General information | |||
TC012726 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 13712951..13719345 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC012726 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_05304 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%
head shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
Comment: The labium seems to be the most affected head part. |
head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC012726 RA
MDTIVQGPP GEGAQDLNL VDAAWSTQN SQEIVNNNS ILYIAVEYI KDEYKMESA VNLPPPENF ADFEQHVSP LDPNMSSVA RYSPVYSEP TTEYNPVVI HHLVQPNSS QSDINNLAP EPMPVLCNN DIREVVDNH FLQMVNSGN QLQVVPPNE QEVALLITD QETGISYSV RGQEFLVED EQLLNALSP DPLLDPHLL ALDENALKT ELGDEIINS TVNTAVNNY ITSLSAAVD NDGQYKMET RRQKQNEVE FEDQLLSKV YSIVDKPVP SRARATLPE SYLTLSKVD QALGVFAKK TIPRSTQFG PLTGILKPS TERLKNGPT SLQFFIEDE NNVVSQIDV SDENASNWM RFIRQATNY NEQNLLITQ EGNSLYFTT TRSILPKQE LKVGYSVPY ATRHKLSIL VPEEEKSWP CYECSETFA SSDELQKHL NIHDSDEEN KWKKKNLKN KKKIPKSET FQCNNCNEL FVQPGKVAL RQHLIEKHL LSGLDLIDQ YFSSVMNYK CDKCELVFN SEPLLKIHN YLHDSDSSD EQTNHVCPN CQKKFPTQR QLVTHVATH ALTKKPETE TFKCPVCHK MFAMRERLR RHMLVHGSD DSKPLQCKT CNKRFVNNS ALAGHIKTH LVGKKIFEC PICKENFDH VLKLKLHVP KHCENNTYS CPHCSKVFK KYSIIRKHI RAFHCDQKH ACPHCIKMF PTLDKLKMH LLRHSDHRE FLCADCGKQ FKRKDKLKE HCKRMHSEE RENDVPRPP KPVSQPVKK LNPKSEPTD FHRFIYKCH TCLVGFKRR GMLVNHLAK RHPDISPDS VPELNLPIL QTTRDYYCQ HCDKVYKSS SKRKAHILK NHPGAALPM SNRKQGNFP DNSGLPNPT FSQTVGSIT TRPQNCKWC HKQYASKAK LLQHQRKKH SEQLAQQNG GKEEQKYTP PEQAEGQQN DLQDFKVSE SDILNSALE EYNKRGGEQ FCHFVAIEG GDEFEQSTG DLGAPNSHL FRLLTTSNE LPGLVPPR
- >TC012726 RA
atggatacg attgtgcaa gggcccccc ggcgaaggg gcccaggac ttgaaccta gtcgacgcc gcttggagc acccaaaac tcgcaagag atcgtaaac aacaactct atcctttat attgctgtc gagtacatc aaagacgag tacaagatg gagtcggcg gtcaacttg ccgcccccg gagaacttc gccgatttc gagcagcac gtgagcccc ctcgacccc aacatgagc tccgtggcg cgctacagc cccgtgtac agcgagccc accactgag tacaacccc gtggtgatc caccacctg gtccagcca aattccagt cagagcgat attaataat ttagccccc gaacccatg cctgtctta tgcaataac gacatccgg gaagtcgtt gataaccat tttttgcaa atggtcaac tccgggaac cagctgcag gtggtgccc cccaacgag caggaggtg gcgctgctg ataactgac caggaaacg ggcatttcg tacagcgta agggggcag gagtttctc gttgaggac gagcagttg ctaaatgcg ctcagtccc gacccgctt ttggacccc catttgctg gcacttgac gaaaacgcg ctcaaaacc gagctgggg gatgaaata attaactca accgttaac acagccgtt aataactat atcacgagt ttgagtgcg gccgttgat aacgacgga cagtacaag atggagacc aggaggcag aaacagaac gaggtcgag tttgaagat cagttattg tccaaggtt tatagcatt gtggacaag ccggtgccg tcgagggcc agggccacg ctgccggag tcgtatctc actctgagc aaggtggac caagcactg ggcgttttt gccaagaaa acaatccct cgaagcaca cagttcgga cctttgact ggaattctg aaaccgagc accgaacgt ttgaaaaac ggtccgacg tctttgcaa ttttttatc gaagatgag aacaacgtc gtgtcacag attgatgtt tcggatgaa aatgcttcg aattggatg cgttttatc agacaagcg acgaattat aacgagcaa aatttattg ataacgcag gaaggcaac tctttgtat ttcacaacg acgaggagc attcttccg aagcaggaa ctgaaggtt ggctacagt gtgccctac gccacccgt cacaaatta tcaattctg gtccccgag gaagaaaaa tcgtggcct tgttacgaa tgttcggaa acttttgcc tcctctgat gaattacag aaacatttg aacatccac gacagtgac gaggaaaac aaatggaag aaaaagaat ttaaaaaat aagaaaaaa atcccaaaa agtgagacg tttcaatgt aataattgc aacgaactg ttcgtccag ccgggaaaa gtcgccctc aggcaacac ttgattgaa aaacacctt ttgagcggt ttggacttg atcgatcag tatttttct tcagtcatg aattacaag tgcgataaa tgcgaattg gttttcaat tcggaacct ttgttaaaa atccataac tatcttcat gattcggac agttcggac gaacaaacg aatcatgtt tgtcccaac tgtcagaaa aaattccca acgcaacgc caacttgtt acgcacgtc gctacgcac gcacttacc aaaaaaccg gaaacggaa acgttcaag tgtccagtg tgccacaaa atgttcgct atgcgcgag cgactaagg cgccacatg ctggtgcac ggctctgac gactcgaag cctctccag tgtaaaact tgcaataaa cgctttgtg aacaattcg gcgctcgcc ggccacatc aagacccac cttgtgggc aaaaaaatc ttcgaatgt cccatttgt aaagaaaat ttcgaccac gtcctaaaa ctgaaactc cacgtcccc aaacactgc gaaaataac acatactcg tgccctcat tgcagcaaa gttttcaag aagtacagt atcatacgc aaacatatc cgtgcgttt cactgcgac cagaaacac gcatgtccc cactgtatt aaaatgttc ccgactttg gataaatta aaaatgcat ttattgcgc cattcggac caccgggag tttttatgc gccgattgt ggcaagcag ttcaagcgc aaagacaag ctgaaagag cactgcaag cgcatgcat tcggaggag cgcgaaaac gacgttcca aggcccccg aaacccgtc tctcaaccg gtgaaaaaa ctcaatccg aagagcgaa ccgaccgat ttccaccga ttcatctac aagtgtcac acgtgtctt gttgggttt aaacgtagg gggatgttg gttaaccac ttggcgaag cgacacccg gatataagc ccggattcg gtcccggaa ttgaattta ccgattttg caaacgacg cgtgactat tattgccaa cactgcgat aaagtttat aagagtagt tcgaaacgc aaagctcac attttgaag aatcatccg ggggccgct ttgccgatg agtaaccgg aaacagggc aattttccg gataattcc gggcttccg aatccgacg ttttcgcaa actgtgggc agtattacg acgcggccg cagaactgc aagtggtgt cataagcag tatgcgagc aaagcgaag ttgttgcag catcagagg aagaagcac agcgagcag ttggctcag cagaatggg ggcaaggag gagcagaag tacacgccg ccggagcag gcggaggga caacagaat gacttgcag gactttaag gtgtctgag agcgatatt ttgaacagc gccctcgaa gaatataac aaacgcgga ggggagcag ttttgtcat tttgttgcg attgaaggt ggggacgag tttgagcag tcaactggg gatttgggg gcccccaat tcgcatttg tttcgctta ctgacaacg agcaacgaa cttccaggt ttagtgcca ccacgttga
- >TC012726 RA
caactctgg aaaactgtt tatttattg ggaaaaatt tcagctgat tgtgggttt gtaatggat acgattgtg caagggccc cccggcgaa ggggcccag gacttgaac ctagtcgac gccgcttgg agcacccaa aactcgcaa gagatcgta aacaacaac tctatcctt tatattgct gtcgagtac atcaaagac gagtacaag atggagtcg gcggtcaac ttgccgccc ccggagaac ttcgccgat ttcgagcag cacgtgagc cccctcgac cccaacatg agctccgtg gcgcgctac agccccgtg tacagcgag cccaccact gagtacaac cccgtggtg atccaccac ctggtccag ccaaattcc agtcagagc gatattaat aatttagcc cccgaaccc atgcctgtc ttatgcaat aacgacatc cgggaagtc gttgataac cattttttg caaatggtc aactccggg aaccagctg caggtggtg ccccccaac gagcaggag gtggcgctg ctgataact gaccaggaa acgggcatt tcgtacagc gtaaggggg caggagttt ctcgttgag gacgagcag ttgctaaat gcgctcagt cccgacccg cttttggac ccccatttg ctggcactt gacgaaaac gcgctcaaa accgagctg ggggatgaa ataattaac tcaaccgtt aacacagcc gttaataac tatatcacg agtttgagt gcggccgtt gataacgac ggacagtac aagatggag accaggagg cagaaacag aacgaggtc gagtttgaa gatcagtta ttgtccaag gtttatagc attgtggac aagccggtg ccgtcgagg gccagggcc acgctgccg gagtcgtat ctcactctg agcaaggtg gaccaagca ctgggcgtt tttgccaag aaaacaatc cctcgaagc acacagttc ggacctttg actggaatt ctgaaaccg agcaccgaa cgtttgaaa aacggtccg acgtctttg caatttttt atcgaagat gagaacaac gtcgtgtca cagattgat gtttcggat gaaaatgct tcgaattgg atgcgtttt atcagacaa gcgacgaat tataacgag caaaattta ttgataacg caggaaggc aactctttg tatttcaca acgacgagg agcattctt ccgaagcag gaactgaag gttggctac agtgtgccc tacgccacc cgtcacaaa ttatcaatt ctggtcccc gaggaagaa aaatcgtgg ccttgttac gaatgttcg gaaactttt gcctcctct gatgaatta cagaaacat ttgaacatc cacgacagt gacgaggaa aacaaatgg aagaaaaag aatttaaaa aataagaaa aaaatccca aaaagtgag acgtttcaa tgtaataat tgcaacgaa ctgttcgtc cagccggga aaagtcgcc ctcaggcaa cacttgatt gaaaaacac cttttgagc ggtttggac ttgatcgat cagtatttt tcttcagtc atgaattac aagtgcgat aaatgcgaa ttggttttc aattcggaa cctttgtta aaaatccat aactatctt catgattcg gacagttcg gacgaacaa acgaatcat gtttgtccc aactgtcag aaaaaattc ccaacgcaa cgccaactt gttacgcac gtcgctacg cacgcactt accaaaaaa ccggaaacg gaaacgttc aagtgtcca gtgtgccac aaaatgttc gctatgcgc gagcgacta aggcgccac atgctggtg cacggctct gacgactcg aagcctctc cagtgtaaa acttgcaat aaacgcttt gtgaacaat tcggcgctc gccggccac atcaagacc caccttgtg ggcaaaaaa atcttcgaa tgtcccatt tgtaaagaa aatttcgac cacgtccta aaactgaaa ctccacgtc cccaaacac tgcgaaaat aacacatac tcgtgccct cattgcagc aaagttttc aagaagtac agtatcata cgcaaacat atccgtgcg tttcactgc gaccagaaa cacgcatgt ccccactgt attaaaatg ttcccgact ttggataaa ttaaaaatg catttattg cgccattcg gaccaccgg gagttttta tgcgccgat tgtggcaag cagttcaag cgcaaagac aagctgaaa gagcactgc aagcgcatg cattcggag gagcgcgaa aacgacgtt ccaaggccc ccgaaaccc gtctctcaa ccggtgaaa aaactcaat ccgaagagc gaaccgacc gatttccac cgattcatc tacaagtgt cacacgtgt cttgttggg tttaaacgt agggggatg ttggttaac cacttggcg aagcgacac ccggatata agcccggat tcggtcccg gaattgaat ttaccgatt ttgcaaacg acgcgtgac tattattgc caacactgc gataaagtt tataagagt agttcgaaa cgcaaagct cacattttg aagaatcat ccgggggcc gctttgccg atgagtaac cggaaacag ggcaatttt ccggataat tccgggctt ccgaatccg acgttttcg caaactgtg ggcagtatt acgacgcgg ccgcagaac tgcaagtgg tgtcataag cagtatgcg agcaaagcg aagttgttg cagcatcag aggaagaag cacagcgag cagttggct cagcagaat gggggcaag gaggagcag aagtacacg ccgccggag caggcggag ggacaacag aatgacttg caggacttt aaggtgtct gagagcgat attttgaac agcgccctc gaagaatat aacaaacgc ggaggggag cagttttgt cattttgtt gcgattgaa ggtggggac gagtttgag cagtcaact ggggatttg ggggccccc aattcgcat ttgtttcgc ttactgaca acgagcaac gaacttcca ggtttagtg ccaccacgt tgaagaaaa acctcgagg atttcgagt atttccggt gctcctttg tgagatttc cactcaact gagtgattt tgagcgagt ttgtgccgt aggttgaaa cctacgtat ttaataaaa tgcaaaaag taacaaaat agaacgaga ggcgaagag aattttggg aaattgaga ttattttag caataattg tcttaattg taacaaatt tataaacga acaagcgct tattgtttg gcttggccg cggccgctt gtgtttagc ttagggacg tttgtttgg gtcaaaaat gtatcctag attctaaaa gaaatgaaa ccttttccc cgtgatagt actaacact ttgtatttt aacacgtaa ataattagg gaaatgtaa ccctgtgat tgttgaaaa caaag
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
