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Gene information
General information
TC012723 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 13695631..13702191
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012723
    iBeetle screen iB_05303

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 8 (9 dpi)
    Days post injection


    Pupal morphology
    beetle stock: bad (increased lethality),
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012723 RA
      MGDQTFHTP SFGDEDFDI PAINPQQQQ QQQQQQQQQ 
      QHTDQQQHY QQQPMQGMQ QMNQQMNQQ QDAMGMHDP 
      TGGYQQPLY LAGPDHGMV NPSYHSPQP NYSMSPSQQ 
      QQHNNQLLM IQQQQQQQH QQMMMNHGQ PPPGAMNQY 
      GTSPQHPQG RGNSPPAPG QEPTTSEDS DDSAPHTAM 
      MTAMKRPSP EPADQTLTK TQKKPKVQK KKKKRDPNE 
      PQKPVSAYA LFFRDTQAA IKGQNPNAS FGEVSKIVA 
      SMWDALDSE HKNVYKKKT EAAKKEYLK ALAAYRASL 
      VSKGAGEGD GMYGNYGSY NAGAPQYGG YTPQGAIPS 
      PPMSSAATP PTQQPPIGK KPAMMSQQQ QQQQHQNMM 
      QSPMGQVHM QHMQQQHNS YMQQVPQQQ HMPVSPQQP 
      HMQQHVSPP PMVSSSPPV SAAAPPPAT AAQQAQGQM 
      RPPNSCIRH GCPNPAINN AEWEDEYCS NECVVSHCR 
      DVFTNWVAN NKNQSQNFS TVK
    CDS sequence
    • >TC012723 RA
      atgggcgac cagacgttt catacgccg agtttcggc 
      gacgaagac ttcgacatt ccggcgata aatccgcag 
      cagcagcag cagcagcag caacagcag cagcaacag 
      cagcacacc gaccagcag cagcactac caacagcag 
      ccaatgcaa ggcatgcaa caaatgaac caacaaatg 
      aaccaacag caggacgca atgggcatg cacgaccca 
      acgggtggc taccaacag ccgttgtat ctcgcaggt 
      cccgatcac gggatggtc aatccgtcc tatcacagt 
      ccacagccg aattactcg atgagtcct agccaacaa 
      caacagcac aacaatcaa ctgttgatg atacagcaa 
      caacaacag cagcaacat cagcagatg atgatgaat 
      catggacaa cctccaccg ggagccatg aatcagtac 
      gggacgtcg ccacaacat ccccaagga aggggcaac 
      agcccgccg gcccctgga caggaaccc accaccagc 
      gaggacagc gacgacagc gccccgcac acggctatg 
      atgactgcg atgaagcgg ccgtcgccc gaacccgcg 
      gaccaaacg ttaacgaaa acgcagaaa aagcccaaa 
      gtgcagaag aagaaaaag aagcgggac cccaacgag 
      ccccaaaaa cccgtctcg gcttacgcc ttgttcttc 
      cgtgatacg caagcggcc atcaaggga caaaacccg 
      aacgcgagt ttcggggaa gtctcgaaa atcgttgct 
      tccatgtgg gacgctttg gactcggaa cataaaaac 
      gtgtataaa aagaagacc gaagcggcc aagaaagaa 
      tacctgaaa gcgctcgcc gcctaccgg gccagttta 
      gtgtcgaaa ggcgccggc gagggcgac ggcatgtac 
      ggcaactac ggcagttac aacgccggc gccccccaa 
      tacgggggc tacaccccg caaggcgcc atcccgtcg 
      ccccccatg tcctcagcg gcgacgccg cccacccag 
      cagcccccc atcggcaag aagccggcc atgatgtcg 
      caacagcaa cagcaacaa cagcaccaa aacatgatg 
      cagtcgcca atgggccaa gtgcacatg cagcacatg 
      cagcaacag cacaacagc tacatgcaa caggttcct 
      caacagcag cacatgccc gtgtcgccg cagcagccg 
      cacatgcag cagcacgtc agtccgccc ccgatggtc 
      tcgagttcg ccgcccgtc tcggcggcg gcgccgccc 
      ccggccacc gccgcccag caggcccaa ggacagatg 
      agacccccc aactcgtgt atacggcac ggctgcccc 
      aacccggcc atcaacaac gccgagtgg gaggacgag 
      tactgcagt aatgagtgc gtcgtgagc cattgccgg 
      gatgtattc acaaactgg gtagcgaac aacaagaac 
      caatcacaa aatttttcg acagttaaa taa
    mRNA sequence
    • >TC012723 RA
      ccgcacaac tcagcaatt tttttttgc tttcagaga 
      tccgaaaac gtggacttg tctctgagc ataccccag 
      tccaacttc caatccaac ggctacgac atgggcgac 
      cagacgttt catacgccg agtttcggc gacgaagac 
      ttcgacatt ccggcgata aatccgcag cagcagcag 
      cagcagcag caacagcag cagcaacag cagcacacc 
      gaccagcag cagcactac caacagcag ccaatgcaa 
      ggcatgcaa caaatgaac caacaaatg aaccaacag 
      caggacgca atgggcatg cacgaccca acgggtggc 
      taccaacag ccgttgtat ctcgcaggt cccgatcac 
      gggatggtc aatccgtcc tatcacagt ccacagccg 
      aattactcg atgagtcct agccaacaa caacagcac 
      aacaatcaa ctgttgatg atacagcaa caacaacag 
      cagcaacat cagcagatg atgatgaat catggacaa 
      cctccaccg ggagccatg aatcagtac gggacgtcg 
      ccacaacat ccccaagga aggggcaac agcccgccg 
      gcccctgga caggaaccc accaccagc gaggacagc 
      gacgacagc gccccgcac acggctatg atgactgcg 
      atgaagcgg ccgtcgccc gaacccgcg gaccaaacg 
      ttaacgaaa acgcagaaa aagcccaaa gtgcagaag 
      aagaaaaag aagcgggac cccaacgag ccccaaaaa 
      cccgtctcg gcttacgcc ttgttcttc cgtgatacg 
      caagcggcc atcaaggga caaaacccg aacgcgagt 
      ttcggggaa gtctcgaaa atcgttgct tccatgtgg 
      gacgctttg gactcggaa cataaaaac gtgtataaa 
      aagaagacc gaagcggcc aagaaagaa tacctgaaa 
      gcgctcgcc gcctaccgg gccagttta gtgtcgaaa 
      ggcgccggc gagggcgac ggcatgtac ggcaactac 
      ggcagttac aacgccggc gccccccaa tacgggggc 
      tacaccccg caaggcgcc atcccgtcg ccccccatg 
      tcctcagcg gcgacgccg cccacccag cagcccccc 
      atcggcaag aagccggcc atgatgtcg caacagcaa 
      cagcaacaa cagcaccaa aacatgatg cagtcgcca 
      atgggccaa gtgcacatg cagcacatg cagcaacag 
      cacaacagc tacatgcaa caggttcct caacagcag 
      cacatgccc gtgtcgccg cagcagccg cacatgcag 
      cagcacgtc agtccgccc ccgatggtc tcgagttcg 
      ccgcccgtc tcggcggcg gcgccgccc ccggccacc 
      gccgcccag caggcccaa ggacagatg agacccccc 
      aactcgtgt atacggcac ggctgcccc aacccggcc 
      atcaacaac gccgagtgg gaggacgag tactgcagt 
      aatgagtgc gtcgtgagc cattgccgg gatgtattc 
      acaaactgg gtagcgaac aacaagaac caatcacaa 
      aatttttcg acagttaaa taaattgta gacgtaggg 
      attgttgca agttcgagt taagcaata ctgagagga 
      acagatagg atatttaag tttgtaatc ggtccgagt 
      gctgtactg ataaaatga tgtgtaata gtgagaggc 
      taggttaag cttgtaaat aaaatgata atcatttag 
      agagtgaga ttgtattgt gggaagcaa taggagcaa 
      tacgatttc atgaaatag gtatacaat acaagacat 
      tgtaatcct agcatatac acgttgtat attaattat 
      tgaggaaaa tttgttaat tcatgctct gtaaatgca 
      tgtcccttt ggaaatagt agttagata tgtccaaag 
      tacataaga aaactatat aaaaggaaa actatttta 
      gttcatagt taggtcttt gttgttacg gaacgttcc 
      ccatccccg gcgtggctg aacgagaga gagagtata 
      aataatcgt gttctctgt tccttgtac tgaattcct 
      tttatttgg atctgaccg atcatagtt tgtagtgta 
      caattattt ttatatcat tgaccaatc aaagttacg 
      cttttttgt cgatgtata taaattgca gttgttatt 
      ttattgtta ttgatgtaa taagcaagc aattcggtt 
      gcgtcaaca tccctagta ttaaatacg tggtgaagg 
      atgtatatt tgtgaatat tatgaacga cttgttgag 
      attcgaact gtttgaagc agtgatatt gttaatgcg 
      tttttattt ccttcccga ctgtttgag acagtttga 
      actgtgaga actgtatta taatacgtt taggtaaat 
      gcatattac gacttcaat acttggttc tcatcgttc 
      ctttggttg tttcgcgga acaatcggc gatcgttcg 
      ttcgttcat cgttgggtc gacggatat caaaatcac 
      gactattcc aatccttat ctctacatg agctttaaa 
      attattaag tacaattta tgtttcact attacattg 
      taaatatca tattttatc ctgacaagc acaccttat 
      actatgtaa tcaattaat atttaaaat aaatgaata 
      gattctaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY