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Gene information
General information
TC012783 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 14685426..14704371
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012783
    iBeetle screen iB_05317

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Pupal morphology
    beetle stock: bad (increased lethality),
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012783 RA
      MLLILSTFL AFADAVYFS VSDINITIP IDKGKFPLN 
      KQPLASFLA QGAPPLNYT ISNNLLRIS PSGDVFFSP 
      DFLKSDITN VDAISARIS VRDNSNKEA TSYLNLEFV 
      LLESLDCDL IVEELCFWK TVRYNFSEN AKPARIGSL 
      ASPFLVEMC ASCEINYDL LSKNKEFRV VSSGALHSI 
      ESTEALDRE TQSLHLLQV SCTVENSKH TLRNINQTV 
      AVAVGDVDD NPPKAQERS IRINMSSKH VHKDQVVAH 
      KDLIFSDKD SPAVNKYKP VILNDTLKI LKAQCNKFS 
      DDHLKQEVQ TAVHCKLKF TKTVPVKTS TYSVVLQLN 
      DSSLIHGNG AVGMPIDLQ FLDDSGYHP ALYKRQLRP 
      ATLYPSNRI QIFRTAAPL ARLTQPRYL HGASNFTLK 
      PILPKHYAI FNVTYIEGI IFVHDSENL RNATEFVRL 
      NISWTQNDS RKSDEIQVQ IVDEPTKTC NDVRKFNDW 
      EHCAQYTTP EECLSPRAC ALATGGSSS VEKRKRPQR 
      CMWRGEKTP SNEPTTLYA TCTPDLETC PDEKCDELE 
      QLESTVCPQ DCAEEVLFP AERNKMTGR GIFRGSGVC 
      FCSTLQCHC NPLEYVKLP KRRKPTKPP ETTPFLANS 
      SRHTSNATK IMGIELAKC GTTCIFGVI GGALFLGAA 
      VALFVICWK LDSVHKAVR DKFGEENQD LAAPLSDYT 
      MPEPMPLNF EMTTSLADT AILNIINKY APDPKWEFP 
      RNRLIIEQT LGEGEFGKV LRAKALNIS GQPGETTVA 
      VKTLKDDAR ESELNDLLS EYQLLKEVS HPNVIRLLG 
      VCTAPGGPI YLIIEFAEH GSLRNYLRR SRHLKSECG 
      RLPSSTVDE NDVHYDEPN ISKVTPKEI LSFAWQICK 
      GMAYLSEIK LVHRDLAAR NVLLAADQI CKISDFGLT 
      RDIYEDDAY FKRSKGRVP VKWMAPESL SDHIYTNKS 
      DVWSFGILV WELVTLGAT PYPGIAVQN LFHLLRQGY 
      RMERPDNCS PTLYNIMRS CWHIDPEQR PTFQELAAL 
      WEKMLSDEL FLRSVPTKR RRRIRLTTY PKSQSYIKC 
      GSADKFQES ADFLAEPDN NQNDINARG YETPVKVPK 
      KVQTPTNDC PQYYTDMAS GSST
    CDS sequence
    • >TC012783 RA
      atgttacta atactttcc acttttttg gcgttcgcg 
      gacgcggtc tatttcagc gtgagcgat atcaacatc 
      accatcccc attgacaag ggcaagttc cccttgaac 
      aagcagccc ttggccagt ttcctcgcc cagggcgcc 
      cctccgctc aactacacc atcagcaat aacctcctc 
      agaatcagc ccgagcggc gatgtcttc ttcagcccc 
      gatttcctc aaatcggac atcacaaac gtggatgcg 
      atctcggcc cgcatctca gtgcgagat aactcgaat 
      aaggaagct acaagttac ttaaacttg gagtttgtc 
      ttattggag tcgctcgat tgcgatctg atcgtcgaa 
      gagttgtgt ttttggaag acagttcgg tacaatttc 
      agcgaaaat gcaaaaccg gcccgaatc gggtcactg 
      gcttcgccg tttttggtg gaaatgtgc gcaagttgt 
      gaaattaac tacgaccta ctcagcaaa aacaaggag 
      tttagggtc gtttcatcg ggggcttta cactcgatt 
      gagtcgacg gaggctctc gatagggag acgcaatcg 
      ctacactta ttgcaagtt tcgtgcacc gttgaaaat 
      tccaaacac acacttagg aatatcaac cagacggtg 
      gctgtggcg gtgggagac gtcgatgat aatcccccg 
      aaagcgcaa gagaggagc attagaata aacatgtca 
      tccaagcat gtccataag gatcaagtc gtagcacac 
      aaagattta attttcagc gacaaagat tcgcccgct 
      gttaataaa tacaagccc gtgatttta aacgatacg 
      ctgaaaatc ctgaaagct cagtgcaac aagttttcc 
      gatgaccac ttgaagcag gaagtccaa actgctgtc 
      cattgcaaa ttgaaattc acgaaaacc gttcctgtg 
      aaaacatca acttattcc gtcgtttta caattaaac 
      gactcctcc cttattcac gggaatggg gcagttggt 
      atgccgatt gatttgcaa ttcctggat gattcggga 
      tatcatccc gcgttgtat aaaagacag ctgagaccg 
      gcaacgctt tatcccagc aacaggatt cagattttc 
      aggactgca gccccctta gcccgcctg actcagccc 
      cgctacctc cacggcgcc tcaaacttc acccttaag 
      cccatcctc cccaaacac tacgccata ttcaacgtg 
      acctacatc gaaggaata attttcgtc cacgattcg 
      gaaaatcta cggaacgca actgaattc gtgagactg 
      aacatttcc tggacgcaa aacgattct cgcaaatcg 
      gatgaaatc caggtccag atcgtcgac gagcccaca 
      aagacttgc aacgacgtg cgaaaattc aacgactgg 
      gagcactgc gcccagtac accaccccc gaggagtgc 
      ctgagcccc agagcctgc gccctggcc accgggggc 
      tcctcgagc gtggagaag cggaagcgg ccccagagg 
      tgcatgtgg cgcggggag aaaaccccc tccaacgaa 
      cccaccaca ctctacgcc acgtgcacg cccgacttg 
      gagacatgt cctgacgaa aagtgcgac gagttggag 
      cagttggag agcacggtg tgtccgcaa gactgcgcc 
      gaagaggtg ctgtttcca gcagagcgg aataaaatg 
      accggtcgt gggattttt cgcggaagt ggggtctgt 
      ttttgcagc actttgcag tgccactgc aaccctctg 
      gagtacgtc aagttgccg aaacggcgc aagccgaca 
      aaacccccc gaaaccacc cctttcctg gccaactcc 
      agccggcat accagcaat gcgaccaag atcatgggc 
      atcgagctg gccaagtgc ggcaccacg tgcattttt 
      ggggtcatc ggaggtgcg ctgttcctg ggggccgct 
      gtggcgttg tttgttatt tgctggaag cttgacagc 
      gtgcataag gccgttagg gacaagttc ggggaggag 
      aaccaggac ctggcggcc cctctgtcc gattatacg 
      atgcccgag cccatgccg cttaatttc gaaatgact 
      acttcgctt gctgatacc gcaattttg aacatcatc 
      aacaagtat gcgcctgac cccaagtgg gaattcccc 
      aggaatcga ctcattatc gagcaaacg ctcggagag 
      ggcgagttc gggaaggtg ttgcgggcc aaggcgctc 
      aacatttca ggacaacca ggtgagacg accgtcgcc 
      gtgaaaacc ctgaaagac gacgcgcgc gaatccgag 
      ctcaacgac ttactctcc gaataccaa ctccttaag 
      gaagtttcg cacccgaac gtaattcgg cttctgggt 
      gtttgcacc gctccaggt ggcccaatt tacctgatt 
      atagagttt gccgaacac ggatcgctc cgaaattat 
      ttgaggagg agtagacac ctcaagtcg gagtgtggc 
      cgccttccc agctccaca gtcgacgaa aatgacgtc 
      cactacgac gagccgaac atctccaaa gtgacaccg 
      aaggaaatc ctgtccttt gcctggcag atatgcaag 
      gggatggcg tacttaagc gagattaaa ctggttcat 
      cgcgacctg gcggcgcga aatgtgctc ctggcggcc 
      gaccagatt tgcaaaatt tccgacttt ggcctgact 
      agagacatt tacgaggat gatgcctat ttcaagagg 
      agcaaaggg agagttccc gtgaagtgg atggcgccg 
      gagtcgctc tccgaccac atctacacc aataaatcg 
      gacgtctgg agcttcggc atcctggtg tgggagttg 
      gtgactttg ggcgcgacg ccttatccc gggatcgcc 
      gtccagaac ctcttccac ttgctgcgc cagggctac 
      cggatggag cgtcccgac aactgttcc ccaaccctc 
      tacaacatc atgcggagc tgctggcac atcgacccc 
      gaacagagg cccactttc caggagctg gcggcgctc 
      tgggagaag atgctgagc gacgagtta tttctgcgc 
      tccgttccg acgaaaagg cggagacga atccggtta 
      actacttat cccaagtcg cagtcgtat atcaagtgc 
      ggatcggcg gataagttt caggaaagt gcggatttt 
      ctcgccgag ccggataac aatcagaat gatatcaat 
      gcaagaggg tatgagacg cccgtcaag gtgcccaag 
      aaggtgcaa acgcctacg aatgactgt ccgcagtat 
      tacactgat atggcgtcg gggtcgagc acttga
    mRNA sequence
    • >TC012783 RA
      tcgtgcgtc ggaacgtcc aacagccgg tgatttttg 
      gcgcgaaag tgcgacttt tcagtgcga caatgttac 
      taatacttt ccacttttt tggcgttcg cggacgcgg 
      tctatttca gcgtgagcg atatcaaca tcaccatcc 
      ccattgaca agggcaagt tccccttga acaagcagc 
      ccttggcca gtttcctcg cccagggcg cccctccgc 
      tcaactaca ccatcagca ataacctcc tcagaatca 
      gcccgagcg gcgatgtct tcttcagcc ccgatttcc 
      tcaaatcgg acatcacaa acgtggatg cgatctcgg 
      cccgcatct cagtgcgag ataactcga ataaggaag 
      ctacaagtt acttaaact tggagtttg tcttattgg 
      agtcgctcg attgcgatc tgatcgtcg aagagttgt 
      gtttttgga agacagttc ggtacaatt tcagcgaaa 
      atgcaaaac cggcccgaa tcgggtcac tggcttcgc 
      cgtttttgg tggaaatgt gcgcaagtt gtgaaatta 
      actacgacc tactcagca aaaacaagg agtttaggg 
      tcgtttcat cgggggctt tacactcga ttgagtcga 
      cggaggctc tcgataggg agacgcaat cgctacact 
      tattgcaag tttcgtgca ccgttgaaa attccaaac 
      acacactta ggaatatca accagacgg tggctgtgg 
      cggtgggag acgtcgatg ataatcccc cgaaagcgc 
      aagagagga gcattagaa taaacatgt catccaagc 
      atgtccata aggatcaag tcgtagcac acaaagatt 
      taattttca gcgacaaag attcgcccg ctgttaata 
      aatacaagc ccgtgattt taaacgata cgctgaaaa 
      tcctgaaag ctcagtgca acaagtttt ccgatgacc 
      acttgaagc aggaagtcc aaactgctg tccattgca 
      aattgaaat tcacgaaaa ccgttcctg tgaaaacat 
      caacttatt ccgtcgttt tacaattaa acgactcct 
      cccttattc acgggaatg gggcagttg gtatgccga 
      ttgatttgc aattcctgg atgattcgg gatatcatc 
      ccgcgttgt ataaaagac agctgagac cggcaacgc 
      tttatccca gcaacagga ttcagattt tcaggactg 
      cagccccct tagcccgcc tgactcagc cccgctacc 
      tccacggcg cctcaaact tcaccctta agcccatcc 
      tccccaaac actacgcca tattcaacg tgacctaca 
      tcgaaggaa taattttcg tccacgatt cggaaaatc 
      tacggaacg caactgaat tcgtgagac tgaacattt 
      cctggacgc aaaacgatt ctcgcaaat cggatgaaa 
      tccaggtcc agatcgtcg acgagccca caaagactt 
      gcaacgacg tgcgaaaat tcaacgact gggagcact 
      gcgcccagt acaccaccc ccgaggagt gcctgagcc 
      ccagagcct gcgccctgg ccaccgggg gctcctcga 
      gcgtggaga agcggaagc ggccccaga ggtgcatgt 
      ggcgcgggg agaaaaccc cctccaacg aacccacca 
      cactctacg ccacgtgca cgcccgact tggagacat 
      gtcctgacg aaaagtgcg acgagttgg agcagttgg 
      agagcacgg tgtgtccgc aagactgcg ccgaagagg 
      tgctgtttc cagcagagc ggaataaaa tgaccggtc 
      gtgggattt ttcgcggaa gtggggtct gtttttgca 
      gcactttgc agtgccact gcaaccctc tggagtacg 
      tcaagttgc cgaaacggc gcaagccga caaaacccc 
      ccgaaacca cccctttcc tggccaact ccagccggc 
      ataccagca atgcgacca agatcatgg gcatcgagc 
      tggccaagt gcggcacca cgtgcattt ttggggtca 
      tcggaggtg cgctgttcc tgggggccg ctgtggcgt 
      tgtttgtta tttgctgga agcttgaca gcgtgcata 
      aggccgtta gggacaagt tcggggagg agaaccagg 
      acctggcgg cccctctgt ccgattata cgatgcccg 
      agcccatgc cgcttaatt tcgaaatga ctacttcgc 
      ttgctgata ccgcaattt tgaacatca tcaacaagt 
      atgcgcctg accccaagt gggaattcc ccaggaatc 
      gactcatta tcgagcaaa cgctcggag agggcgagt 
      tcgggaagg tgttgcggg ccaaggcgc tcaacattt 
      caggacaac caggtgaga cgaccgtcg ccgtgaaaa 
      ccctgaaag acgacgcgc gcgaatccg agctcaacg 
      acttactct ccgaatacc aactcctta aggaagttt 
      cgcacccga acgtaattc ggcttctgg gtgtttgca 
      ccgctccag gtggcccaa tttacctga ttatagagt 
      ttgccgaac acggatcgc tccgaaatt atttgagga 
      ggagtagac acctcaagt cggagtgtg gccgccttc 
      ccagctcca cagtcgacg aaaatgacg tccactacg 
      acgagccga acatctcca aagtgacac cgaaggaaa 
      tcctgtcct ttgcctggc agatatgca aggggatgg 
      cgtacttaa gcgagatta aactggttc atcgcgacc 
      tggcggcgc gaaatgtgc tcctggcgg ccgaccaga 
      tttgcaaaa tttccgact ttggcctga ctagagaca 
      tttacgagg atgatgcct atttcaaga ggagcaaag 
      ggagagttc ccgtgaagt ggatggcgc cggagtcgc 
      tctccgacc acatctaca ccaataaat cggacgtct 
      ggagcttcg gcatcctgg tgtgggagt tggtgactt 
      tgggcgcga cgccttatc ccgggatcg ccgtccaga 
      acctcttcc acttgctgc gccagggct accggatgg 
      agcgtcccg acaactgtt ccccaaccc tctacaaca 
      tcatgcgga gctgctggc acatcgacc ccgaacaga 
      ggcccactt tccaggagc tggcggcgc tctgggaga 
      agatgctga gcgacgagt tatttctgc gctccgttc 
      cgacgaaaa ggcggagac gaatccggt taactactt 
      atcccaagt cgcagtcgt atatcaagt gcggatcgg 
      cggataagt ttcaggaaa gtgcggatt ttctcgccg 
      agccggata acaatcaga atgatatca atgcaagag 
      ggtatgaga cgcccgtca aggtgccca agaaggtgc 
      aaacgccta cgaatgact gtccgcagt attacactg 
      atatggcgt cggggtcga gcacttgag ggcgttttg 
      gagcggaac ggaatctta ttatcaaat tttgtattg 
      tagtaggta gggtggtat taggagaag agttactaa 
      aaaataata aaacgacta tttttatgt acttaacta 
      agcaacaat taaataaag gcattatta cgactgctt 
      gatattacg tcaatgtat tattgtatc gaaatataa 
      ttaaaaata aattgttta ttacgtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY