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Gene information
General information
TC012756 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 14293227..14299969
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012756
    iBeetle screen iB_05311

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Pupal morphology
    beetle stock: bad (increased lethality),
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012756 RA
      MCERTADQV TRRTYSMKS TRCLPKCVC AFVVSVVFI 
      VGGFVLPFI WPVILKYLI ESNMVLMPT SHAFDIWRK 
      FPTAFPIDF YFFNWTNPE DVYNSSVKM KFREMGPYR 
      YSETKEKAD IVWNKNGTV SFRHLKFWY ENREKGNIN 
      DEITTVNPV ALSAAYSAR TWGYLIRQG LSLSLSSMS 
      PTVHITRSV SEMLFTGYK DPLITLARS LPFLSGSLP 
      PWDKFGWFY TRNGSAHYE GIFNMGTGI NSTFGRLYS 
      WNYWTQTPY YEGSCAMVN GSAGEFFTK LDNKSISFF 
      SPDLCRTMT LRYSGQSVV NNILGNKYV VDSYMLDNG 
      TIFPENRCF CNGECVPSG LVNVSSCRF GSPSFASLP 
      HFYQADAYY TDSIEGVRP EKSKHEFFL TLEPTTGIP 
      LEVSARLQI NLLMQPDSG ISLYKGVPK VFVPILWFE 
      QKIRIPDGE AFKLRLLLN LPVICTAFG IILVFAGVI 
      VVSLLVYKI CTMNLCGKR KEKLLYADQ SIPLKMDRS 
      LEIVKKI
    • >TC012756 RB
      MVTRRTYSM KSTRCLPKC VCAFVVSVV FIVGGFVLP 
      FIWPVILKY LIESNMVLM PTSHAFDIW RKFPTAFPI 
      DFYFFNWTN PEDVYNSSV KMKFREMGP YRYSETKEK 
      ADIVWNKNG TVSFRHLKF WYENREKGN INDEITTVN 
      PVALSAAYS ARTWGYLIR QGLSLSLSS MSPTVHITR 
      SVSEMLFTG YKDPLITLA RSLPFLSGS LPPWDKFGW 
      FYTRNGSAH YEGIFNMGT GINSTFGRL YSWNYWTQT 
      PYYEGSCAM VNGSAGEFF TKLDNKSIS FFSPDLCRT 
      MTLRYSGQS VVNNILGNK YVVDSYMLD NGTIFPENR 
      CFCNGECVP SGLVNVSSC RFGSPSFAS LPHFYQADA 
      YYTDSIEGV RPEKSKHEF FLTLEPTTG IPLEVSARL 
      QINLLMQPD SGISLYKGV PKVFVPILW FEQKIRIPD 
      GEAFKLRLL LNLPVICTA FGIILVFAG VIVVSLLVY 
      KICTMNLCG KRKEKLLYA DQSIPLKMD RSLEIVKKI
    CDS sequence
    • >TC012756 RA
      atgtgcgag cggaccgca gaccaggtc accagaaga 
      acctacagc atgaagtcc acgaggtgt ctgccaaag 
      tgtgtgtgc gcgtttgtg gtgtcggtg gtttttatt 
      gtcggcggt tttgtgctt ccgtttatc tggccagtg 
      attttgaaa tatttaatc gagagtaac atggtgtta 
      atgccgact tcgcacgct tttgatatc tggaggaag 
      tttccgacg gcgttcccc atcgatttc tacttcttc 
      aactggacg aatccagag gacgtgtac aacagttcg 
      gtgaaaatg aaatttcgg gaaatgggg ccttatcgg 
      tactcggag acgaaagaa aaggccgat atcgtttgg 
      aataaaaac gggacggtt tcgttcaga cacttgaag 
      ttttggtac gaaaatcgg gagaaagga aatatcaac 
      gacgaaatc accactgta aatccagtc gctttatca 
      gctgcctac tccgcgcgc acgtggggt tacttaatc 
      cgccaaggc ctctcctta tccctgtcc agcatgtcc 
      ccaactgtc cacatcact aggtccgtg tcagagatg 
      cttttcacc ggctacaag gaccctctg atcaccctt 
      gcccgctcc ttgcccttt ctatcgggc tctttgcca 
      ccttgggac aaattcggt tggttctac acccggaac 
      ggatcagcg cattacgaa ggcattttt aacatggga 
      actggaata aactccact tttggtaga ctgtacagt 
      tggaattat tggacacaa acgccttat tacgagggc 
      tcttgtgcc atggttaat ggttcagcg ggggaattt 
      ttcaccaag cttgataat aaatcgatt agttttttc 
      tcgccggat ttgtgccgg accatgacg ctcaggtac 
      tccgggcag agcgtcgtg aataatata ctagggaac 
      aagtacgtc gtcgacagt tacatgctc gataatggc 
      acgattttc ccggaaaac cgctgtttc tgcaacggc 
      gaatgcgtg ccttcgggc ctagtcaac gtttcgagc 
      tgtcgtttc ggctcacca tcattcgcc tcactgccg 
      catttctac caagcagat gcctactat accgactca 
      atcgaaggc gttcgcccg gaaaaatcc aagcacgag 
      tttttcctc accctggag ccaaccacc ggaattccg 
      ttggaagtc agcgcccgg ctccaaatt aatctacta 
      atgcagccc gattccggg atatcgttg tacaaaggc 
      gtccctaaa gtttttgtg cccattcta tggttcgag 
      cagaaaatt cggattccg gacggtgaa gctttcaag 
      ttgagatta ctcctcaat cttccggtg atctgcacc 
      gctttcggg attatcctc gtgtttgct ggcgtgatt 
      gttgtgtcg ttacttgtt tataaaata tgcacgatg 
      aatttgtgc ggcaagaga aaagagaag ttgttgtac 
      gccgaccaa agtattcca ctcaaaatg gaccggtcg 
      ctcgaaatt gttaaaaag atttga
    • >TC012756 RB
      atggtcacc agaagaacc tacagcatg aagtccacg 
      aggtgtctg ccaaagtgt gtgtgcgcg tttgtggtg 
      tcggtggtt tttattgtc ggcggtttt gtgcttccg 
      tttatctgg ccagtgatt ttgaaatat ttaatcgag 
      agtaacatg gtgttaatg ccgacttcg cacgctttt 
      gatatctgg aggaagttt ccgacggcg ttccccatc 
      gatttctac ttcttcaac tggacgaat ccagaggac 
      gtgtacaac agttcggtg aaaatgaaa tttcgggaa 
      atggggcct tatcggtac tcggagacg aaagaaaag 
      gccgatatc gtttggaat aaaaacggg acggtttcg 
      ttcagacac ttgaagttt tggtacgaa aatcgggag 
      aaaggaaat atcaacgac gaaatcacc actgtaaat 
      ccagtcgct ttatcagct gcctactcc gcgcgcacg 
      tggggttac ttaatccgc caaggcctc tccttatcc 
      ctgtccagc atgtcccca actgtccac atcactagg 
      tccgtgtca gagatgctt ttcaccggc tacaaggac 
      cctctgatc acccttgcc cgctccttg ccctttcta 
      tcgggctct ttgccacct tgggacaaa ttcggttgg 
      ttctacacc cggaacgga tcagcgcat tacgaaggc 
      atttttaac atgggaact ggaataaac tccactttt 
      ggtagactg tacagttgg aattattgg acacaaacg 
      ccttattac gagggctct tgtgccatg gttaatggt 
      tcagcgggg gaatttttc accaagctt gataataaa 
      tcgattagt tttttctcg ccggatttg tgccggacc 
      atgacgctc aggtactcc gggcagagc gtcgtgaat 
      aatatacta gggaacaag tacgtcgtc gacagttac 
      atgctcgat aatggcacg attttcccg gaaaaccgc 
      tgtttctgc aacggcgaa tgcgtgcct tcgggccta 
      gtcaacgtt tcgagctgt cgtttcggc tcaccatca 
      ttcgcctca ctgccgcat ttctaccaa gcagatgcc 
      tactatacc gactcaatc gaaggcgtt cgcccggaa 
      aaatccaag cacgagttt ttcctcacc ctggagcca 
      accaccgga attccgttg gaagtcagc gcccggctc 
      caaattaat ctactaatg cagcccgat tccgggata 
      tcgttgtac aaaggcgtc cctaaagtt tttgtgccc 
      attctatgg ttcgagcag aaaattcgg attccggac 
      ggtgaagct ttcaagttg agattactc ctcaatctt 
      ccggtgatc tgcaccgct ttcgggatt atcctcgtg 
      tttgctggc gtgattgtt gtgtcgtta cttgtttat 
      aaaatatgc acgatgaat ttgtgcggc aagagaaaa 
      gagaagttg ttgtacgcc gaccaaagt attccactc 
      aaaatggac cggtcgctc gaaattgtt aaaaagatt 
      tga
    mRNA sequence
    • >TC012756 RA
      ccgatcgaa aatgcgacg agaaaaata ttgatttta 
      aaacgtaac aaacgtgcg gtaaataaa tattggcgc 
      aaaatgggc aaaacgctc tttcggtta cgtaattgt 
      tattgagat gaaagtcat cattttttc taatagtgt 
      gcactttaa atacaatgc aagtttgcc gcaagtgca 
      atgcgcgat gcaactgca tttgaacaa tttgataag 
      ttgagatgt gcgagcgga ccgcagacc aggtcacca 
      gaagaacct acagcatga agtccacga ggtgtctgc 
      caaagtgtg tgtgcgcgt ttgtggtgt cggtggttt 
      ttattgtcg gcggttttg tgcttccgt ttatctggc 
      cagtgattt tgaaatatt taatcgaga gtaacatgg 
      tgttaatgc cgacttcgc acgcttttg atatctgga 
      ggaagtttc cgacggcgt tccccatcg atttctact 
      tcttcaact ggacgaatc cagaggacg tgtacaaca 
      gttcggtga aaatgaaat ttcgggaaa tggggcctt 
      atcggtact cggagacga aagaaaagg ccgatatcg 
      tttggaata aaaacggga cggtttcgt tcagacact 
      tgaagtttt ggtacgaaa atcgggaga aaggaaata 
      tcaacgacg aaatcacca ctgtaaatc cagtcgctt 
      tatcagctg cctactccg cgcgcacgt ggggttact 
      taatccgcc aaggcctct ccttatccc tgtccagca 
      tgtccccaa ctgtccaca tcactaggt ccgtgtcag 
      agatgcttt tcaccggct acaaggacc ctctgatca 
      cccttgccc gctccttgc cctttctat cgggctctt 
      tgccacctt gggacaaat tcggttggt tctacaccc 
      ggaacggat cagcgcatt acgaaggca tttttaaca 
      tgggaactg gaataaact ccacttttg gtagactgt 
      acagttgga attattgga cacaaacgc cttattacg 
      agggctctt gtgccatgg ttaatggtt cagcggggg 
      aatttttca ccaagcttg ataataaat cgattagtt 
      ttttctcgc cggatttgt gccggacca tgacgctca 
      ggtactccg ggcagagcg tcgtgaata atatactag 
      ggaacaagt acgtcgtcg acagttaca tgctcgata 
      atggcacga ttttcccgg aaaaccgct gtttctgca 
      acggcgaat gcgtgcctt cgggcctag tcaacgttt 
      cgagctgtc gtttcggct caccatcat tcgcctcac 
      tgccgcatt tctaccaag cagatgcct actataccg 
      actcaatcg aaggcgttc gcccggaaa aatccaagc 
      acgagtttt tcctcaccc tggagccaa ccaccggaa 
      ttccgttgg aagtcagcg cccggctcc aaattaatc 
      tactaatgc agcccgatt ccgggatat cgttgtaca 
      aaggcgtcc ctaaagttt ttgtgccca ttctatggt 
      tcgagcaga aaattcgga ttccggacg gtgaagctt 
      tcaagttga gattactcc tcaatcttc cggtgatct 
      gcaccgctt tcgggatta tcctcgtgt ttgctggcg 
      tgattgttg tgtcgttac ttgtttata aaatatgca 
      cgatgaatt tgtgcggca agagaaaag agaagttgt 
      tgtacgccg accaaagta ttccactca aaatggacc 
      ggtcgctcg aaattgtta aaaagattt gaattgttg 
      taaccgaat tgttattta taatagata attattatt 
      gtaatttgt tatgttaat aaaaatttg tttctatt
    • >TC012756 RB
      ccgatcgaa aatgcgacg agaaaaata ttgatttta 
      aaacgtaac aaacgtgcg gtaaataaa tattggcgc 
      aaaatgggc aaaacgctc tttcggtta cgtaattgt 
      tattgagat gaaagtcat cattttttc taatagtgt 
      gcactttaa atacaatgc aagtttgcc gcaagtgca 
      atgcgcgat gcaactgca tttgaacaa tttgataag 
      ttgagatgt gcgagcgga ccgcagacc agcgcaatt 
      cgctggtag taaacatag ccttgagac cactcattt 
      ccaccttga taatgaact agataaaac cataaaaac 
      gtaaaaatc acagtgata acctatcaa tagattgtg 
      tgtgctggt ccaaaaatc ggtcaatct catgtgagt 
      taatcaaat catctcgta taatcgcgt gcacgccca 
      catgtcatc ttttccgtt caatattca aattttccg 
      tccgagcgt attagccac cctataatc gtggtgttt 
      tcctcaaag cggtgcagc cactggccg ataccttaa 
      taatttaat ccaatttcc tccggtata aagccacca 
      tcaggcaca ttgcatcag caagtcggt aaaaagctt 
      ccaattagt ccgaatttg tgatttcgc gtaaagtga 
      attaacgag gggtcaaat ggtgcttca cctccgact 
      taataacaa gcactcgct cggatcttt attaggttg 
      ttgttctaa accatgaag tgcagctaa atgccagcc 
      atggtcacc agaagaacc tacagcatg aagtccacg 
      aggtgtctg ccaaagtgt gtgtgcgcg tttgtggtg 
      tcggtggtt tttattgtc ggcggtttt gtgcttccg 
      tttatctgg ccagtgatt ttgaaatat ttaatcgag 
      agtaacatg gtgttaatg ccgacttcg cacgctttt 
      gatatctgg aggaagttt ccgacggcg ttccccatc 
      gatttctac ttcttcaac tggacgaat ccagaggac 
      gtgtacaac agttcggtg aaaatgaaa tttcgggaa 
      atggggcct tatcggtac tcggagacg aaagaaaag 
      gccgatatc gtttggaat aaaaacggg acggtttcg 
      ttcagacac ttgaagttt tggtacgaa aatcgggag 
      aaaggaaat atcaacgac gaaatcacc actgtaaat 
      ccagtcgct ttatcagct gcctactcc gcgcgcacg 
      tggggttac ttaatccgc caaggcctc tccttatcc 
      ctgtccagc atgtcccca actgtccac atcactagg 
      tccgtgtca gagatgctt ttcaccggc tacaaggac 
      cctctgatc acccttgcc cgctccttg ccctttcta 
      tcgggctct ttgccacct tgggacaaa ttcggttgg 
      ttctacacc cggaacgga tcagcgcat tacgaaggc 
      atttttaac atgggaact ggaataaac tccactttt 
      ggtagactg tacagttgg aattattgg acacaaacg 
      ccttattac gagggctct tgtgccatg gttaatggt 
      tcagcgggg gaatttttc accaagctt gataataaa 
      tcgattagt tttttctcg ccggatttg tgccggacc 
      atgacgctc aggtactcc gggcagagc gtcgtgaat 
      aatatacta gggaacaag tacgtcgtc gacagttac 
      atgctcgat aatggcacg attttcccg gaaaaccgc 
      tgtttctgc aacggcgaa tgcgtgcct tcgggccta 
      gtcaacgtt tcgagctgt cgtttcggc tcaccatca 
      ttcgcctca ctgccgcat ttctaccaa gcagatgcc 
      tactatacc gactcaatc gaaggcgtt cgcccggaa 
      aaatccaag cacgagttt ttcctcacc ctggagcca 
      accaccgga attccgttg gaagtcagc gcccggctc 
      caaattaat ctactaatg cagcccgat tccgggata 
      tcgttgtac aaaggcgtc cctaaagtt tttgtgccc 
      attctatgg ttcgagcag aaaattcgg attccggac 
      ggtgaagct ttcaagttg agattactc ctcaatctt 
      ccggtgatc tgcaccgct ttcgggatt atcctcgtg 
      tttgctggc gtgattgtt gtgtcgtta cttgtttat 
      aaaatatgc acgatgaat ttgtgcggc aagagaaaa 
      gagaagttg ttgtacgcc gaccaaagt attccactc 
      aaaatggac cggtcgctc gaaattgtt aaaaagatt 
      tgaattgtt gtaaccgaa ttgttattt ataatagat 
      aattattat tgtaatttg ttatgttaa taaaaattt 
      gtttctatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY