Status
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Gene information
General information
TC012698 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 13339175..13342469
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012698
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05298

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012698 RA
      MSRLLKLAQ KFNCFYLSG LSSGDCRPF VVEGFQVGL 
      VRPDVMSQL LKFPEVFRI TSGCVELNP AFRDYEERS 
      SQVDRVLRE LRAGSVFIA LKGWRDECY EVKTDFTSK 
      SLLKMDRSA TCLFGIRNY GVDINGYVR HPKLGLCLW 
      LQKRAATKQ TWPGKWDNM VGGGLSVGH GIFETAYKE 
      AMEEASIPA ELMKNLVSA GCVSFFFES ERGLFPNTE 
      FVFDLELPL DFIPENADG EVETFELLP AEQCLEKLF 
      SSDFKTTSV PVALDFLIR HGMITSENE KEFIKIVEL 
      LHVPLQSIY NHTQTVSNS IKENGTTRV NNV
    • >TC012698 RB
      MSVQQSNKN MSRLLKLAQ KFNCFYLSG LSSGDCRPF 
      VVEGFQVGL VRPDVMSQL LKFPEVFRI TSGCVELNP 
      AFRDYEERS SQVDRVLRE LRAGSVFIA LKGWRDECY 
      EVKTDFTSK SLLKMDRSA TCLFGIRNY GVDINGYVR 
      HPKLGLCLW LQKRAATKQ TWPGKWDNM VGGGLSVGH 
      GIFETAYKE AMEEASIPA ELMKNLVSA GCVSFFFES 
      ERGLFPNTE FVFDLELPL DFIPENADG EVETFELLP 
      AEQCLEKLF SSDFKTTSV PVALDFLIR HGMITSENE 
      KEFIKIVEL LHVPLQSIY NHTQTVSNS IKENGTTRV 
      NNV
    CDS sequence
    • >TC012698 RA
      atgtcaaga ttacttaaa ctcgcccaa aaatttaat 
      tgtttctac ttgtcaggt ctgtcgtcg ggcgactgc 
      cggcccttc gtggtggag ggcttccag gtggggctc 
      gtccggccg gacgtcatg agccagctc ctcaagttc 
      cccgaagtt ttccgcata acgtccggc tgcgtggag 
      ctcaacccc gcgttccgc gactacgag gagcgcagc 
      tcgcaggtg gaccgcgtc ttgcgcgaa ctgcgcgcc 
      ggcagcgtc ttcatcgcg ctgaagggc tggcgcgac 
      gagtgctac gaggtcaag actgacttc acctcgaag 
      agtctgctc aagatggac cgctcggcg acgtgtctc 
      ttcggcata cgcaactac ggggtggac atcaacggg 
      tacgtgcgg caccccaag ctggggctg tgtctgtgg 
      ctgcagaag cgcgccgcc accaagcag acctggcca 
      ggtaaatgg gacaacatg gtgggcggg gggctgtcg 
      gtggggcac ggcattttc gagacggcc tacaaggag 
      gccatggag gaggcgtcc atcccagcc gagttgatg 
      aagaacttg gtcagcgcg ggatgtgtc tcgtttttt 
      ttcgaatct gaaaggggc ttattcccc aatacagag 
      tttgttttc gatttggaa cttccatta gattttatt 
      ccagaaaat gcggatggg gaagttgaa acttttgaa 
      ttgcttcca gctgaacag tgtctagag aaattgttt 
      agttctgat tttaagacg acgagtgtt ccagtagcg 
      ttagatttt ttgattagg cacgggatg attacgtca 
      gagaatgaa aaggagttt atcaaaatt gtggaactg 
      ctccacgtg ccgctccag tctatttac aatcacact 
      caaacggtt agcaactca atcaaggaa aacgggacg 
      acgagagtg aacaacgta taa
    • >TC012698 RB
      atgtcagtg caacagtcc aacaagaac atgtcaaga 
      ttacttaaa ctcgcccaa aaatttaat tgtttctac 
      ttgtcaggt ctgtcgtcg ggcgactgc cggcccttc 
      gtggtggag ggcttccag gtggggctc gtccggccg 
      gacgtcatg agccagctc ctcaagttc cccgaagtt 
      ttccgcata acgtccggc tgcgtggag ctcaacccc 
      gcgttccgc gactacgag gagcgcagc tcgcaggtg 
      gaccgcgtc ttgcgcgaa ctgcgcgcc ggcagcgtc 
      ttcatcgcg ctgaagggc tggcgcgac gagtgctac 
      gaggtcaag actgacttc acctcgaag agtctgctc 
      aagatggac cgctcggcg acgtgtctc ttcggcata 
      cgcaactac ggggtggac atcaacggg tacgtgcgg 
      caccccaag ctggggctg tgtctgtgg ctgcagaag 
      cgcgccgcc accaagcag acctggcca ggtaaatgg 
      gacaacatg gtgggcggg gggctgtcg gtggggcac 
      ggcattttc gagacggcc tacaaggag gccatggag 
      gaggcgtcc atcccagcc gagttgatg aagaacttg 
      gtcagcgcg ggatgtgtc tcgtttttt ttcgaatct 
      gaaaggggc ttattcccc aatacagag tttgttttc 
      gatttggaa cttccatta gattttatt ccagaaaat 
      gcggatggg gaagttgaa acttttgaa ttgcttcca 
      gctgaacag tgtctagag aaattgttt agttctgat 
      tttaagacg acgagtgtt ccagtagcg ttagatttt 
      ttgattagg cacgggatg attacgtca gagaatgaa 
      aaggagttt atcaaaatt gtggaactg ctccacgtg 
      ccgctccag tctatttac aatcacact caaacggtt 
      agcaactca atcaaggaa aacgggacg acgagagtg 
      aacaacgta taa
    mRNA sequence
    • >TC012698 RA
      gttccaatg tcagtgcaa cagtccaac aagaacatg 
      tcaagatta cttaaactc gcccaaaaa tttaattgt 
      ttctacttg tcaggtctg tcgtcgggc gactgccgg 
      cccttcgtg gtggagggc ttccaggtg gggctcgtc 
      cggccggac gtcatgagc cagctcctc aagttcccc 
      gaagttttc cgcataacg tccggctgc gtggagctc 
      aaccccgcg ttccgcgac tacgaggag cgcagctcg 
      caggtggac cgcgtcttg cgcgaactg cgcgccggc 
      agcgtcttc atcgcgctg aagggctgg cgcgacgag 
      tgctacgag gtcaagact gacttcacc tcgaagagt 
      ctgctcaag atggaccgc tcggcgacg tgtctcttc 
      ggcatacgc aactacggg gtggacatc aacgggtac 
      gtgcggcac cccaagctg gggctgtgt ctgtggctg 
      cagaagcgc gccgccacc aagcagacc tggccaggt 
      aaatgggac aacatggtg ggcgggggg ctgtcggtg 
      gggcacggc attttcgag acggcctac aaggaggcc 
      atggaggag gcgtccatc ccagccgag ttgatgaag 
      aacttggtc agcgcggga tgtgtctcg ttttttttc 
      gaatctgaa aggggctta ttccccaat acagagttt 
      gttttcgat ttggaactt ccattagat tttattcca 
      gaaaatgcg gatggggaa gttgaaact tttgaattg 
      cttccagct gaacagtgt ctagagaaa ttgtttagt 
      tctgatttt aagacgacg agtgttcca gtagcgtta 
      gattttttg attaggcac gggatgatt acgtcagag 
      aatgaaaag gagtttatc aaaattgtg gaactgctc 
      cacgtgccg ctccagtct atttacaat cacactcaa 
      acggttagc aactcaatc aaggaaaac gggacgacg 
      agagtgaac aacgtataa ttgccgctt taggtttcc 
      gatcttagg ttttttcta gtagaatag aaaagaagt 
      gagccttaa ataatttcc taatttatt ttatacaaa 
      gtcgcaatt tttcacaac aaatagtag agagctttt 
      gtaattatt gtatatttt gtctattaa tttatttac 
      aattttata caatgtagg aatttagac gtaatactg 
      aaatcaatt tatttatta ctatacata ctgcgaaat 
      catcacagt tctcatatt gtggggttt tacgtacta 
      cttgtgata atacaatta attctcgtt ttttgaatg 
      tacttacgt gtactcaac gaaatgtat ttattatac 
      aacatatat acaataaaa actcaaagg cgtcaagct 
      gtttgaatt tcccgccac cgtcaaaca atcggctaa 
      tgtccacta ctcactcgt gaagtcaat gattatcgc 
      aaaattttc aaaacctgt aatatttta ccaggcttt 
      acagccaaa aacgcggtt tattttcaa aatttcctt 
      aaaaacaag ctaaaaact acgaaatcg gcccaaaaa 
      attgtgttt gatattttt gtgtttcga aagcacatt 
      tttgagtta atttatgtt atagataaa cccaaaaaa 
      gaatgaaat ttagcactt cttaagtgc taaacgtga
    • >TC012698 RB
      gttccaatg tcagtgcaa cagtccaac aagaacatg 
      tcaagatta cttaaactc gcccaaaaa tttaattgt 
      ttctacttg tcaggtctg tcgtcgggc gactgccgg 
      cccttcgtg gtggagggc ttccaggtg gggctcgtc 
      cggccggac gtcatgagc cagctcctc aagttcccc 
      gaagttttc cgcataacg tccggctgc gtggagctc 
      aaccccgcg ttccgcgac tacgaggag cgcagctcg 
      caggtggac cgcgtcttg cgcgaactg cgcgccggc 
      agcgtcttc atcgcgctg aagggctgg cgcgacgag 
      tgctacgag gtcaagact gacttcacc tcgaagagt 
      ctgctcaag atggaccgc tcggcgacg tgtctcttc 
      ggcatacgc aactacggg gtggacatc aacgggtac 
      gtgcggcac cccaagctg gggctgtgt ctgtggctg 
      cagaagcgc gccgccacc aagcagacc tggccaggt 
      aaatgggac aacatggtg ggcgggggg ctgtcggtg 
      gggcacggc attttcgag acggcctac aaggaggcc 
      atggaggag gcgtccatc ccagccgag ttgatgaag 
      aacttggtc agcgcggga tgtgtctcg ttttttttc 
      gaatctgaa aggggctta ttccccaat acagagttt 
      gttttcgat ttggaactt ccattagat tttattcca 
      gaaaatgcg gatggggaa gttgaaact tttgaattg 
      cttccagct gaacagtgt ctagagaaa ttgtttagt 
      tctgatttt aagacgacg agtgttcca gtagcgtta 
      gattttttg attaggcac gggatgatt acgtcagag 
      aatgaaaag gagtttatc aaaattgtg gaactgctc 
      cacgtgccg ctccagtct atttacaat cacactcaa 
      acggttagc aactcaatc aaggaaaac gggacgacg 
      agagtgaac aacgtataa ttgccgctt taggtttcc 
      gatcttagg ttttttcta gtagaatag aaaagaagt 
      gagccttaa ataatttcc taatttatt ttatacaaa 
      gtcgcaatt tttcacaac aaatagtag agagctttt 
      gtaattatt gtatatttt gtctattaa tttatttac 
      aattttata caatgtagg aatttagac gtaatactg 
      aaatcaatt tatttatta ctatacata ctgcgaaat 
      catcacagt tctcatatt gtggggttt tacgtacta 
      cttgtgata atacaatta attctcgtt ttttgaatg 
      tacttacgt gtactcaac gaaatgtat ttattatac 
      aacatatat acaataaaa actcaaagg cgtcaagct 
      gtttgaatt tcccgccac cgtcaaaca atcggctaa 
      tgtccacta ctcactcgt gaagtcaat gattatcgc 
      aaaattttc aaaacctgt aatatttta ccaggcttt 
      acagccaaa aacgcggtt tattttcaa aatttcctt 
      aaaaacaag ctaaaaact acgaaatcg gcccaaaaa 
      attgtgttt gatattttt gtgtttcga aagcacatt 
      tttgagtta atttatgtt atagataaa cccaaaaaa 
      gaatgaaat ttagcactt cttaagtgc taaacgtga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY