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Gene information
General information
TC012752 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 14241805..14242979
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012752
    iBeetle screen iB_05309

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Pupal morphology
    beetle stock: bad (increased lethality),
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    head shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012752 RA
      MRKALTRKF SGSKLGRVF HRQKSGDVS KEGQMSPDS 
      FKEIESLKE EIDSLKTAL NTLKIDLEN RDGKLGKLA 
      REKEKLSLS LAKMKRSND NLARQLEDE RDFYYKEKE 
      TYCHEMNEF KKLKKALSN STLGEPPDH KEVEKLRKT 
      LEQTLQTNY NLSVKFLRM KETKNQLAI LLQKAEDNH 
      RRVVSDLKT KIETLTAEL KQILDMKFA APLSPSNKK 
      YLQLVNQNS SLVYENLHL QMQVDHLSV KRRHKSEGT 
      IVKIYEREK RSDIPTVTI LNEKEIGLR SERKIQLPV 
      INDSKLEVF QVSDLDSAE SVKVKKRSR SAPRVIQSV 
      NK
    CDS sequence
    • >TC012752 RA
      atgagaaaa gccctaact agaaaattt tccgggtca 
      aagcttgga cgcgttttc catcgtcag aaatccggt 
      gatgtttcg aaagaaggc cagatgagt cctgactct 
      tttaaagaa atcgagtct ttaaaggag gaaatcgat 
      agtttgaaa actgcacta aatacactc aaaattgac 
      ttggaaaat agggacggc aagctgggg aaactagca 
      agggaaaag gaaaaactg tcgctgagt ctcgctaaa 
      atgaaacgc tcgaacgac aacctcgcg cgccagctc 
      gaagacgag cgagatttc tactacaag gagaaggaa 
      acctactgc cacgaaatg aacgagttc aaaaagctc 
      aaaaaggcg ctaagtaac tcgaccctg ggcgagcca 
      cccgaccac aaagaagtg gaaaagctg cgaaagacg 
      ctcgagcaa acgctccaa accaactat aatctaagt 
      gttaaattc ctacgcatg aaggagacc aaaaaccag 
      ctcgcgatc ttgctccag aaagcggaa gacaaccac 
      cgacgggta gtcagcgac cttaaaacc aaaatcgag 
      accctcacc gccgaactg aaacaaatc ctcgacatg 
      aaattcgca gccccgttg tccccaagc aacaaaaag 
      tacctacaa ctcgtgaac cagaacagc tcgctggtt 
      tacgaaaac ttgcacctg cagatgcag gtcgatcat 
      ttgagcgtg aaacggcgg cataaaagc gagggcacg 
      atcgtgaaa atttacgaa agggagaag cgctccgat 
      attccaact gtgacaatt ttaaacgag aaggaaatc 
      ggtctgagg tccgaacgc aagatccag ctcccggtc 
      atcaacgac tccaaactt gaggtcttc caagtctcg 
      gacttggac agtgcggag tcggtgaaa gtcaagaag 
      cggagccgc tcagcaccg agagtcatc caaagtgtt 
      aataaatag
    mRNA sequence
    • >TC012752 RA
      ggtttcccg aaaatttac atgagaaaa gccctaact 
      agaaaattt tccgggtca aagcttgga cgcgttttc 
      catcgtcag aaatccggt gatgtttcg aaagaaggc 
      cagatgagt cctgactct tttaaagaa atcgagtct 
      ttaaaggag gaaatcgat agtttgaaa actgcacta 
      aatacactc aaaattgac ttggaaaat agggacggc 
      aagctgggg aaactagca agggaaaag gaaaaactg 
      tcgctgagt ctcgctaaa atgaaacgc tcgaacgac 
      aacctcgcg cgccagctc gaagacgag cgagatttc 
      tactacaag gagaaggaa acctactgc cacgaaatg 
      aacgagttc aaaaagctc aaaaaggcg ctaagtaac 
      tcgaccctg ggcgagcca cccgaccac aaagaagtg 
      gaaaagctg cgaaagacg ctcgagcaa acgctccaa 
      accaactat aatctaagt gttaaattc ctacgcatg 
      aaggagacc aaaaaccag ctcgcgatc ttgctccag 
      aaagcggaa gacaaccac cgacgggta gtcagcgac 
      cttaaaacc aaaatcgag accctcacc gccgaactg 
      aaacaaatc ctcgacatg aaattcgca gccccgttg 
      tccccaagc aacaaaaag tacctacaa ctcgtgaac 
      cagaacagc tcgctggtt tacgaaaac ttgcacctg 
      cagatgcag gtcgatcat ttgagcgtg aaacggcgg 
      cataaaagc gagggcacg atcgtgaaa atttacgaa 
      agggagaag cgctccgat attccaact gtgacaatt 
      ttaaacgag aaggaaatc ggtctgagg tccgaacgc 
      aagatccag ctcccggtc atcaacgac tccaaactt 
      gaggtcttc caagtctcg gacttggac agtgcggag 
      tcggtgaaa gtcaagaag cggagccgc tcagcaccg 
      agagtcatc caaagtgtt aataaatag tacaaagag 
      cgtggcatt ttgaatttc c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY