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Gene information
General information
TC034460
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC012794
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15004209..15015123
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034460
    iBeetle screen iB_05320

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034460 RA
      MDRCRLIFS LFLMMYSSC SGHVLGDEC NSDWECKEH 
      ISGSICHRG RCLCQPFFA RLNQTACVQ STLLGYDCV 
      VPEQCSLKV ANSSCLDGT CRCVDGFLQ FRKHTCLGP 
      ARPGNVCYS NAHCRLWTA DSHCDFLIP NLFGRCQCN 
      SPFRQVGDS CVRSAFQTK PPTTTTTES IDFNLIGKN 
      EPPTTIKTT TKTTTTPKP TTTVPPATT GIRTRVDDA 
      AEAVSLGLP CVTDLQCRA ADPSSRCLD GVCDCAVRS 
      NTTGCSART RGCPPGTFQ CRSTGTCIS WFFVCDGRK 
      DCPDGSDEM CRGPQCPQE AFKCKTKCI SRAGRCDGV 
      KDCPDGEDE EDCQATRKR RCPPHTFQC GDGKCLPEY 
      EFCNAIIGC SDGSDEPAH ICRGRARRR LSEYCPLRC 
      GNGRCRSSA IACSGRDGC GDGTDERNC AVCRCPIVQ 
      GRNL
    CDS sequence
    • >TC034460 RA
      atggaccgc tgcaggctc attttcagc ctctttctc 
      atgatgtac agttcctgc tcgggccac gtcctcggt 
      gacgaatgc aacagcgat tgggaatgc aaagagcac 
      atttccgga tcgatatgt caccgaggg aggtgtctt 
      tgtcagccc ttcttcgcc agactcaac caaacggcc 
      tgcgtccaa tcaactttg cttggatat gactgcgtg 
      gtccctgaa cagtgctcc ctcaaggtc gccaatagc 
      agttgcttg gacgggacg tgccgttgc gtcgacgga 
      ttcctgcag ttccggaag cacacttgt ctaggacct 
      gcccgtccc ggaaacgtc tgctacagc aacgcccac 
      tgccgcctc tggaccgcc gacagccac tgcgacttc 
      ctcatcccc aacttattc ggtcgctgc caatgcaac 
      tctcccttc cgccaagtt ggcgactcc tgcgtccga 
      tccgcattc cagaccaaa cctccaacc acaaccacg 
      accgaatcc atcgatttc aacctcatc ggcaaaaac 
      gaaccgcca acaaccatc aaaaccacc acgaagacc 
      accaccacg cccaaacca acaacaacc gtgccgccg 
      gccaccacc gggatacgc acccgcgtg gacgacgcc 
      gccgaagcc gtcagcttg gggcttccg tgcgtgacg 
      gacctccaa tgccgcgcc gccgacccc tcctccagg 
      tgcctggac ggggtgtgt gactgcgcc gtgcgcagc 
      aacaccacg ggctgctcc gccaggacc cggggctgc 
      cctcccggg acgttccag tgtcggagc accgggacc 
      tgcatctcg tggtttttt gtctgtgac ggacgcaaa 
      gactgtccg gacggctcc gacgagatg tgtcggggg 
      ccccagtgc ccccaggag gccttcaaa tgcaagacc 
      aagtgcata tcgcgcgcc ggacgctgt gatggggtt 
      aaagactgt ccggacggc gaagacgag gaggattgc 
      caggccacc aggaagcga cgctgtcct cctcacacg 
      ttccaatgc ggcgatggc aagtgtctc ccggagtac 
      gagttttgc aacgctata attgggtgt agtgacggc 
      agcgacgaa ccggcgcac atctgcaga ggacgggca 
      cggagacgg ctctcggag tattgtcct ctgcgatgc 
      ggaaacgga aggtgcagg tcgagtgcc atcgcatgt 
      tcagggaga gacggctgt ggagatggc acagatgag 
      aggaactgt gccgtttgt aggtgtccc atcgtgcaa 
      gggaggaac ttatag
    mRNA sequence
    • >TC034460 RA
      gcgcttctc cagttcaac accaaccga agaactagc 
      acctgctgc tctgcagga gtaattcga gtgccgtgt 
      gcgggcttt atttcagtg cccgaaatg gaccgctgc 
      aggctcatt ttcagcctc tttctcatg atgtacagt 
      tcctgctcg ggccacgtc ctcggtgac gaatgcaac 
      agcgattgg gaatgcaaa gagcacatt tccggatcg 
      atatgtcac cgagggagg tgtctttgt cagcccttc 
      ttcgccaga ctcaaccaa acggcctgc gtccaatca 
      actttgctt ggatatgac tgcgtggtc cctgaacag 
      tgctccctc aaggtcgcc aatagcagt tgcttggac 
      gggacgtgc cgttgcgtc gacggattc ctgcagttc 
      cggaagcac acttgtcta ggacctgcc cgtcccgga 
      aacgtctgc tacagcaac gcccactgc cgcctctgg 
      accgccgac agccactgc gacttcctc atccccaac 
      ttattcggt cgctgccaa tgcaactct cccttccgc 
      caagttggc gactcctgc gtccgatcc gcattccag 
      accaaacct ccaaccaca accacgacc gaatccatc 
      gatttcaac ctcatcggc aaaaacgaa ccgccaaca 
      accatcaaa accaccacg aagaccacc accacgccc 
      aaaccaaca acaaccgtg ccgccggcc accaccggg 
      atacgcacc cgcgtggac gacgccgcc gaagccgtc 
      agcttgggg cttccgtgc gtgacggac ctccaatgc 
      cgcgccgcc gacccctcc tccaggtgc ctggacggg 
      gtgtgtgac tgcgccgtg cgcagcaac accacgggc 
      tgctccgcc aggacccgg ggctgccct cccgggacg 
      ttccagtgt cggagcacc gggacctgc atctcgtgg 
      ttttttgtc tgtgacgga cgcaaagac tgtccggac 
      ggctccgac gagatgtgt cgggggccc cagtgcccc 
      caggaggcc ttcaaatgc aagaccaag tgcatatcg 
      cgcgccgga cgctgtgat ggggttaaa gactgtccg 
      gacggcgaa gacgaggag gattgccag gccaccagg 
      aagcgacgc tgtcctcct cacacgttc caatgcggc 
      gatggcaag tgtctcccg gagtacgag ttttgcaac 
      gctataatt gggtgtagt gacggcagc gacgaaccg 
      gcgcacatc tgcagagga cgggcacgg agacggctc 
      tcggagtat tgtcctctg cgatgcgga aacggaagg 
      tgcaggtcg agtgccatc gcatgttca gggagagac 
      ggctgtgga gatggcaca gatgagagg aactgtgcc 
      gtttgtagg tgtcccatc gtgcaaggg aggaactta 
      tagttacaa cacgtccag caacactct ctgtaaaat 
      gccaaaaac tttgtatat tatctcaac cagcgactt 
      gtagtgtag actagtggt agattctat cagttataa 
      caaaagtat tatttatgt aatttaagt taacgctca 
      aaatatatc tgttagaaa taaatattt atttacttg 
      taacagtta agtataata aaattttga caaaacc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY