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Gene information
General information
TC012748 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 14176634..14180807
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012748
    iBeetle screen iB_05308

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants strongly fragmented (cuticle crumbs) - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012748 RA
      MEETKTEIK SKISFSVES LLSKKEDEE 
      DEHRLSDRD DDDENITVD DDDTDGRES 
      LSPSSAHTV LIPQPLHPS VPRLGHPQW 
      PFAWGSLMR SSSPQTSAP TGPPIVRCA 
      LRKHKPNRK PRTPFTTQQ LLALEKKFR 
      DKQYLSIAE RAEFSSSLR LTETQVKIW 
      FQNRRAKAK RLQEAELEK LRLSARPLL 
      PPSFGIFPG GAPPLVSPF LAAMAHRPP 
      HFAFPGAPG HILNIPQ
    CDS sequence
    • >TC012748 RA
      atggaagaa acgaaaaca gaaatcaaa tcgaagatc 
      tccttcagc gtggagtcg ctgctgtcg aagaaggag 
      gacgaggag gacgagcac aggctgagc gatcgggac 
      gacgacgac gagaatatc acggtggac gacgacgac 
      accgacggc agggagagt ctgagtcct agttccgct 
      cacaccgtt ttaataccg cagccgctg catccctcc 
      gtgcccagg ctggggcac ccgcagtgg ccattcgcc 
      tgggggtct ctcatgcgc tcctcctcc ccgcaaacc 
      agcgccccc acgggtccc cccatcgtg cgctgcgcc 
      ctccggaag cacaaaccc aaccgaaag ccgcgcacc 
      cccttcaca acgcaacag ctcctagcc cttgaaaaa 
      aaattccgg gacaagcag tacttgagc atagccgag 
      cgggcagag ttttccagt tcgctgcgg ctgacggag 
      acccaagtc aagatctgg ttccagaac cggagggcg 
      aaggccaag cggctccag gaggccgag ctggagaag 
      ctgcggctg tcggcgagg ccgcttttg ccccccagt 
      ttcgggatt ttcccgggc ggggcgccg cccctagtg 
      agccccttt ctggcggcc atggcgcac cggcccccg 
      catttcgcc tttccgggg gcgccgggg cacatcctc 
      aacataccc caatga
    mRNA sequence
    • >TC012748 RA
      tcagtccgc tgcgagcaa cagtccgac gacgtctcg 
      agtgagttt tgatgagtg agtgccaaa gatggaaga 
      aacgaaaac agaaatcaa atcgaagat ctccttcag 
      cgtggagtc gctgctgtc gaagaagga ggacgagga 
      ggacgagca caggctgag cgatcggga cgacgacga 
      cgagaatat cacggtgga cgacgacga caccgacgg 
      cagggagag tctgagtcc tagttccgc tcacaccgt 
      tttaatacc gcagccgct gcatccctc cgtgcccag 
      gctggggca cccgcagtg gccattcgc ctgggggtc 
      tctcatgcg ctcctcctc cccgcaaac cagcgcccc 
      cacgggtcc ccccatcgt gcgctgcgc cctccggaa 
      gcacaaacc caaccgaaa gccgcgcac ccccttcac 
      aacgcaaca gctcctagc ccttgaaaa aaaattccg 
      ggacaagca gtacttgag catagccga gcgggcaga 
      gttttccag ttcgctgcg gctgacgga gacccaagt 
      caagatctg gttccagaa ccggagggc gaaggccaa 
      gcggctcca ggaggccga gctggagaa gctgcggct 
      gtcggcgag gccgctttt gccccccag tttcgggat 
      tttcccggg cggggcgcc gcccctagt gagcccctt 
      tctggcggc catggcgca ccggccccc gcatttcgc 
      ctttccggg ggcgccggg gcacatcct caacatacc 
      ccaatgaca gtgagtggt tgtggcgga gtgcaatct 
      ttccataag actgaagag acttttcct gttttgtat 
      aaatgtgtt tgtttatga tgtacaaag gtttattta 
      atttcgttg tattataat gtacatagg atgctatta 
      aatgtttgt ttttaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY