Status
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Gene information
General information
TC034442
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC012711,TC012710
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 13530856..13549718
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034442
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05302

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality), potentially confused/crosscontaminated with : iB # (comma separated) pciB046 C02
    unlikely but possible
    Pupal morphology
    beetle stock: bad (increased lethality), potentially confused/crosscontaminated with : iB # (comma separated) pciB046 C02
    unlikely but possible
    Fertility
    tropharium complete not present - Number of animals showing the phenotype: 4
    ovary shape irregular - Number of animals showing the phenotype: 4

    vitellogenic egg chamber late not present - Number of animals showing the phenotype: 4
    egg chamber potentially ectopicallypresent - Number of animals showing the phenotype: 4

    Comment: The egg chambers seem to be restricted to the anterior of the ovarioles. The vitellarium is usually empty.
    vitellarium irregular - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection



    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - (19 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    Pupal morphology
    wings irregular - Number of animals showing the phenotype: 4

    wings blistered - Number of animals showing the phenotype: 2

    wings orientation irregular - Number of animals showing the phenotype: 4

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034442 RA
      MLYILPPPR TPNPPSPWE SILFSGGGG GSANGNTSW 
      KPPRVRLRP KEGSTGPQD DNMDTNRTP KSLSECYFA 
      GKGTALVLP AAEAQKSNR RTDAQGSNI RKHLQSIVD 
      LLRPEETLK MAVKLESVH ASRVRYLVI VSRMGKRGE 
      ESCLLGIDC NEKTTVGLV LKVMADTAI RLDGDGGFS 
      VSVCGRHHI FKPVSVQAM WSALQTLHK VSSKAREQN 
      YFQGGLTHD WVEYYEKRI ESDRSCLNE WHAMDNLES 
      KRPPSPDSI RTKPTEREE TERVIRTTL KEIMMSVDL 
      DEVTSKYIR TRLEEDLDM DLGEYKSFI DQEMLTILG 
      QMDAPTEIF DHVYLGSEW NASNYEELQ RNGVGHILN 
      VTREIDNFF PGTFNYFNV RVCDDEKTD LLKYWDDTF 
      KYITSAKQN GSKVLVHCK MGVSRSASV VIAYAMKAY 
      NWDFQTAMH HVKEKRTCI KPNTSFLSQ LETYQGILD 
      AMKNKEKLQ RSKSETNLK SPDNKPDKI LPGSEPTPI 
      VQALPRYVA LSGQDLRSL GTRPKSWSP DNQSKEAEK 
      SPVSVSLED LSQKSTSKE SIIKEKKTQ RHVLMPCDN 
      GESYSVSPN RIVHLTHTE IPSVKDRVS ELESNKEAD 
      KSPPMPPRV LKKEWDPGD DDKKTTSSC NSTSDSCDS 
      RTDSVVNNG QVWTSSTQV KNLSDSAVQ TSVKDPFSN 
      QLDRVFDRE EKKQQRISI VEVANPELR ECPSRQSSW 
      SSYDSAVAL GSQNEVALS RHSSWGSGD TRLLPSRNS 
      SWGSYDMKP RGPVHYHNE KGEKILHES GTSGIFPYD 
      KEDIPWHPG TVKRTKQKL EENNMTKSS SSETLFEYA 
      TEQDKQPVE AINRSLVVL SPSAISPTI KIPGDDSKS 
      KSLSRLSVS APEPSSMDL VPADCSLSK SASNVTNTT 
      PGIVRSLKK EFEAKTSTN PEPETGKNK VTSLPASPV 
      TLHQDKEKV DEMNFKNLL GLFENNKEA QVAKRSKSA 
      KALIPRTAR YSCVEVSKW KNDKMTERP TPVVRTAPP 
      CLVASVIAS ATKKQQQYG KSHPLARLP IKPRHNNTL 
      YNTM
    CDS sequence
    • >TC034442 RA
      atgttgtac attttgccg cccccgagg accccgaac 
      ccgccgtcc ccatgggag agcatcctg ttctccggc 
      ggcggcggc ggcagcgcc aacggaaac acttcgtgg 
      aagccccca agggtgcgt cttaggccc aaagaggga 
      tcgaccgga ccgcaagat gacaacatg gacacgaac 
      cggaccccg aaaagcctc agcgagtgt tacttcgcg 
      ggcaagggt acagccttg gtgctgccg gccgcggag 
      gcgcaaaaa tccaacaga cggacggac gcccaaggc 
      agcaacatc aggaaacat ctccagtcg atcgtcgat 
      cttttgcga ccggaggaa acgctcaag atggccgtc 
      aagttggaa agcgtccat gcgtctcga gtgcgctac 
      ctggtgatc gtctccagg atgggcaag cgcggcgag 
      gagtcgtgt cttttaggc atcgattgc aacgaaaaa 
      accaccgtg gggttggtg ctgaaggtt atggcggac 
      acggccata cgattggac ggcgacggg ggcttcagc 
      gtgagcgtt tgcggccgc caccacatc ttcaagccg 
      gtgtccgtc caggcgatg tggtcggcc ctgcaaacc 
      ttgcacaag gtcagttcg aaagcccga gaacagaat 
      tacttccaa ggtggtctg acccatgac tgggtcgaa 
      tattatgaa aaaagaatc gaatcggat aggtcttgt 
      ctcaacgaa tggcatgcg atggataat ctcgagtcc 
      aaaagaccg ccgtcgccc gattcaata aggactaaa 
      ccaacggaa cgggaggaa accgagcgg gtgataagg 
      acgaccttg aaggaaatt atgatgtct gtggacttg 
      gacgaagtt acgagtaaa tatataagg acgagattg 
      gaggaggat ctcgatatg gacttggga gagtacaag 
      agtttcatc gaccaggaa atgttgacg attttgggt 
      cagatggac gcaccgacg gagattttc gatcacgtt 
      tacttgggt tccgaatgg aacgcttca aactacgaa 
      gagttacaa aggaacggc gttggccat atactgaac 
      gtgacccga gaaatcgac aatttcttc cctggcacc 
      ttcaattat ttcaacgtt cgcgtttgt gacgacgaa 
      aaaaccgac ttattgaaa tactgggac gacacattc 
      aaatacatc acgagcgcg aaacagaac ggttcgaaa 
      gtcctcgtc cattgcaaa atgggcgtg agtcgatcg 
      gcatcggtc gtaatcgcg tacgccatg aaagcgtac 
      aattgggac ttccaaacg gcaatgcat cacgtcaaa 
      gagaaaaga acgtgcata aagccgaac acgagtttc 
      ttgtcgcaa ttggagacc tatcagggc atcttggac 
      gcgatgaaa aacaaggaa aaactacag agatccaag 
      tctgaaacc aacttgaaa tcgccggat aataaaccg 
      gacaagatt cttccgggc agcgagccg actcccata 
      gtccaagcc ttgccgaga tacgttgct ctctccggc 
      caagacttg aggagttta ggcacgcgg ccgaaatcc 
      tggtcgccg gataaccag tcgaaagaa gcggaaaaa 
      tctccggtt tcggtctct ttggaggat ctcagtcaa 
      aaatccact tccaaagag agcatcatc aaggagaaa 
      aagactcaa cgccacgta ttaatgccc tgcgataac 
      ggggaatcg tacagtgtg tccccgaat cgcatcgtg 
      cacctcacc cacacggaa attccgtcg gtgaaggac 
      cgcgtcagc gagctcgaa tcgaacaaa gaagcggac 
      aagtcgccc ccgatgcca ccacgcgtc ttgaaaaaa 
      gagtgggac cccggcgat gacgacaaa aagacgacg 
      tcttcctgc aacagtaca agtgattcc tgtgattcc 
      cggactgat agtgtagtg aacaacggt caagtgtgg 
      actagttcg actcaagtg aaaaacctc tcggatagt 
      gctgtgcaa actagtgtt aaggatccg ttttcgaat 
      caactggac cgggttttc gaccgcgag gagaagaaa 
      caacaaagg atctcaatc gtcgaagtg gcgaaccct 
      gagttgagg gaatgtccg tcgagacag agttcgtgg 
      agttcgtac gacagtgcg gtggcgctg gggtcgcag 
      aacgaggtc gcgttgtca aggcatagc tcgtggggg 
      tcgggagat acgcgcttg ttaccttcg aggaatagc 
      tcctggggc tcgtacgac atgaagccg agaggcccc 
      gtacattat cacaacgaa aagggggag aagatttta 
      cacgaaagt ggcacttcg gggattttt ccctacgat 
      aaagaggac attccttgg catccggga acggtcaaa 
      aggacaaaa caaaaattg gaggaaaac aacatgaca 
      aaatcatcc tcgtcggaa accctcttc gaatacgcg 
      accgaacaa gacaagcaa ccggtggaa gcgatcaat 
      cgaagtctg gtagtcttg tctccgagt gccatatca 
      ccgactatc aaaattcca ggcgatgac agcaaaagc 
      aagagtttg tcgcggttg tccgtcagc gctccagag 
      ccgtcatca atggacttg gtccctgcg gactgttct 
      ctctccaaa tcggcttca aacgtcaca aatacaacc 
      cccggaatc gtccggagt cttaagaaa gaattcgaa 
      gcgaaaacc agcacaaac cctgagccg gaaacggga 
      aagaacaaa gtgacgagt ctgccggcg tcgcctgta 
      accctccac caggacaag gagaaagtc gacgaaatg 
      aactttaag aatttgtta ggtttgttc gagaacaac 
      aaagaagca caagtggcc aagcgctcg aaaagcgcg 
      aaagcgttg attccgcgg accgcgcgc tactcgtgc 
      gttgaagtc tccaagtgg aagaacgac aaaatgacg 
      gaaagaccg acgccggtg gtgcggacg gcgcccccg 
      tgtcttgtg gcgtcggtg attgcgagc gccacaaag 
      aaacagcag cagtacggc aaaagccat ccgcttgcc 
      cggctgccg atcaaacca aggcacaat aatacactt 
      tacaacact atgtaa
    mRNA sequence
    • >TC034442 RA
      tcagtttgc tgcaagcag tctcggtgc gagtgtcgt 
      gaaaaaacg gttaaacaa ccccattgt acgcaatgt 
      cttggtgat tcgtgtgag caatgttgt acattttgc 
      cgcccccga ggaccccga acccgccgt ccccatggg 
      agagcatcc tgttctccg gcggcggcg gcggcagcg 
      ccaacggaa acacttcgt ggaagcccc caagggtgc 
      gtcttaggc ccaaagagg gatcgaccg gaccgcaag 
      atgacaaca tggacacga accggaccc cgaaaagcc 
      tcagcgagt gttacttcg cgggcaagg gtacagcct 
      tggtgctgc cggccgcgg aggcgcaaa aatccaaca 
      gacggacgg acgcccaag gcagcaaca tcaggaaac 
      atctccagt cgatcgtcg atcttttgc gaccggagg 
      aaacgctca agatggccg tcaagttgg aaagcgtcc 
      atgcgtctc gagtgcgct acctggtga tcgtctcca 
      ggatgggca agcgcggcg aggagtcgt gtcttttag 
      gcatcgatt gcaacgaaa aaaccaccg tggggttgg 
      tgctgaagg ttatggcgg acacggcca tacgattgg 
      acggcgacg ggggcttca gcgtgagcg tttgcggcc 
      gccaccaca tcttcaagc cggtgtccg tccaggcga 
      tgtggtcgg ccctgcaaa ccttgcaca aggtcagtt 
      cgaaagccc gagaacaga attacttcc aaggtggtc 
      tgacccatg actgggtcg aatattatg aaaaaagaa 
      tcgaatcgg ataggtctt gtctcaacg aatggcatg 
      cgatggata atctcgagt ccaaaagac cgccgtcgc 
      ccgattcaa taaggacta aaccaacgg aacgggagg 
      aaaccgagc gggtgataa ggacgacct tgaaggaaa 
      ttatgatgt ctgtggact tggacgaag ttacgagta 
      aatatataa ggacgagat tggaggagg atctcgata 
      tggacttgg gagagtaca agagtttca tcgaccagg 
      aaatgttga cgattttgg gtcagatgg acgcaccga 
      cggagattt tcgatcacg tttacttgg gttccgaat 
      ggaacgctt caaactacg aagagttac aaaggaacg 
      gcgttggcc atatactga acgtgaccc gagaaatcg 
      acaatttct tccctggca ccttcaatt atttcaacg 
      ttcgcgttt gtgacgacg aaaaaaccg acttattga 
      aatactggg acgacacat tcaaataca tcacgagcg 
      cgaaacaga acggttcga aagtcctcg tccattgca 
      aaatgggcg tgagtcgat cggcatcgg tcgtaatcg 
      cgtacgcca tgaaagcgt acaattggg acttccaaa 
      cggcaatgc atcacgtca aagagaaaa gaacgtgca 
      taaagccga acacgagtt tcttgtcgc aattggaga 
      cctatcagg gcatcttgg acgcgatga aaaacaagg 
      aaaaactac agagatcca agtctgaaa ccaacttga 
      aatcgccgg ataataaac cggacaaga ttcttccgg 
      gcagcgagc cgactccca tagtccaag ccttgccga 
      gatacgttg ctctctccg gccaagact tgaggagtt 
      taggcacgc ggccgaaat cctggtcgc cggataacc 
      agtcgaaag aagcggaaa aatctccgg tttcggtct 
      ctttggagg atctcagtc aaaaatcca cttccaaag 
      agagcatca tcaaggaga aaaagactc aacgccacg 
      tattaatgc cctgcgata acggggaat cgtacagtg 
      tgtccccga atcgcatcg tgcacctca cccacacgg 
      aaattccgt cggtgaagg accgcgtca gcgagctcg 
      aatcgaaca aagaagcgg acaagtcgc ccccgatgc 
      caccacgcg tcttgaaaa aagagtggg accccggcg 
      atgacgaca aaaagacga cgtcttcct gcaacagta 
      caagtgatt cctgtgatt cccggactg atagtgtag 
      tgaacaacg gtcaagtgt ggactagtt cgactcaag 
      tgaaaaacc tctcggata gtgctgtgc aaactagtg 
      ttaaggatc cgttttcga atcaactgg accgggttt 
      tcgaccgcg aggagaaga aacaacaaa ggatctcaa 
      tcgtcgaag tggcgaacc ctgagttga gggaatgtc 
      cgtcgagac agagttcgt ggagttcgt acgacagtg 
      cggtggcgc tggggtcgc agaacgagg tcgcgttgt 
      caaggcata gctcgtggg ggtcgggag atacgcgct 
      tgttacctt cgaggaata gctcctggg gctcgtacg 
      acatgaagc cgagaggcc ccgtacatt atcacaacg 
      aaaaggggg agaagattt tacacgaaa gtggcactt 
      cggggattt ttccctacg ataaagagg acattcctt 
      ggcatccgg gaacggtca aaaggacaa aacaaaaat 
      tggaggaaa acaacatga caaaatcat cctcgtcgg 
      aaaccctct tcgaatacg cgaccgaac aagacaagc 
      aaccggtgg aagcgatca atcgaagtc tggtagtct 
      tgtctccga gtgccatat caccgacta tcaaaattc 
      caggcgatg acagcaaaa gcaagagtt tgtcgcggt 
      tgtccgtca gcgctccag agccgtcat caatggact 
      tggtccctg cggactgtt ctctctcca aatcggctt 
      caaacgtca caaatacaa cccccggaa tcgtccgga 
      gtcttaaga aagaattcg aagcgaaaa ccagcacaa 
      accctgagc cggaaacgg gaaagaaca aagtgacga 
      gtctgccgg cgtcgcctg taaccctcc accaggaca 
      aggagaaag tcgacgaaa tgaacttta agaatttgt 
      taggtttgt tcgagaaca acaaagaag cacaagtgg 
      ccaagcgct cgaaaagcg cgaaagcgt tgattccgc 
      ggaccgcgc gctactcgt gcgttgaag tctccaagt 
      ggaagaacg acaaaatga cggaaagac cgacgccgg 
      tggtgcgga cggcgcccc cgtgtcttg tggcgtcgg 
      tgattgcga gcgccacaa agaaacagc agcagtacg 
      gcaaaagcc atccgcttg cccggctgc cgatcaaac 
      caaggcaca ataatacac tttacaaca ctatgtaat 
      gctcaaatt acaaaaaaa tacggctgt gactgcact 
      cttgcgttt gtaattaag tgcttggaa ttttttctc 
      ccctcgctt gtctctctt tatctctgt atctctctg 
      aactgctct cggatagtg caaagtaaa tttaagtgt 
      taattgcgc ttgatgtaa ataggctat ttttcgaca 
      caaattgta aatttaagt ttcactgcc aaaaccaac 
      tgtggacca ttcataaaa caataagag caagagagg 
      ttccccgat ttcgtttca acatcacaa ttttggctg 
      tctaaaaat ctagtccct acagccccc tgtgaaacc 
      ccaagaaaa gctcaagcc tcgg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY