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Gene information
General information
TC034436 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 13406315..13408723
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034436
    iBeetle screen iB_05300

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    posterior terminus sometimes partiallyremaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: Labium and T1 might be the most often affected segments.
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Comment: Labium and T1 might be the most often affected segments.
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Comment: Labium and T1 might be the most often affected segments.
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034436 RA
      MRVSLLGFV LVVCVVRQS WGYKELSME NDELCSPIN 
      KKRRVRLDK GHRSGLVVR QFRLQDHSL DWTRNFKCR 
      FEVTPSSSR EGVIAVIQH LSFRKNAST DECVDYVQF 
      TRKDGSSSQ KYCGRFNAA LYMDHNFVN TADPISSGT 
      AFVDDSGEL EVAISVSRE HLENDEEMD LSIVFTAFR 
      HCSQVLKRD TSYRPCSEK RREYCIYSG FFMDSFVNC 
      PFPDCEDEQ GCSQTPIEV VNVYYGNKV LIGAVSSVF 
      VVFALFILC LWICKSYKI FCWSQSFAN PASSSQNPS 
      DAPRVIELN EQTGSRASA PPPNASSAN QIEADKDLP 
      PSYESLFPS R
    CDS sequence
    • >TC034436 RA
      atgagagtg tctttgctg ggtttcgtg ttggtcgtg 
      tgcgtggtg cggcagtcg tgggggtac aaggagctg 
      agcatggag aacgacgag ttgtgttcg cctataaac 
      aagaaacgt cgggtccga ctggacaag gggcaccgg 
      tcggggctc gtggtgcgc cagttccgg ctgcaggac 
      cacagttta gactggact aggaacttc aaatgtcgg 
      ttcgaagtc actccttcg agctcgcgc gagggcgta 
      atagctgtg atacaacac ttgagtttt cgcaagaat 
      gcctccacg gatgagtgc gtggactac gtgcaattc 
      acgcggaaa gacggctcc tcgagccag aagtactgt 
      gggcggttc aacgccgcc ctctacatg gaccacaac 
      ttcgtgaat acggccgat cccatctcg tccgggacg 
      gcgttcgtg gacgacagc ggggagctg gaggtggcc 
      atctcggtg tcgcgggag cacctggag aacgacgag 
      gagatggat ttgagcatc gtcttcaca gcgtttagg 
      cattgttca caagtcctg aagcgagac acgagttac 
      aggccgtgt agcgagaaa cggcgcgag tattgcatt 
      tattccggg tttttcatg gacagtttc gtcaattgt 
      ccgtttccg gactgcgag gatgagcaa ggctgctca 
      caaacgcca atcgaagtg gtcaatgtt tactatggc 
      aataaagtc ctgatcggg gctgtgtct tccgtcttt 
      gtggtgttt gcgcttttc atcctatgc ctgtggatc 
      tgcaagtcg tacaaaatt ttttgctgg tcgcaaagt 
      ttcgcaaat cccgcttct tcctcccaa aatccgagc 
      gatgcacct agggtaata gaattgaat gagcagacg 
      gggtctagg gcttcggcc cctccgccg aatgcgtct 
      agtgccaat caaatcgaa gcagataag gatttgcca 
      ccgtcgtat gaaagtctt tttccctcg aggtag
    mRNA sequence
    • >TC034436 RA
      ctgtcactt gaaattgta aatagtgtt ttaccaaac 
      aatgtaaga aaaatacaa tgagagtgt ctttgctgg 
      gtttcgtgt tggtcgtgt gcgtggtgc ggcagtcgt 
      gggggtaca aggagctga gcatggaga acgacgagt 
      tgtgttcgc ctataaaca agaaacgtc gggtccgac 
      tggacaagg ggcaccggt cggggctcg tggtgcgcc 
      agttccggc tgcaggacc acagtttag actggacta 
      ggaacttca aatgtcggt tcgaagtca ctccttcga 
      gctcgcgcg agggcgtaa tagctgtga tacaacact 
      tgagttttc gcaagaatg cctccacgg atgagtgcg 
      tggactacg tgcaattca cgcggaaag acggctcct 
      cgagccaga agtactgtg ggcggttca acgccgccc 
      tctacatgg accacaact tcgtgaata cggccgatc 
      ccatctcgt ccgggacgg cgttcgtgg acgacagcg 
      gggagctgg aggtggcca tctcggtgt cgcgggagc 
      acctggaga acgacgagg agatggatt tgagcatcg 
      tcttcacag cgtttaggc attgttcac aagtcctga 
      agcgagaca cgagttaca ggccgtgta gcgagaaac 
      ggcgcgagt attgcattt attccgggt ttttcatgg 
      acagtttcg tcaattgtc cgtttccgg actgcgagg 
      atgagcaag gctgctcac aaacgccaa tcgaagtgg 
      tcaatgttt actatggca ataaagtcc tgatcgggg 
      ctgtgtctt ccgtctttg tggtgtttg cgcttttca 
      tcctatgcc tgtggatct gcaagtcgt acaaaattt 
      tttgctggt cgcaaagtt tcgcaaatc ccgcttctt 
      cctcccaaa atccgagcg atgcaccta gggtaatag 
      aattgaatg agcagacgg ggtctaggg cttcggccc 
      ctccgccga atgcgtcta gtgccaatc aaatcgaag 
      cagataagg atttgccac cgtcgtatg aaagtcttt 
      ttccctcga ggtagtagt acaagaaac tgggagggg 
      gtacattcc acttagatt gctacgaaa gtgaattgt 
      tatcgattt gtgtaaaaa gacaatttt gtacaattt 
      atttactaa tactaacgc atgtcagta ttaaaatac 
      aagtctact tgccaaata ctgaatttt tatatacta 
      gaatgtttt tataactat tatgaattt ctatttata 
      ttttttatc aagttgtga aacaataaa cgattatgt 
      aatac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY