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Gene information
General information
TC012828 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15867941..15869221
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC012828
      iBeetle screen iB_05329

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection


      Pupal morphology
      beetle stock: bad (increased lethality),
      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): less than 30%

      embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

      embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

      larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

      larva appendages shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

      larva appendages shortened - Penetrance (percentage of animals/eggs on slide): less than 30%

      head partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

      head shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

      head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

      thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%

      thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      abdomen partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
      Days post injection


      eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
      Days post injection


      Adult morphology
      abdominal odoriferous glands content darker - Number of animals showing the phenotype: 4 (41 dpi)
      Days post injection



      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC012828 RA
        MKALILVSC VLAAATAQY HHPLYYPQH IPVLDHNGV 
        PVEPAANQL ARAAHYAAH AEANARTGH YPIYAAPVI 
        HNALPADTP EVAAARAQH FADYAVAAQ RNGVHVLPL 
        AHTAAAAHP IDTPEVQLA KAAHFAAHA AARSGLHIR 
        KRRALYHHY PVIGADGVP VNTPEVQAA TAAHLAAHA 
        AVRGVPLDT PEVQAAKAK HFQAHAEAL HRTGAVGIA 
        PYYYGGLYQ PHAVIGHDG HPVETPEVQ LAKAAHFAA 
        HAAARHYY
      CDS sequence
      • >TC012828 RA
        atgaaggct ctgatctta gtaagctgc gtgttggcc 
        gccgccacc gcccaatac caccacccc ctgtactac 
        ccccagcat atccccgtg ctcgaccac aacggcgtc 
        cctgtcgaa cccgccgcc aaccaactc gcccgcgcc 
        gcccattac gccgcccat gccgaagcc aatgctcgc 
        actggtcat tatccaatt tacgccgcc cccgttatc 
        cacaatgct cttcctgcc gacacccct gaagtcgcc 
        gccgccaga gcccaacac tttgctgac tacgctgtt 
        gccgcccaa cgtaacggc gtccatgtt ttaccacta 
        gctcacacc gccgccgcc gcccatccc atcgacacc 
        cccgaagtc caattggcc aaagccgcc cattttgcc 
        gctcacgcc gccgccaga tctggactt cacatccgc 
        aaaagacgc gccttgtac catcattat ccagtgatt 
        ggagctgat ggtgttcca gtcaatact cccgaagtc 
        caggctgct actgctgcc catttggct gcacatgct 
        gctgtgaga ggagttcct ttggatact cccgaagtc 
        caggctgcc aaagctaag catttccaa gcccatgct 
        gaagcttta cacaggact ggagctgtg ggtattgcc 
        ccatattat tatggaggt ttgtaccaa ccccatgct 
        gtgattgga catgatggg catccagtc gaaacgccg 
        gaagttcag ttggctaaa gctgcccat tttgcagcc 
        catgctgcc gccagacac tactactag
      mRNA sequence
      • >TC012828 RA
        gggaaacta tataacact ggcggggct gttgaattt 
        gcacagttg ttcaacagt cctacagtg cttaggagg 
        ttgaaagcg tttgacaat gaaggctct gatcttagt 
        aagctgcgt gttggccgc cgccaccgc ccaatacca 
        ccaccccct gtactaccc ccagcatat ccccgtgct 
        cgaccacaa cggcgtccc tgtcgaacc cgccgccaa 
        ccaactcgc ccgcgccgc ccattacgc cgcccatgc 
        cgaagccaa tgctcgcac tggtcatta tccaattta 
        cgccgcccc cgttatcca caatgctct tcctgccga 
        cacccctga agtcgccgc cgccagagc ccaacactt 
        tgctgacta cgctgttgc cgcccaacg taacggcgt 
        ccatgtttt accactagc tcacaccgc cgccgccgc 
        ccatcccat cgacacccc cgaagtcca attggccaa 
        agccgccca ttttgccgc tcacgccgc cgccagatc 
        tggacttca catccgcaa aagacgcgc cttgtacca 
        tcattatcc agtgattgg agctgatgg tgttccagt 
        caatactcc cgaagtcca ggctgctac tgctgccca 
        tttggctgc acatgctgc tgtgagagg agttccttt 
        ggatactcc cgaagtcca ggctgccaa agctaagca 
        tttccaagc ccatgctga agctttaca caggactgg 
        agctgtggg tattgcccc atattatta tggaggttt 
        gtaccaacc ccatgctgt gattggaca tgatgggca 
        tccagtcga aacgccgga agttcagtt ggctaaagc 
        tgcccattt tgcagccca tgctgccgc cagacacta 
        ctactagat cggccttca agtggtttt ttgttaagg 
        aaaatttgt aaataaaat tttaataaa accattaag 
        aaatt
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY