General information | |||
TC012819 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 15984101..15987771 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC012819 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_05326 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 1 (9 dpi)larval musculature potentially degenerated - Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
head partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
antenna partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
antenna irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
antenna shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
- >TC012819 RA
MLILLLVPL LTGASATSN VTIDEIPET YLNLSAGNS LWTALLKNC RQPTMACVE KTVYEYLKR TVETREDLH VVPFVKMKR NQVDYDRIS GPEIGDWDE EEPESSLEA MSRDLHGKS VKFLMTHDM EVQLPETMF QGSVLKISP RAFEGNGAL VKLEIQPKE VQGIAEGRL FKKLKNFIS EKLIYALLA ILLVIKLLA AKFMFFMPM AVGAVTAKK LLIKVLLFL FPALHHFFK LCAYVPHGT KYHHHKHHI SHIHHVAPH KHHHHGHHH PHPHDSVEV IHPHAEGPP VVEGPWHHG GASLHHSIE IEEPHKTYY KPHEQELEY YSGGPHLSH HFINHRQDS QEDNEVQPA QGHRNKKPS NRPLTPSEI EAMVLKAEK EAKLKVRLQ QERHRIQEE NARLQEQLK LALKIQEKL KQQAAVVKA KLPVPPSTN LQTPASEQK QVYSAKVKQ PEVVQPETK KEPTPFETA LHQAAAITY DPFYSPILE KIDKILVGL GFNEEPCRE RLICSMYKN PVKFSPHSN LLSAELSRD SKELQKPTT TNAAVIRFY RYVQAARDG QDKRECLRL YPSCSINTE Q
- >TC012819 RA
atgttaatt ttgctattg gtgccactt ttgaccgga gcgagcgcg acctccaac gtgaccatc gacgaaata ccagagact tacctcaat ctatcagca ggaaattcc ttatggacg gcactgctg aagaactgc cgccagccg acgatggcc tgcgtcgaa aaaaccgtc tacgagtac ttgaaacgt acggtagag actcgggag gatttgcac gtggtgcca tttgttaaa atgaagcgg aaccaagtg gattacgac cggatttcc gggccggaa atcggagac tgggatgag gaggaaccc gagagcagt ctggaagca atgtccaga gatttgcat gggaaaagt gtcaagttt ttgatgacc catgacatg gaagtgcaa ttaccagaa acgatgttt caaggcagt gttttgaaa atctcgccg agggctttt gagggaaat ggggcttta gtcaagctg gaaattcaa ccgaaagaa gtacaaggg attgcagaa ggaagactc ttcaagaag ttgaaaaac ttcatcagc gagaaactc atctatgcc cttttggcg atactttta gtcattaaa cttctcgcg gccaagttt atgtttttt atgccaatg gccgtgggt gcagtaacg gcaaagaaa cttttaatc aaagtacta cttttcctc ttccctgca ttgcatcac ttcttcaaa ttgtgcgct tacgtacca catggtact aaataccat caccacaag caccacatt tcccatatc catcatgtt gcgccccat aagcaccat catcatggg caccatcac ccacaccct catgactct gtcgaggtt atccaccca catgccgaa gggcctccg gtggttgaa gggccctgg caccatgga ggcgcctcc ttgcaccat tccatcgaa atcgaagag ccccacaag acctactac aaaccccac gagcaagaa ttggagtac tactctgga ggaccacac ttatctcac cattttatc aaccatcgg caagacagc caagaagat aacgaagtg caaccggct caaggccac cgcaataag aaaccatcc aaccgtcca ttaacaccc agcgaaatc gaagcaatg gtactaaaa gccgaaaaa gaagccaaa ctgaaagtt cgcctccag caggaacga caccgcatc caagaggaa aacgcacgc ttgcaggaa caattaaaa ctggccttg aaaatacaa gaaaaactc aaacaacag gcggccgtg gtcaaggca aaattaccg gtaccgccg agtactaat ctacaaacg ccagcaagc gagcaaaaa caagtctat tcggctaaa gtgaaacag ccggaagtt gtacaaccg gaaacgaaa aaagaaccg acgccgttt gaaacggct ctacaccaa gccgctgcg ataacgtac gacccgttc tacagcccc attttagag aaaattgat aagatcttg gtcggcttg ggcttcaac gaggagccg tgcagggaa aggctcatt tgtagcatg tacaaaaat ccggtcaag ttcagtccg cacagtaat ctcctatca gcggaatta agcagagat tccaaagaa ctgcaaaag cccacaaca accaacgct gccgtgatc cgattctat cgctacgtg caagcagcc agagacggc caagacaaa cgtgaatgc ctaagactt tatccatca tgttccata aatacagaa caataa
- >TC012819 RA
ttgccggcc agcagagaa cgtcactat gccctgatt gtcatggtg gtccagttt cggtgtgtg ctaggtgga ctttcggga tgtgatagt gtttaattg tggacaaaa actttaaat ccaaagaca tgttaattt tgctattgg tgccacttt tgaccggag cgagcgcga cctccaacg tgaccatcg acgaaatac cagagactt acctcaatc tatcagcag gaaattcct tatggacgg cactgctga agaactgcc gccagccga cgatggcct gcgtcgaaa aaaccgtct acgagtact tgaaacgta cggtagaga ctcgggagg atttgcacg tggtgccat ttgttaaaa tgaagcgga accaagtgg attacgacc ggatttccg ggccggaaa tcggagact gggatgagg aggaacccg agagcagtc tggaagcaa tgtccagag atttgcatg ggaaaagtg tcaagtttt tgatgaccc atgacatgg aagtgcaat taccagaaa cgatgtttc aaggcagtg ttttgaaaa tctcgccga gggcttttg agggaaatg gggctttag tcaagctgg aaattcaac cgaaagaag tacaaggga ttgcagaag gaagactct tcaagaagt tgaaaaact tcatcagcg agaaactca tctatgccc ttttggcga tacttttag tcattaaac ttctcgcgg ccaagttta tgtttttta tgccaatgg ccgtgggtg cagtaacgg caaagaaac ttttaatca aagtactac ttttcctct tccctgcat tgcatcact tcttcaaat tgtgcgctt acgtaccac atggtacta aataccatc accacaagc accacattt cccatatcc atcatgttg cgccccata agcaccatc atcatgggc accatcacc cacaccctc atgactctg tcgaggtta tccacccac atgccgaag ggcctccgg tggttgaag ggccctggc accatggag gcgcctcct tgcaccatt ccatcgaaa tcgaagagc cccacaaga cctactaca aaccccacg agcaagaat tggagtact actctggag gaccacact tatctcacc attttatca accatcggc aagacagcc aagaagata acgaagtgc aaccggctc aaggccacc gcaataaga aaccatcca accgtccat taacaccca gcgaaatcg aagcaatgg tactaaaag ccgaaaaag aagccaaac tgaaagttc gcctccagc aggaacgac accgcatcc aagaggaaa acgcacgct tgcaggaac aattaaaac tggccttga aaatacaag aaaaactca aacaacagg cggccgtgg tcaaggcaa aattaccgg taccgccga gtactaatc tacaaacgc cagcaagcg agcaaaaac aagtctatt cggctaaag tgaaacagc cggaagttg tacaaccgg aaacgaaaa aagaaccga cgccgtttg aaacggctc tacaccaag ccgctgcga taacgtacg acccgttct acagcccca ttttagaga aaattgata agatcttgg tcggcttgg gcttcaacg aggagccgt gcagggaaa ggctcattt gtagcatgt acaaaaatc cggtcaagt tcagtccgc acagtaatc tcctatcag cggaattaa gcagagatt ccaaagaac tgcaaaagc ccacaacaa ccaacgctg ccgtgatcc gattctatc gctacgtgc aagcagcca gagacggcc aagacaaac gtgaatgcc taagacttt atccatcat gttccataa atacagaac aataagtgc agtgcagtg taaataaag ctcagtcat tttgtaaat aattagttg ctttaagaa atcattatg ttattgctc aatagtgac gcttttgta aaaatatta ataacaaaa gcatgagta aatctagtg tatgctgct tagtctaat ttctaagga aaactctgg ctagtcaga gtcggaatt gtcgagtac ctaaattgt tttttactt ttttaagcg ctagagact gtgaatggt tgatcgatc gattgatag taactttat ttatttatt tatgtaatt tatttattt aattattta ttgccaact gtaaatttg taaagttat ttataataa atacatgac tacagg
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
