Status
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Gene information
General information
TC034448
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC012763,TC012762 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 14323847..14372077
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC034448
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02017

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 22.22% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Pupal morphology
    elytra proximal partiallyblistered - Number of animals showing the phenotype: 7

    Adult morphology
    gula surface irregular - Number of animals showing the phenotype: 4


    elytra partially blistered - Number of animals showing the phenotype: 4


    hindwing shape irregular - Number of animals showing the phenotype: 4

    Fertility
    egg production: number <50% of expected -
    vitellogenic egg chamber slightly number decreased - Number of animals showing the phenotype: 2

    tropharium slightly shape irregular - Number of animals showing the phenotype: 2

    ovary without detectable defects - Number of animals showing the phenotype: 1
    iBeetle screen iB_05313

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Fertility
    egg chamber not present - Number of animals showing the phenotype: 3

    egg chamber mostly not present - Number of animals showing the phenotype: 1

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    larva lethality late (L7) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034448 RA
      MGLGVSKEA PCPELERPE SVISSKLHG RQASIRQST 
MRAKSKQPM PDPNELERR FTKVLASMD LPPDKAKLL 
KNYDSEKKW DIICDQEMV HAKDPPSFY LNKLRTYLD 
PKASRSHRK RKMVGESTS TQVLRDLEI SLRTNHIEW 
VREFLNEEN NGLDVLIDY LSFRLGMMR HEQRIAESR 
TASEERLGP GQPPPAPSV QNGYIRAPL DIPDSSSVK 
RRSKHVAKL NMGESKDDI HVCIMCMRA IMNNKYGFN 
MVIQHREAI NCISLSLIH KSLRTKALV LELLAAICL 
VKGGHEIIL SAFDNFKEV CHEEHRFQT LMDYFLNYD 
VFHIEFMVA CMQFVNIIV HSVEDMNFR VHLQYEFTA 
LGLDSYLEK LRFTESEEL QVQISAYLD NVFDVAALM 
EDSETKTAA LEKVAEMED ELAQLHEKI QDIEHQALE 
EIAHLKTDL AQLRQERDE LAEFKRHAD EEVNTLRRV 
VQHHEQESK NRQSMFESK IAELEKTVP KSASVAGIS 
DLTPPDSTT SPVPASPVI PPPAAPPPP PCPPPPPSR 
VPSAPVPPP PAGFMQAPD GAMTIKRKV QTKYKLPTI 
NWIALKPNQ VRGTIFNEL DDDKLHGFI DFNDFEERF 
KIGNAGLLA NGTSELDGL STFPSKRFK KPENVTLLE 
HTRLRNIAI SRRKLEMSP EKVINAINL LDLKQLSLE 
NVELLQRMV PTEQETKAY REYVNDKKN VNLLTEEDK 
FLLQLTKVE RISAKLSIM SYIGNFYDN LHLITPQTH 
AIISGSNSV KNSKKLRSV LEIILAFGN YLNSSKRGP 
AYGFKLQSL DTLLDTKST DKRMCLLHY IVATIRQKF 
PELLNFDSD LHYIEKAAV VSLENITTD VHELEKGME 
AVRKEAEIR GKGPQSIVV RDFLANAEE KLRRLRNDA 
KTAQEAFRE CVEYFAESP RTTDANTFF SLLVRFVKA 
FKQADQDNE QRRRLEIAA QNANNKTNE DVAKRNKIN 
QKKQQEAVI NELKHKTRL VQEKKLLQQ DEVYNGALE 
DILLGLKSE PYRRADAVR RSQRRRIDN NRLSKTMEE 
LEV
    CDS sequence
    • >TC034448 RA
      atgggcttg ggcgtatcg aaggaggcg ccttgcccg 
gagctggaa aggccggaa agtgtgatc tcgtcgaaa 
ttgcacgga agacaggcg agcatccgc cagagcacc 
atgagggcc aagtcgaag cagcccatg cccgacccc 
aacgaactg gagaggaga ttcaccaaa gtgctggcc 
tccatggac ttgccgccc gataaagcc aaactgctg 
aaaaactac gattcggag aagaaatgg gacatcatc 
tgcgatcag gaaatggtg cacgctaaa gaccctccc 
tccttctac ttaaacaaa ctccggacg tacctcgac 
cccaaggcc tcccgcagc cacaggaag cgaaaaatg 
gtcggcgag tcgacttcg actcaagtc ctgcgtgac 
ctggagata tccctgcgg acgaaccac atcgaatgg 
gtgcgagaa tttttaaac gaggagaac aacggcttg 
gacgtcctg atcgactat ttgagcttt aggctgggc 
atgatgcgg cacgagcag cgcatcgcc gagtccagg 
accgcctcc gaggagcgg ctgggcccc ggccagccg 
ccgcccgcc cccagcgtc cagaacgga tacattcgc 
gcccccttg gacatcccg gacagctcg agcgtcaaa 
cggcgctcc aagcacgtc gccaagctg aacatgggg 
gagtccaag gacgacata cacgtctgc atcatgtgc 
atgagggcc attatgaac aataagtat gggttcaat 
atggtgatc cagcaccgt gaggccatc aactgcatt 
tcgctcagc ttgatccac aagagtttg cgcacgaag 
gcgctggtg ctggagctt ttggcggcg atttgcctg 
gtcaaaggc gggcacgag ataattctc tccgcgttt 
gataatttc aaagaggtg tgtcatgag gagcaccgc 
ttccagacg ttgatggat tattttctc aattacgat 
gtcttccac atcgagttt atggttgcg tgcatgcag 
tttgtgaac attattgtg cattcggtg gaggatatg 
aactttagg gtccatctg cagtatgag ttcacggcg 
ctggggctg gacagctat ttggagaag ttgaggttt 
acggaaagt gaggagttg caagtgcag atctcggcc 
tatttggac aacgtgttc gatgtggcg gcgctcatg 
gaggatagc gagaccaag acggcggcc ttggagaag 
gtggccgag atggaggac gagctggcg caactccac 
gaaaaaatc caagacatc gaacaccaa gccctcgaa 
gagatcgcc cacttgaaa accgacctg gcccaactg 
cgccaagaa cgcgacgaa ctcgccgaa ttcaaacgc 
cacgccgac gaagaggtc aacacttta cgtcgcgtt 
gtccaacac cacgaacaa gaatcgaaa aatcgccaa 
tcgatgttc gaatcaaaa atcgccgaa ctcgaaaaa 
acagtcccg aagtcggcc tcagttgcc ggtatttcc 
gacctgacg ccgcccgat tcgaccacc tcgcctgtg 
cccgcctcg cctgtgata ccgcccccg gccgcgccg 
ccgccgccg ccgtgcccg ccgccgccg ccgtccaga 
gtgccttcc gcgcccgtg ccgccgccg cccgccggc 
ttcatgcaa gcgccagat ggcgccatg accatcaaa 
cgcaaagta caaaccaaa tacaaactg ccaaccatc 
aattggatc gcactgaag ccgaatcaa gtcagaggg 
acgattttt aacgaattg gacgatgat aagctgcac 
ggattcatc gattttaac gactttgag gagcgattc 
aagattgga aatgccggc ttgttggcg aatggcact 
agtgagctt gatgggctt tcgacgttc ccgagcaaa 
aggttcaaa aagccggaa aatgtcaca cttttggag 
cacaccaga ctgagaaat atcgcaatt tcgaggcga 
aaattggaa atgtcgcct gagaaagtg ataaatgcg 
atcaattta ctcgatttg aaacaattg agtctcgaa 
aatgtcgaa ttgttgcaa aggatggtg ccgaccgag 
caggaaaca aaagcctat cgggagtat gtcaatgat 
aagaaaaac gtcaatttg ttgactgaa gaggacaag 
tttttgcta cagttgacc aaggtcgag cgaatttcg 
gccaagttg tcgattatg agttacata ggcaatttt 
tacgacaat ctgcacttg ataacgccg caaacacat 
gcgatcatt tctggctca aattctgtg aaaaactcg 
aagaaattg agatcagtt ttggagata atcctcgca 
ttcggcaat tatctcaac agcagtaaa cgagggccg 
gcttacggc ttcaaactt caatcactc gacacactt 
ttagacacg aaatcgacc gataaaaga atgtgtctt 
ttgcattac atcgtcgca acgattcgc caaaaattc 
cccgaattg ctaaacttc gactcggac ctgcactac 
atagagaaa gccgcagtt gtttctctc gaaaacatc 
acaactgat gtccatgag ctagagaaa gggatggaa 
gcggtccga aaagaggcc gaaatccgg ggcaaaggg 
cctcagagc atcgtcgtt cgtgatttc ctcgccaat 
gccgaggag aagttgagg cgattgagg aacgacgcc 
aaaacagca caagaggcc ttcagggag tgcgtggag 
tattttgca gagtcgcca aggaccacc gatgccaac 
actttcttc tccttgtta gtcagattt gtcaaagct 
ttcaagcaa gcggatcag gacaacgag caaagaagg 
agactggaa atcgctgct caaaatgcc aacaataaa 
accaacgaa gacgtggcg aagaggaat aaaatcaac 
cagaagaaa caacaagaa gctgtaata aacgaattg 
aaacacaaa acaagactg gtgcaggag aaaaagctc 
ttgcaacaa gacgaagtg tacaatggt gcccttgaa 
gatatcctc ctagggcta aaaagtgag ccttaccgt 
cgtgccgat gcggtgcga agaagtcaa agacggagg 
attgataat aatcgcttg tctaaaaca atggaagag 
ttggaagtt tga
    mRNA sequence
    • >TC034448 RA
      ctttttttg cgaatgcgc tattacata acctcacag 
tgtttacaa acaaaaaat tgtaaaaac ttatcaaaa 
cacggaaga taagtcaac aaatcgcaa aaaatggat 
caacaaaca acccccaac ggttccggg ttcgtttat 
ttcaacacg agttcaaat caagaatat caaaaccaa 
tcggacacg gctgacttt ttggccttt actgacact 
ccccaagcg tctcctccc actaacaag tttaaacct 
gcttactac agcagcccc cagtcgaaa ttccacagg 
gggagcccc cgacaatac ccccaaaat aatcggagg 
tatcagcgt tggtcgggg ggctataac cggtcttac 
aatagctca aactccagt tttaaccag agtggctca 
tttagggag agtgaccaa gagggccct aatatctta 
aaattcata catccgtcg tttctggag gacccctgg 
gcctccttg gaggccaaa ttgaaggaa aaggggcag 
tttgacgat tcgtcgcaa tcagggtcc caggaaact 
tagcaattt tagcgtcaa aaaaaaata caaattgtg 
caaattaat taattttaa ttgtaggtt taaaaaatt 
gatatttta ttaaaaaca cagttaaga aaatagttt 
ttgagcgac agattcctg tattaccgg cccagtatc 
ccgtttttt gtgtcaaga tggcgcccg aatcccaat 
gtaaaaatt ccatcccaa acatgcgca catctctct 
tggaaaatc taaaacttt gtttagttt tgcgagatt 
tacgtttgc ggcgcgttg tgtgaaaac ctgttgcgt 
ttgtgtgac gcgatcgtg cgtgatgcg gcaaagaac 
gtgattttt ggtggcgca tagtgtatt ttttctgcg 
accatgggc ttgggcgta tcgaaggag gcgccttgc 
ccggagctg gaaaggccg gaaagtgtg atctcgtcg 
aaattgcac ggaagacag gcgagcatc cgccagagc 
accatgagg gccaagtcg aagcagccc atgcccgac 
cccaacgaa ctggagagg agattcacc aaagtgctg 
gcctccatg gacttgccg cccgataaa gccaaactg 
ctgaaaaac tacgattcg gagaagaaa tgggacatc 
atctgcgat caggaaatg gtgcacgct aaagaccct 
ccctccttc tacttaaac aaactccgg acgtacctc 
gaccccaag gcctcccgc agccacagg aagcgaaaa 
atggtcggc gagtcgact tcgactcaa gtcctgcgt 
gacctggag atatccctg cggacgaac cacatcgaa 
tgggtgcga gaattttta aacgaggag aacaacggc 
ttggacgtc ctgatcgac tatttgagc tttaggctg 
ggcatgatg cggcacgag cagcgcatc gccgagtcc 
aggaccgcc tccgaggag cggctgggc cccggccag 
ccgccgccc gcccccagc gtccagaac ggatacatt 
cgcgccccc ttggacatc ccggacagc tcgagcgtc 
aaacggcgc tccaagcac gtcgccaag ctgaacatg 
ggggagtcc aaggacgac atacacgtc tgcatcatg 
tgcatgagg gccattatg aacaataag tatgggttc 
aatatggtg atccagcac cgtgaggcc atcaactgc 
atttcgctc agcttgatc cacaagagt ttgcgcacg 
aaggcgctg gtgctggag cttttggcg gcgatttgc 
ctggtcaaa ggcgggcac gagataatt ctctccgcg 
tttgataat ttcaaagag gtgtgtcat gaggagcac 
cgcttccag acgttgatg gattatttt ctcaattac 
gatgtcttc cacatcgag tttatggtt gcgtgcatg 
cagtttgtg aacattatt gtgcattcg gtggaggat 
atgaacttt agggtccat ctgcagtat gagttcacg 
gcgctgggg ctggacagc tatttggag aagttgagg 
tttacggaa agtgaggag ttgcaagtg cagatctcg 
gcctatttg gacaacgtg ttcgatgtg gcggcgctc 
atggaggat agcgagacc aagacggcg gccttggag 
aaggtggcc gagatggag gacgagctg gcgcaactc 
cacgaaaaa atccaagac atcgaacac caagccctc 
gaagagatc gcccacttg aaaaccgac ctggcccaa 
ctgcgccaa gaacgcgac gaactcgcc gaattcaaa 
cgccacgcc gacgaagag gtcaacact ttacgtcgc 
gttgtccaa caccacgaa caagaatcg aaaaatcgc 
caatcgatg ttcgaatca aaaatcgcc gaactcgaa 
aaaacagtc ccgaagtcg gcctcagtt gccggtatt 
tccgacctg acgccgccc gattcgacc acctcgcct 
gtgcccgcc tcgcctgtg ataccgccc ccggccgcg 
ccgccgccg ccgccgtgc ccgccgccg ccgccgtcc 
agagtgcct tccgcgccc gtgccgccg ccgcccgcc 
ggcttcatg caagcgcca gatggcgcc atgaccatc 
aaacgcaaa gtacaaacc aaatacaaa ctgccaacc 
atcaattgg atcgcactg aagccgaat caagtcaga 
gggacgatt tttaacgaa ttggacgat gataagctg 
cacggattc atcgatttt aacgacttt gaggagcga 
ttcaagatt ggaaatgcc ggcttgttg gcgaatggc 
actagtgag cttgatggg ctttcgacg ttcccgagc 
aaaaggttc aaaaagccg gaaaatgtc acacttttg 
gagcacacc agactgaga aatatcgca atttcgagg 
cgaaaattg gaaatgtcg cctgagaaa gtgataaat 
gcgatcaat ttactcgat ttgaaacaa ttgagtctc 
gaaaatgtc gaattgttg caaaggatg gtgccgacc 
gagcaggaa acaaaagcc tatcgggag tatgtcaat 
gataagaaa aacgtcaat ttgttgact gaagaggac 
aagtttttg ctacagttg accaaggtc gagcgaatt 
tcggccaag ttgtcgatt atgagttac ataggcaat 
ttttacgac aatctgcac ttgataacg ccgcaaaca 
catgcgatc atttctggc tcaaattct gtgaaaaac 
tcgaagaaa ttgagatca gttttggag ataatcctc 
gcattcggc aattatctc aacagcagt aaacgaggg 
ccggcttac ggcttcaaa cttcaatca ctcgacaca 
cttttagac acgaaatcg accgataaa agaatgtgt 
cttttgcat tacatcgtc gcaacgatt cgccaaaaa 
ttccccgaa ttgctaaac ttcgactcg gacctgcac 
tacatagag aaagccgca gttgtttct ctcgaaaac 
atcacaact gatgtccat gagctagag aaagggatg 
gaagcggtc cgaaaagag gccgaaatc cggggcaaa 
gggcctcag agcatcgtc gttcgtgat ttcctcgcc 
aatgccgag gagaagttg aggcgattg aggaacgac 
gccaaaaca gcacaagag gccttcagg gagtgcgtg 
gagtatttt gcagagtcg ccaaggacc accgatgcc 
aacactttc ttctccttg ttagtcaga tttgtcaaa 
gctttcaag caagcggat caggacaac gagcaaaga 
aggagactg gaaatcgct gctcaaaat gccaacaat 
aaaaccaac gaagacgtg gcgaagagg aataaaatc 
aaccagaag aaacaacaa gaagctgta ataaacgaa 
ttgaaacac aaaacaaga ctggtgcag gagaaaaag 
ctcttgcaa caagacgaa gtgtacaat ggtgccctt 
gaagatatc ctcctaggg ctaaaaagt gagccttac 
cgtcgtgcc gatgcggtg cgaagaagt caaagacgg 
aggattgat aataatcgc ttgtctaaa acaatggaa 
gagttggaa gtttgagtc tcaaaagct gtaaataat 
tcgtgtaaa tgctctctg atgctgatg atgatatta 
agatgtgat tgtatttaa atatttttg tacatagtt 
gcaatttta taaagtact actagtggt aaggctatt 
tttatattg ttgtttgta cactgaaat aatcattac 
ggcacattc cgggtttgt cgtttgtgc gtgttttac 
tgatttgtg attttgaaa tgttttaat gcgaattgt 
atgtttatt atttatagc caagcaata aaaagttta 
ttattgatt gattaaaag ttgggaatt atttcgtgc 
cttttccct cctttgaaa cttttccct tttttctat 
tttagcttt tgttgtcaa acaaaaaaa cgaacatac 
ggacttttt ttgactttt aacaactgt agaggaacg 
taaggacgt aacaagtcc aaaaaccta cgatagaac 
gagttttaa aaaaaattt tttttcacc tttttgaag 
gtaaaagtg tattgaaat ttaagcaaa aaaattgaa 
aattttaaa tctcgtgaa aagttggta taatacaga 
aaatgaaca gctgaattc aatggaaca aaccgcatt 
gcgctacga cttttagtt tttgagtta tgaattttt 
ttaatgaat aaaagtttc cactataga tattgaaca 
gctcgagaa ggttgtaaa ggcatataa aagtccata 
aaactatgt tagaatgag ttaaaaaaa atctttttt 
catttttgt gaaggccat taaaatatt aaacaaaaa 
aatttagat cgggccaat tgacacttt tgtactttg 
aaaggttca aaatggcga aaaacgttt ttggggcga 
aaaagaaaa aataccttc ttgaccaac ttttaaaaa 
ttggggttg tcggggatg cattgaaag gcatttaaa 
ttatttaaa catttttat tgtaaaatg acaaagaat 
aaatctaaa cttttcaga atcataata tgtttgata 
taggtagta ctcgaatac atgatacag attgtaaaa 
actgaacgc aagtttcaa aataatatt aaaaatata 
taaacgcat cacttcgaa ctggccgtg tcattgtac 
atataatga atactgctg gaacgagcc tcagccgct 
gaaggaggt ctcgatcgt gtctcggcc gccttgaaa 
caacggtgc aaaatagaa tttcgtact gtacgtaat 
ttgataaaa atccaaatt gtgcccata gtttaagct 
aaactttac caactacgt taatctaaa agtaatcta 
agtatgtaa atagtagag tatatgtgg atagataga 
tatacgcat cagctatgt cgatattta tgttgaatg 
ca
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