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Gene information
General information
TC012671 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 12821906..12837276
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012671
    iBeetle screen iB_05293

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 8 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012671 RA
      MEDGHTKTV EEVLNYFNT DPERGLTLD QVKRNQEKY 
      GPNELPAEE GKSIWQLVL EQFDDLLVK ILLLAAIIS 
      FVLALFEEH DGAFTAFVE PFVILLILI ANAVVGVWQ 
      ERNAESAIE ALKEYEPEM GKVLRGDKS GVQKIRAKE 
      IVPGDIVEV SVGDKIPAD IRLTKIFST TLRIDQSIL 
      TGESVSVIK HTDAIPDPR AVNQDKKNI LFSGTNVAA 
      GKARGVVVG TGLNTAIGK IRTEMSETE EIKTPLQQK 
      LDEFGEQLS KVISVICVA VWAINIGHF NDPAHGGSW 
      IKGAVYYFK IAVALAVAA IPEGLPAVI TTCLALGTR 
      RMAKKNAIV RSLPSVETL GCTSVICSD KTGTLTTNQ 
      MSVSRMFVF EKVEGSDSS FHEFEITGS TYEPIGEVF 
      LKGQKVKCS EYEGLQELG VICIMCNDS AIDFNEFKQ 
      AFEKVGEAT ETALIVLAE KMNPFQVTK AGDRRQTAI 
      CVRQDIETK WKKEFTLEF SRDRKSMSS YCVPLKPSR 
      LGNGPKLFV KGAPEGVLE RCTHARVGT QKVPLTNTL 
      KNRILDLTK VYGTGRDTL RCLALATGD NPMKPEEMD 
      LGDSTKFYT YEVNLTFVG VVGMLDPPR KEVMDSIAR 
      CRAAGIRVI VITGDNKAT AEAICRRIG VFTEDEDTT 
      GKSFSGREF DDLSPAEQK AACAKARLF SRVEPAHKS 
      KIVEYLQSM NEISAMTGD GVNDAPALK KAEIGIAMG 
      SGTAVAKSA SEMVLADDN FSSIVAAVE EGRAIYNNM 
      KQFIRYLIS SNIGEVVSI FLTAALGLP EALIPVQLL 
      WVNLVTDGL PATALGFNP PDLDIMSKP PRKADESLI 
      SGWLFFRYL AIGGYVGAA TVGAAAWWF MYSPEGPQM 
      NYYQLTHHL QCISGGPEF KGIDCKVFN DPHPMTMAL 
      SVLVTIEML NAMNSLSEN QSLIVMPPW SNWWLMGSM 
      ALSFTLHFV ILYIDVLSV VFQVCPLTG DEWLTVMKF 
      SIPVVLLDE TLKFVARKI TDVGEVVVD KW
    CDS sequence
    • >TC012671 RA
      atggaggac ggacacacc aaaacggtg gaagaagta 
      ttaaactat ttcaatacc gacccagaa cgggggctc 
      accttagat caagtcaaa agaaaccaa gaaaaatat 
      ggacccaat gaacttcca gcggaagaa ggaaagtcc 
      atttggcaa ttagtttta gaacagttc gatgatcta 
      ctagtcaag attttattg ttggccgcc attatttca 
      ttcgttctc gctttattt gaagaacac gatggagct 
      ttcaccgct ttcgtagaa cctttcgtt attcttctc 
      attcttatc gccaatgca gtcgtcggt gtctggcag 
      gaacgaaat gccgaatcg gccattgaa gcgctcaaa 
      gagtacgag cccgaaatg ggcaaagtc ctccgcggc 
      gacaaatcc ggcgtccag aaaatccgg gcgaaagaa 
      atcgtcccc ggagacatc gtcgaggtc tccgtcggc 
      gacaaaatc cccgctgat attcgtcta acaaaaatc 
      ttttcgaca actttgcgc attgatcag tcgattttg 
      accggagaa tcggtctca gtcatcaaa cacaccgac 
      gctattccc gacccacgt gccgtcaac caggacaag 
      aaaaacatc ctcttctcg ggtaccaat gtagcggct 
      ggcaaggca cgtggtgtt gtcgttggc accggcttg 
      aacactgcg atcggtaag attcgtacc gaaatgtcc 
      gaaactgag gaaatcaaa acgccgctg caacaaaaa 
      cttgacgag tttggcgag caattgtcg aaggttatt 
      tctgtgatt tgtgtcgct gtttgggcc atcaatatt 
      gggcatttt aacgatccg gcccatggc gggtcctgg 
      atcaagggt gctgtctat tactttaaa attgccgtt 
      gccctggct gtggctgcg attcccgag ggcttgcct 
      gctgttatt acgacttgt ctggctttg ggcacgcgc 
      cgtatggcc aagaagaac gcaattgtt aggtcacta 
      ccgtctgtt gaaaccctg ggttgcact tcggtcatc 
      tgttcggac aagaccggc actttgacc accaatcaa 
      atgtccgtt tcgcgcatg ttcgtgttc gagaaggtt 
      gagggtagc gatagcagt ttccatgag tttgaaatc 
      accggttcg acgtacgaa ccaatcggc gaggttttc 
      ctcaaaggc cagaaggtc aagtgttct gaatacgaa 
      ggtctgcaa gaacttggc gttatctgc attatgtgc 
      aacgactct gccatcgat ttcaatgag tttaagcaa 
      gcgttcgag aaggtcggt gaagctacc gagactgcg 
      ctgattgtc ctggccgag aagatgaat ccgttccaa 
      gtcaccaag gctggtgat cgtcgccag acggccatt 
      tgcgtgcgc caggacatt gagaccaag tggaagaag 
      gagttcacg ctggagttt tcgcgcgat cgcaaatcg 
      atgtcttcc tattgtgtt cctttgaag ccctcgcgt 
      ctgggtaat ggtcctaag ctgttcgtt aaaggtgcc 
      cctgaaggt gtgctcgag cggtgcacg catgcccgt 
      gttggtacc cagaaagtt cctcttact aacacgctc 
      aagaaccgg attttggat ttgacgaaa gtttacggt 
      actggacgg gacactctc cgttgtctt gcgcttgcg 
      accggcgat aacccgatg aagcccgaa gagatggac 
      ttgggtgat tccaccaaa ttctacact tatgaagtt 
      aatctcacc tttgtgggt gttgtgggg atgttggat 
      cctccacgt aaggaagtt atggattcg attgccagg 
      tgccgggcg gctggtatt cgggttatt gttatcact 
      ggtgataat aaggctact gctgaggct atctgcaga 
      cgtattggt gtctttacg gaagatgag gatacaact 
      ggaaaatct ttctctgga agggaattt gacgatttg 
      agtccggct gaacaaaag gccgcctgt gccaaagcc 
      aggctgttc tcacgtgtg gagcccgct cacaaatcc 
      aagattgtt gaatatttg caaagcatg aacgaaatt 
      tccgctatg actggtgat ggtgtcaac gacgcccca 
      gccttgaag aaggccgag attggcatt gccatgggt 
      tctggaacg gccgtcgct aaatcagcc tctgagatg 
      gtcttggcc gacgataac ttctcgtcc attgtagca 
      gcggttgaa gaaggtcgc gccatttac aacaacatg 
      aaacagttc atccgttac ctgatttcc tcgaacatc 
      ggtgaagtc gtatcaatt ttcttgacg gctgctctt 
      ggtcttccc gaagctttg atccccgta caacttttg 
      tgggtcaat ttggtaact gacggtctc cccgctact 
      gcattaggt ttcaatcca cccgacttg gacatcatg 
      tcaaaaccg cccagaaaa gccgacgaa tcgctcatt 
      tccggctgg ttgttcttc aggtatctc gcaattggt 
      ggctatgtc ggtgctgca actgttggt gctgccgcc 
      tggtggttt atgtactcg cctgaaggc ccacaaatg 
      aattattac caattgact catcacttg caatgcatc 
      agcggtggg cctgaattc aaaggtatc gactgcaag 
      gtcttcaac gatcctcat cccatgacc atggctctc 
      tctgtactc gtaactatt gaaatgctg aacgctatg 
      aacagcttg tctgagaac cagtcgttg attgtcatg 
      cccccatgg tccaactgg tggttgatg ggctcgatg 
      gctctgtcc ttcaccctt cattttgtt attctttac 
      attgatgtc ttatccgtt gtgttccaa gtgtgtcca 
      ttgaccgga gacgagtgg ttaactgta atgaaattc 
      tcaattcca gtagtatta cttgatgaa acgctcaaa 
      ttcgtcgca agaaagatc acagatgtt ggcgaagtt 
      gttgtagat aagtggtaa
    mRNA sequence
    • >TC012671 RA
      gccccgaca aacttcgtc cggcgcccg ttcgtttag 
      cagcttagt acacgttgc gctcatcca tgggggcct 
      aaccacttc gtgttagtt tttatttat acaaagcgt 
      tatcgagtg atacgactg ggaccacaa ttgtgatag 
      cggtaagtt ctagtgcag tgcagtgcc ctcgacgtg 
      gacctgctt gaattgcgt aaaccgcaa attcgggcc 
      ttgccaaaa tcatcggtg ctaaaaccg tccgctagc 
      atgttggca gcacgccca gacccgggc tgtaccaaa 
      ataaaacgc aacttttgc gctaatgac gcactgggt 
      ctccgcttt tcgaaaatc gcgcccgct tcgggcggc 
      ttgattttg gcgcggtgg gacgcacca atcagcggc 
      gtccacatg ccgattgtt tgacaaaac tgtcaaaag 
      cggaaagtc aacgtttat ttaattcag tttgagttg 
      cgagcacga ttttggcca aattttcgg caaaacacg 
      ctgtttaat tggaggtag agtgaggtt agacgtgga 
      aaagcgcct gtcacgctg cgcagctcg cgaacgtgg 
      ctcacggct cccggtaat gaggctgta actcacgat 
      agtgtggct gctgaacga tttcggtga gaaaatgct 
      ggtgttgtg cgactcatt ttcggtgaa acactgagt 
      tttgtagcg cttttgccc gttttcctc tcgcgagtt 
      ttaggttat agaatacgt aaatattta atagttgat 
      gcacgtttc ctgtgtgaa gtgactgac aaacaccat 
      ggaggacgg acacaccaa aacggtgga agaagtatt 
      aaactattt caataccga cccagaacg ggggctcac 
      cttagatca agtcaaaag aaaccaaga aaaatatgg 
      acccaatga acttccagc ggaagaagg aaagtccat 
      ttggcaatt agttttaga acagttcga tgatctact 
      agtcaagat tttattgtt ggccgccat tatttcatt 
      cgttctcgc tttatttga agaacacga tggagcttt 
      caccgcttt cgtagaacc tttcgttat tcttctcat 
      tcttatcgc caatgcagt cgtcggtgt ctggcagga 
      acgaaatgc cgaatcggc cattgaagc gctcaaaga 
      gtacgagcc cgaaatggg caaagtcct ccgcggcga 
      caaatccgg cgtccagaa aatccgggc gaaagaaat 
      cgtccccgg agacatcgt cgaggtctc cgtcggcga 
      caaaatccc cgctgatat tcgtctaac aaaaatctt 
      ttcgacaac tttgcgcat tgatcagtc gattttgac 
      cggagaatc ggtctcagt catcaaaca caccgacgc 
      tattcccga cccacgtgc cgtcaacca ggacaagaa 
      aaacatcct cttctcggg taccaatgt agcggctgg 
      caaggcacg tggtgttgt cgttggcac cggcttgaa 
      cactgcgat cggtaagat tcgtaccga aatgtccga 
      aactgagga aatcaaaac gccgctgca acaaaaact 
      tgacgagtt tggcgagca attgtcgaa ggttatttc 
      tgtgatttg tgtcgctgt ttgggccat caatattgg 
      gcattttaa cgatccggc ccatggcgg gtcctggat 
      caagggtgc tgtctatta ctttaaaat tgccgttgc 
      cctggctgt ggctgcgat tcccgaggg cttgcctgc 
      tgttattac gacttgtct ggctttggg cacgcgccg 
      tatggccaa gaagaacgc aattgttag gtcactacc 
      gtctgttga aaccctggg ttgcacttc ggtcatctg 
      ttcggacaa gaccggcac tttgaccac caatcaaat 
      gtccgtttc gcgcatgtt cgtgttcga gaaggttga 
      gggtagcga tagcagttt ccatgagtt tgaaatcac 
      cggttcgac gtacgaacc aatcggcga ggttttcct 
      caaaggcca gaaggtcaa gtgttctga atacgaagg 
      tctgcaaga acttggcgt tatctgcat tatgtgcaa 
      cgactctgc catcgattt caatgagtt taagcaagc 
      gttcgagaa ggtcggtga agctaccga gactgcgct 
      gattgtcct ggccgagaa gatgaatcc gttccaagt 
      caccaaggc tggtgatcg tcgccagac ggccatttg 
      cgtgcgcca ggacattga gaccaagtg gaagaagga 
      gttcacgct ggagttttc gcgcgatcg caaatcgat 
      gtcttccta ttgtgttcc tttgaagcc ctcgcgtct 
      gggtaatgg tcctaagct gttcgttaa aggtgcccc 
      tgaaggtgt gctcgagcg gtgcacgca tgcccgtgt 
      tggtaccca gaaagttcc tcttactaa cacgctcaa 
      gaaccggat tttggattt gacgaaagt ttacggtac 
      tggacggga cactctccg ttgtcttgc gcttgcgac 
      cggcgataa cccgatgaa gcccgaaga gatggactt 
      gggtgattc caccaaatt ctacactta tgaagttaa 
      tctcacctt tgtgggtgt tgtggggat gttggatcc 
      tccacgtaa ggaagttat ggattcgat tgccaggtg 
      ccgggcggc tggtattcg ggttattgt tatcactgg 
      tgataataa ggctactgc tgaggctat ctgcagacg 
      tattggtgt ctttacgga agatgagga tacaactgg 
      aaaatcttt ctctggaag ggaatttga cgatttgag 
      tccggctga acaaaaggc cgcctgtgc caaagccag 
      gctgttctc acgtgtgga gcccgctca caaatccaa 
      gattgttga atatttgca aagcatgaa cgaaatttc 
      cgctatgac tggtgatgg tgtcaacga cgccccagc 
      cttgaagaa ggccgagat tggcattgc catgggttc 
      tggaacggc cgtcgctaa atcagcctc tgagatggt 
      cttggccga cgataactt ctcgtccat tgtagcagc 
      ggttgaaga aggtcgcgc catttacaa caacatgaa 
      acagttcat ccgttacct gatttcctc gaacatcgg 
      tgaagtcgt atcaatttt cttgacggc tgctcttgg 
      tcttcccga agctttgat ccccgtaca acttttgtg 
      ggtcaattt ggtaactga cggtctccc cgctactgc 
      attaggttt caatccacc cgacttgga catcatgtc 
      aaaaccgcc cagaaaagc cgacgaatc gctcatttc 
      cggctggtt gttcttcag gtatctcgc aattggtgg 
      ctatgtcgg tgctgcaac tgttggtgc tgccgcctg 
      gtggtttat gtactcgcc tgaaggccc acaaatgaa 
      ttattacca attgactca tcacttgca atgcatcag 
      cggtgggcc tgaattcaa aggtatcga ctgcaaggt 
      cttcaacga tcctcatcc catgaccat ggctctctc 
      tgtactcgt aactattga aatgctgaa cgctatgaa 
      cagcttgtc tgagaacca gtcgttgat tgtcatgcc 
      cccatggtc caactggtg gttgatggg ctcgatggc 
      tctgtcctt cacccttca ttttgttat tctttacat 
      tgatgtctt atccgttgt gttccaagt gtgtccatt 
      gaccggaga cgagtggtt aactgtaat gaaattctc 
      aattccagt agtattact tgatgaaac gctcaaatt 
      cgtcgcaag aaagatcac agatgttgg cgaagttgt 
      tgtagataa gtggtaact cttcggtcg atcgatctt 
      cacgctttt ttattatat ttctagagt aatttaatt 
      ttagaagac ttgcagcta tttattttt tcacctaac 
      tcgattata cgatttttt tgctacagt agaattgca 
      tgcaagaca tgaggtact ttactttta atttcgaga 
      aagctttat ttgcataag tttactaaa atcatcttg 
      tgttgtttt gtcgaaatc atccacctt ttgtcttcc 
      cggagacct gtgatggtg ttagaggcg cattagaaa 
      ctaactaac gaaacaatg tacataaaa taatataag 
      agaatttca agattgtta ttgatttgt gagcagagc 
      tcgttttgg agctttgtt atccttgtg gactattca 
      cagacgaga tcagcaccg accgggatc atttagaaa 
      gtgagagag gaggatgta gccttctcg acttcccct 
      gagtgttgt cacaatttt tgaaattaa tcaacgtat 
      tcgttagtt tagttcact tttgccacg tgtgtgttt 
      cgtggcctc atcgttcat gtgtggctc ggttcgagc 
      tggtctcga ctgaggacg ggctgcgct ggttccggc 
      agcccccat gccattgta tagttctac atttaattc 
      acatcagtt ttgtaaaca ggatatttg tgtaaataa 
      agtgatggc aacggctat cttaactta cacgcgcct 
      ctcttctca acccttttt ctactcctt ttttcccaa 
      ctacaacaa taaacaggt tattttcct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY