLQ 
KCPDNCGQH DGRGRCEPE FGCRCESDY KGDDCSQYA 
PSGYWETVN VESFVPPGS ASHGAAVWR DSMYVIAGE 
SYSRRFMLY VYDFNGNVW ETPYISESP SQRYGHSTV 
IYGDKIFLY GGVMGNRGP TSEVWAFDI NAKTWENIT 
VKAVACNSS VLMCGPLKS AGHTATVIE NSTNRKADR 
MIVLFGHSP HLGYLNTVQ EYYFGTREW HIVATKGYP 
VKGGYGHTA TWDPLTGKI YVYGGVVSE NESAQVLSK 
SLYSYDPNT RVWVLLKDA PSARFLHTS TFISGGLML 
VFGGNTHND TLHSFGAKC YSSEFLAYD VTCDTWQVL 
QVPKDIHAD LARFGHSAV TFEGSLYIY GGFDGQMLS 
DMLKYTPGS CTNFITSNE CLSKKPGVK CVWDMRNNF 
CTAIQNIPV KNLTVQLDG LIIRCPELS RTMITQSLI 
QNENRCEKF EDCLSCVQT TSECVWCGN SCMHNKKCR 
NSSEANIAK LEQCPLDPS PICKQLHTC SACTSQPFC 
RWRFEHAKC VTHPLPSNV TAAAAEPIQ CQKVCSEYN 
SCLNCTQEE CIWCQNEGR CVDKNAYTA SFPYGQCRE 
WTTVGSKCR TRGANENSQ CSFYSTCAK CRDDPACGW 
CDDGSRTGL GKCMPGGYA GPTLHTHSL PSSTCPPDR 
WHFTTCPLC QCNGHASCI ANSSVCLPC QNLTMGTQC 
EHCVKGYWG NPVNGGKCQ PCECNGQAT HCHPESGKC 
YCTTKGLIG DHCEKCDAT NHYNSDPIN KDSCYYDLT 
IDYQFTFNL SKKEDRHFT QINFRNSPI KADIDADFS 
ITCSVLAKM NITIRRGGS KEEKPIYED FNCTTFRTK 
FPKSDYNFG VEDNITLTT FYVYVYDFQ PPLWIQISF 
SQYPKLNLQ QFFITFSSC FLLLLLFAA ILWKIKQRY 
DMYRRRQRL FVEMEQMAS RPFSQVLVE LEVKEKLDF 
DNISVIDLK RKKRDAPSP IALEPCYGN KAAVLSLLV 
RLPTGCEPY TVKGQSAGL AIASALVTL GNPRKMSID 
QVKTETKSG KSRKSQSQH PDSCL
    CDS sequence
    • >TC012834 RA
      atgtttttg ttcttgttc aagtcgaaa taccgtcga 
aaatgttac gtgtggcag gctttgtgt tgtgctgtt 
gtggtttta aatgtttgc tctcttgtt aaggccaaa 
tgtgcggat tgtagcggc aatggggtg tgtaaaaat 
gagacttgt gtgtgttac gacggctgg caggggccc 
cagtgccag ttttgcggc ggaaaagtg cgtttgggt 
acctccacc ggtaccatt cacgacggc ttgggcaac 
tactctctc agcgtgaaa tgctcctgg ttgatcgac 
gcccccaac acctccatc accctccac ctggaggag 
ttcgccacg gagtgcggt tgggaccac ctgtacatc 
ttcgacggc gacagcgtg gactcgccc cttctggcc 
gtcttcagc ggtctcatg tacaaaggc gactacaac 
atccgccgc atcccagaa atcgtcgct tattcgggc 
tccgccctc gtccatttc ttcagcgac gatgctttc 
aacatgtct ggcttcaac atgacctac cgcctcaac 
gcctgtccc tcgaaaata tcgggggtg gactgcagc 
ggcaacggc atttgcatt gatcaagtg tgcacatgc 
aacgggggc tggacgggg gtggcatgc caccttcaa 
aaatgcccc gataattgc ggacaacac gacggcaga 
ggccgctgt gagcccgaa ttcgggtgc cggtgtgaa 
agtgactac aagggggac gattgcagt caatacgcc 
cccagtggc tattgggaa acggtcaat gttgaaagt 
tttgtgcct ccgggatcg gcctcacat ggggctgcc 
gtgtggagg gactccatg tacgtcatc gccggggag 
agctacagt cgcagattt atgctctat gtatacgat 
tttaacggg aacgtctgg gagacacca tacatttcc 
gaaagtccc tcccaacgc tacggtcac tcgaccgtc 
atctatggc gataaaatc ttcctctat ggtggtgtg 
atgggtaat cgggggccc acgtccgaa gtgtgggct 
tttgatatt aatgccaaa acttgggag aatataact 
gtgaaagca gtggcgtgc aattcgagt gtgttgatg 
tgtgggccg ttgaaatcg gccggacac acagccact 
gtgattgag aattcgacc aatcggaaa gccgatcgt 
atgattgtt ttattcggt cattcgcca catcttggc 
tacttgaat acggtccag gagtattat tttggtaca 
cgggagtgg cacatagtt gcaactaag ggatatccg 
gtcaagggt ggctatgga catacagcc acctgggat 
cccttgact gggaaaatt tatgtttat ggaggagtg 
gtttcggaa aatgagtcg gctcaagtt ttgagtaaa 
agtctttat tcgtatgat cccaatacg cgagtttgg 
gtgttgttg aaggacgcg ccttcggct cgtttcctc 
cacacttcg acttttatt tcggggggt ttaatgcta 
gtttttggg ggaaatacc cacaacgat acgttgcac 
agtttcgga gcaaagtgt tacagttcg gagtttttg 
gcttatgac gttacgtgt gatacttgg caggtgtta 
caagtgcca aaggatatt cacgctgat ttggctcgt 
tttggtcat tctgccgtt acttttgag ggttcgttg 
tacatttat ggagggttt gacggacaa atgttgtcc 
gatatgtta aaatataca cccgggagt tgtacaaat 
tttatcact tccaatgag tgtttgagt aagaagccc 
ggagttaag tgtgtctgg gatatgcgg aataatttt 
tgtacggcc attcagaat attccggtg aagaatttg 
acggttcaa ttggatggg ttgataatc cggtgtccg 
gaattgagt cgtacgatg attacgcaa agtttaatc 
cagaatgag aatcgttgt gagaaattt gaagattgt 
ttgagttgc gttcaaacg acgagcgag tgcgtatgg 
tgtggaaat tcctgtatg cacaacaaa aaatgtcga 
aatagctcc gaggccaat attgcgaaa ttggaacaa 
tgtccgctc gatcctagt cctatttgt aagcagctg 
cacacttgc tcggcttgc acctcgcaa ccgttctgc 
agatggcgc ttcgagcac gccaaatgc gtgacccac 
cccttgcct tccaacgta accgccgcc gctgcggaa 
cccatccaa tgccaaaaa gtctgttcc gaatacaat 
tcctgtctc aactgcact caggaggag tgcatctgg 
tgccagaac gaaggccgt tgcgttgac aaaaacgcc 
tacacggcc agcttcccc tatggtcag tgtcgcgaa 
tggacaacc gttggaagc aaatgtcgc actcgaggc 
gcaaacgaa aactcccaa tgcagtttc tactccacg 
tgtgcaaaa tgtcgcgat gaccccgct tgtggatgg 
tgcgatgat gggtccagg acaggtctt ggcaaatgt 
atgcctgga ggatatgct ggtccaaca ttacacact 
cattcgctc ccttcgtcc acttgtcct ccagatcgg 
tggcatttt acaacgtgt ccgttgtgt caatgtaat 
ggacatgcg agttgtatt gcgaattcg agtgtttgt 
ctaccgtgt cagaatctg acgatgggg acgcagtgt 
gagcattgt gtcaaaggg tattggggg aatcccgta 
aatggggga aaatgccaa ccgtgtgaa tgtaacggg 
caggcgacg cattgccat ccagaatcg ggcaagtgt 
tattgcacg acaaaggga ttgattggg gatcattgt 
gagaagtgt gatgccact aatcactat aattccgac 
ccgattaac aaggattcg tgttattac gatttaaca 
atcgactat cagttcacc ttcaacctg tcgaagaaa 
gaagatcgc cactttacc caaataaat ttccgaaat 
tccccgatt aaggccgac atcgatgcc gatttttcc 
ataacgtgt tccgttctt gccaaaatg aacataaca 
ataagacgt ggtggttca aaggaagag aagccgatt 
tatgaagat ttcaattgt accactttc cggacgaaa 
ttccctaag tccgattat aatttcggt gttgaggat 
aacatcacc ttgaccacg ttctacgtt tatgtttac 
gatttccag ccgccactt tggatccag atttctttc 
tcccagtac ccgaaatta aatttgcag cagtttttt 
atcactttt tcgtcgtgt tttctcctg ttgttgctt 
ttcgctgcg attttgtgg aagattaag cagaggtat 
gatatgtac cgtcgcaga cagcgactt tttgttgag 
atggaacaa atggcatcg cgtccgttt tcgcaagtt 
ttggtcgag ttggaagtg aaggaaaag ctcgatttt 
gataatatt tcggtgatt gatttgaag cgaaaaaag 
cgcgatgcc ccaagtcct attgcactt gagccttgt 
tatggtaat aaagcggcc gttttgagt cttttagtg 
aggttaccg acgggttgt gagccttac acagttaag 
ggccagtcg gccggtttg gctatagct agtgctttg 
gtaacgctg gggaatccc cgcaaaatg agcatcgat 
caagtgaaa accgaaacg aaaagtgga aaatcgcgc 
aaatcccaa agtcagcat cctgatagt tgtctttag
    mRNA sequence
    • >TC012834 RA
      gccttccgc cagttgaca tttcatctg ttatgtagt 
taattgtca aaaaaatat ggaaaatag tatctctct 
gaaaaaacc gtcacccct tggccaaaa aagcaccga 
aatgaagaa accatcacc taatcatta acacccgtt 
ctaatctcg catcgataa ttccgccac tcgtccatt 
ttcaattaa aatttaccg ttttgtttt gacgtcgac 
gtaatgcaa ctgtaaagt aatggtggt aataagatg 
ttagaaatt ttccaaatg tttttgttc ttgttcaag 
tcgaaatac cgtcgaaaa tgttacgtg tggcaggct 
ttgtgttgt gctgttgtg gttttaaat gtttgctct 
cttgttaag gccaaatgt gcggattgt agcggcaat 
ggggtgtgt aaaaatgag acttgtgtg tgttacgac 
ggctggcag gggccccag tgccagttt tgcggcgga 
aaagtgcgt ttgggtacc tccaccggt accattcac 
gacggcttg ggcaactac tctctcagc gtgaaatgc 
tcctggttg atcgacgcc cccaacacc tccatcacc 
ctccacctg gaggagttc gccacggag tgcggttgg 
gaccacctg tacatcttc gacggcgac agcgtggac 
tcgcccctt ctggccgtc ttcagcggt ctcatgtac 
aaaggcgac tacaacatc cgccgcatc ccagaaatc 
gtcgcttat tcgggctcc gccctcgtc catttcttc 
agcgacgat gctttcaac atgtctggc ttcaacatg 
acctaccgc ctcaacgcc tgtccctcg aaaatatcg 
ggggtggac tgcagcggc aacggcatt tgcattgat 
caagtgtgc acatgcaac gggggctgg acgggggtg 
gcatgccac cttcaaaaa tgccccgat aattgcgga 
caacacgac ggcagaggc cgctgtgag cccgaattc 
gggtgccgg tgtgaaagt gactacaag ggggacgat 
tgcagtcaa tacgccccc agtggctat tgggaaacg 
gtcaatgtt gaaagtttt gtgcctccg ggatcggcc 
tcacatggg gctgccgtg tggagggac tccatgtac 
gtcatcgcc ggggagagc tacagtcgc agatttatg 
ctctatgta tacgatttt aacgggaac gtctgggag 
acaccatac atttccgaa agtccctcc caacgctac 
ggtcactcg accgtcatc tatggcgat aaaatcttc 
ctctatggt ggtgtgatg ggtaatcgg gggcccacg 
tccgaagtg tgggctttt gatattaat gccaaaact 
tgggagaat ataactgtg aaagcagtg gcgtgcaat 
tcgagtgtg ttgatgtgt gggccgttg aaatcggcc 
ggacacaca gccactgtg attgagaat tcgaccaat 
cggaaagcc gatcgtatg attgtttta ttcggtcat 
tcgccacat cttggctac ttgaatacg gtccaggag 
tattatttt ggtacacgg gagtggcac atagttgca 
actaaggga tatccggtc aagggtggc tatggacat 
acagccacc tgggatccc ttgactggg aaaatttat 
gtttatgga ggagtggtt tcggaaaat gagtcggct 
caagttttg agtaaaagt ctttattcg tatgatccc 
aatacgcga gtttgggtg ttgttgaag gacgcgcct 
tcggctcgt ttcctccac acttcgact tttatttcg 
gggggttta atgctagtt tttggggga aatacccac 
aacgatacg ttgcacagt ttcggagca aagtgttac 
agttcggag tttttggct tatgacgtt acgtgtgat 
acttggcag gtgttacaa gtgccaaag gatattcac 
gctgatttg gctcgtttt ggtcattct gccgttact 
tttgagggt tcgttgtac atttatgga gggtttgac 
ggacaaatg ttgtccgat atgttaaaa tatacaccc 
gggagttgt acaaatttt atcacttcc aatgagtgt 
ttgagtaag aagcccgga gttaagtgt gtctgggat 
atgcggaat aatttttgt acggccatt cagaatatt 
ccggtgaag aatttgacg gttcaattg gatgggttg 
ataatccgg tgtccggaa ttgagtcgt acgatgatt 
acgcaaagt ttaatccag aatgagaat cgttgtgag 
aaatttgaa gattgtttg agttgcgtt caaacgacg 
agcgagtgc gtatggtgt ggaaattcc tgtatgcac 
aacaaaaaa tgtcgaaat agctccgag gccaatatt 
gcgaaattg gaacaatgt ccgctcgat cctagtcct 
atttgtaag cagctgcac acttgctcg gcttgcacc 
tcgcaaccg ttctgcaga tggcgcttc gagcacgcc 
aaatgcgtg acccacccc ttgccttcc aacgtaacc 
gccgccgct gcggaaccc atccaatgc caaaaagtc 
tgttccgaa tacaattcc tgtctcaac tgcactcag 
gaggagtgc atctggtgc cagaacgaa ggccgttgc 
gttgacaaa aacgcctac acggccagc ttcccctat 
ggtcagtgt cgcgaatgg acaaccgtt ggaagcaaa 
tgtcgcact cgaggcgca aacgaaaac tcccaatgc 
agtttctac tccacgtgt gcaaaatgt cgcgatgac 
cccgcttgt ggatggtgc gatgatggg tccaggaca 
ggtcttggc aaatgtatg cctggagga tatgctggt 
ccaacatta cacactcat tcgctccct tcgtccact 
tgtcctcca gatcggtgg cattttaca acgtgtccg 
ttgtgtcaa tgtaatgga catgcgagt tgtattgcg 
aattcgagt gtttgtcta ccgtgtcag aatctgacg 
atggggacg cagtgtgag cattgtgtc aaagggtat 
tgggggaat cccgtaaat gggggaaaa tgccaaccg 
tgtgaatgt aacgggcag gcgacgcat tgccatcca 
gaatcgggc aagtgttat tgcacgaca aagggattg 
attggggat cattgtgag aagtgtgat gccactaat 
cactataat tccgacccg attaacaag gattcgtgt 
tattacgat ttaacaatc gactatcag ttcaccttc 
aacctgtcg aagaaagaa gatcgccac tttacccaa 
ataaatttc cgaaattcc ccgattaag gccgacatc 
gatgccgat ttttccata acgtgttcc gttcttgcc 
aaaatgaac ataacaata agacgtggt ggttcaaag 
gaagagaag ccgatttat gaagatttc aattgtacc 
actttccgg acgaaattc cctaagtcc gattataat 
ttcggtgtt gaggataac atcaccttg accacgttc 
tacgtttat gtttacgat ttccagccg ccactttgg 
atccagatt tctttctcc cagtacccg aaattaaat 
ttgcagcag ttttttatc actttttcg tcgtgtttt 
ctcctgttg ttgcttttc gctgcgatt ttgtggaag 
attaagcag aggtatgat atgtaccgt cgcagacag 
cgacttttt gttgagatg gaacaaatg gcatcgcgt 
ccgttttcg caagttttg gtcgagttg gaagtgaag 
gaaaagctc gattttgat aatatttcg gtgattgat 
ttgaagcga aaaaagcgc gatgcccca agtcctatt 
gcacttgag ccttgttat ggtaataaa gcggccgtt 
ttgagtctt ttagtgagg ttaccgacg ggttgtgag 
ccttacaca gttaagggc cagtcggcc ggtttggct 
atagctagt gctttggta acgctgggg aatccccgc 
aaaatgagc atcgatcaa gtgaaaacc gaaacgaaa 
agtggaaaa tcgcgcaaa tcccaaagt cagcatcct 
gatagttgt ctttagttg gggtttttt gccgtactt 
attggtccg aatttgtga tacgttttt ccaggccat 
ggatttttt taatagcat taaaaatga caaacatga 
aatagttat tggtgtgtt taggacgcc acttgtatt 
tcgaagcaa ttttagcaa tattatgat gactaactg 
cttttttta aaatcgcct cttttcctc ctccatttg 
tttgtgtgg tgagctttt ttaagacgt tttaatgag 
agactgaac atatttatt gttttatac tattgatat 
tttttcatc tgttgattt ttacggtaa tgggctcga 
attttattt actcatcgg atcgactag tccttgaat 
catataatg cggtcctga tctcattac gtacacttt 
actcaaatt ttaggaaac gtgtatccg ttttcaacc 
cgtctcatt tttatatca atacattca acttagatg 
caacatatt ttagtaact ttccatttt atttactgc 
catgtacct attatatta tgtacaatt atttctatt 
gtaaataaa tcattagtt atatg
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