Status
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Gene information
General information
TC012758 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 14302787..14308395
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012758
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05312

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012758 RA
      MTYDHPNNN AVIYKNEKM KKRCRSCCH VCLLLLGIA 
      LIGLGLAIF AYFEPLYDF MMTSAMTFT PKTEPFRVW 
      RKNDPPLDM DIYLFNWTN PQDLHVPGV KPRFQEVGP 
      YRFKEVKEK INITWHDNN NTISYKHKK SYFFDAKNS 
      VRQLNDVIN TINVVPLTI AYKARNFGF FSKRTISYS 
      LSTLSSLYV TKTAGEILF DGYEESILS ILSTVPLAG 
      VVDKFGLFY GKNNSMGTD GIFSMWTKV DENFGKIVT 
      WNYKNESDF YEGKCNAVK GSAGEFYPI NQKRDYIEF 
      FSSELCKFA KLEYEKDVN VKGVLGYKY TARHIFDNG 
      TLRPENKCF CSGECHPSG VFNVSKCRE NSPTFLSMP 
      HFYGADPYY YNTIEGLKP EDKHEFYIT LEPKSGIVL 
      DIGARMQVN VLLQPIEHI TMYADVPKL FIPLFYFDQ 
      SLPATDELA SLLIKIQSL PEVSNIAVM ILVTLGLAS 
      FVWVVVMYC WSKKRRHKT ELKIPQSNV EEIPLRC
    CDS sequence
    • >TC012758 RA
      atgacgtac gaccatccg aacaacaat gctgtgata 
      tacaagaat gaaaagatg aagaaaaga tgccgatcc 
      tgctgccat gtctgcctc cttctcctg ggaatagca 
      ctaatcgga ctaggactc gcgattttc gcctacttt 
      gaacccctt tacgacttt atgatgaca agtgccatg 
      acgttcaca ccaaagact gaaccgttc agagtctgg 
      aggaaaaac gatccgccc ctcgacatg gacatctat 
      ctcttcaac tggaccaac ccgcaggac ctccacgtc 
      ccgggggtc aagcccagg ttccaggaa gtcgggcct 
      tacaggttc aaggaagtg aaggagaag atcaacatc 
      acgtggcac gacaacaac aacaccatc agctacaag 
      cacaaaaaa tcctacttt ttcgatgcg aaaaacagc 
      gtgcggcag ctcaatgac gtcataaac accatcaat 
      gtcgtgcct ttgacaata gcgtacaaa gcccgcaat 
      ttcggcttt ttctccaaa cgcacaatt tcctactcc 
      ctctcgacg ttatctagt ttatacgtg acaaaaacg 
      gcgggcgag atcctcttc gatggttac gaagagagc 
      attttgagc atactaagt acggtcccc ctggcagga 
      gtcgttgat aagttcgga ttgttttac gggaaaaat 
      aactcaatg ggaaccgac ggaattttc agcatgtgg 
      acgaaagtc gatgaaaat ttcggaaaa attgtgacc 
      tggaactac aaaaacgag tcggacttt tacgaaggg 
      aagtgtaac gctgtgaag ggctcagcg ggagagttt 
      taccccatc aaccaaaag cgcgactac atcgaattc 
      ttctcctcg gaattgtgc aaattcgcc aaactcgaa 
      tacgaaaag gacgtcaac gtgaagggg gttttgggg 
      tacaaatac accgcgaga cacatcttc gacaatggt 
      acgttgcga ccggaaaat aaatgtttt tgttcgggc 
      gaatgccac ccttctggc gttttcaac gtgtcaaag 
      tgtcgcgaa aactcgccg acttttcta tcaatgccg 
      catttctac ggtgccgat ccttattat tataacacc 
      atcgaggga ttaaaaccc gaggacaaa cacgaattt 
      tacatcact ttggaaccg aaatcggga atagttctg 
      gatatcggg gcccggatg caagtcaac gtgcttctg 
      cagccaatc gaacacatt acaatgtac gccgacgtc 
      cccaagctc ttcatcccc ttgttctac ttcgaccag 
      agcctcccc gcaaccgac gaattggct tctctcctg 
      atcaaaatc cagagtctt ccggaagtg agcaacatt 
      gccgtcatg atattagtc actttaggc ctcgcttcg 
      ttcgtctgg gtggtcgta atgtactgc tggtcgaaa 
      aaacgacga cacaaaacc gaattgaaa atacctcaa 
      agtaacgtc gaagaaatc ccgcttcgt tgttga
    mRNA sequence
    • >TC012758 RA
      tgtttaaaa aaccgaaaa cattttgtt gagtgtgtc 
      tttggtggg ttgttgact cgtttgctt cagttttat 
      tatcaaaag tttagtgaa aggttatca gatgtgcca 
      agtacgagc gaactaagt ttgttcaag gaaactatc 
      gatttcaaa aatgccaca atcgggata ataaatcaa 
      atatgtgga ggactgcgc gctcgttcc aagaaataa 
      tttaataat tcgagggag agagtgatg gaccttttg 
      ttgctcttt aatgaacgc aacgagtgc ggcccatga 
      cgtacgacc atccgaaca acaatgctg tgatataca 
      agaatgaaa agatgaaga aaagatgcc gatcctgct 
      gccatgtct gcctccttc tcctgggaa tagcactaa 
      tcggactag gactcgcga ttttcgcct actttgaac 
      ccctttacg actttatga tgacaagtg ccatgacgt 
      tcacaccaa agactgaac cgttcagag tctggagga 
      aaaacgatc cgcccctcg acatggaca tctatctct 
      tcaactgga ccaacccgc aggacctcc acgtcccgg 
      gggtcaagc ccaggttcc aggaagtcg ggccttaca 
      ggttcaagg aagtgaagg agaagatca acatcacgt 
      ggcacgaca acaacaaca ccatcagct acaagcaca 
      aaaaatcct actttttcg atgcgaaaa acagcgtgc 
      ggcagctca atgacgtca taaacacca tcaatgtcg 
      tgcctttga caatagcgt acaaagccc gcaatttcg 
      gctttttct ccaaacgca caatttcct actccctct 
      cgacgttat ctagtttat acgtgacaa aaacggcgg 
      gcgagatcc tcttcgatg gttacgaag agagcattt 
      tgagcatac taagtacgg tccccctgg caggagtcg 
      ttgataagt tcggattgt tttacggga aaaataact 
      caatgggaa ccgacggaa ttttcagca tgtggacga 
      aagtcgatg aaaatttcg gaaaaattg tgacctgga 
      actacaaaa acgagtcgg acttttacg aagggaagt 
      gtaacgctg tgaagggct cagcgggag agttttacc 
      ccatcaacc aaaagcgcg actacatcg aattcttct 
      cctcggaat tgtgcaaat tcgccaaac tcgaatacg 
      aaaaggacg tcaacgtga agggggttt tggggtaca 
      aatacaccg cgagacaca tcttcgaca atggtacgt 
      tgcgaccgg aaaataaat gtttttgtt cgggcgaat 
      gccaccctt ctggcgttt tcaacgtgt caaagtgtc 
      gcgaaaact cgccgactt ttctatcaa tgccgcatt 
      tctacggtg ccgatcctt attattata acaccatcg 
      agggattaa aacccgagg acaaacacg aattttaca 
      tcactttgg aaccgaaat cgggaatag ttctggata 
      tcggggccc ggatgcaag tcaacgtgc ttctgcagc 
      caatcgaac acattacaa tgtacgccg acgtcccca 
      agctcttca tccccttgt tctacttcg accagagcc 
      tccccgcaa ccgacgaat tggcttctc tcctgatca 
      aaatccaga gtcttccgg aagtgagca acattgccg 
      tcatgatat tagtcactt taggcctcg cttcgttcg 
      tctgggtgg tcgtaatgt actgctggt cgaaaaaac 
      gacgacaca aaaccgaat tgaaaatac ctcaaagta 
      acgtcgaag aaatcccgc ttcgttgtt gaaagctga 
      ttttaatgt ttacgatgt acctacact tttacagtg 
      taatacgat aattattgt tactttagt ttttattta 
      tattaaaaa cgtaaaaag gag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY