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Gene information
General information
TC012772 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 14532116..14533178
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012772
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05315

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Pupal morphology
    beetle stock: bad (increased lethality),
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    about 25

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012772 RA
      MHPVWILCG VIATIYALL KHYLKSTVW CRSKTCLVG 
      KTAVVTGAN TGIGYETAL DFAKRGARV ILACRDQQK 
      ADAAKTRIV QETGNGDIV VKIVDFASF DSVRAFAQN 
      VKETEQRLD ILVNNAGAS GIPDKKSKD GLNLLMQVN 
      YFSSFLLTN LLIDLLKKS EPSRVVNVS SLAAKYARF 
      DLEDLNRFT GMHTDYANS KLCNVLFTM ELAEKLQGT 
      RVTTFSLHP GVVDTEIFR RIKGQHKAV FEFFRDHFF 
      RTSEEGAQT TIYCSVERN IEDLSGEHF DNCERVEPY 
      KTSQVKGLA KKLWEKSEK FVELKPEEV HF
    CDS sequence
    • >TC012772 RA
      atgcaccca gtttggatt ttgtgcggc gtcattgcc 
      accatttat gcccttttg aagcactac ctcaaatcc 
      acggtgtgg tgccgcagc aaaacgtgt ctagttggc 
      aaaaccgcg gtcgtcaca ggcgcaaac actgggatt 
      ggttacgag acggcgcta gatttcgcc aaaaggggc 
      gcccgcgtg attctggct tgccgggac cagcagaaa 
      gccgacgct gctaaaacc cgaatcgtt caagaaacg 
      gggaatggg gacattgtg gtgaaaatc gtcgatttt 
      gcctcgttt gattcagtc cgagcgttt gcacaaaac 
      gtaaaagag acggaacag cgtctcgat atcctggtc 
      aacaatgcc ggagcttcc ggaattccg gacaaaaaa 
      agcaaagac gggctgaac cttttaatg caagtcaac 
      tacttctcc agtttcctg ctcacgaac ctcctcatc 
      gacttgctc aaaaaatca gagcccagt cgcgtagtt 
      aacgtgtct tctttggcc gcgaaatac gcacgcttc 
      gatctggaa gacttgaac cgattcacc ggaatgcac 
      acggattat gccaacagc aagctttgc aatgttttg 
      ttcacgatg gaactggcg gaaaagctg caagggacg 
      agggttacg accttttcg ctgcatccc ggagtcgtg 
      gacacggaa atcttccgg aggattaag ggacaacac 
      aaggccgtg tttgagttt ttcagggat cattttttc 
      aggacgagc gaggaaggg gcacaaact acgatctat 
      tgctcggtt gagaggaat attgaggat ttgagtggc 
      gaacatttc gataattgt gaaagagtt gagccttac 
      aagacctcg caggtgaaa gggttggct aagaagctg 
      tgggagaag tcggagaag tttgtcgag ttgaagccc 
      gaagaagtg cacttttga
    mRNA sequence
    • >TC012772 RA
      tgtgggtga atacgcttc aattgtgtg ctaatctcg 
      gctcgaaat gcacccagt ttggatttt gtgcggcgt 
      cattgccac catttatgc ccttttgaa gcactacct 
      caaatccac ggtgtggtg ccgcagcaa aacgtgtct 
      agttggcaa aaccgcggt cgtcacagg cgcaaacac 
      tgggattgg ttacgagac ggcgctaga tttcgccaa 
      aaggggcgc ccgcgtgat tctggcttg ccgggacca 
      gcagaaagc cgacgctgc taaaacccg aatcgttca 
      agaaacggg gaatgggga cattgtggt gaaaatcgt 
      cgattttgc ctcgtttga ttcagtccg agcgtttgc 
      acaaaacgt aaaagagac ggaacagcg tctcgatat 
      cctggtcaa caatgccgg agcttccgg aattccgga 
      caaaaaaag caaagacgg gctgaacct tttaatgca 
      agtcaacta cttctccag tttcctgct cacgaacct 
      cctcatcga cttgctcaa aaaatcaga gcccagtcg 
      cgtagttaa cgtgtcttc tttggccgc gaaatacgc 
      acgcttcga tctggaaga cttgaaccg attcaccgg 
      aatgcacac ggattatgc caacagcaa gctttgcaa 
      tgttttgtt cacgatgga actggcgga aaagctgca 
      agggacgag ggttacgac cttttcgct gcatcccgg 
      agtcgtgga cacggaaat cttccggag gattaaggg 
      acaacacaa ggccgtgtt tgagttttt cagggatca 
      ttttttcag gacgagcga ggaaggggc acaaactac 
      gatctattg ctcggttga gaggaatat tgaggattt 
      gagtggcga acatttcga taattgtga aagagttga 
      gccttacaa gacctcgca ggtgaaagg gttggctaa 
      gaagctgtg ggagaagtc ggagaagtt tgtcgagtt 
      gaagcccga agaagtgca cttttgata taataaagt 
      tgcaaaaat cgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY