Status
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Gene information
General information
TC012731 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 13857612..13862855
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012731
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05305

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    mature egg deposition 2 number 2nd lateral oviduct: number 1st lateral oviduct: blocked - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 3

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    Fertility
    egg production: number no/almost no eggs -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012731 RA
      MLRIVGRFP SKNLQTKSR RILEFKLYK SVRCYSKDV 
      CPPPPPKRK DKSSSGALY TLGALALVG GATIGYAKY 
      DPDFRKTLG EYVPWTDDV IKFVFQEEH SLLDSASGV 
      YEDVKKSVS GLLTQEQRK KKEDVKLEQ KQYKAPQPV 
      IPALEDTKV PQEGYTEIR IEQKDKRDV EIGGEAKVP 
      PGEVSVPES HPSNLAELE AKICDAATE AVNFYNKAV 
      YDLRVYNGE ISQILDQSV DQVKPPQWE ALKAKTKAK 
      NESVRLAEE KAAQAAQNV SKLKTLLTK TSAATKEMV 
      NINIDKVQE DIDAAKAQY EIERKLGSI TDKYWEKVQ 
      KARHHFCEE LESLFPAID IYNKKLEVN EADLDLFIL 
      HAYSVVLYY QKELAKLET VAHEKLQSA LDAARRGGF 
      EPLGKAQIC EAIEEEKRK LTLCFQKQC LKLRKEAEH 
      ELREQLKRQ SQVFADHLD EAVTTRALE IERVLSRKF 
      DEEMEKERF KHKSQLAAM VGRMKGLDH AFKARDAAD 
      KAAKQSQVL WAACQALLR AIKTGCPGI PWKDQLRPL 
      SPEITLVQK AAAKGDELV EAVINGIPK EAKERGVFP 
      EDALRERFL KVEDVARRV ALVPEEGGA LPLHILSYI 
      QAFFLIKNP SPIPQAELN DEAVDFSQL STNEILQRA 
      RYWLDRGDF AQTLKYMNL LQGAPRSIA RQWMNETRI 
      LLETQQAAN TLMAHAASS GLLHL
    CDS sequence
    • >TC012731 RA
      atgttaaga attgtagga aggttcccg tcgaaaaat 
      ctccaaacg aaatcccgt cgaattttg gaattcaaa 
      ttgtataag agtgtgcga tgttactcg aaggacgtg 
      tgtccgccc ccgcccccc aaacgaaaa gacaagtcc 
      agttccggt gcgctgtac accttgggg gcactcgct 
      ttggtcggt ggtgccaca atcggttac gctaaatac 
      gacccagac ttccgcaag actttgggc gagtacgtc 
      ccctggacc gacgacgtg atcaagttt gtgttccaa 
      gaggagcac agtctactt gactcggct tcgggggtc 
      tacgaggac gtgaagaag tcggtctcg gggctgctg 
      acgcaagaa cagcgcaag aagaaagag gacgtgaaa 
      ttagaacag aagcaatac aaagccccc cagccagtg 
      ataccggca cttgaagac accaaggtc ccccaggag 
      gggtacacg gagatccgc atcgagcag aaggacaag 
      cgcgatgtc gaaattggt ggcgaggcc aaggtgccc 
      cctggcgag gtctccgtg cccgagtcg caccccagc 
      aacttggcc gaactcgag gcaaaaatc tgcgacgct 
      gccaccgaa gcagtcaat ttttacaac aaagctgtc 
      tacgacctc cgcgtttat aacggagaa ataagccag 
      attttggac cagtccgtg gaccaggtc aagcctcct 
      caatgggag gccctcaaa gccaaaacc aaggccaag 
      aacgagtcg gtgcgattg gccgaagag aaggcagct 
      caagcggcc caaaacgtc tcaaaatta aagactctc 
      ctaaccaaa acctccgca gccacgaaa gaaatggtc 
      aacattaac atcgataaa gtccaagag gacattgac 
      gcggccaaa gcgcagtac gaaatcgag agaaaattg 
      ggctcaatc accgataaa tactgggaa aaggtccaa 
      aaggctaga catcatttt tgcgaagag ttggaatcg 
      cttttcccc gcgatcgat atttacaat aaaaagttg 
      gaagtgaac gaggccgac ttggatttg tttatttta 
      cacgcgtat tccgttgtc ttgtattac caaaaagaa 
      ttggcaaaa ctggaaact gtggcgcac gagaaattg 
      cagtcggcg ttggatgcg gcacgaaga ggcggcttt 
      gaaccactt ggcaaggcc caaatttgc gaagccatc 
      gaagaggag aagcggaaa ttgactctg tgcttccag 
      aaacagtgt ctcaagttg cgaaaagag gccgagcac 
      gaattgcgc gaacaactc aaacgccaa tcgcaagtt 
      ttcgccgac cacttggat gaagccgtg accactcgt 
      gcgctcgaa atcgaaaga gtattgtcc cgaaaattc 
      gacgaagaa atggagaag gagcgtttc aagcataaa 
      tcgcagttg gcggcgatg gtgggccgg atgaaggga 
      ctcgaccat gcgtttaaa gcgcgcgat gcggccgat 
      aaagccgcc aaacaatcg caagtgttg tgggcggcg 
      tgtcaggcg ttgctaagg gcgattaag acgggctgt 
      ccggggatt ccctggaag gatcagttg cggccgtta 
      agtcccgag atcacgctt gtgcagaag gcggcagcc 
      aaaggggat gagttggtg gaggcggtc attaacggc 
      attccaaag gaggcgaag gagcgcggg gtgttcccg 
      gaggatgcg ctccgggag cgcttcctg aaggtggag 
      gatgtggcc aggagggtg gcgctagtg cccgaagag 
      ggcggggct ttgccgttg catatcttg tcgtatatc 
      caggcattt ttcctgatt aaaaatcct agtcctata 
      ccacaggcg gagctcaac gatgaagcc gttgatttt 
      agtcaattg agcacgaat gagatttta caaagggct 
      agatattgg ttagatcgt ggcgatttc gcccaaacg 
      cttaaatat atgaaccta ttacaaggc gctcccagg 
      tctatagcc aggcaatgg atgaacgaa acgaggatt 
      ttgttagag acacaacaa gctgccaac actttaatg 
      gcacatgcg gcgtctagt gggcttttg catctctaa
    mRNA sequence
    • >TC012731 RA
      gacaactta aaaaatacc gcataactt aaccttaga 
      attcgcaac caaaaacaa aaaaatcga gttaaagta 
      ataattagg ggtcaggtt gggcgtttt tgtgggttt 
      tgtgacatc ctggttgaa tctggggcg attttgggg 
      ccacttaca cgaattgag gactctcaa cattgataa 
      ttcgagtga tgtctgcac tgtcaagct aactgcaag 
      gagtatttt tgtgttgtc tgattttgc taaaaccgc 
      tcccaaagt ggctgcccc agaagatgt taagaattg 
      taggaaggt tcccgtcga aaaatctcc aaacgaaat 
      cccgtcgaa ttttggaat tcaaattgt ataagagtg 
      tgcgatgtt actcgaagg acgtgtgtc cgcccccgc 
      cccccaaac gaaaagaca agtccagtt ccggtgcgc 
      tgtacacct tgggggcac tcgctttgg tcggtggtg 
      ccacaatcg gttacgcta aatacgacc cagacttcc 
      gcaagactt tgggcgagt acgtcccct ggaccgacg 
      acgtgatca agtttgtgt tccaagagg agcacagtc 
      tacttgact cggcttcgg gggtctacg aggacgtga 
      agaagtcgg tctcggggc tgctgacgc aagaacagc 
      gcaagaaga aagaggacg tgaaattag aacagaagc 
      aatacaaag ccccccagc cagtgatac cggcacttg 
      aagacacca aggtccccc aggaggggt acacggaga 
      tccgcatcg agcagaagg acaagcgcg atgtcgaaa 
      ttggtggcg aggccaagg tgccccctg gcgaggtct 
      ccgtgcccg agtcgcacc ccagcaact tggccgaac 
      tcgaggcaa aaatctgcg acgctgcca ccgaagcag 
      tcaattttt acaacaaag ctgtctacg acctccgcg 
      tttataacg gagaaataa gccagattt tggaccagt 
      ccgtggacc aggtcaagc ctcctcaat gggaggccc 
      tcaaagcca aaaccaagg ccaagaacg agtcggtgc 
      gattggccg aagagaagg cagctcaag cggcccaaa 
      acgtctcaa aattaaaga ctctcctaa ccaaaacct 
      ccgcagcca cgaaagaaa tggtcaaca ttaacatcg 
      ataaagtcc aagaggaca ttgacgcgg ccaaagcgc 
      agtacgaaa tcgagagaa aattgggct caatcaccg 
      ataaatact gggaaaagg tccaaaagg ctagacatc 
      atttttgcg aagagttgg aatcgcttt tccccgcga 
      tcgatattt acaataaaa agttggaag tgaacgagg 
      ccgacttgg atttgttta ttttacacg cgtattccg 
      ttgtcttgt attaccaaa aagaattgg caaaactgg 
      aaactgtgg cgcacgaga aattgcagt cggcgttgg 
      atgcggcac gaagaggcg gctttgaac cacttggca 
      aggcccaaa tttgcgaag ccatcgaag aggagaagc 
      ggaaattga ctctgtgct tccagaaac agtgtctca 
      agttgcgaa aagaggccg agcacgaat tgcgcgaac 
      aactcaaac gccaatcgc aagttttcg ccgaccact 
      tggatgaag ccgtgacca ctcgtgcgc tcgaaatcg 
      aaagagtat tgtcccgaa aattcgacg aagaaatgg 
      agaaggagc gtttcaagc ataaatcgc agttggcgg 
      cgatggtgg gccggatga agggactcg accatgcgt 
      ttaaagcgc gcgatgcgg ccgataaag ccgccaaac 
      aatcgcaag tgttgtggg cggcgtgtc aggcgttgc 
      taagggcga ttaagacgg gctgtccgg ggattccct 
      ggaaggatc agttgcggc cgttaagtc ccgagatca 
      cgcttgtgc agaaggcgg cagccaaag gggatgagt 
      tggtggagg cggtcatta acggcattc caaaggagg 
      cgaaggagc gcggggtgt tcccggagg atgcgctcc 
      gggagcgct tcctgaagg tggaggatg tggccagga 
      gggtggcgc tagtgcccg aagagggcg gggctttgc 
      cgttgcata tcttgtcgt atatccagg catttttcc 
      tgattaaaa atcctagtc ctataccac aggcggagc 
      tcaacgatg aagccgttg attttagtc aattgagca 
      cgaatgaga ttttacaaa gggctagat attggttag 
      atcgtggcg atttcgccc aaacgctta aatatatga 
      acctattac aaggcgctc ccaggtcta tagccaggc 
      aatggatga acgaaacga ggattttgt tagagacac 
      aacaagctg ccaacactt taatggcac atgcggcgt 
      ctagtgggc ttttgcatc tctaatgtt gtataaatc 
      attaaatta aatagtaac aattacagt tttacttat 
      ttattattg tgctttcga tttattttt tcattgtta 
      tcctctgta tatttttaa accggtact tcatcaaga 
      gtgtacaat aaagacagt ccttgtta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY