| General information | |||
| TC012756 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 14293227..14299969 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC012756 | ||
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05311 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
- >TC012756 RA
MCERTADQV TRRTYSMKS TRCLPKCVC AFVVSVVFI VGGFVLPFI WPVILKYLI ESNMVLMPT SHAFDIWRK FPTAFPIDF YFFNWTNPE DVYNSSVKM KFREMGPYR YSETKEKAD IVWNKNGTV SFRHLKFWY ENREKGNIN DEITTVNPV ALSAAYSAR TWGYLIRQG LSLSLSSMS PTVHITRSV SEMLFTGYK DPLITLARS LPFLSGSLP PWDKFGWFY TRNGSAHYE GIFNMGTGI NSTFGRLYS WNYWTQTPY YEGSCAMVN GSAGEFFTK LDNKSISFF SPDLCRTMT LRYSGQSVV NNILGNKYV VDSYMLDNG TIFPENRCF CNGECVPSG LVNVSSCRF GSPSFASLP HFYQADAYY TDSIEGVRP EKSKHEFFL TLEPTTGIP LEVSARLQI NLLMQPDSG ISLYKGVPK VFVPILWFE QKIRIPDGE AFKLRLLLN LPVICTAFG IILVFAGVI VVSLLVYKI CTMNLCGKR KEKLLYADQ SIPLKMDRS LEIVKKI
- >TC012756 RB
MVTRRTYSM KSTRCLPKC VCAFVVSVV FIVGGFVLP FIWPVILKY LIESNMVLM PTSHAFDIW RKFPTAFPI DFYFFNWTN PEDVYNSSV KMKFREMGP YRYSETKEK ADIVWNKNG TVSFRHLKF WYENREKGN INDEITTVN PVALSAAYS ARTWGYLIR QGLSLSLSS MSPTVHITR SVSEMLFTG YKDPLITLA RSLPFLSGS LPPWDKFGW FYTRNGSAH YEGIFNMGT GINSTFGRL YSWNYWTQT PYYEGSCAM VNGSAGEFF TKLDNKSIS FFSPDLCRT MTLRYSGQS VVNNILGNK YVVDSYMLD NGTIFPENR CFCNGECVP SGLVNVSSC RFGSPSFAS LPHFYQADA YYTDSIEGV RPEKSKHEF FLTLEPTTG IPLEVSARL QINLLMQPD SGISLYKGV PKVFVPILW FEQKIRIPD GEAFKLRLL LNLPVICTA FGIILVFAG VIVVSLLVY KICTMNLCG KRKEKLLYA DQSIPLKMD RSLEIVKKI
- >TC012756 RA
atgtgcgag cggaccgca gaccaggtc accagaaga acctacagc atgaagtcc acgaggtgt ctgccaaag tgtgtgtgc gcgtttgtg gtgtcggtg gtttttatt gtcggcggt tttgtgctt ccgtttatc tggccagtg attttgaaa tatttaatc gagagtaac atggtgtta atgccgact tcgcacgct tttgatatc tggaggaag tttccgacg gcgttcccc atcgatttc tacttcttc aactggacg aatccagag gacgtgtac aacagttcg gtgaaaatg aaatttcgg gaaatgggg ccttatcgg tactcggag acgaaagaa aaggccgat atcgtttgg aataaaaac gggacggtt tcgttcaga cacttgaag ttttggtac gaaaatcgg gagaaagga aatatcaac gacgaaatc accactgta aatccagtc gctttatca gctgcctac tccgcgcgc acgtggggt tacttaatc cgccaaggc ctctcctta tccctgtcc agcatgtcc ccaactgtc cacatcact aggtccgtg tcagagatg cttttcacc ggctacaag gaccctctg atcaccctt gcccgctcc ttgcccttt ctatcgggc tctttgcca ccttgggac aaattcggt tggttctac acccggaac ggatcagcg cattacgaa ggcattttt aacatggga actggaata aactccact tttggtaga ctgtacagt tggaattat tggacacaa acgccttat tacgagggc tcttgtgcc atggttaat ggttcagcg ggggaattt ttcaccaag cttgataat aaatcgatt agttttttc tcgccggat ttgtgccgg accatgacg ctcaggtac tccgggcag agcgtcgtg aataatata ctagggaac aagtacgtc gtcgacagt tacatgctc gataatggc acgattttc ccggaaaac cgctgtttc tgcaacggc gaatgcgtg ccttcgggc ctagtcaac gtttcgagc tgtcgtttc ggctcacca tcattcgcc tcactgccg catttctac caagcagat gcctactat accgactca atcgaaggc gttcgcccg gaaaaatcc aagcacgag tttttcctc accctggag ccaaccacc ggaattccg ttggaagtc agcgcccgg ctccaaatt aatctacta atgcagccc gattccggg atatcgttg tacaaaggc gtccctaaa gtttttgtg cccattcta tggttcgag cagaaaatt cggattccg gacggtgaa gctttcaag ttgagatta ctcctcaat cttccggtg atctgcacc gctttcggg attatcctc gtgtttgct ggcgtgatt gttgtgtcg ttacttgtt tataaaata tgcacgatg aatttgtgc ggcaagaga aaagagaag ttgttgtac gccgaccaa agtattcca ctcaaaatg gaccggtcg ctcgaaatt gttaaaaag atttga
- >TC012756 RB
atggtcacc agaagaacc tacagcatg aagtccacg aggtgtctg ccaaagtgt gtgtgcgcg tttgtggtg tcggtggtt tttattgtc ggcggtttt gtgcttccg tttatctgg ccagtgatt ttgaaatat ttaatcgag agtaacatg gtgttaatg ccgacttcg cacgctttt gatatctgg aggaagttt ccgacggcg ttccccatc gatttctac ttcttcaac tggacgaat ccagaggac gtgtacaac agttcggtg aaaatgaaa tttcgggaa atggggcct tatcggtac tcggagacg aaagaaaag gccgatatc gtttggaat aaaaacggg acggtttcg ttcagacac ttgaagttt tggtacgaa aatcgggag aaaggaaat atcaacgac gaaatcacc actgtaaat ccagtcgct ttatcagct gcctactcc gcgcgcacg tggggttac ttaatccgc caaggcctc tccttatcc ctgtccagc atgtcccca actgtccac atcactagg tccgtgtca gagatgctt ttcaccggc tacaaggac cctctgatc acccttgcc cgctccttg ccctttcta tcgggctct ttgccacct tgggacaaa ttcggttgg ttctacacc cggaacgga tcagcgcat tacgaaggc atttttaac atgggaact ggaataaac tccactttt ggtagactg tacagttgg aattattgg acacaaacg ccttattac gagggctct tgtgccatg gttaatggt tcagcgggg gaatttttc accaagctt gataataaa tcgattagt tttttctcg ccggatttg tgccggacc atgacgctc aggtactcc gggcagagc gtcgtgaat aatatacta gggaacaag tacgtcgtc gacagttac atgctcgat aatggcacg attttcccg gaaaaccgc tgtttctgc aacggcgaa tgcgtgcct tcgggccta gtcaacgtt tcgagctgt cgtttcggc tcaccatca ttcgcctca ctgccgcat ttctaccaa gcagatgcc tactatacc gactcaatc gaaggcgtt cgcccggaa aaatccaag cacgagttt ttcctcacc ctggagcca accaccgga attccgttg gaagtcagc gcccggctc caaattaat ctactaatg cagcccgat tccgggata tcgttgtac aaaggcgtc cctaaagtt tttgtgccc attctatgg ttcgagcag aaaattcgg attccggac ggtgaagct ttcaagttg agattactc ctcaatctt ccggtgatc tgcaccgct ttcgggatt atcctcgtg tttgctggc gtgattgtt gtgtcgtta cttgtttat aaaatatgc acgatgaat ttgtgcggc aagagaaaa gagaagttg ttgtacgcc gaccaaagt attccactc aaaatggac cggtcgctc gaaattgtt aaaaagatt tga
- >TC012756 RA
ccgatcgaa aatgcgacg agaaaaata ttgatttta aaacgtaac aaacgtgcg gtaaataaa tattggcgc aaaatgggc aaaacgctc tttcggtta cgtaattgt tattgagat gaaagtcat cattttttc taatagtgt gcactttaa atacaatgc aagtttgcc gcaagtgca atgcgcgat gcaactgca tttgaacaa tttgataag ttgagatgt gcgagcgga ccgcagacc aggtcacca gaagaacct acagcatga agtccacga ggtgtctgc caaagtgtg tgtgcgcgt ttgtggtgt cggtggttt ttattgtcg gcggttttg tgcttccgt ttatctggc cagtgattt tgaaatatt taatcgaga gtaacatgg tgttaatgc cgacttcgc acgcttttg atatctgga ggaagtttc cgacggcgt tccccatcg atttctact tcttcaact ggacgaatc cagaggacg tgtacaaca gttcggtga aaatgaaat ttcgggaaa tggggcctt atcggtact cggagacga aagaaaagg ccgatatcg tttggaata aaaacggga cggtttcgt tcagacact tgaagtttt ggtacgaaa atcgggaga aaggaaata tcaacgacg aaatcacca ctgtaaatc cagtcgctt tatcagctg cctactccg cgcgcacgt ggggttact taatccgcc aaggcctct ccttatccc tgtccagca tgtccccaa ctgtccaca tcactaggt ccgtgtcag agatgcttt tcaccggct acaaggacc ctctgatca cccttgccc gctccttgc cctttctat cgggctctt tgccacctt gggacaaat tcggttggt tctacaccc ggaacggat cagcgcatt acgaaggca tttttaaca tgggaactg gaataaact ccacttttg gtagactgt acagttgga attattgga cacaaacgc cttattacg agggctctt gtgccatgg ttaatggtt cagcggggg aatttttca ccaagcttg ataataaat cgattagtt ttttctcgc cggatttgt gccggacca tgacgctca ggtactccg ggcagagcg tcgtgaata atatactag ggaacaagt acgtcgtcg acagttaca tgctcgata atggcacga ttttcccgg aaaaccgct gtttctgca acggcgaat gcgtgcctt cgggcctag tcaacgttt cgagctgtc gtttcggct caccatcat tcgcctcac tgccgcatt tctaccaag cagatgcct actataccg actcaatcg aaggcgttc gcccggaaa aatccaagc acgagtttt tcctcaccc tggagccaa ccaccggaa ttccgttgg aagtcagcg cccggctcc aaattaatc tactaatgc agcccgatt ccgggatat cgttgtaca aaggcgtcc ctaaagttt ttgtgccca ttctatggt tcgagcaga aaattcgga ttccggacg gtgaagctt tcaagttga gattactcc tcaatcttc cggtgatct gcaccgctt tcgggatta tcctcgtgt ttgctggcg tgattgttg tgtcgttac ttgtttata aaatatgca cgatgaatt tgtgcggca agagaaaag agaagttgt tgtacgccg accaaagta ttccactca aaatggacc ggtcgctcg aaattgtta aaaagattt gaattgttg taaccgaat tgttattta taatagata attattatt gtaatttgt tatgttaat aaaaatttg tttctatt
- >TC012756 RB
ccgatcgaa aatgcgacg agaaaaata ttgatttta aaacgtaac aaacgtgcg gtaaataaa tattggcgc aaaatgggc aaaacgctc tttcggtta cgtaattgt tattgagat gaaagtcat cattttttc taatagtgt gcactttaa atacaatgc aagtttgcc gcaagtgca atgcgcgat gcaactgca tttgaacaa tttgataag ttgagatgt gcgagcgga ccgcagacc agcgcaatt cgctggtag taaacatag ccttgagac cactcattt ccaccttga taatgaact agataaaac cataaaaac gtaaaaatc acagtgata acctatcaa tagattgtg tgtgctggt ccaaaaatc ggtcaatct catgtgagt taatcaaat catctcgta taatcgcgt gcacgccca catgtcatc ttttccgtt caatattca aattttccg tccgagcgt attagccac cctataatc gtggtgttt tcctcaaag cggtgcagc cactggccg ataccttaa taatttaat ccaatttcc tccggtata aagccacca tcaggcaca ttgcatcag caagtcggt aaaaagctt ccaattagt ccgaatttg tgatttcgc gtaaagtga attaacgag gggtcaaat ggtgcttca cctccgact taataacaa gcactcgct cggatcttt attaggttg ttgttctaa accatgaag tgcagctaa atgccagcc atggtcacc agaagaacc tacagcatg aagtccacg aggtgtctg ccaaagtgt gtgtgcgcg tttgtggtg tcggtggtt tttattgtc ggcggtttt gtgcttccg tttatctgg ccagtgatt ttgaaatat ttaatcgag agtaacatg gtgttaatg ccgacttcg cacgctttt gatatctgg aggaagttt ccgacggcg ttccccatc gatttctac ttcttcaac tggacgaat ccagaggac gtgtacaac agttcggtg aaaatgaaa tttcgggaa atggggcct tatcggtac tcggagacg aaagaaaag gccgatatc gtttggaat aaaaacggg acggtttcg ttcagacac ttgaagttt tggtacgaa aatcgggag aaaggaaat atcaacgac gaaatcacc actgtaaat ccagtcgct ttatcagct gcctactcc gcgcgcacg tggggttac ttaatccgc caaggcctc tccttatcc ctgtccagc atgtcccca actgtccac atcactagg tccgtgtca gagatgctt ttcaccggc tacaaggac cctctgatc acccttgcc cgctccttg ccctttcta tcgggctct ttgccacct tgggacaaa ttcggttgg ttctacacc cggaacgga tcagcgcat tacgaaggc atttttaac atgggaact ggaataaac tccactttt ggtagactg tacagttgg aattattgg acacaaacg ccttattac gagggctct tgtgccatg gttaatggt tcagcgggg gaatttttc accaagctt gataataaa tcgattagt tttttctcg ccggatttg tgccggacc atgacgctc aggtactcc gggcagagc gtcgtgaat aatatacta gggaacaag tacgtcgtc gacagttac atgctcgat aatggcacg attttcccg gaaaaccgc tgtttctgc aacggcgaa tgcgtgcct tcgggccta gtcaacgtt tcgagctgt cgtttcggc tcaccatca ttcgcctca ctgccgcat ttctaccaa gcagatgcc tactatacc gactcaatc gaaggcgtt cgcccggaa aaatccaag cacgagttt ttcctcacc ctggagcca accaccgga attccgttg gaagtcagc gcccggctc caaattaat ctactaatg cagcccgat tccgggata tcgttgtac aaaggcgtc cctaaagtt tttgtgccc attctatgg ttcgagcag aaaattcgg attccggac ggtgaagct ttcaagttg agattactc ctcaatctt ccggtgatc tgcaccgct ttcgggatt atcctcgtg tttgctggc gtgattgtt gtgtcgtta cttgtttat aaaatatgc acgatgaat ttgtgcggc aagagaaaa gagaagttg ttgtacgcc gaccaaagt attccactc aaaatggac cggtcgctc gaaattgtt aaaaagatt tgaattgtt gtaaccgaa ttgttattt ataatagat aattattat tgtaatttg ttatgttaa taaaaattt gtttctatt
