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Gene information
General information
TC012735 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 13901022..13903694
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012735
    iBeetle screen iB_05306

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Pupal morphology
    beetle stock: bad (increased lethality),
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 6 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 6 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012735 RA
      MKPRKKPEK NAPNPFEIH INKQKMTVL GQKNKNDRG 
      LPGVSRAKA LKKRKETLL QEYKLQNKS NKFTDRRIG 
      ERSRDLTSE DKLMARFTA LRKKAHKKS IFNLADDEV 
      LTHKGQTLA EIEKFDDPR SDNEDSDED KSGKLDSAF 
      VGDAHFGGG VLRKTGKEG AKTHRELID ELIAESKKR 
      KAEKQRTKE ATLELTEKL DTEWKDLLP LVSRAQKGE 
      PDKGAVDDY DKVMRELRF EARGTVSDR LKSEDEVAQ 
      EEKQRLEEL EEKRRERMR GFEPKTQKH RSADDLDDD 
      VYEADPEYM LSYNTEGEA NVEIGAEIN GKTVGGEES 
      DEEEECEEE EEEQSDDDL ADLKAETSD ESDEETEAK 
      ATKTDVVAK TDETSADLP FTFALPDSY EALQQLLDD 
      KSSRQQATI LERMVKCNH PSLAEKNKN GLGTLFVYL 
      LQYLNDCAS EKSAARLFE IYKSLAPHI FDLAQLNKE 
      NTHSSMLEV IKEKHDEFK KRPKDFPGL ELLVFFKLV 
      SLLFTTSDF RHQVVTPCF VFIEQILNK CKIKSGKDI 
      AYGIFLVTL VLEYTSLSK RVLPTAINY LGGILHMAI 
      PKCGVKLIK VNPPFKPTT SSLVLVEKS CEDCDFKME 
      ASDLERDEI NDSFKIRAI YTVLRLVQD FDNNLVSLP 
      SNVEIFNDI RAYLELLPT RNYPKKVAD LFESVSQQL 
      KNRKVERKL EYIVLAAKR PKALRLYEP KIVEVFDGK 
      KRKIQSREK AERDKLLHK LKKETKGAL REIRRDKAF 
      LGRVKIHQR IQSDNERKE KVKRIYAEA SIQQSELNS 
      LDRKKKRK
    CDS sequence
    • >TC012735 RA
      atgaagccg cgcaaaaaa cccgaaaaa aacgccccc 
      aacccgttc gaaatccac atcaacaag caaaaaatg 
      accgtcctg ggccagaaa aacaaaaac gatcggggg 
      ctgccggga gtctcacgc gcgaaagcg ctcaaaaaa 
      cgcaaagaa accctcctt caggagtac aaactgcaa 
      aacaaatcg aacaagttt acggaccgg aggatcggc 
      gagcggagc cgcgacctg acctctgaa gacaagctg 
      atggcccgg ttcaccgcc cttcggaaa aaagcgcac 
      aaaaagtcg atttttaac ctggccgac gacgaagtt 
      ctgacccac aaggggcaa actcttgcc gaaatagag 
      aaattcgac gacccgagg tcggataac gaggactcg 
      gatgaggac aagtcgggt aagctggac tcagcgttt 
      gtgggggac gcgcacttc gggggcggc gttttgcgc 
      aaaacgggc aaagaaggg gcgaagacg caccgagaa 
      ctgatcgac gagttgatt gccgagtcg aagaaacgc 
      aaagctgag aagcaacgc accaaggag gcgacgctc 
      gagctcact gagaaattg gacacggag tggaaggac 
      ttgctgccg ctggtgagc agggcccag aagggggag 
      cccgacaag ggggccgtc gacgactac gataaggtc 
      atgcgtgag ttgaggttt gaggcgcgc gggacggtc 
      tccgaccgg ttgaagagt gaggacgag gtggctcag 
      gaggaaaaa cagcggttg gaggagttg gaggagaag 
      cggcgggag cgaatgcgg gggtttgag cccaagact 
      cagaagcac cgcagtgct gacgattta gacgatgat 
      gtttacgag gcggatcct gagtacatg ctcagctac 
      aacacggag ggggaggca aatgtggaa attggggct 
      gagataaac gggaaaact gttggtggg gaagaaagt 
      gacgaagaa gaagagtgt gaagaagag gaggaagaa 
      cagtccgat gatgatttg gccgatctt aaggccgaa 
      acgagcgac gaaagtgac gaggaaacc gaagccaag 
      gccacaaag actgatgtt gttgcaaaa accgacgaa 
      acgtcggcc gacctgccg ttcacgttc gcactgcct 
      gactcctac gaggccctc cagcaactc ctcgacgac 
      aaatcgagt cgccaacaa gccacgatt cttgaaaga 
      atggtcaag tgcaatcac ccaagtctc gccgaaaaa 
      aacaaaaac ggacttggc acactgttt gtctattta 
      ttgcagtat ttgaacgat tgtgcgtcg gaaaaaagc 
      gctgcccgt ttgttcgaa atttacaaa agtttggct 
      ccgcacatt tttgatttg gctcagcta aataaagaa 
      aatacccac agttcgatg cttgaagtg attaaggaa 
      aaacacgac gagtttaaa aaacgaccg aaggatttt 
      cccggcttg gagttgttg gtttttttc aagctggtc 
      agtttgttg ttcacaacg tccgatttt cggcatcag 
      gttgtgacc ccgtgtttt gtttttatt gaacaaatt 
      ttgaataag tgtaaaata aaaagtggc aaagatatc 
      gcgtacggg attttttta gtgactttg gttctagag 
      tatacttca ctatcgaag cgggtttta ccgactgcg 
      attaattat ctcggaggg atcttgcat atggcgatc 
      cccaagtgt ggggtgaaa ctaatcaaa gtcaatcct 
      cccttcaag cccacgaca agttctcta gttcttgtt 
      gaaaagtct tgcgaggat tgtgatttc aaaatggaa 
      gcttctgat ttagaaagg gacgaaatt aacgattct 
      ttcaaaatc agagcaatt tatactgtt ttaaggcta 
      gttcaagac tttgacaat aatctagtt tctctgccc 
      tcaaatgtt gagattttt aacgatatt cgagcgtat 
      ttggagtta ctaccgacg cgaaattac ccgaaaaag 
      gtcgccgat ttgtttgaa agcgtttca caacaatta 
      aaaaatcgc aaagttgag cgaaaatta gaatacatt 
      gttttggca gcaaaacga ccgaaagcg ctacgctta 
      tatgaacct aaaattgtg gaagtattt gatggtaaa 
      aaacgtaaa attcaatca agggaaaaa gccgagcgt 
      gacaaattg ttacataaa cttaagaaa gaaacgaag 
      ggtgcattg cgtgaaata cggagagat aaggcgttt 
      ctgggacgt gtcaaaatt catcaacgc atacaaagt 
      gataatgag cgcaaggaa aaagttaaa cgaatttat 
      gccgaagct tcgatccag caaagcgaa ttgaacagt 
      ttagacagg aagaagaaa cgcaagtga
    mRNA sequence
    • >TC012735 RA
      aaagcataa cctcacaaa aacacgtgt tttgtcacc 
      atgaagccg cgcaaaaaa cccgaaaaa aacgccccc 
      aacccgttc gaaatccac atcaacaag caaaaaatg 
      accgtcctg ggccagaaa aacaaaaac gatcggggg 
      ctgccggga gtctcacgc gcgaaagcg ctcaaaaaa 
      cgcaaagaa accctcctt caggagtac aaactgcaa 
      aacaaatcg aacaagttt acggaccgg aggatcggc 
      gagcggagc cgcgacctg acctctgaa gacaagctg 
      atggcccgg ttcaccgcc cttcggaaa aaagcgcac 
      aaaaagtcg atttttaac ctggccgac gacgaagtt 
      ctgacccac aaggggcaa actcttgcc gaaatagag 
      aaattcgac gacccgagg tcggataac gaggactcg 
      gatgaggac aagtcgggt aagctggac tcagcgttt 
      gtgggggac gcgcacttc gggggcggc gttttgcgc 
      aaaacgggc aaagaaggg gcgaagacg caccgagaa 
      ctgatcgac gagttgatt gccgagtcg aagaaacgc 
      aaagctgag aagcaacgc accaaggag gcgacgctc 
      gagctcact gagaaattg gacacggag tggaaggac 
      ttgctgccg ctggtgagc agggcccag aagggggag 
      cccgacaag ggggccgtc gacgactac gataaggtc 
      atgcgtgag ttgaggttt gaggcgcgc gggacggtc 
      tccgaccgg ttgaagagt gaggacgag gtggctcag 
      gaggaaaaa cagcggttg gaggagttg gaggagaag 
      cggcgggag cgaatgcgg gggtttgag cccaagact 
      cagaagcac cgcagtgct gacgattta gacgatgat 
      gtttacgag gcggatcct gagtacatg ctcagctac 
      aacacggag ggggaggca aatgtggaa attggggct 
      gagataaac gggaaaact gttggtggg gaagaaagt 
      gacgaagaa gaagagtgt gaagaagag gaggaagaa 
      cagtccgat gatgatttg gccgatctt aaggccgaa 
      acgagcgac gaaagtgac gaggaaacc gaagccaag 
      gccacaaag actgatgtt gttgcaaaa accgacgaa 
      acgtcggcc gacctgccg ttcacgttc gcactgcct 
      gactcctac gaggccctc cagcaactc ctcgacgac 
      aaatcgagt cgccaacaa gccacgatt cttgaaaga 
      atggtcaag tgcaatcac ccaagtctc gccgaaaaa 
      aacaaaaac ggacttggc acactgttt gtctattta 
      ttgcagtat ttgaacgat tgtgcgtcg gaaaaaagc 
      gctgcccgt ttgttcgaa atttacaaa agtttggct 
      ccgcacatt tttgatttg gctcagcta aataaagaa 
      aatacccac agttcgatg cttgaagtg attaaggaa 
      aaacacgac gagtttaaa aaacgaccg aaggatttt 
      cccggcttg gagttgttg gtttttttc aagctggtc 
      agtttgttg ttcacaacg tccgatttt cggcatcag 
      gttgtgacc ccgtgtttt gtttttatt gaacaaatt 
      ttgaataag tgtaaaata aaaagtggc aaagatatc 
      gcgtacggg attttttta gtgactttg gttctagag 
      tatacttca ctatcgaag cgggtttta ccgactgcg 
      attaattat ctcggaggg atcttgcat atggcgatc 
      cccaagtgt ggggtgaaa ctaatcaaa gtcaatcct 
      cccttcaag cccacgaca agttctcta gttcttgtt 
      gaaaagtct tgcgaggat tgtgatttc aaaatggaa 
      gcttctgat ttagaaagg gacgaaatt aacgattct 
      ttcaaaatc agagcaatt tatactgtt ttaaggcta 
      gttcaagac tttgacaat aatctagtt tctctgccc 
      tcaaatgtt gagattttt aacgatatt cgagcgtat 
      ttggagtta ctaccgacg cgaaattac ccgaaaaag 
      gtcgccgat ttgtttgaa agcgtttca caacaatta 
      aaaaatcgc aaagttgag cgaaaatta gaatacatt 
      gttttggca gcaaaacga ccgaaagcg ctacgctta 
      tatgaacct aaaattgtg gaagtattt gatggtaaa 
      aaacgtaaa attcaatca agggaaaaa gccgagcgt 
      gacaaattg ttacataaa cttaagaaa gaaacgaag 
      ggtgcattg cgtgaaata cggagagat aaggcgttt 
      ctgggacgt gtcaaaatt catcaacgc atacaaagt 
      gataatgag cgcaaggaa aaagttaaa cgaatttat 
      gccgaagct tcgatccag caaagcgaa ttgaacagt 
      ttagacagg aagaagaaa cgcaagtga ggcttttag 
      cccccttta gaattttgt aaataaaag tattgtttt 
      ttaaagtga gtttttatt tcgtttaaa caataaaaa 
      ttactatat ata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY