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Gene information
General information
TC012802 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15107415..15110298
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012802
    iBeetle screen iB_05324

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    beetle stock: bad (increased lethality),
    Pupal morphology
    beetle stock: bad (increased lethality),
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    appendages sometimes remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    head partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    procephalic head partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012802 RA
      MADSKRKRK LEDAKSEAK KPKIESFEP QWSDHELVA 
      EQLGLGLNV AKNVVKLFE DGNTVPFIA RYRRDVTNN 
      LDAEQLRAV KDTCEEISA LRGKIGSVL KQLDKSGVL 
      NEQLRRSVV NSRSIEELE YIYAPYKSG SKRTLAERA 
      KEQGLEAPA LALLNNTKR VRFDDFVKI DDGLDIKAV 
      KNGIAHIIA AVISTDSEI LSSLREFRK KANIIIKTK 
      KAATKHKVE NSEKFDNYF DFEVPLKYI KPHQVLAIN 
      RGENLKILS VKVVLPDSL FNEFRKKCD KWTSLRGDR 
      VRQDVVKRA IEDSYQRLV KPLIKREIR AELKQNAER 
      ASCQVFSSN LKDLLLAPP LRGQTILGL DPGFTNGCK 
      LALISPLGD LIDQNIIYP HKRKNNDDV TIIRDMLLE 
      NNCELIALG NGTACRQTE EWLSDLIKS QVFSPLDVK 
      YTVVREEGA SIYSCSPEA KKEFPTIDP NIISAVSLA 
      RRLQDPLAE LVKIEPKHL GVGMYQHDI KKRQLEEAL 
      NDVVSECVS FVGVDLNTA SRCLLRHVA GLSDKKAQQ 
      IIDYRAENG PFISRQQLL KVSGIGQKI FTQCAGFVR 
      VVPPSGETM KYNKLDSTN IHPESYEMA TKLLKMFNL 
      RLCDIGTTG FITIAKREV PKLDLSKLS QMFGSNEQT 
      LRLILDAFC QPLNYDLRS DYDKKPVFK KGLIEISDL 
      KSGIVLTGV VTNVTHFGS FVDIGVGIN GLIHVSKSN 
      GLDLHLGNK VEVKVLSID VEKKRIGLQ ALKILN
    CDS sequence
    • >TC012802 RA
      atggcagat tcgaagcgc aaacggaag cttgaagat 
      gccaaaagt gaggcgaaa aagccgaaa atcgagtca 
      ttcgagccc caatggagc gaccacgaa ttggtggct 
      gaacagctc ggtttgggg ctaaatgtg gccaaaaac 
      gttgttaaa ttgttcgag gatggaaac accgttccg 
      tttatcgcg agatacaga cgcgatgtt actaataat 
      cttgacgcc gagcagttg cgtgctgtt aaagacact 
      tgtgaggag ataagtgcc cttagaggt aagattggc 
      agtgttttg aaacagttg gacaagtca ggggtgcta 
      aatgaacag ttgagacgc tctgtggtc aattcgagg 
      tcaattgag gagttggaa tacatttat gcgccctat 
      aaaagtggg agcaagcgg actttggcc gaaagggcc 
      aaagagcag gggttagag gcgcccgct ttggccctt 
      ttgaacaac acaaaacgc gtgagattt gacgatttt 
      gttaaaatt gacgatggg cttgatatc aaagctgtt 
      aagaacggg attgctcat attatcgcg gctgttatt 
      tcaaccgac agtgagatt ttatcgtcc ctaagagaa 
      tttcggaaa aaagccaac attattatt aagaccaag 
      aaagctgcg actaaacac aaagtcgaa aatagtgaa 
      aagtttgat aattatttc gatttcgag gttcccctt 
      aaatacatc aagccacac caagttttg gccattaat 
      aggggcgaa aatttgaaa attctttcg gttaaagtt 
      gtgcttccg gactcactt ttcaacgag tttcggaaa 
      aagtgtgac aaatggacg agcttaagg ggcgatcgt 
      gtgagacaa gacgtcgta aaacgggcc attgaggac 
      tcataccaa cggttagtt aaacctttg attaagcgc 
      gaaattcga gccgaactg aaacaaaac gccgagcga 
      gcatcttgt caagttttc agttcgaat ttaaaagat 
      ttgctcctg gcgccccct ttgaggggc caaaccatc 
      ctaggcctt gaccctggc ttcaccaat ggctgcaaa 
      ctagcccta atatcacca ctgggcgac ctcatcgac 
      caaaacata atttacccc cataaaaga aaaaataac 
      gacgatgta acaataatc agagacatg ttacttgaa 
      aacaactgt gaattaatt gccttgggt aacggaaca 
      gcgtgtcgt caaaccgaa gaatggctc tcagattta 
      atcaaaagc caggttttt agccccctt gacgtcaaa 
      tatacggtg gtgagggaa gaaggggct tcgatttat 
      tcctgcagc cctgaagcc aagaaggag ttcccgaca 
      attgacccc aatatcata agcgctgtg tctctagcc 
      cgacggttg caggacccc cttgccgag ctggtcaaa 
      atcgagcca aaacacctg ggggtaggt atgtaccaa 
      catgacatc aagaaaaga caactagag gaagcccta 
      aatgacgtt gtttcggaa tgtgtcagt ttcgtgggt 
      gtggacttg aacacggct tcgcgctgc cttttacga 
      catgtggcg ggcctgtct gacaaaaaa gcccaacaa 
      ataattgac tatcgtgca gaaaatggc ccctttatt 
      tcgcgccaa caattgttg aaagtgtcg gggattggg 
      cagaaaatt ttcacacag tgtgctggg tttgtacgt 
      gtggtgcca ccgagcggc gaaactatg aaatacaat 
      aaattagac agcacaaat attcatcca gaatcgtac 
      gaaatggcc acaaagttg cttaaaatg tttaattta 
      aggttgtgt gatattggc accactggt tttattact 
      atagctaag agggaagtc ccgaagttg gatctaagc 
      aagttgagt cagatgttt ggttccaac gaacaaacg 
      cttcgcttg attttggat gcgttttgc caaccgtta 
      aactatgat cttcgaagt gattatgat aaaaaacca 
      gtttttaag aaaggttta attgagatt agtgacctt 
      aaaagtggg attgttttg accggagtt gtgactaat 
      gtcactcat tttgggagt tttgtcgat ataggagtc 
      ggaattaac ggtttgatc cacgtcagt aagtccaac 
      ggtttggac ttacatctg gggaataaa gttgaagtt 
      aaagtttta agtattgac gttgagaag aagcggatt 
      ggcttacag gcgctcaaa attctcaat tga
    mRNA sequence
    • >TC012802 RA
      ggtgttaag tggaatagc attctagca ttacttcag 
      cttttctca ttttttcta ttattatta tcagagtta 
      ttaattatt tattattct tccaatgag ggcaaatag 
      ccgaaattg gattttgtg cactttcga ccacttatt 
      gattggggt tatgtgaat gtttacaat ggcagattc 
      gaagcgcaa acggaagct tgaagatgc caaaagtga 
      ggcgaaaaa gccgaaaat cgagtcatt cgagcccca 
      atggagcga ccacgaatt ggtggctga acagctcgg 
      tttggggct aaatgtggc caaaaacgt tgttaaatt 
      gttcgagga tggaaacac cgttccgtt tatcgcgag 
      atacagacg cgatgttac taataatct tgacgccga 
      gcagttgcg tgctgttaa agacacttg tgaggagat 
      aagtgccct tagaggtaa gattggcag tgttttgaa 
      acagttgga caagtcagg ggtgctaaa tgaacagtt 
      gagacgctc tgtggtcaa ttcgaggtc aattgagga 
      gttggaata catttatgc gccctataa aagtgggag 
      caagcggac tttggccga aagggccaa agagcaggg 
      gttagaggc gcccgcttt ggccctttt gaacaacac 
      aaaacgcgt gagatttga cgattttgt taaaattga 
      cgatgggct tgatatcaa agctgttaa gaacgggat 
      tgctcatat tatcgcggc tgttatttc aaccgacag 
      tgagatttt atcgtccct aagagaatt tcggaaaaa 
      agccaacat tattattaa gaccaagaa agctgcgac 
      taaacacaa agtcgaaaa tagtgaaaa gtttgataa 
      ttatttcga tttcgaggt tccccttaa atacatcaa 
      gccacacca agttttggc cattaatag gggcgaaaa 
      tttgaaaat tctttcggt taaagttgt gcttccgga 
      ctcactttt caacgagtt tcggaaaaa gtgtgacaa 
      atggacgag cttaagggg cgatcgtgt gagacaaga 
      cgtcgtaaa acgggccat tgaggactc ataccaacg 
      gttagttaa acctttgat taagcgcga aattcgagc 
      cgaactgaa acaaaacgc cgagcgagc atcttgtca 
      agttttcag ttcgaattt aaaagattt gctcctggc 
      gcccccttt gaggggcca aaccatcct aggccttga 
      ccctggctt caccaatgg ctgcaaact agccctaat 
      atcaccact gggcgacct catcgacca aaacataat 
      ttaccccca taaaagaaa aaataacga cgatgtaac 
      aataatcag agacatgtt acttgaaaa caactgtga 
      attaattgc cttgggtaa cggaacagc gtgtcgtca 
      aaccgaaga atggctctc agatttaat caaaagcca 
      ggtttttag cccccttga cgtcaaata tacggtggt 
      gagggaaga aggggcttc gatttattc ctgcagccc 
      tgaagccaa gaaggagtt cccgacaat tgaccccaa 
      tatcataag cgctgtgtc tctagcccg acggttgca 
      ggaccccct tgccgagct ggtcaaaat cgagccaaa 
      acacctggg ggtaggtat gtaccaaca tgacatcaa 
      gaaaagaca actagagga agccctaaa tgacgttgt 
      ttcggaatg tgtcagttt cgtgggtgt ggacttgaa 
      cacggcttc gcgctgcct tttacgaca tgtggcggg 
      cctgtctga caaaaaagc ccaacaaat aattgacta 
      tcgtgcaga aaatggccc ctttatttc gcgccaaca 
      attgttgaa agtgtcggg gattgggca gaaaatttt 
      cacacagtg tgctgggtt tgtacgtgt ggtgccacc 
      gagcggcga aactatgaa atacaataa attagacag 
      cacaaatat tcatccaga atcgtacga aatggccac 
      aaagttgct taaaatgtt taatttaag gttgtgtga 
      tattggcac cactggttt tattactat agctaagag 
      ggaagtccc gaagttgga tctaagcaa gttgagtca 
      gatgtttgg ttccaacga acaaacgct tcgcttgat 
      tttggatgc gttttgcca accgttaaa ctatgatct 
      tcgaagtga ttatgataa aaaaccagt ttttaagaa 
      aggtttaat tgagattag tgaccttaa aagtgggat 
      tgttttgac cggagttgt gactaatgt cactcattt 
      tgggagttt tgtcgatat aggagtcgg aattaacgg 
      tttgatcca cgtcagtaa gtccaacgg tttggactt 
      acatctggg gaataaagt tgaagttaa agttttaag 
      tattgacgt tgagaagaa gcggattgg cttacaggc 
      gctcaaaat tctcaattg atttgagtt tggcgccat 
      tttttacgc aagccaact aaaaataca ttattttcc 
      gacat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY