| General information | |||
| TC012700 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 13393625..13403229 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC012700 | |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
|
|---|---|---|
| GO ID | * {{ blurIdResult }} |
|
| Evidence | {{ blurEvidenceResult }} |
|
| Reference | * {{ blurReferenceResult }} |
|
| Quotation | ||
| Name | ||
| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05299 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
head appendages random not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head appendages position irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
head shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC012700 RA
MASPQACLS SSPEIVSPG GKSRYGRTR RPKISHDFC NIDDVIIDD NPPEVKSPL KARDVVFEI KDEFTKLDD LGMKVANNF KTNRGLDAS IKERKFFKS AVVSKAVDV VDVSKVLKL VSTPGAEAQ AKSPPKTVL KTYSNRRKH AENERVVES FGLPDEPII PVKGHAFQR HSKIDVDFA AENDVSLPN PWTFDEIFT NEMKGPTNG ETFDENTDF QQTLTPKGA KNAFERTTG KKRLFRSSA EEGGTIVTV SPVLKKAKV GSSPPKRVL RSNFESNVK IQSSVSPQN ETNDSVVEA ELPSAGNES EEELPNISH KSLRSSTLK NKSPEKRIE ISHEKNKIK TKSPEKCKP KKGLNLVKT AKEISTETN KIRVGGTEC TVKNKSPKK SKTKEALNP VVTVEEISP EKNNVQDDA AEENPLKNG SSGKSKTKE ALNPVKMVK TSAEKNDGL EAVTKSALT MTQTKTKEV FNPVVIVTE ISPEKNNVQ EDVAENRTE ESGPKNKPK EVFLNPVII MKEFSAVKN DGPEAAESE PSMTQSKTK EVLEISAET NQVPENETK EELKVIKQE VIELEVGVL AWAKLGNFP YWPCLITQE PASGTHQKY VVNRIHPLY HVRFFGDKG RRSWVHGPN VMPFYAKDD LERLAKTLE VEGKFKPSY INECCFIRR TLMKKWQEG VAEAESLQF KTVDERLNY FSKMFARKN ELKQERQKL KRARLSAAE NLSKKVKIE EIETLELIK PEPVEVTSS PDTLIKPPK KKNFLKKEE FSDEQLEAR KKLRNPQVR EEIPQPSSA PKLRKKPQG ENSNSDTDS KFSDNSSLP PSMQSQAAL FRRNNIFRG IPREKVCQI CEKSGEIFK CKGPCNGVY HPECAMNRV VEQKSSVEI ISLESKQPL PDLGTSPNY TGMSLAEKI DHRMKEIMK KFDQSVYCE STDSSSDEY VVEEVVRQA SPPAQSKLS LTVTATPTK KEVPIIKHV TADNISYIC VSNEAPKED TTGLKCTWC ASDEAPLCL VCGEMGAQG RQKCSLHQC GRFYHPECL KLWPQTQWS LNASDSFVC PRHVCHTCI SDDPRAANS RCSSDKIVK CLKCPATYH SSNYCVPAG TEILTASQI ICPRHFTRN KRNYQSTIN ANWCFICSN GGDLICCET CPTSVHREC LPGDLGEVE TFFCEDCQS GRLPLYDEI VWVKLGSFR WWPAVILFP NEVPDNVKN IPHSKGEFV VKFYGTYDH YWVGRGRTF LFQEGDRGH SGSVKKRVD NAFVKAIEE AAAAHELKK QFKARKFEE KNSGMKPPP YVRIKVNKP VGNVRVFDG NTSNTTSCD CDPNQPHPC GPDSDCLNR LLLTECNPD VCPAGDRCN NQCFEKREY PPLVPHRTL YRGWGLKTL APIRKGQFV IEYVGEMID EQEYQRRVQ KMHEQKEEN YYFLTIDKD RMLDAGPKG NVARFMNHS CDPNCETQK WTVNGDTRV GLFANCDIP AGTELTFNY NLECIGKEK KICHCGAPN CSGFIGVKV KTDNPPKKS TKAKKKKTP PLEYVDNDN PCFICGKQG DVAACNNKI CTKYYHLAC VGLEAWPEG NKWVCQWHN CNICTKRTI RCCVRCINS YCPSHSDGN VRYDKLLGF VCSEHDSSK ESNAVVTSH VPAPDSCVS DEGVVTSTT SDDSRMNGF VTSTPVKKN DNSYDESVV SSSEKKPKK VKKKKVENT ANFLLENEV KLNNFAPVK YLRTSRSRN SDPERDLPI RKRTRLRCG SSL
- >TC012700 RA
atggcttcg cctcaagcc tgcctgagt tctagtccc gaaatagtc agccccgga ggcaagtcc cgctatggg cggaccagg cgccccaaa atctcgcac gacttttgc aacatcgac gacgttatc atcgacgat aaccccccg gaagtgaag tcgcccctc aaggcccgc gatgtcgtg ttcgagatc aaggacgag tttacgaaa ctcgacgac ttggggatg aaagtggcg aacaacttc aaaacgaac cgaggcttg gatgcttcg atcaaagag cgcaagttt ttcaaaagt gcggttgta agcaaggcg gtggatgtt gttgacgtg agcaaggtg cttaagttg gtttcgacg cccggtgcc gaggcccag gccaaaagc ccgccgaaa accgtcctg aagacgtac tcaaatcgg cggaaacac gccgagaac gagcgcgtt gttgagagt tttggtttg ccggacgaa ccgatcatt ccagtgaaa ggccacgct ttccagcga catagcaaa attgatgtc gattttgcg gccgaaaac gacgtgagt ttgccgaat ccgtggacg tttgatgag atttttacc aacgagatg aagggaccc acgaatgga gaaaccttt gatgaaaac accgatttt caacaaacg ctcaccccc aagggggcc aaaaacgcc ttcgaacgt actacagga aagaagagg ttgttccgc agttctgca gaggaaggg ggcacaata gttacagtt tcaccagtg ttaaagaaa gccaaagtt gggagtagc cccccgaag agggttctc aggtcgaat tttgagtca aatgtgaaa atccagtcg tctgtttca ccacaaaat gaaacaaat gatagtgta gtggaggca gagttgcca agtgcgggc aatgaatca gaagaggag ttgccaaat ataagtcac aagtcacta agatcaagc actctaaaa aacaagtca cccgagaaa agaatagaa atttcccac gaaaaaaat aaaataaaa accaagtca cctgaaaaa tgtaagcca aaaaaaggc ttaaatctg gtaaaaaca gcaaaagaa atttccacc gaaacaaat aaaatacgg gttggtggg acggagtgt actgtaaaa aacaagtcg cctaaaaaa agtaaaaca aaagaagcc ttaaatccc gtagtaaca gtcgaagag atctctccc gaaaaaaat aacgtacag gacgatgcg gcggaagaa aatcctcta aaaaatggc tcgtctgga aaaagtaaa acaaaggaa gccttaaat ccggtaaaa atggtgaaa acttccgcc gaaaaaaac gacggactt gaagccgtg acaaaaagc gcgctgaca atgacacaa actaaaaca aaagaagtc ttcaatcct gtagtaata gtgacagag atttctccc gaaaagaat aacgtacag gaagatgtg gcagaaaac aggacggag gaaagtggt ccaaaaaat aaaccaaaa gaagttttt ttaaatcct gtaataatc atgaaagaa ttttccgct gtaaaaaac gacggccct gaggctgca gaaagcgag ccgtcaatg acacaaagt aaaacaaaa gaagtctta gaaatttcc gccgaaaca aatcaagta cccgaaaat gagacgaaa gaggaattg aaagttatt aaacaagaa gtaatagag ctcgaagtt ggggttctg gcctgggct aaacttggc aattttccc tactggccg tgtctgatt acgcaagaa ccggcgagt ggaacgcat caaaaatac gttgtgaat cggatccat ccgctttat cacgtgaga tttttcggt gataaaggc cgaaggtca tgggtgcat ggcccaaac gtgatgcct ttttacgcc aaagacgat ctcgaacgt cttgcaaaa acgctcgaa gtcgaggga aaatttaaa ccatcctat atcaatgaa tgttgcttt ataagacgc accctaatg aagaaatgg caagaagga gtcgctgaa gccgaatct ttacaattc aaaacagtc gacgaacgc ttgaattac ttttcgaag atgtttgcc agaaaaaac gaactgaag caagaaaga caaaaactg aaacgtgcg cggttatca gccgccgaa aacttgtcg aaaaaagtg aaaatcgaa gaaattgaa acacttgaa ttgatcaaa ccggaacca gtcgaagtt acttcgagc cccgacact ttaataaaa ccccccaaa aagaagaat ttcttaaag aaggaagaa ttttccgac gaacaactc gaagcgcgc aagaaacta cgcaatcca caagtgcgg gaagaaatc ccccaacct tcgagcgcc ccgaagttg cgtaaaaag ccccaaggt gaaaattca aactcggac accgactcc aaattcagc gacaacagc tcgctccca ccctccatg caatcgcag gcggccctg tttcgccgc aacaacatc ttccgggga atccctcgc gagaaagtg tgccaaata tgcgaaaaa tccggggaa atcttcaaa tgcaagggc ccctgtaac ggcgtttac caccccgag tgtgccatg aatcgcgtc gtggaacaa aaaagcagc gtcgaaata atctcactc gaatcgaag caacccttg cccgacctg ggtacttcc cccaactac acgggaatg tccctagct gagaaaatc gaccatcgg atgaaagaa ataatgaaa aagtttgac caatcggtt tattgcgaa tcgaccgat tccagtagc gatgagtac gttgtagaa gaggtggtt agacaagcc tcgccccca gcccaatcg aaactctcc ctcaccgtc accgccacg cccacgaag aaggaagta ccaatcata aagcacgtc actgccgac aatatcagt tacatttgc gtgagtaat gaggcacca aaggaggac accacgggt ttgaaatgc acgtggtgt gcctcagac gaggccccc ttgtgcctc gtgtgtggg gagatgggg gcccaaggg cgccagaag tgctcgctg caccaatgc ggccgtttc taccacccc gagtgcctc aaactgtgg ccgcagacg cagtggtcg ctgaacgcg tcggacagc ttcgtgtgt cctcggcac gtgtgccac acgtgcatt tccgacgat ccccgagcg gcgaactcg cgctgttcg agcgataaa atcgtaaaa tgcctcaaa tgccccgcc acctaccac tcgagcaat tactgcgtg cctgcgggc acggaaatt ctgaccgcc tcccagatt atatgcccg aggcatttt accagaaac aagcgcaac taccaatca acgatcaac gccaattgg tgtttcatt tgttcgaac gggggcgac ttgatctgc tgcgagacg tgtccgacc agtgtgcac agggagtgt cttccgggt gatttgggc gaggttgag acgtttttc tgtgaggac tgccaatcg gggcggttg cccctctat gatgagatc gtttgggtg aagctcggg tcgtttcgg tggtggccg gccgtgatc ttgttcccg aatgaggtt ccagataac gtgaaaaat atcccgcat agtaagggg gagtttgtg gtcaagttt tacgggacg tacgaccat tattgggtc gggagaggg aggacgttc ttgtttcag gaaggcgat cggggacat tcgggttcg gtgaagaag cgagtcgat aacgcgttc gttaaggcg attgaggag gcggcagca gcgcacgag ttgaagaaa caatttaag gcgaggaaa tttgaagag aagaatagt ggaatgaaa ccgccaccg tacgttcgg attaaggtg aataaaccc gtggggaat gtgagggtg tttgatggg aatacgagt aatacgacc tcttgtgat tgtgatccg aaccagccg catccgtgt ggacctgat agcgactgt ctaaacagg ctgcttttg accgaatgc aaccccgac gtgtgcccg gccggcgac cgttgcaac aaccaatgt tttgaaaaa cgcgaatac ccccctttg gtgccccac cgcacactt taccgcggt tggggcctc aaaaccctt gctccaatc cgaaaaggc caattcgta atcgaatac gttggcgaa atgatcgac gaacaggag taccaacgt cgcgtccaa aaaatgcac gaacaaaaa gaagaaaac tactatttc ctcacgatc gacaaggac aggatgttg gatgccggt ccgaaaggc aacgttgcc agatttatg aaccattcg tgtgacccg aactgtgag acacaaaaa tggacagtg aatggcgac accagagtt ggattattc gcaaattgc gacattcca gccggcact gagctcacc ttcaattac aatttagaa tgcattggg aaagaaaag aagatctgt cactgtggc gcccccaat tgtagtggc tttattggt gttaaagtg aaaacggat aatcccccg aaaaagtcg actaaagcc aagaagaag aaaacacca ccgcttgaa tatgtcgat aatgacaat ccctgcttt atttgcggc aagcaaggc gacgttgcc gcgtgcaat aataagatt tgtacaaag tattatcat ttagcctgt gtggggctt gaggcatgg ccggaaggg aacaagtgg gtgtgtcag tggcacaat tgcaacatt tgcaccaag cgcacaatt cgctgttgc gtgcgatgc attaactcg tattgtccg tctcactct gacggcaac gtccgttac gataagctg ctagggttc gtctgtagt gagcatgac tcgtcgaag gagtccaac gcggtcgtg acgagccac gtgcccgca cccgattcg tgcgtcagt gatgagggc gtggtcacg tcgacgact tccgatgat tcacgaatg aacggattc gtcacctcc acgccggtg aagaaaaac gacaattcg tacgatgag agtgtggtg agttcgtcc gaaaagaaa ccgaagaag gttaaaaag aagaaagtt gaaaacact gccaatttt ttactcgaa aacgaagtc aagctgaat aatttcgcg cccgtgaag tatttgagg actagtagg agtaggaat agtgaccct gaaagggac ctcccgatt cgaaaacgt accagactt agatgtgga agttcgttg tag
- >TC012700 RA
ccatccaaa aaatgaaaa agcgggaaa gtgcaaaaa agaatcggc gccaacttc acgtcacaa catccaaaa acttgcact aaaaaccga tccaaaaca aaaatattg ataagttcc tattccact gtcgcccca aaatggttc aaaattatt atcagggtc cacgtccag gcagttaaa tggctgttg gcttgtgtg atggcttcg cctcaagcc tgcctgagt tctagtccc gaaatagtc agccccgga ggcaagtcc cgctatggg cggaccagg cgccccaaa atctcgcac gacttttgc aacatcgac gacgttatc atcgacgat aaccccccg gaagtgaag tcgcccctc aaggcccgc gatgtcgtg ttcgagatc aaggacgag tttacgaaa ctcgacgac ttggggatg aaagtggcg aacaacttc aaaacgaac cgaggcttg gatgcttcg atcaaagag cgcaagttt ttcaaaagt gcggttgta agcaaggcg gtggatgtt gttgacgtg agcaaggtg cttaagttg gtttcgacg cccggtgcc gaggcccag gccaaaagc ccgccgaaa accgtcctg aagacgtac tcaaatcgg cggaaacac gccgagaac gagcgcgtt gttgagagt tttggtttg ccggacgaa ccgatcatt ccagtgaaa ggccacgct ttccagcga catagcaaa attgatgtc gattttgcg gccgaaaac gacgtgagt ttgccgaat ccgtggacg tttgatgag atttttacc aacgagatg aagggaccc acgaatgga gaaaccttt gatgaaaac accgatttt caacaaacg ctcaccccc aagggggcc aaaaacgcc ttcgaacgt actacagga aagaagagg ttgttccgc agttctgca gaggaaggg ggcacaata gttacagtt tcaccagtg ttaaagaaa gccaaagtt gggagtagc cccccgaag agggttctc aggtcgaat tttgagtca aatgtgaaa atccagtcg tctgtttca ccacaaaat gaaacaaat gatagtgta gtggaggca gagttgcca agtgcgggc aatgaatca gaagaggag ttgccaaat ataagtcac aagtcacta agatcaagc actctaaaa aacaagtca cccgagaaa agaatagaa atttcccac gaaaaaaat aaaataaaa accaagtca cctgaaaaa tgtaagcca aaaaaaggc ttaaatctg gtaaaaaca gcaaaagaa atttccacc gaaacaaat aaaatacgg gttggtggg acggagtgt actgtaaaa aacaagtcg cctaaaaaa agtaaaaca aaagaagcc ttaaatccc gtagtaaca gtcgaagag atctctccc gaaaaaaat aacgtacag gacgatgcg gcggaagaa aatcctcta aaaaatggc tcgtctgga aaaagtaaa acaaaggaa gccttaaat ccggtaaaa atggtgaaa acttccgcc gaaaaaaac gacggactt gaagccgtg acaaaaagc gcgctgaca atgacacaa actaaaaca aaagaagtc ttcaatcct gtagtaata gtgacagag atttctccc gaaaagaat aacgtacag gaagatgtg gcagaaaac aggacggag gaaagtggt ccaaaaaat aaaccaaaa gaagttttt ttaaatcct gtaataatc atgaaagaa ttttccgct gtaaaaaac gacggccct gaggctgca gaaagcgag ccgtcaatg acacaaagt aaaacaaaa gaagtctta gaaatttcc gccgaaaca aatcaagta cccgaaaat gagacgaaa gaggaattg aaagttatt aaacaagaa gtaatagag ctcgaagtt ggggttctg gcctgggct aaacttggc aattttccc tactggccg tgtctgatt acgcaagaa ccggcgagt ggaacgcat caaaaatac gttgtgaat cggatccat ccgctttat cacgtgaga tttttcggt gataaaggc cgaaggtca tgggtgcat ggcccaaac gtgatgcct ttttacgcc aaagacgat ctcgaacgt cttgcaaaa acgctcgaa gtcgaggga aaatttaaa ccatcctat atcaatgaa tgttgcttt ataagacgc accctaatg aagaaatgg caagaagga gtcgctgaa gccgaatct ttacaattc aaaacagtc gacgaacgc ttgaattac ttttcgaag atgtttgcc agaaaaaac gaactgaag caagaaaga caaaaactg aaacgtgcg cggttatca gccgccgaa aacttgtcg aaaaaagtg aaaatcgaa gaaattgaa acacttgaa ttgatcaaa ccggaacca gtcgaagtt acttcgagc cccgacact ttaataaaa ccccccaaa aagaagaat ttcttaaag aaggaagaa ttttccgac gaacaactc gaagcgcgc aagaaacta cgcaatcca caagtgcgg gaagaaatc ccccaacct tcgagcgcc ccgaagttg cgtaaaaag ccccaaggt gaaaattca aactcggac accgactcc aaattcagc gacaacagc tcgctccca ccctccatg caatcgcag gcggccctg tttcgccgc aacaacatc ttccgggga atccctcgc gagaaagtg tgccaaata tgcgaaaaa tccggggaa atcttcaaa tgcaagggc ccctgtaac ggcgtttac caccccgag tgtgccatg aatcgcgtc gtggaacaa aaaagcagc gtcgaaata atctcactc gaatcgaag caacccttg cccgacctg ggtacttcc cccaactac acgggaatg tccctagct gagaaaatc gaccatcgg atgaaagaa ataatgaaa aagtttgac caatcggtt tattgcgaa tcgaccgat tccagtagc gatgagtac gttgtagaa gaggtggtt agacaagcc tcgccccca gcccaatcg aaactctcc ctcaccgtc accgccacg cccacgaag aaggaagta ccaatcata aagcacgtc actgccgac aatatcagt tacatttgc gtgagtaat gaggcacca aaggaggac accacgggt ttgaaatgc acgtggtgt gcctcagac gaggccccc ttgtgcctc gtgtgtggg gagatgggg gcccaaggg cgccagaag tgctcgctg caccaatgc ggccgtttc taccacccc gagtgcctc aaactgtgg ccgcagacg cagtggtcg ctgaacgcg tcggacagc ttcgtgtgt cctcggcac gtgtgccac acgtgcatt tccgacgat ccccgagcg gcgaactcg cgctgttcg agcgataaa atcgtaaaa tgcctcaaa tgccccgcc acctaccac tcgagcaat tactgcgtg cctgcgggc acggaaatt ctgaccgcc tcccagatt atatgcccg aggcatttt accagaaac aagcgcaac taccaatca acgatcaac gccaattgg tgtttcatt tgttcgaac gggggcgac ttgatctgc tgcgagacg tgtccgacc agtgtgcac agggagtgt cttccgggt gatttgggc gaggttgag acgtttttc tgtgaggac tgccaatcg gggcggttg cccctctat gatgagatc gtttgggtg aagctcggg tcgtttcgg tggtggccg gccgtgatc ttgttcccg aatgaggtt ccagataac gtgaaaaat atcccgcat agtaagggg gagtttgtg gtcaagttt tacgggacg tacgaccat tattgggtc gggagaggg aggacgttc ttgtttcag gaaggcgat cggggacat tcgggttcg gtgaagaag cgagtcgat aacgcgttc gttaaggcg attgaggag gcggcagca gcgcacgag ttgaagaaa caatttaag gcgaggaaa tttgaagag aagaatagt ggaatgaaa ccgccaccg tacgttcgg attaaggtg aataaaccc gtggggaat gtgagggtg tttgatggg aatacgagt aatacgacc tcttgtgat tgtgatccg aaccagccg catccgtgt ggacctgat agcgactgt ctaaacagg ctgcttttg accgaatgc aaccccgac gtgtgcccg gccggcgac cgttgcaac aaccaatgt tttgaaaaa cgcgaatac ccccctttg gtgccccac cgcacactt taccgcggt tggggcctc aaaaccctt gctccaatc cgaaaaggc caattcgta atcgaatac gttggcgaa atgatcgac gaacaggag taccaacgt cgcgtccaa aaaatgcac gaacaaaaa gaagaaaac tactatttc ctcacgatc gacaaggac aggatgttg gatgccggt ccgaaaggc aacgttgcc agatttatg aaccattcg tgtgacccg aactgtgag acacaaaaa tggacagtg aatggcgac accagagtt ggattattc gcaaattgc gacattcca gccggcact gagctcacc ttcaattac aatttagaa tgcattggg aaagaaaag aagatctgt cactgtggc gcccccaat tgtagtggc tttattggt gttaaagtg aaaacggat aatcccccg aaaaagtcg actaaagcc aagaagaag aaaacacca ccgcttgaa tatgtcgat aatgacaat ccctgcttt atttgcggc aagcaaggc gacgttgcc gcgtgcaat aataagatt tgtacaaag tattatcat ttagcctgt gtggggctt gaggcatgg ccggaaggg aacaagtgg gtgtgtcag tggcacaat tgcaacatt tgcaccaag cgcacaatt cgctgttgc gtgcgatgc attaactcg tattgtccg tctcactct gacggcaac gtccgttac gataagctg ctagggttc gtctgtagt gagcatgac tcgtcgaag gagtccaac gcggtcgtg acgagccac gtgcccgca cccgattcg tgcgtcagt gatgagggc gtggtcacg tcgacgact tccgatgat tcacgaatg aacggattc gtcacctcc acgccggtg aagaaaaac gacaattcg tacgatgag agtgtggtg agttcgtcc gaaaagaaa ccgaagaag gttaaaaag aagaaagtt gaaaacact gccaatttt ttactcgaa aacgaagtc aagctgaat aatttcgcg cccgtgaag tatttgagg actagtagg agtaggaat agtgaccct gaaagggac ctcccgatt cgaaaacgt accagactt agatgtgga agttcgttg tagaaattt gggttttaa ttatttgta aacttt
