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Gene information
General information
TC012737 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 13906661..13908337
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012737
    iBeetle screen iB_05307

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Pupal morphology
    beetle stock: bad (increased lethality),
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012737 RA
      MNATQSIYT FLPACSSPA KPFADLGRP TKRGIKKCP 
      KCGIYNGTR GMICKNKHC DAVFKDYAD KRKLSLDAV 
      RLVGIVRQV YSVRVRDRG PDHRGFVQL PLLNPEQDA 
      LCFVDSCQR LFDDSILKC HELEHSETA LLCVHIDAA 
      IKSQSMAAP IEVQKEVLA SLKVSKEIK AKLWQLATE 
      KEGPLVQRV SRTVMAVKC QVSPKHPLG YLHFTFGCT 
      KGREIYDKY YCSCTEFMV SGIISKASN VRLKCVHYY 
      ACICALASD PKCAQEFSH FINYELENG SEGPQVESV 
      KSTPKPDRV PKLKVVERV TKRPKFKKL VPNVFPVEI 
      KVLESVEQV APSGGISWG FIEWLSYVT ESINQTMHF 
      ENYGNLNTL VFCIPEAFF QEFTKRIPS IYRENEVFP 
      AFNYRIMNI LHLKEIFDT PDVKLKISK RFIHDNQKG 
      YIEYDVDAN ISCQSSFIF FLNVGQTTV DESDNTNNP 
      FLIEWIPGV TNATKIGQL KLQYKYGRK STT
    CDS sequence
    • >TC012737 RA
      atgaatgcg acccagtcc atttacacc ttcctccct 
      gcgtgctcc tctccggcc aaacccttc gccgatttg 
      ggccggccc acgaaacgg ggcataaaa aaatgcccc 
      aagtgcggc atctacaac ggcacccgg ggcatgatc 
      tgcaagaac aagcactgc gacgccgtg ttcaaagac 
      tacgccgac aagcgcaag ttgagtctg gacgccgtg 
      cgcctcgtc gggatcgtg cgccaggtg tactcggtg 
      cgggtgcgc gaccggggg cccgaccac cggggcttc 
      gtccagctg ccgctgctg aaccccgaa caggacgcc 
      ctgtgtttc gttgattcg tgccagagg ctgttcgac 
      gactcgatt ctcaagtgc catgagctg gagcacagc 
      gagaccgcg ctgctgtgc gtccacatc gacgcggcg 
      attaagagc cagagcatg gcagccccc attgaggtc 
      caaaaggag gttttggcc tcgttgaag gtttcgaaa 
      gaaattaaa gcgaagctg tggcagttg gccacggag 
      aaagagggg cctctggtg cagagagtc tcgcggacc 
      gtgatggcg gtcaagtgc caagtgagc cccaaacac 
      cccctgggg tacctccac tttaccttc gggtgcacg 
      aaaggccgc gagatttac gacaagtat tactgcagt 
      tgtaccgaa tttatggtt tcagggatt ataagcaag 
      gcgagcaat gtccgactc aaatgtgtg cactattat 
      gcgtgcatt tgtgccctt gccagcgac ccgaaatgt 
      gcgcaagag tttagtcat ttcattaat tacgaattg 
      gagaacggc tccgaaggg ccacaagtt gaaagtgtt 
      aaaagcacg cctaaacct gacagggtg ccgaaactg 
      aaggtggtt gagcgagtg acgaaacgt ccaaaattc 
      aaaaaactg gtccctaat gtgtttccg gttgaaatc 
      aaagtgctg gaaagtgtg gaacaagtc gccccaagt 
      gggggaatt tcgtggggg tttatcgaa tggttgtct 
      tatgtgact gaatccata aaccaaacc atgcacttc 
      gagaattac ggcaattta aacactttg gttttttgc 
      atccctgag gcatttttt caagaattt actaaacga 
      attccaagt atctatcgg gaaaacgaa gtttttcct 
      gcctttaac taccgaatt atgaacatt ttgcatctc 
      aaagagata tttgatact ccagatgtc aagttgaaa 
      atttccaag agatttatt catgacaat caaaagggt 
      tacattgag tacgatgtt gatgcaaac atcagttgt 
      cagtcaagt tttattttt ttcctaaat gttggacaa 
      accacggtt gacgagagt gacaacacg aataatccc 
      ttcttgatc gagtggatc cccggagtc accaatgcc 
      acaaaaatt ggacagttg aagttgcag tataaatat 
      gggcgcaaa agtactact tga
    mRNA sequence
    • >TC012737 RA
      cctcagtgc tatgaatgc gacccagtc catttacac 
      cttcctccc tgcgtgctc ctctccggc caaaccctt 
      cgccgattt gggccggcc cacgaaacg gggcataaa 
      aaaatgccc caagtgcgg catctacaa cggcacccg 
      gggcatgat ctgcaagaa caagcactg cgacgccgt 
      gttcaaaga ctacgccga caagcgcaa gttgagtct 
      ggacgccgt gcgcctcgt cgggatcgt gcgccaggt 
      gtactcggt gcgggtgcg cgaccgggg gcccgacca 
      ccggggctt cgtccagct gccgctgct gaaccccga 
      acaggacgc cctgtgttt cgttgattc gtgccagag 
      gctgttcga cgactcgat tctcaagtg ccatgagct 
      ggagcacag cgagaccgc gctgctgtg cgtccacat 
      cgacgcggc gattaagag ccagagcat ggcagcccc 
      cattgaggt ccaaaagga ggttttggc ctcgttgaa 
      ggtttcgaa agaaattaa agcgaagct gtggcagtt 
      ggccacgga gaaagaggg gcctctggt gcagagagt 
      ctcgcggac cgtgatggc ggtcaagtg ccaagtgag 
      ccccaaaca ccccctggg gtacctcca ctttacctt 
      cgggtgcac gaaaggccg cgagattta cgacaagta 
      ttactgcag ttgtaccga atttatggt ttcagggat 
      tataagcaa ggcgagcaa tgtccgact caaatgtgt 
      gcactatta tgcgtgcat ttgtgccct tgccagcga 
      cccgaaatg tgcgcaaga gtttagtca tttcattaa 
      ttacgaatt ggagaacgg ctccgaagg gccacaagt 
      tgaaagtgt taaaagcac gcctaaacc tgacagggt 
      gccgaaact gaaggtggt tgagcgagt gacgaaacg 
      tccaaaatt caaaaaact ggtccctaa tgtgtttcc 
      ggttgaaat caaagtgct ggaaagtgt ggaacaagt 
      cgccccaag tgggggaat ttcgtgggg gtttatcga 
      atggttgtc ttatgtgac tgaatccat aaaccaaac 
      catgcactt cgagaatta cggcaattt aaacacttt 
      ggttttttg catccctga ggcattttt tcaagaatt 
      tactaaacg aattccaag tatctatcg ggaaaacga 
      agtttttcc tgcctttaa ctaccgaat tatgaacat 
      tttgcatct caaagagat atttgatac tccagatgt 
      caagttgaa aatttccaa gagatttat tcatgacaa 
      tcaaaaggg ttacattga gtacgatgt tgatgcaaa 
      catcagttg tcagtcaag ttttatttt tttcctaaa 
      tgttggaca aaccacggt tgacgagag tgacaacac 
      gaataatcc cttcttgat cgagtggat ccccggagt 
      caccaatgc cacaaaaat tggacagtt gaagttgca 
      gtataaata tgggcgcaa aagtactac ttgaatata 
      ataataaaa cactgaaag actg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY