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Gene information
General information
TC012833 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15821800..15823571
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012833
    iBeetle screen iB_05330

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 22.22% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 22.22% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012833 RA
      MSCEYFSKA ITVSDFDRI CRTCLGDNK TRPIMDTII 
      GDFKAVELL ESCVTLQVT KNDSLPQNI CESCLNELY 
      RFHEFKKQC QDNEAVLKL LLEQPQSEQ KLGEDEEFC 
      NITLIEEEE VEKNETSEE KPKSKTDPK THKCEICDK 
      VFTRSKLTQ HMRTHTKEK PYQCEICLQ SFSMRGNLK 
      RHKMTHTGE RPHVCSTCG RGFIQATSL HAHEKTHTH 
      DDQYARDQT DPMKFLCTY CGRGFKRLV PFNAHLEKH 
      TRTKHHAKK EETPEAREH ICEICSRGY KTKHLLKAH 
      SLTHGEKSF LCSDCGKGF VTKAALQSH LKVHTGEKP 
      HRCIICQKS FAHVGSFDS HMLIHSGLK PYDCSYCPK 
      SFTQLSHLT YHLRTHSGE RPFSCSYCG KSFALKGNL 
      TVHVRTHTG ETPYVCPVC GKGFYDSSS MKKHQRNHR 
      AQEFETARG GEKPDVPNW AESNCPQT
    CDS sequence
    • >TC012833 RA
      atgagttgt gaatacttt tccaaagcc ataactgtg 
      agtgacttt gaccgaatc tgccgcacg tgtctcggg 
      gacaataaa acaaggccc atcatggac acaataatt 
      ggggacttt aaagcagtt gaattactg gagtcttgc 
      gtaacttta caagtgaca aaaaatgat tcgttgcct 
      cagaacata tgtgaatca tgcctgaac gaactttac 
      cgttttcac gagtttaaa aaacagtgt caggataac 
      gaagctgtc ttaaaatta ttgcttgaa cagccacaa 
      tccgagcaa aaactaggt gaagacgaa gaattttgc 
      aatataacg ctaattgag gaggaagaa gttgaaaaa 
      aacgaaact tccgaagaa aaacccaag tctaagact 
      gatccaaaa acccacaag tgtgaaatc tgcgataag 
      gtcttcact cgcagcaag ctcacgcaa cacatgcgc 
      acccacacg aaggaaaag ccctaccaa tgcgaaatc 
      tgcctccag agtttcagt atgcgaggc aatttaaag 
      cgccacaaa atgacacat acgggggaa cgcccccac 
      gtttgctcc acctgtggg cggggtttc atccaagcc 
      acgtcgctc cacgcccac gaaaaaacc cacacacac 
      gacgatcaa tacgcacgt gaccaaacc gacccaatg 
      aaattcctt tgcacgtac tgtgggcgt ggcttcaag 
      cgcctcgtt ccgttcaac gcccacttg gagaaacac 
      actcggacg aaacaccac gccaaaaaa gaggaaact 
      ccagaagcg cgcgaacat atttgcgag atttgttcg 
      cgtggttac aaaaccaaa catttgtta aaagcgcac 
      agtttaacg cacggcgaa aagtcgttt ttatgcagc 
      gattgtggc aaaggtttc gtcacgaaa gcggcgctg 
      cagtcgcac ctcaaagtc cacacgggg gaaaaaccg 
      caccggtgt atcatctgc caaaaatcg tttgctcat 
      gttggcagt tttgactct cacatgtta attcattcc 
      ggtcttaaa ccgtacgat tgtagttat tgtcccaaa 
      tcgttcact caactgtca catttgacc tatcatttg 
      cggacacat agtggcgaa agacccttt tcgtgctcc 
      tactgtggc aaaagtttc gcccttaag ggcaattta 
      acagtacat gtgaggaca cacacgggg gagacgccc 
      tacgtgtgc cccgtttgc ggaaaaggt ttctacgat 
      tcaagcagc atgaaaaaa catcaaagg aaccatagg 
      gctcaagag tttgaaaca gctagaggt ggggaaaag 
      ccggatgta cccaactgg gcggagtct aactgcccg 
      caaacctag
    mRNA sequence
    • >TC012833 RA
      cacgggaag tgaggttac gtgattatt ttatttatc 
      aaaaaaatt caatttccc agcttatac ctacgttag 
      gtgtattaa aactagaaa aagtggagt tggtgtttg 
      aattaaagt gattatctc gtgttattt aacaccttt 
      tcattgata aaatcggtg taagattag tggtcataa 
      aaatggttt tctgttaat tattcggca agaaccaca 
      aaaaatgag ttgtgaata cttttccaa agccataac 
      tgtgagtga ctttgaccg aatctgccg cacgtgtct 
      cggggacaa taaaacaag gcccatcat ggacacaat 
      aattgggga ctttaaagc agttgaatt actggagtc 
      ttgcgtaac tttacaagt gacaaaaaa tgattcgtt 
      gcctcagaa catatgtga atcatgcct gaacgaact 
      ttaccgttt tcacgagtt taaaaaaca gtgtcagga 
      taacgaagc tgtcttaaa attattgct tgaacagcc 
      acaatccga gcaaaaact aggtgaaga cgaagaatt 
      ttgcaatat aacgctaat tgaggagga agaagttga 
      aaaaaacga aacttccga agaaaaacc caagtctaa 
      gactgatcc aaaaaccca caagtgtga aatctgcga 
      taaggtctt cactcgcag caagctcac gcaacacat 
      gcgcaccca cacgaagga aaagcccta ccaatgcga 
      aatctgcct ccagagttt cagtatgcg aggcaattt 
      aaagcgcca caaaatgac acatacggg ggaacgccc 
      ccacgtttg ctccacctg tgggcgggg tttcatcca 
      agccacgtc gctccacgc ccacgaaaa aacccacac 
      acacgacga tcaatacgc acgtgacca aaccgaccc 
      aatgaaatt cctttgcac gtactgtgg gcgtggctt 
      caagcgcct cgttccgtt caacgccca cttggagaa 
      acacactcg gacgaaaca ccacgccaa aaaagagga 
      aactccaga agcgcgcga acatatttg cgagatttg 
      ttcgcgtgg ttacaaaac caaacattt gttaaaagc 
      gcacagttt aacgcacgg cgaaaagtc gtttttatg 
      cagcgattg tggcaaagg tttcgtcac gaaagcggc 
      gctgcagtc gcacctcaa agtccacac gggggaaaa 
      accgcaccg gtgtatcat ctgccaaaa atcgtttgc 
      tcatgttgg cagttttga ctctcacat gttaattca 
      ttccggtct taaaccgta cgattgtag ttattgtcc 
      caaatcgtt cactcaact gtcacattt gacctatca 
      tttgcggac acatagtgg cgaaagacc cttttcgtg 
      ctcctactg tggcaaaag tttcgccct taagggcaa 
      tttaacagt acatgtgag gacacacac gggggagac 
      gccctacgt gtgccccgt ttgcggaaa aggtttcta 
      cgattcaag cagcatgaa aaaacatca aaggaacca 
      tagggctca agagtttga aacagctag aggtgggga 
      aaagccgga tgtacccaa ctgggcgga gtctaactg 
      cccgcaaac ctagacatg tctctaggt cactgattt 
      ttttatcaa ctgtgtaga taaaaaaag tactaaaaa 
      taaatttcg taactagaa gttgtattt tattgcggg 
      ttaagaggt gaatgatac aaaaaaaat cgtctggtg 
      atgacgcgt tgctatggt cgtaacaat acaattgcc 
      atggcatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY