Status
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Gene information
General information
TC012797 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15034789..15038943
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012797
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05322

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Pupal morphology
    beetle stock: bad (increased lethality),
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012797 RA
      MLDHSGYKI GVVIAVSVL VFIPILYYN NDFLDRRTI 
      FFTTESDEV YNESKYLVA TPKCKIPNL DPFEAEILK 
      FYKPMKYIS CSKRDFLTH VTKNDNIAT IHINTNAVP 
      SQLREHISC CMASITRSK NKEKPDDSI RISACKDFT 
      DNTTLTHDF IMVKCIDLK TKKQIYENT HAAITQTKQ 
      IKAKITSMN TSATSPFSV LFVGIDSIS RLNFIRALP 
      QTYQYLEEN AWIPLRGYN KMGDNTFPN VMAIMTGFN 
      ESKTYRVCN PSSLGQMDK CPMMWFDYQ KLNYVTGYA 
      EDEAPISTF NYKKKGFTK PPTDYYFRP YMLGSERLK 
      AVRRDGMAY CTGPETAGE RVLNIAKDF SESFKDVAS 
      FSFFWTNTF SHNDLNLPS GMDRKIRDF LEDIAAKGV 
      LDNTIIFFL SDHGIRFGE IRYTHTGWL EERLPFIYI 
      SVPDRFKRQ FPSEFRNLE TKRDHLTTP YDIYMTLQH 
      ILVLSGLNY TQKPSDGCP KCASLFAQT ESERSCEDA 
      AIEPHWCTC GGYNPVPKT NQTVQNAAK FVVGQIEER 
      IRARNGASK CAKFSVGRI ITARVSERF SYKSDVYFL 
      VVMETRPRA VFEATVLWK GEAQFELTG GISRLDKYA 
      EHSRCVEEA YLKMFCYCR
    CDS sequence
    • >TC012797 RA
      atgctcgac cacagcggc tacaaaatc ggggttgtt 
      attgcagtg tctgtgctc gttttcatc ccgattttg 
      tactacaat aacgacttt ctcgaccga agaaccatt 
      tttttcacc acagaatcc gacgaggtc tacaatgag 
      agtaaatat ttggtcgca acacccaaa tgcaaaatc 
      ccgaatctg gatccgttc gaggccgaa attttgaag 
      ttttacaag ccgatgaag tacatttct tgcagcaaa 
      cgcgatttt ttaactcat gtgacgaaa aacgacaat 
      attgccact atacatatt aacactaac gcagttccg 
      tcccagctt agagaacac atatcgtgc tgtatggcc 
      agtatcaca agaagcaag aacaaggag aagccggac 
      gacagcatc agaatatcg gcttgtaaa gatttcacg 
      gacaacacc accctaacc cacgacttc atcatggtg 
      aaatgcatc gacttgaaa acaaagaag caaatctac 
      gagaacacc cacgcggca atcacccaa acaaagcaa 
      atcaaagcc aaaatcacg tcaatgaac acaagtgca 
      acgagtcca ttcagtgtt ttattcgtc ggaatcgac 
      agcatatca cgcttaaat tttatacga gctttacca 
      caaacgtat caatacctc gaagagaac gcgtggatt 
      cccttacga ggttacaac aaaatgggc gataacacg 
      ttcccgaac gtcatggcg atcatgacc ggcttcaac 
      gagagcaaa acctacagg gtgtgcaac cctagcagc 
      ttgggccag atggacaag tgtcccatg atgtggttc 
      gactaccag aagttgaac tatgtgact ggatatgca 
      gaggacgag gctccgatc agcactttt aattacaag 
      aagaaaggg tttacgaaa ccaccgact gattattat 
      ttccggccc tatatgcta ggttccgag agactcaaa 
      gcggtacga agggacgga atggcttac tgtaccggc 
      cctgaaaca gccggtgaa cgcgtcttg aacatagct 
      aaagacttt tctgaaagt ttcaaagac gtggcaagt 
      ttctccttc ttctggacg aacactttc agtcataac 
      gacctcaac ctgccctca ggaatggac agaaaaatc 
      cgcgacttc ctggaggac atcgccgcc aaaggcgtg 
      ctagacaac accatcatc ttcttcctg agcgaccac 
      gggatcagg ttcggggaa atacggtac acgcacacc 
      gggtggttg gaggaacgc ctcccgttt atctacata 
      tcggttccc gaccgcttc aagcgccaa ttcccgtcc 
      gagttccga aacctggag acgaagcgc gaccacttg 
      accactccc tacgacatc tacatgacc ctgcaacat 
      atcctggtg ctctcagga ctcaactac acgcagaaa 
      ccgagcgat gggtgtccg aaatgcgcc tcacttttc 
      gcacaaacc gagtcggag agatcgtgc gaagacgca 
      gccatcgag ccgcattgg tgcacgtgt ggcggttat 
      aacccggtg ccaaaaacc aaccaaacc gttcaaaac 
      gctgccaaa ttcgttgtc ggccaaatt gaggagaga 
      atccgcgcc agaaatggg gcttcaaag tgtgccaaa 
      ttttccgta gggaggatt attacggcg agggtttcg 
      gaacggttt agttacaag agtgatgtt tattttctg 
      gtggtgatg gagacgagg cccagggcg gttttcgaa 
      gcgacggtg ctgtggaaa ggcgaggcg cagtttgag 
      ctgactgga gggataagt cgcctggat aagtacgct 
      gagcacagt aggtgtgtg gaggaggcc tatctgaag 
      atgttctgt tactgtcgg tag
    mRNA sequence
    • >TC012797 RA
      ttatagtga aacaaggtc tttccaatt ccgataaat 
      tcgccaccc actgattta tcaagtcgc gatctacgc 
      aaactcggg tatcattga tgttttctc aaatcatcc 
      cttttccca gtgccatgc tccacgtcc cgcactgaa 
      tacaagtgc tcaattacc cggaaaaat aatgacgcc 
      tccaacacc ttgaagttg cagcgaccg aaaaattag 
      caaggatat tatgacaaa atcggtcca aaatgctcg 
      accacagcg gctacaaaa tcggggttg ttattgcag 
      tgtctgtgc tcgttttca tcccgattt tgtactaca 
      ataacgact ttctcgacc gaagaacca tttttttca 
      ccacagaat ccgacgagg tctacaatg agagtaaat 
      atttggtcg caacaccca aatgcaaaa tcccgaatc 
      tggatccgt tcgaggccg aaattttga agttttaca 
      agccgatga agtacattt cttgcagca aacgcgatt 
      ttttaactc atgtgacga aaaacgaca atattgcca 
      ctatacata ttaacacta acgcagttc cgtcccagc 
      ttagagaac acatatcgt gctgtatgg ccagtatca 
      caagaagca agaacaagg agaagccgg acgacagca 
      tcagaatat cggcttgta aagatttca cggacaaca 
      ccaccctaa cccacgact tcatcatgg tgaaatgca 
      tcgacttga aaacaaaga agcaaatct acgagaaca 
      cccacgcgg caatcaccc aaacaaagc aaatcaaag 
      ccaaaatca cgtcaatga acacaagtg caacgagtc 
      cattcagtg ttttattcg tcggaatcg acagcatat 
      cacgcttaa attttatac gagctttac cacaaacgt 
      atcaatacc tcgaagaga acgcgtgga ttcccttac 
      gaggttaca acaaaatgg gcgataaca cgttcccga 
      acgtcatgg cgatcatga ccggcttca acgagagca 
      aaacctaca gggtgtgca accctagca gcttgggcc 
      agatggaca agtgtccca tgatgtggt tcgactacc 
      agaagttga actatgtga ctggatatg cagaggacg 
      aggctccga tcagcactt ttaattaca agaagaaag 
      ggtttacga aaccaccga ctgattatt atttccggc 
      cctatatgc taggttccg agagactca aagcggtac 
      gaagggacg gaatggctt actgtaccg gccctgaaa 
      cagccggtg aacgcgtct tgaacatag ctaaagact 
      tttctgaaa gtttcaaag acgtggcaa gtttctcct 
      tcttctgga cgaacactt tcagtcata acgacctca 
      acctgccct caggaatgg acagaaaaa tccgcgact 
      tcctggagg acatcgccg ccaaaggcg tgctagaca 
      acaccatca tcttcttcc tgagcgacc acgggatca 
      ggttcgggg aaatacggt acacgcaca ccgggtggt 
      tggaggaac gcctcccgt ttatctaca tatcggttc 
      ccgaccgct tcaagcgcc aattcccgt ccgagttcc 
      gaaacctgg agacgaagc gcgaccact tgaccactc 
      cctacgaca tctacatga ccctgcaac atatcctgg 
      tgctctcag gactcaact acacgcaga aaccgagcg 
      atgggtgtc cgaaatgcg cctcacttt tcgcacaaa 
      ccgagtcgg agagatcgt gcgaagacg cagccatcg 
      agccgcatt ggtgcacgt gtggcggtt ataacccgg 
      tgccaaaaa ccaaccaaa ccgttcaaa acgctgcca 
      aattcgttg tcggccaaa ttgaggaga gaatccgcg 
      ccagaaatg gggcttcaa agtgtgcca aattttccg 
      tagggagga ttattacgg cgagggttt cggaacggt 
      ttagttaca agagtgatg tttattttc tggtggtga 
      tggagacga ggcccaggg cggttttcg aagcgacgg 
      tgctgtgga aaggcgagg cgcagtttg agctgactg 
      gagggataa gtcgcctgg ataagtacg ctgagcaca 
      gtaggtgtg tggaggagg cctatctga agatgttct 
      gttactgtc ggtagtggt caagtgcct atttttcaa 
      cggtgccaa agtttgctc agacgggcg cgagtggag 
      aaatcaaaa aattgtgat actttgcat ttggtacaa 
      taaggattt tctagtcaa tgcgttatt gtaatcgat 
      gtcgttatt ttaggaggg ggattttat atcagttta 
      gtcttttgt gtcaataaa taagcactt ttatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY