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Gene information
General information
TC012726 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 13712951..13719345
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012726
    iBeetle screen iB_05304

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: The labium seems to be the most affected head part.
    labium not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012726 RA
      MDTIVQGPP GEGAQDLNL VDAAWSTQN SQEIVNNNS 
      ILYIAVEYI KDEYKMESA VNLPPPENF ADFEQHVSP 
      LDPNMSSVA RYSPVYSEP TTEYNPVVI HHLVQPNSS 
      QSDINNLAP EPMPVLCNN DIREVVDNH FLQMVNSGN 
      QLQVVPPNE QEVALLITD QETGISYSV RGQEFLVED 
      EQLLNALSP DPLLDPHLL ALDENALKT ELGDEIINS 
      TVNTAVNNY ITSLSAAVD NDGQYKMET RRQKQNEVE 
      FEDQLLSKV YSIVDKPVP SRARATLPE SYLTLSKVD 
      QALGVFAKK TIPRSTQFG PLTGILKPS TERLKNGPT 
      SLQFFIEDE NNVVSQIDV SDENASNWM RFIRQATNY 
      NEQNLLITQ EGNSLYFTT TRSILPKQE LKVGYSVPY 
      ATRHKLSIL VPEEEKSWP CYECSETFA SSDELQKHL 
      NIHDSDEEN KWKKKNLKN KKKIPKSET FQCNNCNEL 
      FVQPGKVAL RQHLIEKHL LSGLDLIDQ YFSSVMNYK 
      CDKCELVFN SEPLLKIHN YLHDSDSSD EQTNHVCPN 
      CQKKFPTQR QLVTHVATH ALTKKPETE TFKCPVCHK 
      MFAMRERLR RHMLVHGSD DSKPLQCKT CNKRFVNNS 
      ALAGHIKTH LVGKKIFEC PICKENFDH VLKLKLHVP 
      KHCENNTYS CPHCSKVFK KYSIIRKHI RAFHCDQKH 
      ACPHCIKMF PTLDKLKMH LLRHSDHRE FLCADCGKQ 
      FKRKDKLKE HCKRMHSEE RENDVPRPP KPVSQPVKK 
      LNPKSEPTD FHRFIYKCH TCLVGFKRR GMLVNHLAK 
      RHPDISPDS VPELNLPIL QTTRDYYCQ HCDKVYKSS 
      SKRKAHILK NHPGAALPM SNRKQGNFP DNSGLPNPT 
      FSQTVGSIT TRPQNCKWC HKQYASKAK LLQHQRKKH 
      SEQLAQQNG GKEEQKYTP PEQAEGQQN DLQDFKVSE 
      SDILNSALE EYNKRGGEQ FCHFVAIEG GDEFEQSTG 
      DLGAPNSHL FRLLTTSNE LPGLVPPR
    CDS sequence
    • >TC012726 RA
      atggatacg attgtgcaa gggcccccc ggcgaaggg 
      gcccaggac ttgaaccta gtcgacgcc gcttggagc 
      acccaaaac tcgcaagag atcgtaaac aacaactct 
      atcctttat attgctgtc gagtacatc aaagacgag 
      tacaagatg gagtcggcg gtcaacttg ccgcccccg 
      gagaacttc gccgatttc gagcagcac gtgagcccc 
      ctcgacccc aacatgagc tccgtggcg cgctacagc 
      cccgtgtac agcgagccc accactgag tacaacccc 
      gtggtgatc caccacctg gtccagcca aattccagt 
      cagagcgat attaataat ttagccccc gaacccatg 
      cctgtctta tgcaataac gacatccgg gaagtcgtt 
      gataaccat tttttgcaa atggtcaac tccgggaac 
      cagctgcag gtggtgccc cccaacgag caggaggtg 
      gcgctgctg ataactgac caggaaacg ggcatttcg 
      tacagcgta agggggcag gagtttctc gttgaggac 
      gagcagttg ctaaatgcg ctcagtccc gacccgctt 
      ttggacccc catttgctg gcacttgac gaaaacgcg 
      ctcaaaacc gagctgggg gatgaaata attaactca 
      accgttaac acagccgtt aataactat atcacgagt 
      ttgagtgcg gccgttgat aacgacgga cagtacaag 
      atggagacc aggaggcag aaacagaac gaggtcgag 
      tttgaagat cagttattg tccaaggtt tatagcatt 
      gtggacaag ccggtgccg tcgagggcc agggccacg 
      ctgccggag tcgtatctc actctgagc aaggtggac 
      caagcactg ggcgttttt gccaagaaa acaatccct 
      cgaagcaca cagttcgga cctttgact ggaattctg 
      aaaccgagc accgaacgt ttgaaaaac ggtccgacg 
      tctttgcaa ttttttatc gaagatgag aacaacgtc 
      gtgtcacag attgatgtt tcggatgaa aatgcttcg 
      aattggatg cgttttatc agacaagcg acgaattat 
      aacgagcaa aatttattg ataacgcag gaaggcaac 
      tctttgtat ttcacaacg acgaggagc attcttccg 
      aagcaggaa ctgaaggtt ggctacagt gtgccctac 
      gccacccgt cacaaatta tcaattctg gtccccgag 
      gaagaaaaa tcgtggcct tgttacgaa tgttcggaa 
      acttttgcc tcctctgat gaattacag aaacatttg 
      aacatccac gacagtgac gaggaaaac aaatggaag 
      aaaaagaat ttaaaaaat aagaaaaaa atcccaaaa 
      agtgagacg tttcaatgt aataattgc aacgaactg 
      ttcgtccag ccgggaaaa gtcgccctc aggcaacac 
      ttgattgaa aaacacctt ttgagcggt ttggacttg 
      atcgatcag tatttttct tcagtcatg aattacaag 
      tgcgataaa tgcgaattg gttttcaat tcggaacct 
      ttgttaaaa atccataac tatcttcat gattcggac 
      agttcggac gaacaaacg aatcatgtt tgtcccaac 
      tgtcagaaa aaattccca acgcaacgc caacttgtt 
      acgcacgtc gctacgcac gcacttacc aaaaaaccg 
      gaaacggaa acgttcaag tgtccagtg tgccacaaa 
      atgttcgct atgcgcgag cgactaagg cgccacatg 
      ctggtgcac ggctctgac gactcgaag cctctccag 
      tgtaaaact tgcaataaa cgctttgtg aacaattcg 
      gcgctcgcc ggccacatc aagacccac cttgtgggc 
      aaaaaaatc ttcgaatgt cccatttgt aaagaaaat 
      ttcgaccac gtcctaaaa ctgaaactc cacgtcccc 
      aaacactgc gaaaataac acatactcg tgccctcat 
      tgcagcaaa gttttcaag aagtacagt atcatacgc 
      aaacatatc cgtgcgttt cactgcgac cagaaacac 
      gcatgtccc cactgtatt aaaatgttc ccgactttg 
      gataaatta aaaatgcat ttattgcgc cattcggac 
      caccgggag tttttatgc gccgattgt ggcaagcag 
      ttcaagcgc aaagacaag ctgaaagag cactgcaag 
      cgcatgcat tcggaggag cgcgaaaac gacgttcca 
      aggcccccg aaacccgtc tctcaaccg gtgaaaaaa 
      ctcaatccg aagagcgaa ccgaccgat ttccaccga 
      ttcatctac aagtgtcac acgtgtctt gttgggttt 
      aaacgtagg gggatgttg gttaaccac ttggcgaag 
      cgacacccg gatataagc ccggattcg gtcccggaa 
      ttgaattta ccgattttg caaacgacg cgtgactat 
      tattgccaa cactgcgat aaagtttat aagagtagt 
      tcgaaacgc aaagctcac attttgaag aatcatccg 
      ggggccgct ttgccgatg agtaaccgg aaacagggc 
      aattttccg gataattcc gggcttccg aatccgacg 
      ttttcgcaa actgtgggc agtattacg acgcggccg 
      cagaactgc aagtggtgt cataagcag tatgcgagc 
      aaagcgaag ttgttgcag catcagagg aagaagcac 
      agcgagcag ttggctcag cagaatggg ggcaaggag 
      gagcagaag tacacgccg ccggagcag gcggaggga 
      caacagaat gacttgcag gactttaag gtgtctgag 
      agcgatatt ttgaacagc gccctcgaa gaatataac 
      aaacgcgga ggggagcag ttttgtcat tttgttgcg 
      attgaaggt ggggacgag tttgagcag tcaactggg 
      gatttgggg gcccccaat tcgcatttg tttcgctta 
      ctgacaacg agcaacgaa cttccaggt ttagtgcca 
      ccacgttga
    mRNA sequence
    • >TC012726 RA
      caactctgg aaaactgtt tatttattg ggaaaaatt 
      tcagctgat tgtgggttt gtaatggat acgattgtg 
      caagggccc cccggcgaa ggggcccag gacttgaac 
      ctagtcgac gccgcttgg agcacccaa aactcgcaa 
      gagatcgta aacaacaac tctatcctt tatattgct 
      gtcgagtac atcaaagac gagtacaag atggagtcg 
      gcggtcaac ttgccgccc ccggagaac ttcgccgat 
      ttcgagcag cacgtgagc cccctcgac cccaacatg 
      agctccgtg gcgcgctac agccccgtg tacagcgag 
      cccaccact gagtacaac cccgtggtg atccaccac 
      ctggtccag ccaaattcc agtcagagc gatattaat 
      aatttagcc cccgaaccc atgcctgtc ttatgcaat 
      aacgacatc cgggaagtc gttgataac cattttttg 
      caaatggtc aactccggg aaccagctg caggtggtg 
      ccccccaac gagcaggag gtggcgctg ctgataact 
      gaccaggaa acgggcatt tcgtacagc gtaaggggg 
      caggagttt ctcgttgag gacgagcag ttgctaaat 
      gcgctcagt cccgacccg cttttggac ccccatttg 
      ctggcactt gacgaaaac gcgctcaaa accgagctg 
      ggggatgaa ataattaac tcaaccgtt aacacagcc 
      gttaataac tatatcacg agtttgagt gcggccgtt 
      gataacgac ggacagtac aagatggag accaggagg 
      cagaaacag aacgaggtc gagtttgaa gatcagtta 
      ttgtccaag gtttatagc attgtggac aagccggtg 
      ccgtcgagg gccagggcc acgctgccg gagtcgtat 
      ctcactctg agcaaggtg gaccaagca ctgggcgtt 
      tttgccaag aaaacaatc cctcgaagc acacagttc 
      ggacctttg actggaatt ctgaaaccg agcaccgaa 
      cgtttgaaa aacggtccg acgtctttg caatttttt 
      atcgaagat gagaacaac gtcgtgtca cagattgat 
      gtttcggat gaaaatgct tcgaattgg atgcgtttt 
      atcagacaa gcgacgaat tataacgag caaaattta 
      ttgataacg caggaaggc aactctttg tatttcaca 
      acgacgagg agcattctt ccgaagcag gaactgaag 
      gttggctac agtgtgccc tacgccacc cgtcacaaa 
      ttatcaatt ctggtcccc gaggaagaa aaatcgtgg 
      ccttgttac gaatgttcg gaaactttt gcctcctct 
      gatgaatta cagaaacat ttgaacatc cacgacagt 
      gacgaggaa aacaaatgg aagaaaaag aatttaaaa 
      aataagaaa aaaatccca aaaagtgag acgtttcaa 
      tgtaataat tgcaacgaa ctgttcgtc cagccggga 
      aaagtcgcc ctcaggcaa cacttgatt gaaaaacac 
      cttttgagc ggtttggac ttgatcgat cagtatttt 
      tcttcagtc atgaattac aagtgcgat aaatgcgaa 
      ttggttttc aattcggaa cctttgtta aaaatccat 
      aactatctt catgattcg gacagttcg gacgaacaa 
      acgaatcat gtttgtccc aactgtcag aaaaaattc 
      ccaacgcaa cgccaactt gttacgcac gtcgctacg 
      cacgcactt accaaaaaa ccggaaacg gaaacgttc 
      aagtgtcca gtgtgccac aaaatgttc gctatgcgc 
      gagcgacta aggcgccac atgctggtg cacggctct 
      gacgactcg aagcctctc cagtgtaaa acttgcaat 
      aaacgcttt gtgaacaat tcggcgctc gccggccac 
      atcaagacc caccttgtg ggcaaaaaa atcttcgaa 
      tgtcccatt tgtaaagaa aatttcgac cacgtccta 
      aaactgaaa ctccacgtc cccaaacac tgcgaaaat 
      aacacatac tcgtgccct cattgcagc aaagttttc 
      aagaagtac agtatcata cgcaaacat atccgtgcg 
      tttcactgc gaccagaaa cacgcatgt ccccactgt 
      attaaaatg ttcccgact ttggataaa ttaaaaatg 
      catttattg cgccattcg gaccaccgg gagttttta 
      tgcgccgat tgtggcaag cagttcaag cgcaaagac 
      aagctgaaa gagcactgc aagcgcatg cattcggag 
      gagcgcgaa aacgacgtt ccaaggccc ccgaaaccc 
      gtctctcaa ccggtgaaa aaactcaat ccgaagagc 
      gaaccgacc gatttccac cgattcatc tacaagtgt 
      cacacgtgt cttgttggg tttaaacgt agggggatg 
      ttggttaac cacttggcg aagcgacac ccggatata 
      agcccggat tcggtcccg gaattgaat ttaccgatt 
      ttgcaaacg acgcgtgac tattattgc caacactgc 
      gataaagtt tataagagt agttcgaaa cgcaaagct 
      cacattttg aagaatcat ccgggggcc gctttgccg 
      atgagtaac cggaaacag ggcaatttt ccggataat 
      tccgggctt ccgaatccg acgttttcg caaactgtg 
      ggcagtatt acgacgcgg ccgcagaac tgcaagtgg 
      tgtcataag cagtatgcg agcaaagcg aagttgttg 
      cagcatcag aggaagaag cacagcgag cagttggct 
      cagcagaat gggggcaag gaggagcag aagtacacg 
      ccgccggag caggcggag ggacaacag aatgacttg 
      caggacttt aaggtgtct gagagcgat attttgaac 
      agcgccctc gaagaatat aacaaacgc ggaggggag 
      cagttttgt cattttgtt gcgattgaa ggtggggac 
      gagtttgag cagtcaact ggggatttg ggggccccc 
      aattcgcat ttgtttcgc ttactgaca acgagcaac 
      gaacttcca ggtttagtg ccaccacgt tgaagaaaa 
      acctcgagg atttcgagt atttccggt gctcctttg 
      tgagatttc cactcaact gagtgattt tgagcgagt 
      ttgtgccgt aggttgaaa cctacgtat ttaataaaa 
      tgcaaaaag taacaaaat agaacgaga ggcgaagag 
      aattttggg aaattgaga ttattttag caataattg 
      tcttaattg taacaaatt tataaacga acaagcgct 
      tattgtttg gcttggccg cggccgctt gtgtttagc 
      ttagggacg tttgtttgg gtcaaaaat gtatcctag 
      attctaaaa gaaatgaaa ccttttccc cgtgatagt 
      actaacact ttgtatttt aacacgtaa ataattagg 
      gaaatgtaa ccctgtgat tgttgaaaa caaag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY