Status
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Gene information
General information
TC012786 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 14863439..14865661
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012786
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05318

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality),
    Pupal morphology
    beetle stock: bad (increased lethality),
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants strongly fragmented (cuticle crumbs) - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva complete everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012786 RA
      MGTFEKLKE LDLSRDEVE RLGAALKKP EFRQLLADY 
      VHEVQNPEN RKLYEAEIT QLERERGVA VTFINPSPG 
      YVLKTSDGV RKCFINVCS CELIDRPTS APMDKDGAR 
      GLQWRLPHS LTPPREDLD NKKARCEVY DVVFHPDVI 
      HMAQKNHQF QEIVNKTAC DAVEANFEV TLDKKNLKF 
      PKLGYKGAP QSAIIRKPI KDFVPREME ETERQFYDK 
      IYENADQKP KKTNVKRNV KKCAEGNSN YTTPKYVIK 
      HRSHVDVQD CVEDKFARL NLTIPRELI VEINLPLLK 
      ASSDITLDV TEKTVQLTS EKPSKYKLN LTLPYRVNQ 
      DSGSAKFDK DLKKLIITL PVKRCELPR DDSGVESDP 
      SPHSEDDVI EAKTPSFSD FRTNSCDYE LPDFTCHLF 
      DKTLAFTLN VRNVSQDSI EKQVTCDSL HLKFASLSS 
      SFFESNYAF YVRIDSGLD PEKLSVETW DNNVVVQIP 
      VNCDNLEYY YYGLSEHDL RKKYIEEPS VLNSVLQSP 
      IPEEPEPEP EESVTPAIN IANSYESSG DELSCSFSP 
      RKGRGILKR LPNRSVSES SLDDMVASS IDDGEMSTS 
      LKKTVRFND VIMRQLYRS NSSILGQKK KNQRKARNK 
      KRAQERRHS ESEASEKED EKREEKSDY IHSDIFDMD 
      LGV
    CDS sequence
    • >TC012786 RA
      atggggact tttgagaaa ctcaaagag ctggacctc 
      tcccgcgac gaagtggag cgtctgggg gccgccctc 
      aagaaaccc gagttccgc caactcctc gctgactac 
      gtgcacgag gtgcaaaac cccgaaaac cggaagctg 
      tacgaagcg gaaatcacg caactggag cgcgagcgc 
      ggcgtcgcc gtcaccttc atcaacccc agccccggc 
      tacgtccta aagaccagc gatggggtc cgcaagtgc 
      ttcatcaac gtctgctcc tgcgaactc atcgacagg 
      cccacctcg gcccccatg gacaaggac ggggcgcgc 
      ggcctccag tggcgcctg ccccactcg ctgacgccc 
      ccacgggag gacttggac aacaagaag gcccggtgt 
      gaggtctat gatgtggtg ttccacccc gatgtgata 
      cacatggcc cagaaaaac caccagttt caggaaatt 
      gtcaacaag acggcgtgt gacgccgtc gaggcgaat 
      ttcgaggtt acgttggac aagaagaat cttaagttc 
      cccaagttg gggtacaag ggggcccct cagagcgcc 
      ataatccgg aagccaatc aaggatttt gtcccgagg 
      gagatggaa gagactgag cgacaattc tacgataaa 
      atctatgaa aacgccgat caaaaaccg aaaaaaaca 
      aatgtgaaa cgaaacgtt aagaagtgt gccgaaggc 
      aactcgaat tacacgacc cccaaatat gtgataaaa 
      caccggagt cacgtcgac gtgcaggac tgcgtcgag 
      gacaaattc gcaagactc aacctgacg atccctcgg 
      gaactcatc gttgagatc aacttgccc ttgctcaaa 
      gcctcctcc gacatcacg ctcgacgtg acggaaaaa 
      acagtacaa ctaaccagt gagaagcct tccaagtac 
      aaactcaac ctgaccctc ccctacagg gtgaaccaa 
      gacagcggt tcggccaag ttcgacaaa gacctgaag 
      aagctcata atcacgttg ccggtgaaa cggtgcgag 
      ttgccgcgc gacgacagc ggcgtcgag agcgacccc 
      agcccccac tccgaggac gacgtgatc gaggcgaag 
      acgcccagt tttagtgac tttcggact aattcgtgc 
      gactacgaa ctgcccgac ttcacctgt cacctcttc 
      gacaaaacc ctggcgttt accctaaac gtccgaaac 
      gtgtcgcaa gactcgatc gaaaagcaa gtcacgtgc 
      gactcgctc cacttgaag tttgccagt ctcagctcg 
      agttttttc gagtcgaat tacgcgttt tacgtccgg 
      attgactcc gggctcgac ccggagaaa ctctccgtt 
      gagacgtgg gataataac gttgtggtc caaatcccg 
      gtcaattgt gataacctt gagtactat tattacggc 
      ctaagtgag cacgacttg cgcaagaag tatatcgag 
      gagccctcg gtgcttaac agcgttttg caaagcccg 
      atcccggag gagccggag ccggaaccg gaggagtcg 
      gtgacgccg gcgattaat attgcgaat tcgtacgaa 
      tcgagcggc gatgaattg agttgtagt tttagtccc 
      aggaagggc cggggcatt ttgaagcgt ttgcccaat 
      agaagtgtg tcggagtcg agtctggac gatatggtg 
      gcgtcgtcg atcgatgat ggggaaatg tcgaccagt 
      ttgaagaag acagtgcgg tttaatgat gtgataatg 
      aggcaattg tatcgctcc aattcgagt attttgggg 
      cagaagaag aagaatcag cggaaggcg aggaacaag 
      aagagggcg caagagagg agacatagt gagagtgaa 
      gcgtcggag aaggaggat gagaagagg gaggaaaag 
      agtgattat attcatagt gatattttc gatatggat 
      ttgggggtg tag
    mRNA sequence
    • >TC012786 RA
      ccccaactg tcacagttc aaatctccc gctactatc 
      gatctgaca cccggccgg ctgattcgt ccacgtgaa 
      cactcggaa tattccaga cgcagtttg tttattttg 
      gtttcggtg cgcacaaat ttcctcaaa aacctgcat 
      caaaaccct caaaaatgg ggacttttg agaaactca 
      aagagctgg acctctccc gcgacgaag tggagcgtc 
      tgggggccg ccctcaaga aacccgagt tccgccaac 
      tcctcgctg actacgtgc acgaggtgc aaaaccccg 
      aaaaccgga agctgtacg aagcggaaa tcacgcaac 
      tggagcgcg agcgcggcg tcgccgtca ccttcatca 
      accccagcc ccggctacg tcctaaaga ccagcgatg 
      gggtccgca agtgcttca tcaacgtct gctcctgcg 
      aactcatcg acaggccca cctcggccc ccatggaca 
      aggacgggg cgcgcggcc tccagtggc gcctgcccc 
      actcgctga cgcccccac gggaggact tggacaaca 
      agaaggccc ggtgtgagg tctatgatg tggtgttcc 
      accccgatg tgatacaca tggcccaga aaaaccacc 
      agtttcagg aaattgtca acaagacgg cgtgtgacg 
      ccgtcgagg cgaatttcg aggttacgt tggacaaga 
      agaatctta agttcccca agttggggt acaaggggg 
      cccctcaga gcgccataa tccggaagc caatcaagg 
      attttgtcc cgagggaga tggaagaga ctgagcgac 
      aattctacg ataaaatct atgaaaacg ccgatcaaa 
      aaccgaaaa aaacaaatg tgaaacgaa acgttaaga 
      agtgtgccg aaggcaact cgaattaca cgaccccca 
      aatatgtga taaaacacc ggagtcacg tcgacgtgc 
      aggactgcg tcgaggaca aattcgcaa gactcaacc 
      tgacgatcc ctcgggaac tcatcgttg agatcaact 
      tgcccttgc tcaaagcct cctccgaca tcacgctcg 
      acgtgacgg aaaaaacag tacaactaa ccagtgaga 
      agccttcca agtacaaac tcaacctga ccctcccct 
      acagggtga accaagaca gcggttcgg ccaagttcg 
      acaaagacc tgaagaagc tcataatca cgttgccgg 
      tgaaacggt gcgagttgc cgcgcgacg acagcggcg 
      tcgagagcg accccagcc cccactccg aggacgacg 
      tgatcgagg cgaagacgc ccagtttta gtgactttc 
      ggactaatt cgtgcgact acgaactgc ccgacttca 
      cctgtcacc tcttcgaca aaaccctgg cgtttaccc 
      taaacgtcc gaaacgtgt cgcaagact cgatcgaaa 
      agcaagtca cgtgcgact cgctccact tgaagtttg 
      ccagtctca gctcgagtt ttttcgagt cgaattacg 
      cgttttacg tccggattg actccgggc tcgacccgg 
      agaaactct ccgttgaga cgtgggata ataacgttg 
      tggtccaaa tcccggtca attgtgata accttgagt 
      actattatt acggcctaa gtgagcacg acttgcgca 
      agaagtata tcgaggagc cctcggtgc ttaacagcg 
      ttttgcaaa gcccgatcc cggaggagc cggagccgg 
      aaccggagg agtcggtga cgccggcga ttaatattg 
      cgaattcgt acgaatcga gcggcgatg aattgagtt 
      gtagtttta gtcccagga agggccggg gcattttga 
      agcgtttgc ccaatagaa gtgtgtcgg agtcgagtc 
      tggacgata tggtggcgt cgtcgatcg atgatgggg 
      aaatgtcga ccagtttga agaagacag tgcggttta 
      atgatgtga taatgaggc aattgtatc gctccaatt 
      cgagtattt tggggcaga agaagaaga atcagcgga 
      aggcgagga acaagaaga gggcgcaag agaggagac 
      atagtgaga gtgaagcgt cggagaagg aggatgaga 
      agagggagg aaaagagtg attatattc atagtgata 
      ttttcgata tggatttgg gggtgtaga tgtgatatt 
      ttgaggttt tattatgtt cccagtgaa gtcatttgt 
      actttttac atgttgctt tagaattgt agcgtatga 
      tttattaaa taaaaattt acgagaaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY