Status
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Gene information
General information
TC031248
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC012695,TC012696
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 13323326..13325631
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC031248
      iBeetle screen iB_05296

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

      larva dorsal not closed - Penetrance (percentage of animals/eggs on slide): less than 30%

      head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

      thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC031248 RA
        MNRKILYSK TTDSYSEKV LIGEWFNRR HNVKPPLPQ 
        NHDFLTVYR ESFTPKPYS KWDTALMWD HLMKNEDSN 
        FISQNECYE STNFFDNYC STNDLAYNY FPKCVKKPI 
        ARTWRMKKG WHEPIDEFL PSFGNLTSF GLVKQKKSQ 
        WECEKNFGL FTSYEADFR APRPEDYKF DKFLFPKVN 
        CVRLKMRGK NSVSQSRLV FITPSTYSN KVLIGPWVY 
        DRLNAQYSP PEHKDHVTT YRDHFQPKQ NSGSDLTAV 
        WDQKLKSED SNFIPQTGR SDFPNYFDN FSSIYDLSF 
        NHFPKCLDG RLARKRQRN GHCIPLEEY LPTFGNMTQ 
        FGLVESKQE QWKCETQDP RRNKVTHYS DHYAAPELS 
        AYKFKRFAI PLANSSIMD ATNLTTMAL NLRNRPTFK 
        VVPPQDLVP VPRPPHLNP ITWECIPEK PKVSKKCI
      CDS sequence
      • >TC031248 RA
        atgaatcgg aaaattttg tactcgaaa accaccgat 
        tcgtactcc gaaaaagta ctgatcggc gaatggttc 
        aaccgtcgc cacaatgtg aaaccaccg ttgcctcaa 
        aaccacgat tttctcaca gtttatcgc gaatcgttc 
        actccaaaa ccctactca aaatgggac acggccctc 
        atgtgggac cacctcatg aaaaacgaa gacagtaat 
        tttatctct caaaacgag tgctacgag agtacaaac 
        tttttcgat aactactgt agtaccaac gatttggcc 
        tacaattat ttcccaaaa tgtgtcaaa aagccgatt 
        gccagaacg tggcggatg aaaaaaggg tggcacgag 
        cccatcgac gagtttctc cccagtttt ggcaatttg 
        accagcttc ggacttgta aaacagaaa aagagccag 
        tgggagtgt gagaagaac ttcggactg tttacaagc 
        tacgaagct gattttagg gcccctaga ccagaggat 
        tacaagttt gataaattc ttgtttccc aaagtcaac 
        tgtgttcga ttaaaaatg cgcggcaaa aactcagtg 
        tctcaatcc cgcctagtt ttcataact ccgtccact 
        tattcgaat aaagtgcta atcgggcct tgggtttac 
        gaccgtctt aacgcccag tacagcccc ccggagcac 
        aaagaccac gtgacaacc taccgtgac cactttcaa 
        ccgaaacag aattcggga agcgacctt acggcagtc 
        tgggaccaa aaactaaaa tctgaagac agtaatttc 
        atcccccaa accgggcgt agcgacttc cccaattac 
        tttgacaac ttttccagt atttacgac ttgtccttt 
        aatcatttt cccaaatgt ctggacggg cgtttggcg 
        cggaaacga caaaggaac ggtcattgt atcccactt 
        gaggagtat ttgcccact tttggcaat atgacgcag 
        tttggactc gtggagagc aagcaggaa caatggaag 
        tgcgaaacg caggacccg cgtcggaat aaagtcaca 
        cactacagt gaccattac gcggccccc gaactcagt 
        gcttacaaa tttaagcga ttcgcgatc cctctggcg 
        aattcgtcc ataatggac gctaccaac ctaacaacc 
        atggccctt aacttaaga aaccgacca acatttaag 
        gtggtaccc cctcaagac ttggtcccg gtccccaga 
        cccccccat ctgaacccc ataacgtgg gaatgcata 
        ccagaaaaa ccaaaagtt tcaaaaaaa tgtatttaa
      mRNA sequence
      • >TC031248 RA
        tccatttga caagacaat aatttaatg tcatgaatc 
        ggaaaattt tgtactcga aaaccaccg attcgtact 
        ccgaaaaag tactgatcg gcgaatggt tcaaccgtc 
        gccacaatg tgaaaccac cgttgcctc aaaaccacg 
        attttctca cagtttatc gcgaatcgt tcactccaa 
        aaccctact caaaatggg acacggccc tcatgtggg 
        accacctca tgaaaaacg aagacagta attttatct 
        ctcaaaacg agtgctacg agagtacaa actttttcg 
        ataactact gtagtacca acgatttgg cctacaatt 
        atttcccaa aatgtgtca aaaagccga ttgccagaa 
        cgtggcgga tgaaaaaag ggtggcacg agcccatcg 
        acgagtttc tccccagtt ttggcaatt tgaccagct 
        tcggacttg taaaacaga aaaagagcc agtgggagt 
        gtgagaaga acttcggac tgtttacaa gctacgaag 
        ctgatttta gggccccta gaccagagg attacaagt 
        ttgataaat tcttgtttc ccaaagtca actgtgttc 
        gattaaaaa tgcgcggca aaaactcag tgtctcaat 
        cccgcctag ttttcataa ctccgtcca cttattcga 
        ataaagtgc taatcgggc cttgggttt acgaccgtc 
        ttaacgccc agtacagcc ccccggagc acaaagacc 
        acgtgacaa cctaccgtg accactttc aaccgaaac 
        agaattcgg gaagcgacc ttacggcag tctgggacc 
        aaaaactaa aatctgaag acagtaatt tcatccccc 
        aaaccgggc gtagcgact tccccaatt actttgaca 
        acttttcca gtatttacg acttgtcct ttaatcatt 
        ttcccaaat gtctggacg ggcgtttgg cgcggaaac 
        gacaaagga acggtcatt gtatcccac ttgaggagt 
        atttgccca cttttggca atatgacgc agtttggac 
        tcgtggaga gcaagcagg aacaatgga agtgcgaaa 
        cgcaggacc cgcgtcgga ataaagtca cacactaca 
        gtgaccatt acgcggccc ccgaactca gtgcttaca 
        aatttaagc gattcgcga tccctctgg cgaattcgt 
        ccataatgg acgctacca acctaacaa ccatggccc 
        ttaacttaa gaaaccgac caacattta aggtggtac 
        cccctcaag acttggtcc cggtcccca gaccccccc 
        atctgaacc ccataacgt gggaatgca taccagaaa 
        aaccaaaag tttcaaaaa aatgtattt aaaacaaat 
        acagcttat ctcataca
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY