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Gene information
General information
TC012687 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 13170730..13172641
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012687
    iBeetle screen iB_05295

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Fertility
    tropharium structure irregular - Number of animals showing the phenotype: 4

    tropharium sometimes slightlyshortened - Number of animals showing the phenotype: 4

    vitellarium mostly not present - Number of animals showing the phenotype: 4
    egg chamber ectopically present - Number of animals showing the phenotype: 2

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    about 25

    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection



    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    Comment: below the larval cuticle pupal (gin traps) and adult (abdominal sternites) structures are visible

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012687 RA
      MSKPVKKPR KKATTGYKM PEPLPAGEV LVDVGKNQW 
      RLGPSIGKG GFGEIYAAQ EASSKSPKY PYVVKVEPH 
      GNGPLFVEM HFYMKNAKQ ADIEAFMKK HKLKTFGMP 
      AYLGSGSHE MGASKYRFI VMEKFGADI DQLRRDKGA 
      LSSTSVFQM GWQITYVLE YIHDFGYIH GDIKGGNVL 
      LGVNKSQVY LVDFGLATK FSTEREFKP NPKKAHDGT 
      IEYTSRDAH HGVPTRRGD FEILGFNLI HWLGGTLPW 
      DSHLSDPKT VQQLKEDNL KDVPKFIKT CLGKKSETL 
      IKYFQYVNS LKFDEVPDY KKIRSLFED EIRKNGKTV 
      DSPFDFLGT VKKRRSLCG DRLDDGDDE NVTPVKKLA 
      KKKEKKQKE VIENKTEEV MTPAMKEIL AKKEKNALK 
      KKKTKTSDD KNGEVDNAG MMEIRKKKD KASEETNGH 
      LGARKKKTK ASSDDSDGE VYNAEMLEI KQKINKGRS 
      EEVRENGRP RRHLPACNY DERTPRQPR KKT
    CDS sequence
    • >TC012687 RA
      atgtcgaaa ccggtgaaa aaacctcgc aaaaaagca 
      acaaccggc tataaaatg ccggagccg ctgcctgca 
      ggggaagtc ctggttgat gtggggaag aaccagtgg 
      aggttgggg ccgtccatc ggcaaaggg ggctttggg 
      gagatttac gcagctcag gaggcctcc tccaagtcc 
      cccaaatac ccctacgtt gtgaaggtg gagcctcac 
      ggcaacggc ccccttttc gtcgagatg cacttctac 
      atgaaaaac gccaaacag gccgacatt gaggctttt 
      atgaaaaag cacaaattg aaaacgttc ggaatgccg 
      gcgtatttg gggtcaggg tcgcacgaa atgggggct 
      tccaaatac cggtttatt gtcatggag aaattcggg 
      gctgatatt gaccaactg aggcgggac aagggggcc 
      ctttcgagc acgtccgtt tttcaaatg gggtggcaa 
      attacctac gttctggaa tatattcat gactttggg 
      tacatccat ggggacata aaagggggc aatgtcctt 
      ctgggggtc aataaaagc caggtctac ctcgtggat 
      tttggtctc gcgaccaag tttagcaca gagcgggag 
      tttaaaccg aaccccaaa aaagcccac gatggcacc 
      attgagtac accagccgg gacgcccat catggggtg 
      cccaccaga cgtggcgat ttcgaaatt ctgggcttt 
      aatttaatt cattggttg gggggcacc ctcccctgg 
      gactcgcac ttaagcgac cccaaaacc gtccaacag 
      ttgaaggaa gacaattta aaagacgtc ccaaagttt 
      attaaaacg tgcctgggc aagaaatcc gaaacttta 
      atcaaatat ttccaatac gttaatagt ttaaagttt 
      gatgaggtg cctgattat aagaaaatt cggtcgttg 
      tttgaggac gaaattcgc aagaatggc aaaactgtg 
      gacagtcct tttgacttc cttgggaca gtgaaaaaa 
      agaagaagt ttatgtggg gatagattg gatgatgga 
      gatgatgaa aatgtcaca cctgtgaag aaactggcg 
      aaaaaaaag gagaagaaa cagaaggaa gtgatagaa 
      aataagact gaagaggtg atgacaccc gccatgaaa 
      gaaatctta gccaagaag gagaaaaat gcgctcaaa 
      aaaaagaaa accaaaact agtgatgat aaaaatggt 
      gaggttgac aacgccgga atgatggaa ataagaaag 
      aaaaaagac aaagctagt gaagagaca aatggtcat 
      cttggggca cgaaagaag aaaacgaaa gccagtagt 
      gatgatagc gatggcgaa gtatacaat gccgaaatg 
      ctcgaaata aaacagaag attaacaaa ggaagaagt 
      gaagaagtg agagagaac ggaagacct aggcgccat 
      ctcccggct tgtaattac gacgagcga actcccaga 
      caaccgagg aagaagact tag
    mRNA sequence
    • >TC012687 RA
      tccgccata ccaccgttt ttttttcgc gcgtttttc 
      gttatctct tcataattc gttatctcg gatttaaaa 
      ccgcgtttt tgttgttta gtttcgctc taaaggaag 
      tgcaaaaag tgttgcgtg tgtgcgtgg ctcgtgtcg 
      cttcgtttt ttggcgttt gattctaac ctcaaaatg 
      tcgaaaccg gtgaaaaaa cctcgcaaa aaagcaaca 
      accggctat aaaatgccg gagccgctg cctgcaggg 
      gaagtcctg gttgatgtg gggaagaac cagtggagg 
      ttggggccg tccatcggc aaagggggc tttggggag 
      atttacgca gctcaggag gcctcctcc aagtccccc 
      aaatacccc tacgttgtg aaggtggag cctcacggc 
      aacggcccc cttttcgtc gagatgcac ttctacatg 
      aaaaacgcc aaacaggcc gacattgag gcttttatg 
      aaaaagcac aaattgaaa acgttcgga atgccggcg 
      tatttgggg tcagggtcg cacgaaatg ggggcttcc 
      aaataccgg tttattgtc atggagaaa ttcggggct 
      gatattgac caactgagg cgggacaag ggggccctt 
      tcgagcacg tccgttttt caaatgggg tggcaaatt 
      acctacgtt ctggaatat attcatgac tttgggtac 
      atccatggg gacataaaa gggggcaat gtccttctg 
      ggggtcaat aaaagccag gtctacctc gtggatttt 
      ggtctcgcg accaagttt agcacagag cgggagttt 
      aaaccgaac cccaaaaaa gcccacgat ggcaccatt 
      gagtacacc agccgggac gcccatcat ggggtgccc 
      accagacgt ggcgatttc gaaattctg ggctttaat 
      ttaattcat tggttgggg ggcaccctc ccctgggac 
      tcgcactta agcgacccc aaaaccgtc caacagttg 
      aaggaagac aatttaaaa gacgtccca aagtttatt 
      aaaacgtgc ctgggcaag aaatccgaa actttaatc 
      aaatatttc caatacgtt aatagttta aagtttgat 
      gaggtgcct gattataag aaaattcgg tcgttgttt 
      gaggacgaa attcgcaag aatggcaaa actgtggac 
      agtcctttt gacttcctt gggacagtg aaaaaaaga 
      agaagttta tgtggggat agattggat gatggagat 
      gatgaaaat gtcacacct gtgaagaaa ctggcgaaa 
      aaaaaggag aagaaacag aaggaagtg atagaaaat 
      aagactgaa gaggtgatg acacccgcc atgaaagaa 
      atcttagcc aagaaggag aaaaatgcg ctcaaaaaa 
      aagaaaacc aaaactagt gatgataaa aatggtgag 
      gttgacaac gccggaatg atggaaata agaaagaaa 
      aaagacaaa gctagtgaa gagacaaat ggtcatctt 
      ggggcacga aagaagaaa acgaaagcc agtagtgat 
      gatagcgat ggcgaagta tacaatgcc gaaatgctc 
      gaaataaaa cagaagatt aacaaagga agaagtgaa 
      gaagtgaga gagaacgga agacctagg cgccatctc 
      ccggcttgt aattacgac gagcgaact cccagacaa 
      ccgaggaag aagacttag tacctttac tgtttttta 
      cgaattaat aaaaataaa gattttaaa agtaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY