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Gene information
General information
TC012786 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 14863439..14865661
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC012786
    iBeetle screen iB_05318

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    beetle stock: bad (increased lethality),
    Pupal morphology
    beetle stock: bad (increased lethality),
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants strongly fragmented (cuticle crumbs) - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva complete everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012786 RA
      MGTFEKLKE LDLSRDEVE RLGAALKKP EFRQLLADY 
VHEVQNPEN RKLYEAEIT QLERERGVA VTFINPSPG 
YVLKTSDGV RKCFINVCS CELIDRPTS APMDKDGAR 
GLQWRLPHS LTPPREDLD NKKARCEVY DVVFHPDVI 
HMAQKNHQF QEIVNKTAC DAVEANFEV TLDKKNLKF 
PKLGYKGAP QSAIIRKPI KDFVPREME ETERQFYDK 
IYENADQKP KKTNVKRNV KKCAEGNSN YTTPKYVIK 
HRSHVDVQD CVEDKFARL NLTIPRELI VEINLPLLK 
ASSDITLDV TEKTVQLTS EKPSKYKLN LTLPYRVNQ 
DSGSAKFDK DLKKLIITL PVKRCELPR DDSGVESDP 
SPHSEDDVI EAKTPSFSD FRTNSCDYE LPDFTCHLF 
DKTLAFTLN VRNVSQDSI EKQVTCDSL HLKFASLSS 
SFFESNYAF YVRIDSGLD PEKLSVETW DNNVVVQIP 
VNCDNLEYY YYGLSEHDL RKKYIEEPS VLNSVLQSP 
IPEEPEPEP EESVTPAIN IANSYESSG DELSCSFSP 
RKGRGILKR LPNRSVSES SLDDMVASS IDDGEMSTS 
LKKTVRFND VIMRQLYRS NSSILGQKK KNQRKARNK 
KRAQERRHS ESEASEKED EKREEKSDY IHSDIFDMD 
LGV
    CDS sequence
    • >TC012786 RA
      atggggact tttgagaaa ctcaaagag ctggacctc 
tcccgcgac gaagtggag cgtctgggg gccgccctc 
aagaaaccc gagttccgc caactcctc gctgactac 
gtgcacgag gtgcaaaac cccgaaaac cggaagctg 
tacgaagcg gaaatcacg caactggag cgcgagcgc 
ggcgtcgcc gtcaccttc atcaacccc agccccggc 
tacgtccta aagaccagc gatggggtc cgcaagtgc 
ttcatcaac gtctgctcc tgcgaactc atcgacagg 
cccacctcg gcccccatg gacaaggac ggggcgcgc 
ggcctccag tggcgcctg ccccactcg ctgacgccc 
ccacgggag gacttggac aacaagaag gcccggtgt 
gaggtctat gatgtggtg ttccacccc gatgtgata 
cacatggcc cagaaaaac caccagttt caggaaatt 
gtcaacaag acggcgtgt gacgccgtc gaggcgaat 
ttcgaggtt acgttggac aagaagaat cttaagttc 
cccaagttg gggtacaag ggggcccct cagagcgcc 
ataatccgg aagccaatc aaggatttt gtcccgagg 
gagatggaa gagactgag cgacaattc tacgataaa 
atctatgaa aacgccgat caaaaaccg aaaaaaaca 
aatgtgaaa cgaaacgtt aagaagtgt gccgaaggc 
aactcgaat tacacgacc cccaaatat gtgataaaa 
caccggagt cacgtcgac gtgcaggac tgcgtcgag 
gacaaattc gcaagactc aacctgacg atccctcgg 
gaactcatc gttgagatc aacttgccc ttgctcaaa 
gcctcctcc gacatcacg ctcgacgtg acggaaaaa 
acagtacaa ctaaccagt gagaagcct tccaagtac 
aaactcaac ctgaccctc ccctacagg gtgaaccaa 
gacagcggt tcggccaag ttcgacaaa gacctgaag 
aagctcata atcacgttg ccggtgaaa cggtgcgag 
ttgccgcgc gacgacagc ggcgtcgag agcgacccc 
agcccccac tccgaggac gacgtgatc gaggcgaag 
acgcccagt tttagtgac tttcggact aattcgtgc 
gactacgaa ctgcccgac ttcacctgt cacctcttc 
gacaaaacc ctggcgttt accctaaac gtccgaaac 
gtgtcgcaa gactcgatc gaaaagcaa gtcacgtgc 
gactcgctc cacttgaag tttgccagt ctcagctcg 
agttttttc gagtcgaat tacgcgttt tacgtccgg 
attgactcc gggctcgac ccggagaaa ctctccgtt 
gagacgtgg gataataac gttgtggtc caaatcccg 
gtcaattgt gataacctt gagtactat tattacggc 
ctaagtgag cacgacttg cgcaagaag tatatcgag 
gagccctcg gtgcttaac agcgttttg caaagcccg 
atcccggag gagccggag ccggaaccg gaggagtcg 
gtgacgccg gcgattaat attgcgaat tcgtacgaa 
tcgagcggc gatgaattg agttgtagt tttagtccc 
aggaagggc cggggcatt ttgaagcgt ttgcccaat 
agaagtgtg tcggagtcg agtctggac gatatggtg 
gcgtcgtcg atcgatgat ggggaaatg tcgaccagt 
ttgaagaag acagtgcgg tttaatgat gtgataatg 
aggcaattg tatcgctcc aattcgagt attttgggg 
cagaagaag aagaatcag cggaaggcg aggaacaag 
aagagggcg caagagagg agacatagt gagagtgaa 
gcgtcggag aaggaggat gagaagagg gaggaaaag 
agtgattat attcatagt gatattttc gatatggat 
ttgggggtg tag
    mRNA sequence
    • >TC012786 RA
      ccccaactg tcacagttc aaatctccc gctactatc 
gatctgaca cccggccgg ctgattcgt ccacgtgaa 
cactcggaa tattccaga cgcagtttg tttattttg 
gtttcggtg cgcacaaat ttcctcaaa aacctgcat 
caaaaccct caaaaatgg ggacttttg agaaactca 
aagagctgg acctctccc gcgacgaag tggagcgtc 
tgggggccg ccctcaaga aacccgagt tccgccaac 
tcctcgctg actacgtgc acgaggtgc aaaaccccg 
aaaaccgga agctgtacg aagcggaaa tcacgcaac 
tggagcgcg agcgcggcg tcgccgtca ccttcatca 
accccagcc ccggctacg tcctaaaga ccagcgatg 
gggtccgca agtgcttca tcaacgtct gctcctgcg 
aactcatcg acaggccca cctcggccc ccatggaca 
aggacgggg cgcgcggcc tccagtggc gcctgcccc 
actcgctga cgcccccac gggaggact tggacaaca 
agaaggccc ggtgtgagg tctatgatg tggtgttcc 
accccgatg tgatacaca tggcccaga aaaaccacc 
agtttcagg aaattgtca acaagacgg cgtgtgacg 
ccgtcgagg cgaatttcg aggttacgt tggacaaga 
agaatctta agttcccca agttggggt acaaggggg 
cccctcaga gcgccataa tccggaagc caatcaagg 
attttgtcc cgagggaga tggaagaga ctgagcgac 
aattctacg ataaaatct atgaaaacg ccgatcaaa 
aaccgaaaa aaacaaatg tgaaacgaa acgttaaga 
agtgtgccg aaggcaact cgaattaca cgaccccca 
aatatgtga taaaacacc ggagtcacg tcgacgtgc 
aggactgcg tcgaggaca aattcgcaa gactcaacc 
tgacgatcc ctcgggaac tcatcgttg agatcaact 
tgcccttgc tcaaagcct cctccgaca tcacgctcg 
acgtgacgg aaaaaacag tacaactaa ccagtgaga 
agccttcca agtacaaac tcaacctga ccctcccct 
acagggtga accaagaca gcggttcgg ccaagttcg 
acaaagacc tgaagaagc tcataatca cgttgccgg 
tgaaacggt gcgagttgc cgcgcgacg acagcggcg 
tcgagagcg accccagcc cccactccg aggacgacg 
tgatcgagg cgaagacgc ccagtttta gtgactttc 
ggactaatt cgtgcgact acgaactgc ccgacttca 
cctgtcacc tcttcgaca aaaccctgg cgtttaccc 
taaacgtcc gaaacgtgt cgcaagact cgatcgaaa 
agcaagtca cgtgcgact cgctccact tgaagtttg 
ccagtctca gctcgagtt ttttcgagt cgaattacg 
cgttttacg tccggattg actccgggc tcgacccgg 
agaaactct ccgttgaga cgtgggata ataacgttg 
tggtccaaa tcccggtca attgtgata accttgagt 
actattatt acggcctaa gtgagcacg acttgcgca 
agaagtata tcgaggagc cctcggtgc ttaacagcg 
ttttgcaaa gcccgatcc cggaggagc cggagccgg 
aaccggagg agtcggtga cgccggcga ttaatattg 
cgaattcgt acgaatcga gcggcgatg aattgagtt 
gtagtttta gtcccagga agggccggg gcattttga 
agcgtttgc ccaatagaa gtgtgtcgg agtcgagtc 
tggacgata tggtggcgt cgtcgatcg atgatgggg 
aaatgtcga ccagtttga agaagacag tgcggttta 
atgatgtga taatgaggc aattgtatc gctccaatt 
cgagtattt tggggcaga agaagaaga atcagcgga 
aggcgagga acaagaaga gggcgcaag agaggagac 
atagtgaga gtgaagcgt cggagaagg aggatgaga 
agagggagg aaaagagtg attatattc atagtgata 
ttttcgata tggatttgg gggtgtaga tgtgatatt 
ttgaggttt tattatgtt cccagtgaa gtcatttgt 
actttttac atgttgctt tagaattgt agcgtatga 
tttattaaa taaaaattt acgagaaat
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