| General information | |||
| TC034462
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 15047925..15091547 | |
| Sequence information | |||
| Transcripts / Proteins (3) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC034462 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05323 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC034462 RA
MSWSEPLPQ EPEPVDADL WPSSENVLN ETEDIKIRL NITLQHCTS LYTNRTTAL AETHPDGFC PVTTDGLLC WPPTPINET TYVKCFAEL MNIRYDDTQ NATRVCLAN GTWTKADYS KCTEIILIP DVETQATIY FVGYVLSLI TLSIALGIF TYFKELRCL RNRIHMNLM WSYMLMYIM WILTLTVLG SKGGTGASI ACIFVITLL HYFHISTFF WMFVEGLYL YILVVETLT RENYKLRVY VCIGWGLPM IFILVWVIV KSFIPAAGD PATCTWFNS HDVDWIFQG PTMLVLLLN LAFLLAIMW VLITKLRSA NTVETQQYH KAAKALLVL MPLLGITYV ITIYAPTPD KKSEIIFEC VRAVLLSTQ GFTVALFYC FLNTEVQNT VRHHFETWK TRRSLGPSR LRSGSRSKD WSPRSRTES IRNTEWTLV MPDDVDAEG KVSPPVNGS VR
- >TC034462 RB
MSWSEPLPQ EPEPVDADL WPSSENVLN ETEDIKIRL NITLQHCTS LYTNRTTAL AETHPDGFC PVTTDGLLC WPPTPINET TYVKCFAEL MNIRYDDTQ NATRVCLAN GTWTKADYS KCTEIILIP DVETQATIY FVGYVLSLI TLSIALGIF TYFKELRCL RNRIHMNLM WSYMLMYIM WILTLTVLG SKGGTGASI ACIFVITLL HYFHISTFF WMFVEGLYL YILVVETLT RENYKLRVY VCIGWGLPM IFILVWVIV KSFIPAAGD PATCTWFNS HDVDWIFQG PTMLVLLLN LAFLLAIMW VLITKLRSA NTVETQQYH KAAKALLVL MPLLGITYV ITIYAPTPD KKSEIIFEC VRAVLLSTQ GFTVALFYC FLNTEVQNT VRHHFETWK TRRSLGPSR LRSGSRSKD WSPRSRTES IRLYSQPTY HKRESCTSE VTITTLVTV NGGTRPSSN GSAQSRSPF LHPPRPSYG GGNV
- >TC034462 RC
MAKCIDLTC NHVRYRLYS QPTYHKRES CTSEVTITT LVTVNGGTR PSSNGSAQS RSPFLHPPR PSYGGGNV
- >TC034462 RA
atgagttgg tccgaacct ctcccccag gagccggag cccgtcgac gccgacttg tggcccagt tccgagaat gtcctcaac gaaacagag gacatcaag atccgactc aacatcacc ttacaacac tgcaccagc ctctatacc aacaggacc acagccctc gcagaaacc cacccagat gggttttgc cccgtcacc accgatggg ctgctgtgc tggccgccc acgcccatc aacgagacg acctatgtc aagtgcttc gccgaattg atgaacatc agatacgac gacacacag aatgccaca agagtttgc ctcgctaat gggacatgg acgaaagcc gattattcc aaatgcacc gaaatcatt cttatcccc gacgtcgaa acccaagcc acgatttat ttcgtcgga tacgtttta agtttaatt acactgtcg atagctttg ggaattttc acatatttc aaagagctg cgctgcttg agaaaccga attcacatg aatctcatg tggtcttac atgttaatg tacataatg tggattctg accctgacg gttctgggc tccaaaggg ggcactgga gcctccata gcttgcata ttcgtcatc acactgcta cattacttc cacatttcc acgtttttt tggatgttt gttgagggc ctgtatctt tatattctc gtcgttgaa acactgacc agggagaat tataaattg agggtttat gtgtgcatc gggtggggc ttgccaatg attttcatc ctcgtttgg gtgattgtc aagagcttt atcccggca gcgggcgac ccagccaca tgcacttgg ttcaacagt catgacgtg gactggatt ttccaaggg cctacaatg ctggttctc ctgctgaat ttggccttc ctgctggcc ataatgtgg gtcctcata acaaaactg cgatctgcc aatacggtg gaaacccaa cagtatcat aaagcggcc aaagccctc ctcgtactt atgcccttg ctgggcatc acctacgtt atcacaatc tacgcccca acccctgat aagaaatcg gaaatcatt ttcgagtgc gtccgcgct gtccttctc tcgacacag ggcttcact gtcgctcta ttctactgc ttcttaaac acggaggtg cagaacacc gtccgccac cacttcgaa acgtggaaa acacgacga tctctgggc ccatccaga ctacgttcg gggagtcgc agcaaggac tggtccccc aggtcacgc acggagagt atacgaaac acggaatgg acgttggta atgccggac gatgtcgat gccgaagga aaagtctct cctcccgtc aacggaagc gtccgttaa
- >TC034462 RB
atgagttgg tccgaacct ctcccccag gagccggag cccgtcgac gccgacttg tggcccagt tccgagaat gtcctcaac gaaacagag gacatcaag atccgactc aacatcacc ttacaacac tgcaccagc ctctatacc aacaggacc acagccctc gcagaaacc cacccagat gggttttgc cccgtcacc accgatggg ctgctgtgc tggccgccc acgcccatc aacgagacg acctatgtc aagtgcttc gccgaattg atgaacatc agatacgac gacacacag aatgccaca agagtttgc ctcgctaat gggacatgg acgaaagcc gattattcc aaatgcacc gaaatcatt cttatcccc gacgtcgaa acccaagcc acgatttat ttcgtcgga tacgtttta agtttaatt acactgtcg atagctttg ggaattttc acatatttc aaagagctg cgctgcttg agaaaccga attcacatg aatctcatg tggtcttac atgttaatg tacataatg tggattctg accctgacg gttctgggc tccaaaggg ggcactgga gcctccata gcttgcata ttcgtcatc acactgcta cattacttc cacatttcc acgtttttt tggatgttt gttgagggc ctgtatctt tatattctc gtcgttgaa acactgacc agggagaat tataaattg agggtttat gtgtgcatc gggtggggc ttgccaatg attttcatc ctcgtttgg gtgattgtc aagagcttt atcccggca gcgggcgac ccagccaca tgcacttgg ttcaacagt catgacgtg gactggatt ttccaaggg cctacaatg ctggttctc ctgctgaat ttggccttc ctgctggcc ataatgtgg gtcctcata acaaaactg cgatctgcc aatacggtg gaaacccaa cagtatcat aaagcggcc aaagccctc ctcgtactt atgcccttg ctgggcatc acctacgtt atcacaatc tacgcccca acccctgat aagaaatcg gaaatcatt ttcgagtgc gtccgcgct gtccttctc tcgacacag ggcttcact gtcgctcta ttctactgc ttcttaaac acggaggtg cagaacacc gtccgccac cacttcgaa acgtggaaa acacgacga tctctgggc ccatccaga ctacgttcg gggagtcgc agcaaggac tggtccccc aggtcacgc acggagagt atacgactg tacagtcag ccgacgtac cacaaaagg gagtcgtgc acttccgaa gtgaccatc acgacgttg gtcaccgtc aacgggggc accaggccc tcctccaac ggcagcgcg cagagcagg agccccttc ctgcaccct ccaagaccc agctatggc ggaggcaac gtttag
- >TC034462 RC
atggcaaag tgtattgat ttgacctgc aatcatgtt cgatacaga ctgtacagt cagccgacg taccacaaa agggagtcg tgcacttcc gaagtgacc atcacgacg ttggtcacc gtcaacggg ggcaccagg ccctcctcc aacggcagc gcgcagagc aggagcccc ttcctgcac cctccaaga cccagctat ggcggaggc aacgtttag
- >TC034462 RA
actttgtta atttgctcg ctggtggct cgagggatt aaataatgc gacagtgga aacggggaa ttgggatgg aacgaacac gtgatcttt gtttggacg agacgtccc ggaattgat agcaagatt cgagtgtca aaatgcatc atcaacaat gagttggtc cgaacctct cccccagga gccggagcc cgtcgacgc cgacttgtg gcccagttc cgagaatgt cctcaacga aacagagga catcaagat ccgactcaa catcacctt acaacactg caccagcct ctataccaa caggaccac agccctcgc agaaaccca cccagatgg gttttgccc cgtcaccac cgatgggct gctgtgctg gccgcccac gcccatcaa cgagacgac ctatgtcaa gtgcttcgc cgaattgat gaacatcag atacgacga cacacagaa tgccacaag agtttgcct cgctaatgg gacatggac gaaagccga ttattccaa atgcaccga aatcattct tatccccga cgtcgaaac ccaagccac gatttattt cgtcggata cgttttaag tttaattac actgtcgat agctttggg aattttcac atatttcaa agagctgcg ctgcttgag aaaccgaat tcacatgaa tctcatgtg gtcttacat gttaatgta cataatgtg gattctgac cctgacggt tctgggctc caaaggggg cactggagc ctccatagc ttgcatatt cgtcatcac actgctaca ttacttcca catttccac gtttttttg gatgtttgt tgagggcct gtatcttta tattctcgt cgttgaaac actgaccag ggagaatta taaattgag ggtttatgt gtgcatcgg gtggggctt gccaatgat tttcatcct cgtttgggt gattgtcaa gagctttat cccggcagc gggcgaccc agccacatg cacttggtt caacagtca tgacgtgga ctggatttt ccaagggcc tacaatgct ggttctcct gctgaattt ggccttcct gctggccat aatgtgggt cctcataac aaaactgcg atctgccaa tacggtgga aacccaaca gtatcataa agcggccaa agccctcct cgtacttat gcccttgct gggcatcac ctacgttat cacaatcta cgccccaac ccctgataa gaaatcgga aatcatttt cgagtgcgt ccgcgctgt ccttctctc gacacaggg cttcactgt cgctctatt ctactgctt cttaaacac ggaggtgca gaacaccgt ccgccacca cttcgaaac gtggaaaac acgacgatc tctgggccc atccagact acgttcggg gagtcgcag caaggactg gtcccccag gtcacgcac ggagagtat acgaaacac ggaatggac gttggtaat gccggacga tgtcgatgc cgaaggaaa agtctctcc tcccgtcaa cggaagcgt ccgttaaga tcgtccatc ccgagggct ttgcacgga aaacaattt cataataat aataacggg acgcttctt ttggagttc cttattgcc gctggtcta acgtgtgct ttggatggg agccgggat tgtgttcaa atgtttcat attctctca aattgaatt ggaaaaaca ttgcgtaaa cgttgattt ttacatgtt tttgtgttg tgtattggc aagggagac gacgtcggc gagtctcac aggaaaaca gtggaaaaa ataataatt tcttcgatt catgacaaa atatggaac atgttgaat gcgtttgtt tcatttatt ttcttcttt ctctttaaa ttaaccaaa ggccaagct aaactatgt atttatatt ccggggagg aggtccaaa agtcggggt ttgatttat ggaccattt taaacactc atacaaagg cgaaattta ttttaactg gttaaatat ttattcgga atgattgcg tctgtaata acacgtaat gttggcact tgggcacga cgcaaatat ttattttca tcaataaaa aatagtgaa ttatttagt ttacttttc ctaaactca ccctgtatg aaaaaatgt tttgttttg taaattaac cacactcct tagaaacaa atttaatac aaaaataaa gaactaatt aattttgcg atctttgat gttgcaaac acgccgttt aattttttg cccgacgac gaaactgaa caatattta tttacccaa agtttcgcc tacgacttg gtgaatatt taactcgtc tccgacatg agtagagag aactcgtgg aatcaataa ttaaattgc aagtttcct cgtcatact gtttgaaaa atctgcttt tcccatgca acattaata tctttctcc ggcctttgg cagccccgg cccacattc ctattaatg tacctgtat tatcatcac tgtcactgc atttaataa acttttaat ttgtca
- >TC034462 RB
actttgtta atttgctcg ctggtggct cgagggatt aaataatgc gacagtgga aacggggaa ttgggatgg aacgaacac gtgatcttt gtttggacg agacgtccc ggaattgat agcaagatt cgagtgtca aaatgcatc atcaacaat gagttggtc cgaacctct cccccagga gccggagcc cgtcgacgc cgacttgtg gcccagttc cgagaatgt cctcaacga aacagagga catcaagat ccgactcaa catcacctt acaacactg caccagcct ctataccaa caggaccac agccctcgc agaaaccca cccagatgg gttttgccc cgtcaccac cgatgggct gctgtgctg gccgcccac gcccatcaa cgagacgac ctatgtcaa gtgcttcgc cgaattgat gaacatcag atacgacga cacacagaa tgccacaag agtttgcct cgctaatgg gacatggac gaaagccga ttattccaa atgcaccga aatcattct tatccccga cgtcgaaac ccaagccac gatttattt cgtcggata cgttttaag tttaattac actgtcgat agctttggg aattttcac atatttcaa agagctgcg ctgcttgag aaaccgaat tcacatgaa tctcatgtg gtcttacat gttaatgta cataatgtg gattctgac cctgacggt tctgggctc caaaggggg cactggagc ctccatagc ttgcatatt cgtcatcac actgctaca ttacttcca catttccac gtttttttg gatgtttgt tgagggcct gtatcttta tattctcgt cgttgaaac actgaccag ggagaatta taaattgag ggtttatgt gtgcatcgg gtggggctt gccaatgat tttcatcct cgtttgggt gattgtcaa gagctttat cccggcagc gggcgaccc agccacatg cacttggtt caacagtca tgacgtgga ctggatttt ccaagggcc tacaatgct ggttctcct gctgaattt ggccttcct gctggccat aatgtgggt cctcataac aaaactgcg atctgccaa tacggtgga aacccaaca gtatcataa agcggccaa agccctcct cgtacttat gcccttgct gggcatcac ctacgttat cacaatcta cgccccaac ccctgataa gaaatcgga aatcatttt cgagtgcgt ccgcgctgt ccttctctc gacacaggg cttcactgt cgctctatt ctactgctt cttaaacac ggaggtgca gaacaccgt ccgccacca cttcgaaac gtggaaaac acgacgatc tctgggccc atccagact acgttcggg gagtcgcag caaggactg gtcccccag gtcacgcac ggagagtat acgactgta cagtcagcc gacgtacca caaaaggga gtcgtgcac ttccgaagt gaccatcac gacgttggt caccgtcaa cgggggcac caggccctc ctccaacgg cagcgcgca gagcaggag ccccttcct gcaccctcc aagacccag ctatggcgg aggcaacgt ttaggctgt ttttttgtg attttttca aagacttat tactgagtt gtgaacagg gtcgctgta gcactttta ttctaatta tattaaata tttttgttc ggt
- >TC034462 RC
gttctttgc atggcaaag tgtattgat ttgacctgc aatcatgtt cgatacaga ctgtacagt cagccgacg taccacaaa agggagtcg tgcacttcc gaagtgacc atcacgacg ttggtcacc gtcaacggg ggcaccagg ccctcctcc aacggcagc gcgcagagc aggagcccc ttcctgcac cctccaaga cccagctat ggcggaggc aacgtttag gctgttttt ttgtgattt tttcaaaga cttattact gagttgtga acagggtcg ctgtagcac ttttattct aattatatt aaatatttt tgttcggt
