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Gene information
General information
TC012779 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 14616281..14628205
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC012779
    iBeetle screen iB_05316

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    beetle stock: bad (increased lethality),
    Pupal morphology
    beetle stock: bad (increased lethality),
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    mandible not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012779 RA
      MPSSPEAVQ NPLEKDLTT KEEQPTKTH 
NTIEELKTK LTNIETEES AIEMIPESQ 
RGPTHHPPP PHLNEDGLI IPRKPPNPV 
KENSERQNL HKELLFNQK IGKNVLNQK 
SELQKALEK QKENLAKKQ LENHLADKT 
PELEKVIAD RAKRLQGNT NEEKNEDDK 
VINKEFLQA RMKLKNRSD SK
    • >TC012779 RB
      MIPESQRGP THHPPPPHL NEDGLIIPR 
KPPNPVKEN SERQNLHKE LLFNQKIGK 
NVLNQKSEL QKALEKQKE NLAKKQLEN 
HLADKTPEL EKVIADRAK RLQGNTNEE 
KNEDDKVIN KEFLQARMK LKNRSDSK
    CDS sequence
    • >TC012779 RA
      atgccgagt tctcctgaa gccgtacaa aatccgctc 
gaaaaggat ttgacaacg aaagaagaa caacccacc 
aaaacccac aacaccatc gaggagctc aaaaccaaa 
ctaactaac atagaaaca gaagagtct gccatcgag 
atgattccg gagagtcag agagggcca acgcaccac 
cccccaccg ccccatctc aacgaggac ggcttgata 
attccccgg aaaccgcca aatccggtg aaggaaaac 
agcgagcgc cagaatctc cacaaagag ttgttattt 
aatcagaaa attgggaaa aatgtactc aaccaaaaa 
tccgaattg caaaaagcg ctcgaaaaa cagaaggag 
aatttggcg aagaaacaa ctggagaat catttggct 
gataaaaca ccagagttg gagaaggtg atagctgat 
agggcgaag cggttgcag ggcaacacc aacgaggag 
aagaacgag gacgacaaa gtgatcaac aaggagttc 
ctccaggct cgaatgaag ctgaagaac cgcagcgat 
tccaaataa
    • >TC012779 RB
      atgattccg gagagtcag agagggcca acgcaccac 
cccccaccg ccccatctc aacgaggac ggcttgata 
attccccgg aaaccgcca aatccggtg aaggaaaac 
agcgagcgc cagaatctc cacaaagag ttgttattt 
aatcagaaa attgggaaa aatgtactc aaccaaaaa 
tccgaattg caaaaagcg ctcgaaaaa cagaaggag 
aatttggcg aagaaacaa ctggagaat catttggct 
gataaaaca ccagagttg gagaaggtg atagctgat 
agggcgaag cggttgcag ggcaacacc aacgaggag 
aagaacgag gacgacaaa gtgatcaac aaggagttc 
ctccaggct cgaatgaag ctgaagaac cgcagcgat 
tccaaataa
    mRNA sequence
    • >TC012779 RA
      ttgagtaaa aaaactgtc aaagtttgt ttataaatg 
gcgtcacgg gttttttgt tgtgttatt ttctgatgt 
ggttttttg ttgtttgaa atttttgtt tgagtgaaa 
ttgagtgta gacaaattc cagtggttg gtgcgagtt 
ttcagctta ttgatttcc gggtcttgg ctgaaaatc 
ggatgaaag tgggcggaa actgggccg gaatgagat 
ataatcaaa taaaatgcc gagttctcc tgaagccgt 
acaaaatcc gctcgaaaa ggatttgac aacgaaaga 
agaacaacc caccaaaac ccacaacac catcgagga 
gctcaaaac caaactaac taacataga aacagaaga 
gtctgccat cgagatgat tccggagag tcagagagg 
gccaacgca ccacccccc accgcccca tctcaacga 
ggacggctt gataattcc ccggaaacc gccaaatcc 
ggtgaagga aaacagcga gcgccagaa tctccacaa 
agagttgtt atttaatca gaaaattgg gaaaaatgt 
actcaacca aaaatccga attgcaaaa agcgctcga 
aaaacagaa ggagaattt ggcgaagaa acaactgga 
gaatcattt ggctgataa aacaccaga gttggagaa 
ggtgatagc tgatagggc gaagcggtt gcagggcaa 
caccaacga ggagaagaa cgaggacga caaagtgat 
caacaagga gttcctcca ggctcgaat gaagctgaa 
gaaccgcag cgattccaa ataagactt gacgcattt 
ccgtatcaa atcttaggt ttatttaac tgttttctt 
attttatgt gataagtat taggtgtac tcattagga 
cttgtgtaa tatgtatca tactatgca ataaaatta 
ttttttcta c
    • >TC012779 RB
      gtattctat tattagtca gggacgcca ccgatgatg 
attacgaat aggatttga caacgaaag aagaacaac 
ccaccaaaa cccacaaca ccatcgagg agctcaaaa 
ccaaactaa ctaacatag aaacaggtg agcgatcga 
tttttccgg cgtaattcc gcacttgac ctattgcaa 
atccgatta ttcggcctt gactagcct ggcttcgct 
ccggatgat tttttttac cattttatt ttagaagag 
tctgccatc gagatgatt ccggagagt cagagaggg 
ccaacgcac cacccccca ccgccccat ctcaacgag 
gacggcttg ataattccc cggaaaccg ccaaatccg 
gtgaaggaa aacagcgag cgccagaat ctccacaaa 
gagttgtta tttaatcag aaaattggg aaaaatgta 
ctcaaccaa aaatccgaa ttgcaaaaa gcgctcgaa 
aaacagaag gagaatttg gcgaagaaa caactggag 
aatcatttg gctgataaa acaccagag ttggagaag 
gtgatagct gatagggcg aagcggttg cagggcaac 
accaacgag gagaagaac gaggacgac aaagtgatc 
aacaaggag ttcctccag gctcgaatg aagctgaag 
aaccgcagc gattccaaa taagacttg acgcatttc 
cgtatcaaa tcttaggtt tatttaact gttttctta 
ttttatgtg ataagtatt aggtgtact cattaggac 
ttgtgtaat atgtatcat actatgcaa taaaattat 
tttttctac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY