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Gene information
General information
TC012726 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 13712951..13719345
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC012726
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05304

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: The labium seems to be the most affected head part.
    labium not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012726 RA
      MDTIVQGPP GEGAQDLNL VDAAWSTQN SQEIVNNNS 
ILYIAVEYI KDEYKMESA VNLPPPENF ADFEQHVSP 
LDPNMSSVA RYSPVYSEP TTEYNPVVI HHLVQPNSS 
QSDINNLAP EPMPVLCNN DIREVVDNH FLQMVNSGN 
QLQVVPPNE QEVALLITD QETGISYSV RGQEFLVED 
EQLLNALSP DPLLDPHLL ALDENALKT ELGDEIINS 
TVNTAVNNY ITSLSAAVD NDGQYKMET RRQKQNEVE 
FEDQLLSKV YSIVDKPVP SRARATLPE SYLTLSKVD 
QALGVFAKK TIPRSTQFG PLTGILKPS TERLKNGPT 
SLQFFIEDE NNVVSQIDV SDENASNWM RFIRQATNY 
NEQNLLITQ EGNSLYFTT TRSILPKQE LKVGYSVPY 
ATRHKLSIL VPEEEKSWP CYECSETFA SSDELQKHL 
NIHDSDEEN KWKKKNLKN KKKIPKSET FQCNNCNEL 
FVQPGKVAL RQHLIEKHL LSGLDLIDQ YFSSVMNYK 
CDKCELVFN SEPLLKIHN YLHDSDSSD EQTNHVCPN 
CQKKFPTQR QLVTHVATH ALTKKPETE TFKCPVCHK 
MFAMRERLR RHMLVHGSD DSKPLQCKT CNKRFVNNS 
ALAGHIKTH LVGKKIFEC PICKENFDH VLKLKLHVP 
KHCENNTYS CPHCSKVFK KYSIIRKHI RAFHCDQKH 
ACPHCIKMF PTLDKLKMH LLRHSDHRE FLCADCGKQ 
FKRKDKLKE HCKRMHSEE RENDVPRPP KPVSQPVKK 
LNPKSEPTD FHRFIYKCH TCLVGFKRR GMLVNHLAK 
RHPDISPDS VPELNLPIL QTTRDYYCQ HCDKVYKSS 
SKRKAHILK NHPGAALPM SNRKQGNFP DNSGLPNPT 
FSQTVGSIT TRPQNCKWC HKQYASKAK LLQHQRKKH 
SEQLAQQNG GKEEQKYTP PEQAEGQQN DLQDFKVSE 
SDILNSALE EYNKRGGEQ FCHFVAIEG GDEFEQSTG 
DLGAPNSHL FRLLTTSNE LPGLVPPR
    CDS sequence
    • >TC012726 RA
      atggatacg attgtgcaa gggcccccc ggcgaaggg 
gcccaggac ttgaaccta gtcgacgcc gcttggagc 
acccaaaac tcgcaagag atcgtaaac aacaactct 
atcctttat attgctgtc gagtacatc aaagacgag 
tacaagatg gagtcggcg gtcaacttg ccgcccccg 
gagaacttc gccgatttc gagcagcac gtgagcccc 
ctcgacccc aacatgagc tccgtggcg cgctacagc 
cccgtgtac agcgagccc accactgag tacaacccc 
gtggtgatc caccacctg gtccagcca aattccagt 
cagagcgat attaataat ttagccccc gaacccatg 
cctgtctta tgcaataac gacatccgg gaagtcgtt 
gataaccat tttttgcaa atggtcaac tccgggaac 
cagctgcag gtggtgccc cccaacgag caggaggtg 
gcgctgctg ataactgac caggaaacg ggcatttcg 
tacagcgta agggggcag gagtttctc gttgaggac 
gagcagttg ctaaatgcg ctcagtccc gacccgctt 
ttggacccc catttgctg gcacttgac gaaaacgcg 
ctcaaaacc gagctgggg gatgaaata attaactca 
accgttaac acagccgtt aataactat atcacgagt 
ttgagtgcg gccgttgat aacgacgga cagtacaag 
atggagacc aggaggcag aaacagaac gaggtcgag 
tttgaagat cagttattg tccaaggtt tatagcatt 
gtggacaag ccggtgccg tcgagggcc agggccacg 
ctgccggag tcgtatctc actctgagc aaggtggac 
caagcactg ggcgttttt gccaagaaa acaatccct 
cgaagcaca cagttcgga cctttgact ggaattctg 
aaaccgagc accgaacgt ttgaaaaac ggtccgacg 
tctttgcaa ttttttatc gaagatgag aacaacgtc 
gtgtcacag attgatgtt tcggatgaa aatgcttcg 
aattggatg cgttttatc agacaagcg acgaattat 
aacgagcaa aatttattg ataacgcag gaaggcaac 
tctttgtat ttcacaacg acgaggagc attcttccg 
aagcaggaa ctgaaggtt ggctacagt gtgccctac 
gccacccgt cacaaatta tcaattctg gtccccgag 
gaagaaaaa tcgtggcct tgttacgaa tgttcggaa 
acttttgcc tcctctgat gaattacag aaacatttg 
aacatccac gacagtgac gaggaaaac aaatggaag 
aaaaagaat ttaaaaaat aagaaaaaa atcccaaaa 
agtgagacg tttcaatgt aataattgc aacgaactg 
ttcgtccag ccgggaaaa gtcgccctc aggcaacac 
ttgattgaa aaacacctt ttgagcggt ttggacttg 
atcgatcag tatttttct tcagtcatg aattacaag 
tgcgataaa tgcgaattg gttttcaat tcggaacct 
ttgttaaaa atccataac tatcttcat gattcggac 
agttcggac gaacaaacg aatcatgtt tgtcccaac 
tgtcagaaa aaattccca acgcaacgc caacttgtt 
acgcacgtc gctacgcac gcacttacc aaaaaaccg 
gaaacggaa acgttcaag tgtccagtg tgccacaaa 
atgttcgct atgcgcgag cgactaagg cgccacatg 
ctggtgcac ggctctgac gactcgaag cctctccag 
tgtaaaact tgcaataaa cgctttgtg aacaattcg 
gcgctcgcc ggccacatc aagacccac cttgtgggc 
aaaaaaatc ttcgaatgt cccatttgt aaagaaaat 
ttcgaccac gtcctaaaa ctgaaactc cacgtcccc 
aaacactgc gaaaataac acatactcg tgccctcat 
tgcagcaaa gttttcaag aagtacagt atcatacgc 
aaacatatc cgtgcgttt cactgcgac cagaaacac 
gcatgtccc cactgtatt aaaatgttc ccgactttg 
gataaatta aaaatgcat ttattgcgc cattcggac 
caccgggag tttttatgc gccgattgt ggcaagcag 
ttcaagcgc aaagacaag ctgaaagag cactgcaag 
cgcatgcat tcggaggag cgcgaaaac gacgttcca 
aggcccccg aaacccgtc tctcaaccg gtgaaaaaa 
ctcaatccg aagagcgaa ccgaccgat ttccaccga 
ttcatctac aagtgtcac acgtgtctt gttgggttt 
aaacgtagg gggatgttg gttaaccac ttggcgaag 
cgacacccg gatataagc ccggattcg gtcccggaa 
ttgaattta ccgattttg caaacgacg cgtgactat 
tattgccaa cactgcgat aaagtttat aagagtagt 
tcgaaacgc aaagctcac attttgaag aatcatccg 
ggggccgct ttgccgatg agtaaccgg aaacagggc 
aattttccg gataattcc gggcttccg aatccgacg 
ttttcgcaa actgtgggc agtattacg acgcggccg 
cagaactgc aagtggtgt cataagcag tatgcgagc 
aaagcgaag ttgttgcag catcagagg aagaagcac 
agcgagcag ttggctcag cagaatggg ggcaaggag 
gagcagaag tacacgccg ccggagcag gcggaggga 
caacagaat gacttgcag gactttaag gtgtctgag 
agcgatatt ttgaacagc gccctcgaa gaatataac 
aaacgcgga ggggagcag ttttgtcat tttgttgcg 
attgaaggt ggggacgag tttgagcag tcaactggg 
gatttgggg gcccccaat tcgcatttg tttcgctta 
ctgacaacg agcaacgaa cttccaggt ttagtgcca 
ccacgttga
    mRNA sequence
    • >TC012726 RA
      caactctgg aaaactgtt tatttattg ggaaaaatt 
tcagctgat tgtgggttt gtaatggat acgattgtg 
caagggccc cccggcgaa ggggcccag gacttgaac 
ctagtcgac gccgcttgg agcacccaa aactcgcaa 
gagatcgta aacaacaac tctatcctt tatattgct 
gtcgagtac atcaaagac gagtacaag atggagtcg 
gcggtcaac ttgccgccc ccggagaac ttcgccgat 
ttcgagcag cacgtgagc cccctcgac cccaacatg 
agctccgtg gcgcgctac agccccgtg tacagcgag 
cccaccact gagtacaac cccgtggtg atccaccac 
ctggtccag ccaaattcc agtcagagc gatattaat 
aatttagcc cccgaaccc atgcctgtc ttatgcaat 
aacgacatc cgggaagtc gttgataac cattttttg 
caaatggtc aactccggg aaccagctg caggtggtg 
ccccccaac gagcaggag gtggcgctg ctgataact 
gaccaggaa acgggcatt tcgtacagc gtaaggggg 
caggagttt ctcgttgag gacgagcag ttgctaaat 
gcgctcagt cccgacccg cttttggac ccccatttg 
ctggcactt gacgaaaac gcgctcaaa accgagctg 
ggggatgaa ataattaac tcaaccgtt aacacagcc 
gttaataac tatatcacg agtttgagt gcggccgtt 
gataacgac ggacagtac aagatggag accaggagg 
cagaaacag aacgaggtc gagtttgaa gatcagtta 
ttgtccaag gtttatagc attgtggac aagccggtg 
ccgtcgagg gccagggcc acgctgccg gagtcgtat 
ctcactctg agcaaggtg gaccaagca ctgggcgtt 
tttgccaag aaaacaatc cctcgaagc acacagttc 
ggacctttg actggaatt ctgaaaccg agcaccgaa 
cgtttgaaa aacggtccg acgtctttg caatttttt 
atcgaagat gagaacaac gtcgtgtca cagattgat 
gtttcggat gaaaatgct tcgaattgg atgcgtttt 
atcagacaa gcgacgaat tataacgag caaaattta 
ttgataacg caggaaggc aactctttg tatttcaca 
acgacgagg agcattctt ccgaagcag gaactgaag 
gttggctac agtgtgccc tacgccacc cgtcacaaa 
ttatcaatt ctggtcccc gaggaagaa aaatcgtgg 
ccttgttac gaatgttcg gaaactttt gcctcctct 
gatgaatta cagaaacat ttgaacatc cacgacagt 
gacgaggaa aacaaatgg aagaaaaag aatttaaaa 
aataagaaa aaaatccca aaaagtgag acgtttcaa 
tgtaataat tgcaacgaa ctgttcgtc cagccggga 
aaagtcgcc ctcaggcaa cacttgatt gaaaaacac 
cttttgagc ggtttggac ttgatcgat cagtatttt 
tcttcagtc atgaattac aagtgcgat aaatgcgaa 
ttggttttc aattcggaa cctttgtta aaaatccat 
aactatctt catgattcg gacagttcg gacgaacaa 
acgaatcat gtttgtccc aactgtcag aaaaaattc 
ccaacgcaa cgccaactt gttacgcac gtcgctacg 
cacgcactt accaaaaaa ccggaaacg gaaacgttc 
aagtgtcca gtgtgccac aaaatgttc gctatgcgc 
gagcgacta aggcgccac atgctggtg cacggctct 
gacgactcg aagcctctc cagtgtaaa acttgcaat 
aaacgcttt gtgaacaat tcggcgctc gccggccac 
atcaagacc caccttgtg ggcaaaaaa atcttcgaa 
tgtcccatt tgtaaagaa aatttcgac cacgtccta 
aaactgaaa ctccacgtc cccaaacac tgcgaaaat 
aacacatac tcgtgccct cattgcagc aaagttttc 
aagaagtac agtatcata cgcaaacat atccgtgcg 
tttcactgc gaccagaaa cacgcatgt ccccactgt 
attaaaatg ttcccgact ttggataaa ttaaaaatg 
catttattg cgccattcg gaccaccgg gagttttta 
tgcgccgat tgtggcaag cagttcaag cgcaaagac 
aagctgaaa gagcactgc aagcgcatg cattcggag 
gagcgcgaa aacgacgtt ccaaggccc ccgaaaccc 
gtctctcaa ccggtgaaa aaactcaat ccgaagagc 
gaaccgacc gatttccac cgattcatc tacaagtgt 
cacacgtgt cttgttggg tttaaacgt agggggatg 
ttggttaac cacttggcg aagcgacac ccggatata 
agcccggat tcggtcccg gaattgaat ttaccgatt 
ttgcaaacg acgcgtgac tattattgc caacactgc 
gataaagtt tataagagt agttcgaaa cgcaaagct 
cacattttg aagaatcat ccgggggcc gctttgccg 
atgagtaac cggaaacag ggcaatttt ccggataat 
tccgggctt ccgaatccg acgttttcg caaactgtg 
ggcagtatt acgacgcgg ccgcagaac tgcaagtgg 
tgtcataag cagtatgcg agcaaagcg aagttgttg 
cagcatcag aggaagaag cacagcgag cagttggct 
cagcagaat gggggcaag gaggagcag aagtacacg 
ccgccggag caggcggag ggacaacag aatgacttg 
caggacttt aaggtgtct gagagcgat attttgaac 
agcgccctc gaagaatat aacaaacgc ggaggggag 
cagttttgt cattttgtt gcgattgaa ggtggggac 
gagtttgag cagtcaact ggggatttg ggggccccc 
aattcgcat ttgtttcgc ttactgaca acgagcaac 
gaacttcca ggtttagtg ccaccacgt tgaagaaaa 
acctcgagg atttcgagt atttccggt gctcctttg 
tgagatttc cactcaact gagtgattt tgagcgagt 
ttgtgccgt aggttgaaa cctacgtat ttaataaaa 
tgcaaaaag taacaaaat agaacgaga ggcgaagag 
aattttggg aaattgaga ttattttag caataattg 
tcttaattg taacaaatt tataaacga acaagcgct 
tattgtttg gcttggccg cggccgctt gtgtttagc 
ttagggacg tttgtttgg gtcaaaaat gtatcctag 
attctaaaa gaaatgaaa ccttttccc cgtgatagt 
actaacact ttgtatttt aacacgtaa ataattagg 
gaaatgtaa ccctgtgat tgttgaaaa caaag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY