Status
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Gene information
General information
TC012700 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 13393625..13403229
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012700
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05299

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    head appendages random not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head appendages position irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    head shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012700 RA
      MASPQACLS SSPEIVSPG GKSRYGRTR RPKISHDFC 
      NIDDVIIDD NPPEVKSPL KARDVVFEI KDEFTKLDD 
      LGMKVANNF KTNRGLDAS IKERKFFKS AVVSKAVDV 
      VDVSKVLKL VSTPGAEAQ AKSPPKTVL KTYSNRRKH 
      AENERVVES FGLPDEPII PVKGHAFQR HSKIDVDFA 
      AENDVSLPN PWTFDEIFT NEMKGPTNG ETFDENTDF 
      QQTLTPKGA KNAFERTTG KKRLFRSSA EEGGTIVTV 
      SPVLKKAKV GSSPPKRVL RSNFESNVK IQSSVSPQN 
      ETNDSVVEA ELPSAGNES EEELPNISH KSLRSSTLK 
      NKSPEKRIE ISHEKNKIK TKSPEKCKP KKGLNLVKT 
      AKEISTETN KIRVGGTEC TVKNKSPKK SKTKEALNP 
      VVTVEEISP EKNNVQDDA AEENPLKNG SSGKSKTKE 
      ALNPVKMVK TSAEKNDGL EAVTKSALT MTQTKTKEV 
      FNPVVIVTE ISPEKNNVQ EDVAENRTE ESGPKNKPK 
      EVFLNPVII MKEFSAVKN DGPEAAESE PSMTQSKTK 
      EVLEISAET NQVPENETK EELKVIKQE VIELEVGVL 
      AWAKLGNFP YWPCLITQE PASGTHQKY VVNRIHPLY 
      HVRFFGDKG RRSWVHGPN VMPFYAKDD LERLAKTLE 
      VEGKFKPSY INECCFIRR TLMKKWQEG VAEAESLQF 
      KTVDERLNY FSKMFARKN ELKQERQKL KRARLSAAE 
      NLSKKVKIE EIETLELIK PEPVEVTSS PDTLIKPPK 
      KKNFLKKEE FSDEQLEAR KKLRNPQVR EEIPQPSSA 
      PKLRKKPQG ENSNSDTDS KFSDNSSLP PSMQSQAAL 
      FRRNNIFRG IPREKVCQI CEKSGEIFK CKGPCNGVY 
      HPECAMNRV VEQKSSVEI ISLESKQPL PDLGTSPNY 
      TGMSLAEKI DHRMKEIMK KFDQSVYCE STDSSSDEY 
      VVEEVVRQA SPPAQSKLS LTVTATPTK KEVPIIKHV 
      TADNISYIC VSNEAPKED TTGLKCTWC ASDEAPLCL 
      VCGEMGAQG RQKCSLHQC GRFYHPECL KLWPQTQWS 
      LNASDSFVC PRHVCHTCI SDDPRAANS RCSSDKIVK 
      CLKCPATYH SSNYCVPAG TEILTASQI ICPRHFTRN 
      KRNYQSTIN ANWCFICSN GGDLICCET CPTSVHREC 
      LPGDLGEVE TFFCEDCQS GRLPLYDEI VWVKLGSFR 
      WWPAVILFP NEVPDNVKN IPHSKGEFV VKFYGTYDH 
      YWVGRGRTF LFQEGDRGH SGSVKKRVD NAFVKAIEE 
      AAAAHELKK QFKARKFEE KNSGMKPPP YVRIKVNKP 
      VGNVRVFDG NTSNTTSCD CDPNQPHPC GPDSDCLNR 
      LLLTECNPD VCPAGDRCN NQCFEKREY PPLVPHRTL 
      YRGWGLKTL APIRKGQFV IEYVGEMID EQEYQRRVQ 
      KMHEQKEEN YYFLTIDKD RMLDAGPKG NVARFMNHS 
      CDPNCETQK WTVNGDTRV GLFANCDIP AGTELTFNY 
      NLECIGKEK KICHCGAPN CSGFIGVKV KTDNPPKKS 
      TKAKKKKTP PLEYVDNDN PCFICGKQG DVAACNNKI 
      CTKYYHLAC VGLEAWPEG NKWVCQWHN CNICTKRTI 
      RCCVRCINS YCPSHSDGN VRYDKLLGF VCSEHDSSK 
      ESNAVVTSH VPAPDSCVS DEGVVTSTT SDDSRMNGF 
      VTSTPVKKN DNSYDESVV SSSEKKPKK VKKKKVENT 
      ANFLLENEV KLNNFAPVK YLRTSRSRN SDPERDLPI 
      RKRTRLRCG SSL
    CDS sequence
    • >TC012700 RA
      atggcttcg cctcaagcc tgcctgagt tctagtccc 
      gaaatagtc agccccgga ggcaagtcc cgctatggg 
      cggaccagg cgccccaaa atctcgcac gacttttgc 
      aacatcgac gacgttatc atcgacgat aaccccccg 
      gaagtgaag tcgcccctc aaggcccgc gatgtcgtg 
      ttcgagatc aaggacgag tttacgaaa ctcgacgac 
      ttggggatg aaagtggcg aacaacttc aaaacgaac 
      cgaggcttg gatgcttcg atcaaagag cgcaagttt 
      ttcaaaagt gcggttgta agcaaggcg gtggatgtt 
      gttgacgtg agcaaggtg cttaagttg gtttcgacg 
      cccggtgcc gaggcccag gccaaaagc ccgccgaaa 
      accgtcctg aagacgtac tcaaatcgg cggaaacac 
      gccgagaac gagcgcgtt gttgagagt tttggtttg 
      ccggacgaa ccgatcatt ccagtgaaa ggccacgct 
      ttccagcga catagcaaa attgatgtc gattttgcg 
      gccgaaaac gacgtgagt ttgccgaat ccgtggacg 
      tttgatgag atttttacc aacgagatg aagggaccc 
      acgaatgga gaaaccttt gatgaaaac accgatttt 
      caacaaacg ctcaccccc aagggggcc aaaaacgcc 
      ttcgaacgt actacagga aagaagagg ttgttccgc 
      agttctgca gaggaaggg ggcacaata gttacagtt 
      tcaccagtg ttaaagaaa gccaaagtt gggagtagc 
      cccccgaag agggttctc aggtcgaat tttgagtca 
      aatgtgaaa atccagtcg tctgtttca ccacaaaat 
      gaaacaaat gatagtgta gtggaggca gagttgcca 
      agtgcgggc aatgaatca gaagaggag ttgccaaat 
      ataagtcac aagtcacta agatcaagc actctaaaa 
      aacaagtca cccgagaaa agaatagaa atttcccac 
      gaaaaaaat aaaataaaa accaagtca cctgaaaaa 
      tgtaagcca aaaaaaggc ttaaatctg gtaaaaaca 
      gcaaaagaa atttccacc gaaacaaat aaaatacgg 
      gttggtggg acggagtgt actgtaaaa aacaagtcg 
      cctaaaaaa agtaaaaca aaagaagcc ttaaatccc 
      gtagtaaca gtcgaagag atctctccc gaaaaaaat 
      aacgtacag gacgatgcg gcggaagaa aatcctcta 
      aaaaatggc tcgtctgga aaaagtaaa acaaaggaa 
      gccttaaat ccggtaaaa atggtgaaa acttccgcc 
      gaaaaaaac gacggactt gaagccgtg acaaaaagc 
      gcgctgaca atgacacaa actaaaaca aaagaagtc 
      ttcaatcct gtagtaata gtgacagag atttctccc 
      gaaaagaat aacgtacag gaagatgtg gcagaaaac 
      aggacggag gaaagtggt ccaaaaaat aaaccaaaa 
      gaagttttt ttaaatcct gtaataatc atgaaagaa 
      ttttccgct gtaaaaaac gacggccct gaggctgca 
      gaaagcgag ccgtcaatg acacaaagt aaaacaaaa 
      gaagtctta gaaatttcc gccgaaaca aatcaagta 
      cccgaaaat gagacgaaa gaggaattg aaagttatt 
      aaacaagaa gtaatagag ctcgaagtt ggggttctg 
      gcctgggct aaacttggc aattttccc tactggccg 
      tgtctgatt acgcaagaa ccggcgagt ggaacgcat 
      caaaaatac gttgtgaat cggatccat ccgctttat 
      cacgtgaga tttttcggt gataaaggc cgaaggtca 
      tgggtgcat ggcccaaac gtgatgcct ttttacgcc 
      aaagacgat ctcgaacgt cttgcaaaa acgctcgaa 
      gtcgaggga aaatttaaa ccatcctat atcaatgaa 
      tgttgcttt ataagacgc accctaatg aagaaatgg 
      caagaagga gtcgctgaa gccgaatct ttacaattc 
      aaaacagtc gacgaacgc ttgaattac ttttcgaag 
      atgtttgcc agaaaaaac gaactgaag caagaaaga 
      caaaaactg aaacgtgcg cggttatca gccgccgaa 
      aacttgtcg aaaaaagtg aaaatcgaa gaaattgaa 
      acacttgaa ttgatcaaa ccggaacca gtcgaagtt 
      acttcgagc cccgacact ttaataaaa ccccccaaa 
      aagaagaat ttcttaaag aaggaagaa ttttccgac 
      gaacaactc gaagcgcgc aagaaacta cgcaatcca 
      caagtgcgg gaagaaatc ccccaacct tcgagcgcc 
      ccgaagttg cgtaaaaag ccccaaggt gaaaattca 
      aactcggac accgactcc aaattcagc gacaacagc 
      tcgctccca ccctccatg caatcgcag gcggccctg 
      tttcgccgc aacaacatc ttccgggga atccctcgc 
      gagaaagtg tgccaaata tgcgaaaaa tccggggaa 
      atcttcaaa tgcaagggc ccctgtaac ggcgtttac 
      caccccgag tgtgccatg aatcgcgtc gtggaacaa 
      aaaagcagc gtcgaaata atctcactc gaatcgaag 
      caacccttg cccgacctg ggtacttcc cccaactac 
      acgggaatg tccctagct gagaaaatc gaccatcgg 
      atgaaagaa ataatgaaa aagtttgac caatcggtt 
      tattgcgaa tcgaccgat tccagtagc gatgagtac 
      gttgtagaa gaggtggtt agacaagcc tcgccccca 
      gcccaatcg aaactctcc ctcaccgtc accgccacg 
      cccacgaag aaggaagta ccaatcata aagcacgtc 
      actgccgac aatatcagt tacatttgc gtgagtaat 
      gaggcacca aaggaggac accacgggt ttgaaatgc 
      acgtggtgt gcctcagac gaggccccc ttgtgcctc 
      gtgtgtggg gagatgggg gcccaaggg cgccagaag 
      tgctcgctg caccaatgc ggccgtttc taccacccc 
      gagtgcctc aaactgtgg ccgcagacg cagtggtcg 
      ctgaacgcg tcggacagc ttcgtgtgt cctcggcac 
      gtgtgccac acgtgcatt tccgacgat ccccgagcg 
      gcgaactcg cgctgttcg agcgataaa atcgtaaaa 
      tgcctcaaa tgccccgcc acctaccac tcgagcaat 
      tactgcgtg cctgcgggc acggaaatt ctgaccgcc 
      tcccagatt atatgcccg aggcatttt accagaaac 
      aagcgcaac taccaatca acgatcaac gccaattgg 
      tgtttcatt tgttcgaac gggggcgac ttgatctgc 
      tgcgagacg tgtccgacc agtgtgcac agggagtgt 
      cttccgggt gatttgggc gaggttgag acgtttttc 
      tgtgaggac tgccaatcg gggcggttg cccctctat 
      gatgagatc gtttgggtg aagctcggg tcgtttcgg 
      tggtggccg gccgtgatc ttgttcccg aatgaggtt 
      ccagataac gtgaaaaat atcccgcat agtaagggg 
      gagtttgtg gtcaagttt tacgggacg tacgaccat 
      tattgggtc gggagaggg aggacgttc ttgtttcag 
      gaaggcgat cggggacat tcgggttcg gtgaagaag 
      cgagtcgat aacgcgttc gttaaggcg attgaggag 
      gcggcagca gcgcacgag ttgaagaaa caatttaag 
      gcgaggaaa tttgaagag aagaatagt ggaatgaaa 
      ccgccaccg tacgttcgg attaaggtg aataaaccc 
      gtggggaat gtgagggtg tttgatggg aatacgagt 
      aatacgacc tcttgtgat tgtgatccg aaccagccg 
      catccgtgt ggacctgat agcgactgt ctaaacagg 
      ctgcttttg accgaatgc aaccccgac gtgtgcccg 
      gccggcgac cgttgcaac aaccaatgt tttgaaaaa 
      cgcgaatac ccccctttg gtgccccac cgcacactt 
      taccgcggt tggggcctc aaaaccctt gctccaatc 
      cgaaaaggc caattcgta atcgaatac gttggcgaa 
      atgatcgac gaacaggag taccaacgt cgcgtccaa 
      aaaatgcac gaacaaaaa gaagaaaac tactatttc 
      ctcacgatc gacaaggac aggatgttg gatgccggt 
      ccgaaaggc aacgttgcc agatttatg aaccattcg 
      tgtgacccg aactgtgag acacaaaaa tggacagtg 
      aatggcgac accagagtt ggattattc gcaaattgc 
      gacattcca gccggcact gagctcacc ttcaattac 
      aatttagaa tgcattggg aaagaaaag aagatctgt 
      cactgtggc gcccccaat tgtagtggc tttattggt 
      gttaaagtg aaaacggat aatcccccg aaaaagtcg 
      actaaagcc aagaagaag aaaacacca ccgcttgaa 
      tatgtcgat aatgacaat ccctgcttt atttgcggc 
      aagcaaggc gacgttgcc gcgtgcaat aataagatt 
      tgtacaaag tattatcat ttagcctgt gtggggctt 
      gaggcatgg ccggaaggg aacaagtgg gtgtgtcag 
      tggcacaat tgcaacatt tgcaccaag cgcacaatt 
      cgctgttgc gtgcgatgc attaactcg tattgtccg 
      tctcactct gacggcaac gtccgttac gataagctg 
      ctagggttc gtctgtagt gagcatgac tcgtcgaag 
      gagtccaac gcggtcgtg acgagccac gtgcccgca 
      cccgattcg tgcgtcagt gatgagggc gtggtcacg 
      tcgacgact tccgatgat tcacgaatg aacggattc 
      gtcacctcc acgccggtg aagaaaaac gacaattcg 
      tacgatgag agtgtggtg agttcgtcc gaaaagaaa 
      ccgaagaag gttaaaaag aagaaagtt gaaaacact 
      gccaatttt ttactcgaa aacgaagtc aagctgaat 
      aatttcgcg cccgtgaag tatttgagg actagtagg 
      agtaggaat agtgaccct gaaagggac ctcccgatt 
      cgaaaacgt accagactt agatgtgga agttcgttg 
      tag
    mRNA sequence
    • >TC012700 RA
      ccatccaaa aaatgaaaa agcgggaaa gtgcaaaaa 
      agaatcggc gccaacttc acgtcacaa catccaaaa 
      acttgcact aaaaaccga tccaaaaca aaaatattg 
      ataagttcc tattccact gtcgcccca aaatggttc 
      aaaattatt atcagggtc cacgtccag gcagttaaa 
      tggctgttg gcttgtgtg atggcttcg cctcaagcc 
      tgcctgagt tctagtccc gaaatagtc agccccgga 
      ggcaagtcc cgctatggg cggaccagg cgccccaaa 
      atctcgcac gacttttgc aacatcgac gacgttatc 
      atcgacgat aaccccccg gaagtgaag tcgcccctc 
      aaggcccgc gatgtcgtg ttcgagatc aaggacgag 
      tttacgaaa ctcgacgac ttggggatg aaagtggcg 
      aacaacttc aaaacgaac cgaggcttg gatgcttcg 
      atcaaagag cgcaagttt ttcaaaagt gcggttgta 
      agcaaggcg gtggatgtt gttgacgtg agcaaggtg 
      cttaagttg gtttcgacg cccggtgcc gaggcccag 
      gccaaaagc ccgccgaaa accgtcctg aagacgtac 
      tcaaatcgg cggaaacac gccgagaac gagcgcgtt 
      gttgagagt tttggtttg ccggacgaa ccgatcatt 
      ccagtgaaa ggccacgct ttccagcga catagcaaa 
      attgatgtc gattttgcg gccgaaaac gacgtgagt 
      ttgccgaat ccgtggacg tttgatgag atttttacc 
      aacgagatg aagggaccc acgaatgga gaaaccttt 
      gatgaaaac accgatttt caacaaacg ctcaccccc 
      aagggggcc aaaaacgcc ttcgaacgt actacagga 
      aagaagagg ttgttccgc agttctgca gaggaaggg 
      ggcacaata gttacagtt tcaccagtg ttaaagaaa 
      gccaaagtt gggagtagc cccccgaag agggttctc 
      aggtcgaat tttgagtca aatgtgaaa atccagtcg 
      tctgtttca ccacaaaat gaaacaaat gatagtgta 
      gtggaggca gagttgcca agtgcgggc aatgaatca 
      gaagaggag ttgccaaat ataagtcac aagtcacta 
      agatcaagc actctaaaa aacaagtca cccgagaaa 
      agaatagaa atttcccac gaaaaaaat aaaataaaa 
      accaagtca cctgaaaaa tgtaagcca aaaaaaggc 
      ttaaatctg gtaaaaaca gcaaaagaa atttccacc 
      gaaacaaat aaaatacgg gttggtggg acggagtgt 
      actgtaaaa aacaagtcg cctaaaaaa agtaaaaca 
      aaagaagcc ttaaatccc gtagtaaca gtcgaagag 
      atctctccc gaaaaaaat aacgtacag gacgatgcg 
      gcggaagaa aatcctcta aaaaatggc tcgtctgga 
      aaaagtaaa acaaaggaa gccttaaat ccggtaaaa 
      atggtgaaa acttccgcc gaaaaaaac gacggactt 
      gaagccgtg acaaaaagc gcgctgaca atgacacaa 
      actaaaaca aaagaagtc ttcaatcct gtagtaata 
      gtgacagag atttctccc gaaaagaat aacgtacag 
      gaagatgtg gcagaaaac aggacggag gaaagtggt 
      ccaaaaaat aaaccaaaa gaagttttt ttaaatcct 
      gtaataatc atgaaagaa ttttccgct gtaaaaaac 
      gacggccct gaggctgca gaaagcgag ccgtcaatg 
      acacaaagt aaaacaaaa gaagtctta gaaatttcc 
      gccgaaaca aatcaagta cccgaaaat gagacgaaa 
      gaggaattg aaagttatt aaacaagaa gtaatagag 
      ctcgaagtt ggggttctg gcctgggct aaacttggc 
      aattttccc tactggccg tgtctgatt acgcaagaa 
      ccggcgagt ggaacgcat caaaaatac gttgtgaat 
      cggatccat ccgctttat cacgtgaga tttttcggt 
      gataaaggc cgaaggtca tgggtgcat ggcccaaac 
      gtgatgcct ttttacgcc aaagacgat ctcgaacgt 
      cttgcaaaa acgctcgaa gtcgaggga aaatttaaa 
      ccatcctat atcaatgaa tgttgcttt ataagacgc 
      accctaatg aagaaatgg caagaagga gtcgctgaa 
      gccgaatct ttacaattc aaaacagtc gacgaacgc 
      ttgaattac ttttcgaag atgtttgcc agaaaaaac 
      gaactgaag caagaaaga caaaaactg aaacgtgcg 
      cggttatca gccgccgaa aacttgtcg aaaaaagtg 
      aaaatcgaa gaaattgaa acacttgaa ttgatcaaa 
      ccggaacca gtcgaagtt acttcgagc cccgacact 
      ttaataaaa ccccccaaa aagaagaat ttcttaaag 
      aaggaagaa ttttccgac gaacaactc gaagcgcgc 
      aagaaacta cgcaatcca caagtgcgg gaagaaatc 
      ccccaacct tcgagcgcc ccgaagttg cgtaaaaag 
      ccccaaggt gaaaattca aactcggac accgactcc 
      aaattcagc gacaacagc tcgctccca ccctccatg 
      caatcgcag gcggccctg tttcgccgc aacaacatc 
      ttccgggga atccctcgc gagaaagtg tgccaaata 
      tgcgaaaaa tccggggaa atcttcaaa tgcaagggc 
      ccctgtaac ggcgtttac caccccgag tgtgccatg 
      aatcgcgtc gtggaacaa aaaagcagc gtcgaaata 
      atctcactc gaatcgaag caacccttg cccgacctg 
      ggtacttcc cccaactac acgggaatg tccctagct 
      gagaaaatc gaccatcgg atgaaagaa ataatgaaa 
      aagtttgac caatcggtt tattgcgaa tcgaccgat 
      tccagtagc gatgagtac gttgtagaa gaggtggtt 
      agacaagcc tcgccccca gcccaatcg aaactctcc 
      ctcaccgtc accgccacg cccacgaag aaggaagta 
      ccaatcata aagcacgtc actgccgac aatatcagt 
      tacatttgc gtgagtaat gaggcacca aaggaggac 
      accacgggt ttgaaatgc acgtggtgt gcctcagac 
      gaggccccc ttgtgcctc gtgtgtggg gagatgggg 
      gcccaaggg cgccagaag tgctcgctg caccaatgc 
      ggccgtttc taccacccc gagtgcctc aaactgtgg 
      ccgcagacg cagtggtcg ctgaacgcg tcggacagc 
      ttcgtgtgt cctcggcac gtgtgccac acgtgcatt 
      tccgacgat ccccgagcg gcgaactcg cgctgttcg 
      agcgataaa atcgtaaaa tgcctcaaa tgccccgcc 
      acctaccac tcgagcaat tactgcgtg cctgcgggc 
      acggaaatt ctgaccgcc tcccagatt atatgcccg 
      aggcatttt accagaaac aagcgcaac taccaatca 
      acgatcaac gccaattgg tgtttcatt tgttcgaac 
      gggggcgac ttgatctgc tgcgagacg tgtccgacc 
      agtgtgcac agggagtgt cttccgggt gatttgggc 
      gaggttgag acgtttttc tgtgaggac tgccaatcg 
      gggcggttg cccctctat gatgagatc gtttgggtg 
      aagctcggg tcgtttcgg tggtggccg gccgtgatc 
      ttgttcccg aatgaggtt ccagataac gtgaaaaat 
      atcccgcat agtaagggg gagtttgtg gtcaagttt 
      tacgggacg tacgaccat tattgggtc gggagaggg 
      aggacgttc ttgtttcag gaaggcgat cggggacat 
      tcgggttcg gtgaagaag cgagtcgat aacgcgttc 
      gttaaggcg attgaggag gcggcagca gcgcacgag 
      ttgaagaaa caatttaag gcgaggaaa tttgaagag 
      aagaatagt ggaatgaaa ccgccaccg tacgttcgg 
      attaaggtg aataaaccc gtggggaat gtgagggtg 
      tttgatggg aatacgagt aatacgacc tcttgtgat 
      tgtgatccg aaccagccg catccgtgt ggacctgat 
      agcgactgt ctaaacagg ctgcttttg accgaatgc 
      aaccccgac gtgtgcccg gccggcgac cgttgcaac 
      aaccaatgt tttgaaaaa cgcgaatac ccccctttg 
      gtgccccac cgcacactt taccgcggt tggggcctc 
      aaaaccctt gctccaatc cgaaaaggc caattcgta 
      atcgaatac gttggcgaa atgatcgac gaacaggag 
      taccaacgt cgcgtccaa aaaatgcac gaacaaaaa 
      gaagaaaac tactatttc ctcacgatc gacaaggac 
      aggatgttg gatgccggt ccgaaaggc aacgttgcc 
      agatttatg aaccattcg tgtgacccg aactgtgag 
      acacaaaaa tggacagtg aatggcgac accagagtt 
      ggattattc gcaaattgc gacattcca gccggcact 
      gagctcacc ttcaattac aatttagaa tgcattggg 
      aaagaaaag aagatctgt cactgtggc gcccccaat 
      tgtagtggc tttattggt gttaaagtg aaaacggat 
      aatcccccg aaaaagtcg actaaagcc aagaagaag 
      aaaacacca ccgcttgaa tatgtcgat aatgacaat 
      ccctgcttt atttgcggc aagcaaggc gacgttgcc 
      gcgtgcaat aataagatt tgtacaaag tattatcat 
      ttagcctgt gtggggctt gaggcatgg ccggaaggg 
      aacaagtgg gtgtgtcag tggcacaat tgcaacatt 
      tgcaccaag cgcacaatt cgctgttgc gtgcgatgc 
      attaactcg tattgtccg tctcactct gacggcaac 
      gtccgttac gataagctg ctagggttc gtctgtagt 
      gagcatgac tcgtcgaag gagtccaac gcggtcgtg 
      acgagccac gtgcccgca cccgattcg tgcgtcagt 
      gatgagggc gtggtcacg tcgacgact tccgatgat 
      tcacgaatg aacggattc gtcacctcc acgccggtg 
      aagaaaaac gacaattcg tacgatgag agtgtggtg 
      agttcgtcc gaaaagaaa ccgaagaag gttaaaaag 
      aagaaagtt gaaaacact gccaatttt ttactcgaa 
      aacgaagtc aagctgaat aatttcgcg cccgtgaag 
      tatttgagg actagtagg agtaggaat agtgaccct 
      gaaagggac ctcccgatt cgaaaacgt accagactt 
      agatgtgga agttcgttg tagaaattt gggttttaa 
      ttatttgta aacttt
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