Status
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Gene information
General information
TC034442
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC012711,TC012710 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 13530856..13549718
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC034442
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05302

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    beetle stock: bad (increased lethality), potentially confused/crosscontaminated with : iB # (comma separated) pciB046 C02
    unlikely but possible
    Pupal morphology
    beetle stock: bad (increased lethality), potentially confused/crosscontaminated with : iB # (comma separated) pciB046 C02
    unlikely but possible
    Fertility
    tropharium complete not present - Number of animals showing the phenotype: 4
    ovary shape irregular - Number of animals showing the phenotype: 4

    vitellogenic egg chamber late not present - Number of animals showing the phenotype: 4
    egg chamber potentially ectopicallypresent - Number of animals showing the phenotype: 4

    Comment: The egg chambers seem to be restricted to the anterior of the ovarioles. The vitellarium is usually empty.
    vitellarium irregular - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    pupal molt not fulfilled (died as prepupa) - (19 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection

    Pupal morphology
    wings irregular - Number of animals showing the phenotype: 4

    wings blistered - Number of animals showing the phenotype: 2

    wings orientation irregular - Number of animals showing the phenotype: 4

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034442 RA
      MLYILPPPR TPNPPSPWE SILFSGGGG GSANGNTSW 
KPPRVRLRP KEGSTGPQD DNMDTNRTP KSLSECYFA 
GKGTALVLP AAEAQKSNR RTDAQGSNI RKHLQSIVD 
LLRPEETLK MAVKLESVH ASRVRYLVI VSRMGKRGE 
ESCLLGIDC NEKTTVGLV LKVMADTAI RLDGDGGFS 
VSVCGRHHI FKPVSVQAM WSALQTLHK VSSKAREQN 
YFQGGLTHD WVEYYEKRI ESDRSCLNE WHAMDNLES 
KRPPSPDSI RTKPTEREE TERVIRTTL KEIMMSVDL 
DEVTSKYIR TRLEEDLDM DLGEYKSFI DQEMLTILG 
QMDAPTEIF DHVYLGSEW NASNYEELQ RNGVGHILN 
VTREIDNFF PGTFNYFNV RVCDDEKTD LLKYWDDTF 
KYITSAKQN GSKVLVHCK MGVSRSASV VIAYAMKAY 
NWDFQTAMH HVKEKRTCI KPNTSFLSQ LETYQGILD 
AMKNKEKLQ RSKSETNLK SPDNKPDKI LPGSEPTPI 
VQALPRYVA LSGQDLRSL GTRPKSWSP DNQSKEAEK 
SPVSVSLED LSQKSTSKE SIIKEKKTQ RHVLMPCDN 
GESYSVSPN RIVHLTHTE IPSVKDRVS ELESNKEAD 
KSPPMPPRV LKKEWDPGD DDKKTTSSC NSTSDSCDS 
RTDSVVNNG QVWTSSTQV KNLSDSAVQ TSVKDPFSN 
QLDRVFDRE EKKQQRISI VEVANPELR ECPSRQSSW 
SSYDSAVAL GSQNEVALS RHSSWGSGD TRLLPSRNS 
SWGSYDMKP RGPVHYHNE KGEKILHES GTSGIFPYD 
KEDIPWHPG TVKRTKQKL EENNMTKSS SSETLFEYA 
TEQDKQPVE AINRSLVVL SPSAISPTI KIPGDDSKS 
KSLSRLSVS APEPSSMDL VPADCSLSK SASNVTNTT 
PGIVRSLKK EFEAKTSTN PEPETGKNK VTSLPASPV 
TLHQDKEKV DEMNFKNLL GLFENNKEA QVAKRSKSA 
KALIPRTAR YSCVEVSKW KNDKMTERP TPVVRTAPP 
CLVASVIAS ATKKQQQYG KSHPLARLP IKPRHNNTL 
YNTM
    CDS sequence
    • >TC034442 RA
      atgttgtac attttgccg cccccgagg accccgaac 
ccgccgtcc ccatgggag agcatcctg ttctccggc 
ggcggcggc ggcagcgcc aacggaaac acttcgtgg 
aagccccca agggtgcgt cttaggccc aaagaggga 
tcgaccgga ccgcaagat gacaacatg gacacgaac 
cggaccccg aaaagcctc agcgagtgt tacttcgcg 
ggcaagggt acagccttg gtgctgccg gccgcggag 
gcgcaaaaa tccaacaga cggacggac gcccaaggc 
agcaacatc aggaaacat ctccagtcg atcgtcgat 
cttttgcga ccggaggaa acgctcaag atggccgtc 
aagttggaa agcgtccat gcgtctcga gtgcgctac 
ctggtgatc gtctccagg atgggcaag cgcggcgag 
gagtcgtgt cttttaggc atcgattgc aacgaaaaa 
accaccgtg gggttggtg ctgaaggtt atggcggac 
acggccata cgattggac ggcgacggg ggcttcagc 
gtgagcgtt tgcggccgc caccacatc ttcaagccg 
gtgtccgtc caggcgatg tggtcggcc ctgcaaacc 
ttgcacaag gtcagttcg aaagcccga gaacagaat 
tacttccaa ggtggtctg acccatgac tgggtcgaa 
tattatgaa aaaagaatc gaatcggat aggtcttgt 
ctcaacgaa tggcatgcg atggataat ctcgagtcc 
aaaagaccg ccgtcgccc gattcaata aggactaaa 
ccaacggaa cgggaggaa accgagcgg gtgataagg 
acgaccttg aaggaaatt atgatgtct gtggacttg 
gacgaagtt acgagtaaa tatataagg acgagattg 
gaggaggat ctcgatatg gacttggga gagtacaag 
agtttcatc gaccaggaa atgttgacg attttgggt 
cagatggac gcaccgacg gagattttc gatcacgtt 
tacttgggt tccgaatgg aacgcttca aactacgaa 
gagttacaa aggaacggc gttggccat atactgaac 
gtgacccga gaaatcgac aatttcttc cctggcacc 
ttcaattat ttcaacgtt cgcgtttgt gacgacgaa 
aaaaccgac ttattgaaa tactgggac gacacattc 
aaatacatc acgagcgcg aaacagaac ggttcgaaa 
gtcctcgtc cattgcaaa atgggcgtg agtcgatcg 
gcatcggtc gtaatcgcg tacgccatg aaagcgtac 
aattgggac ttccaaacg gcaatgcat cacgtcaaa 
gagaaaaga acgtgcata aagccgaac acgagtttc 
ttgtcgcaa ttggagacc tatcagggc atcttggac 
gcgatgaaa aacaaggaa aaactacag agatccaag 
tctgaaacc aacttgaaa tcgccggat aataaaccg 
gacaagatt cttccgggc agcgagccg actcccata 
gtccaagcc ttgccgaga tacgttgct ctctccggc 
caagacttg aggagttta ggcacgcgg ccgaaatcc 
tggtcgccg gataaccag tcgaaagaa gcggaaaaa 
tctccggtt tcggtctct ttggaggat ctcagtcaa 
aaatccact tccaaagag agcatcatc aaggagaaa 
aagactcaa cgccacgta ttaatgccc tgcgataac 
ggggaatcg tacagtgtg tccccgaat cgcatcgtg 
cacctcacc cacacggaa attccgtcg gtgaaggac 
cgcgtcagc gagctcgaa tcgaacaaa gaagcggac 
aagtcgccc ccgatgcca ccacgcgtc ttgaaaaaa 
gagtgggac cccggcgat gacgacaaa aagacgacg 
tcttcctgc aacagtaca agtgattcc tgtgattcc 
cggactgat agtgtagtg aacaacggt caagtgtgg 
actagttcg actcaagtg aaaaacctc tcggatagt 
gctgtgcaa actagtgtt aaggatccg ttttcgaat 
caactggac cgggttttc gaccgcgag gagaagaaa 
caacaaagg atctcaatc gtcgaagtg gcgaaccct 
gagttgagg gaatgtccg tcgagacag agttcgtgg 
agttcgtac gacagtgcg gtggcgctg gggtcgcag 
aacgaggtc gcgttgtca aggcatagc tcgtggggg 
tcgggagat acgcgcttg ttaccttcg aggaatagc 
tcctggggc tcgtacgac atgaagccg agaggcccc 
gtacattat cacaacgaa aagggggag aagatttta 
cacgaaagt ggcacttcg gggattttt ccctacgat 
aaagaggac attccttgg catccggga acggtcaaa 
aggacaaaa caaaaattg gaggaaaac aacatgaca 
aaatcatcc tcgtcggaa accctcttc gaatacgcg 
accgaacaa gacaagcaa ccggtggaa gcgatcaat 
cgaagtctg gtagtcttg tctccgagt gccatatca 
ccgactatc aaaattcca ggcgatgac agcaaaagc 
aagagtttg tcgcggttg tccgtcagc gctccagag 
ccgtcatca atggacttg gtccctgcg gactgttct 
ctctccaaa tcggcttca aacgtcaca aatacaacc 
cccggaatc gtccggagt cttaagaaa gaattcgaa 
gcgaaaacc agcacaaac cctgagccg gaaacggga 
aagaacaaa gtgacgagt ctgccggcg tcgcctgta 
accctccac caggacaag gagaaagtc gacgaaatg 
aactttaag aatttgtta ggtttgttc gagaacaac 
aaagaagca caagtggcc aagcgctcg aaaagcgcg 
aaagcgttg attccgcgg accgcgcgc tactcgtgc 
gttgaagtc tccaagtgg aagaacgac aaaatgacg 
gaaagaccg acgccggtg gtgcggacg gcgcccccg 
tgtcttgtg gcgtcggtg attgcgagc gccacaaag 
aaacagcag cagtacggc aaaagccat ccgcttgcc 
cggctgccg atcaaacca aggcacaat aatacactt 
tacaacact atgtaa
    mRNA sequence
    • >TC034442 RA
      tcagtttgc tgcaagcag tctcggtgc gagtgtcgt 
gaaaaaacg gttaaacaa ccccattgt acgcaatgt 
cttggtgat tcgtgtgag caatgttgt acattttgc 
cgcccccga ggaccccga acccgccgt ccccatggg 
agagcatcc tgttctccg gcggcggcg gcggcagcg 
ccaacggaa acacttcgt ggaagcccc caagggtgc 
gtcttaggc ccaaagagg gatcgaccg gaccgcaag 
atgacaaca tggacacga accggaccc cgaaaagcc 
tcagcgagt gttacttcg cgggcaagg gtacagcct 
tggtgctgc cggccgcgg aggcgcaaa aatccaaca 
gacggacgg acgcccaag gcagcaaca tcaggaaac 
atctccagt cgatcgtcg atcttttgc gaccggagg 
aaacgctca agatggccg tcaagttgg aaagcgtcc 
atgcgtctc gagtgcgct acctggtga tcgtctcca 
ggatgggca agcgcggcg aggagtcgt gtcttttag 
gcatcgatt gcaacgaaa aaaccaccg tggggttgg 
tgctgaagg ttatggcgg acacggcca tacgattgg 
acggcgacg ggggcttca gcgtgagcg tttgcggcc 
gccaccaca tcttcaagc cggtgtccg tccaggcga 
tgtggtcgg ccctgcaaa ccttgcaca aggtcagtt 
cgaaagccc gagaacaga attacttcc aaggtggtc 
tgacccatg actgggtcg aatattatg aaaaaagaa 
tcgaatcgg ataggtctt gtctcaacg aatggcatg 
cgatggata atctcgagt ccaaaagac cgccgtcgc 
ccgattcaa taaggacta aaccaacgg aacgggagg 
aaaccgagc gggtgataa ggacgacct tgaaggaaa 
ttatgatgt ctgtggact tggacgaag ttacgagta 
aatatataa ggacgagat tggaggagg atctcgata 
tggacttgg gagagtaca agagtttca tcgaccagg 
aaatgttga cgattttgg gtcagatgg acgcaccga 
cggagattt tcgatcacg tttacttgg gttccgaat 
ggaacgctt caaactacg aagagttac aaaggaacg 
gcgttggcc atatactga acgtgaccc gagaaatcg 
acaatttct tccctggca ccttcaatt atttcaacg 
ttcgcgttt gtgacgacg aaaaaaccg acttattga 
aatactggg acgacacat tcaaataca tcacgagcg 
cgaaacaga acggttcga aagtcctcg tccattgca 
aaatgggcg tgagtcgat cggcatcgg tcgtaatcg 
cgtacgcca tgaaagcgt acaattggg acttccaaa 
cggcaatgc atcacgtca aagagaaaa gaacgtgca 
taaagccga acacgagtt tcttgtcgc aattggaga 
cctatcagg gcatcttgg acgcgatga aaaacaagg 
aaaaactac agagatcca agtctgaaa ccaacttga 
aatcgccgg ataataaac cggacaaga ttcttccgg 
gcagcgagc cgactccca tagtccaag ccttgccga 
gatacgttg ctctctccg gccaagact tgaggagtt 
taggcacgc ggccgaaat cctggtcgc cggataacc 
agtcgaaag aagcggaaa aatctccgg tttcggtct 
ctttggagg atctcagtc aaaaatcca cttccaaag 
agagcatca tcaaggaga aaaagactc aacgccacg 
tattaatgc cctgcgata acggggaat cgtacagtg 
tgtccccga atcgcatcg tgcacctca cccacacgg 
aaattccgt cggtgaagg accgcgtca gcgagctcg 
aatcgaaca aagaagcgg acaagtcgc ccccgatgc 
caccacgcg tcttgaaaa aagagtggg accccggcg 
atgacgaca aaaagacga cgtcttcct gcaacagta 
caagtgatt cctgtgatt cccggactg atagtgtag 
tgaacaacg gtcaagtgt ggactagtt cgactcaag 
tgaaaaacc tctcggata gtgctgtgc aaactagtg 
ttaaggatc cgttttcga atcaactgg accgggttt 
tcgaccgcg aggagaaga aacaacaaa ggatctcaa 
tcgtcgaag tggcgaacc ctgagttga gggaatgtc 
cgtcgagac agagttcgt ggagttcgt acgacagtg 
cggtggcgc tggggtcgc agaacgagg tcgcgttgt 
caaggcata gctcgtggg ggtcgggag atacgcgct 
tgttacctt cgaggaata gctcctggg gctcgtacg 
acatgaagc cgagaggcc ccgtacatt atcacaacg 
aaaaggggg agaagattt tacacgaaa gtggcactt 
cggggattt ttccctacg ataaagagg acattcctt 
ggcatccgg gaacggtca aaaggacaa aacaaaaat 
tggaggaaa acaacatga caaaatcat cctcgtcgg 
aaaccctct tcgaatacg cgaccgaac aagacaagc 
aaccggtgg aagcgatca atcgaagtc tggtagtct 
tgtctccga gtgccatat caccgacta tcaaaattc 
caggcgatg acagcaaaa gcaagagtt tgtcgcggt 
tgtccgtca gcgctccag agccgtcat caatggact 
tggtccctg cggactgtt ctctctcca aatcggctt 
caaacgtca caaatacaa cccccggaa tcgtccgga 
gtcttaaga aagaattcg aagcgaaaa ccagcacaa 
accctgagc cggaaacgg gaaagaaca aagtgacga 
gtctgccgg cgtcgcctg taaccctcc accaggaca 
aggagaaag tcgacgaaa tgaacttta agaatttgt 
taggtttgt tcgagaaca acaaagaag cacaagtgg 
ccaagcgct cgaaaagcg cgaaagcgt tgattccgc 
ggaccgcgc gctactcgt gcgttgaag tctccaagt 
ggaagaacg acaaaatga cggaaagac cgacgccgg 
tggtgcgga cggcgcccc cgtgtcttg tggcgtcgg 
tgattgcga gcgccacaa agaaacagc agcagtacg 
gcaaaagcc atccgcttg cccggctgc cgatcaaac 
caaggcaca ataatacac tttacaaca ctatgtaat 
gctcaaatt acaaaaaaa tacggctgt gactgcact 
cttgcgttt gtaattaag tgcttggaa ttttttctc 
ccctcgctt gtctctctt tatctctgt atctctctg 
aactgctct cggatagtg caaagtaaa tttaagtgt 
taattgcgc ttgatgtaa ataggctat ttttcgaca 
caaattgta aatttaagt ttcactgcc aaaaccaac 
tgtggacca ttcataaaa caataagag caagagagg 
ttccccgat ttcgtttca acatcacaa ttttggctg 
tctaaaaat ctagtccct acagccccc tgtgaaacc 
ccaagaaaa gctcaagcc tcgg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY