| General information | |||
| TC034427
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 12755116..12778605 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC034427 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05292 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)Days post injection
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
Days post injection
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Days post injection
Fertility
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (16 dpi)
Days post injection
eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 1 (16 dpi)
Days post injection
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (19 dpi)
Days post injection
Pupal morphology
head orientation irregular -
Number of animals showing the phenotype: 2
legs partially orientation irregular - Number of animals showing the phenotype: 2
legs partially orientation irregular - Number of animals showing the phenotype: 2
- >TC034427 RA
MPAQPNMNM RRVFLNRKM ATDLSVDNA NGNDHHPTM MNLTIPKPR SAVTTSSQL QTNTQSSTT TTRVTSSQR VHVTSSDLK ASNMKSDLI EMKNNLNDM KSSISNLNK LRSSLENLA DDDDDMAEM AQPLVTFPD DTPVPSEVG SPTRTVDTV KFEQKTMNN FKKTKVVKD GISAEKESA KCAEMKKIQ AGDVSYEEN SAAASSRAR LEIDGISAE KSQMKAKET RSLKAGDLS QQESKNMAQ TNMKVKTDK FYTEQTARE SEQQRQTVT SSGVYNSEK HTSSSHSYT TTTKGISTS AATTLRQIS LLNGNKEEE ILNMTCEDL EQLSTTSNP QEVERAIHK YSSYLDQSI KCLQKTDQA KNAPLILEK INDVMQKAW AVPTHGHEL GYSLCNALR NCGGLDLLM TNCTKTDKK LQFASARLL EQCLTTENR AHVVEHGLD KVVNIACAC TKNSNSVDH SRVGTGILE HLFKHSEET CSDVVRLGG LDALLFECR KSDVETLRH CAGALANLS LYGGAENQE AMINRKVPM WLFPLAFHH DDNIKYYAC LAITVLVAN AEIEAQVLQ SGTLGLVEP FVTSHNPSE FARSNLAHA HGQSKTWLK HLVPVLSSK REEARNLAA FHFCMEAGI KAQQGNTNI FAEIGAIES LKKVASCPN AVASKYAAQ ALRLIGEEV PHKLSQQVP LWSAEDVEE WVKQIGFAQ YAPSFVESR VDGDLLLQL TEENLREDI GLTNGIKRK RFTRELQKL KKMADYTSR DTANINTFL QTLGPEFSI YTYSLLNAG VETKEALRN ISEDQLLNE CTITNSIHR FRIMEGIRQ LENGLANLN EDNLDKSLD VFVSYRRSN GSQLASLLK VHLQLRGFS VFIDVERLE AGKFDNNLL QSIQKAKHF LLVLTPNAL ERCRGDTER KDWVHREIV AALQAQCNI IPIIDNFQW PEADELPED MRQVCHFNG VRWIHDYQD ACVDKLERF MRGELSTRC TEGVKFGKG DNTPGTPAN PVLARQPPS YQRMHSNDS GSGKGSDKD VNGNTGLSR D
- >TC034427 RB
MPAQPNMNM RRVFLNRKC YSLPAREQT RRHVNESVR ILLKMYSAN RGITHSFYH ARPIGIQER IKFFKEREQ MATDLSVDN ANGNDHHPT MMNLTIPKP RSAVTTSSQ LQTNTQSST TTTRVTSSQ RVHVTSSDL KASNMKSDL IEMKNNLND MKSSISNLN KLRSSLENL ADDDDDMAE MAQPLVTFP DDTPVPSEV GSPTRTVDT VKFEQKTMN NFKKTKVVK DGISAEKES AKCAEMKKI QAGDVSYEE NSAAASSRA RLEIDGISA EKSQMKAKE TRSLKAGDL SQQESKNMA QTNMKVKTD KFYTEQTAR ESEQQRQTV TSSGVYNSE KHTSSSHSY TTTTKGIST SAATTLRQI SLLNGNKEE EILNMTCED LEQLSTTSN PQEVERAIH KYSSYLDQS IKCLQKTDQ AKNAPLILE KINDVMQKA WAVPTHGHE LGYSLCNAL RNCGGLDLL MTNCTKTDK KLQFASARL LEQCLTTEN RAHVVEHGL DKVVNIACA CTKNSNSVD HSRVGTGIL EHLFKHSEE TCSDVVRLG GLDALLFEC RKSDVETLR HCAGALANL SLYGGAENQ EAMINRKVP MWLFPLAFH HDDNIKYYA CLAITVLVA NAEIEAQVL QSGTLGLVE PFVTSHNPS EFARSNLAH AHGQSKTWL KHLVPVLSS KREEARNLA AFHFCMEAG IKAQQGNTN IFAEIGAIE SLKKVASCP NAVASKYAA QALRLIGEE VPHKLSQQV PLWSAEDVE EWVKQIGFA QYAPSFVES RVDGDLLLQ LTEENLRED IGLTNGIKR KRFTRELQK LKKMADYTS RDTANINTF LQTLGPEFS IYTYSLLNA GVETKEALR NISEDQLLN ECTITNSIH RFRIMEGIR QLENGLANL NEDNLDKSL DVFVSYRRS NGSQLASLL KVHLQLRGF SVFIDVERL EAGKFDNNL LQSIQKAKH FLLVLTPNA LERCRGDTE RKDWVHREI VAALQAQCN IIPIIDNFQ WPEADELPE DMRQVCHFN GVRWIHDYQ DACVDKLER FMRGELSTR CTEGVKFGK GDNTPGTPA NPVLARQPP SYQRMHSND SGSGKGSDK DVNGNTGLS RD
- >TC034427 RA
atgcctgcc cagccaaat atgaacatg cgtcgggtc ttcctgaac cgaaagatg gcgaccgat ttgtccgtc gacaatgcc aacggaaac gatcatcat cccacgatg atgaacctg acgatcccg aagccgagg tccgcagtg accacatcc tcgcagttg cagacgaat acgcaaagc agtacgaca acgacacgc gttacctct tcgcaacga gtccatgtg acctcctcc gacctgaag gcatctaac atgaaatct gatctcatc gaaatgaag aacaatctg aacgacatg aagagcagc atatcgaat ctgaacaag ttgcggagc tcgctggag aacctcgcc gacgacgac gacgacatg gccgagatg gcgcagccc ttggtcacc ttccccgac gacacgccg gtgccctcg gaggtgggc tcgcccacg aggacggtc gacaccgtc aagttcgag cagaagacc atgaacaac ttcaaaaag acaaaggtt gtgaaagac gggataagc gccgagaag gagagtgca aaatgtgcc gaaatgaag aagatccaa gccggggac gtgtcctac gaggagaac agcgcagcg gcgtcttcg agggcccgt ctggaaatc gacggtatc tcggcggag aagagccaa atgaaagct aaggagaca cggtcactg aaagctgga gatcttagc caacaagag tcgaaaaat atggcacag actaatatg aaagtcaaa actgataaa ttctatacg gagcaaaca gcaagggaa agtgaacaa caacgacaa acggtcacc tctagtggt gtttacaat tccgaaaaa cacacctca tctagtcac tcctacacg accaccacc aaaggaatt agcacttcg gcagccacg acgttgcga cagatcagt ttactgaac ggcaacaaa gaagaagag atcctcaac atgacctgc gaggacctc gagcagctc tccactacg tccaaccca caagaagtg gagcgcgcc atccacaag tactcctcg tacctcgat cagtcaatt aaatgcctc cagaagacc gaccaagcg aaaaacgcc cccctaatc ctcgagaaa atcaacgac gtgatgcag aaagcctgg gccgtgccg acccacggc cacgaactg ggctattcc ctctgtaac gcgctccgc aactgcggc ggcctggac ctcctcatg acaaactgc accaaaacc gacaagaaa ctccaattc gcgagcgcc cgcctccta gaacaatgc ctaaccacc gaaaacagg gcccacgtc gtcgaacac ggcctcgac aaagtggtc aacatcgcc tgcgcttgc acgaaaaac tccaactcc gtggaccac tcccgcgtc ggcacgggc atcctggag cacctgttc aagcacagc gaggaaacc tgcagcgac gtggtcagg ctgggcggc ctggacgcc ctcctcttc gaatgccgg aagagcgac gtggagact ttgcggcac tgcgctggg gctttggcc aacttgagt ttatacgga ggggcggag aaccaggaa gcgatgatc aatcgcaag gtgccgatg tggctgttc ccgctggcc ttccaccac gacgacaac atcaaatac tacgcctgt ttggcgatc actgtcctg gtggcgaac gcggaaatc gaggcccaa gtgctccag tcgggcacc ttggggctc gtcgagccc ttcgtcacg tcgcacaac ccctccgag ttcgccagg tcgaacctg gcgcacgca cacggccag agcaagacg tggctcaag cacctggtg ccggtcttg agctccaag cgggaggag gcgcgcaat ttggccgcc ttccacttt tgcatggaa gcggggatc aaagcacag caggggaac acgaatatt tttgccgaa attggcgcg attgaatcg ctcaaaaaa gtggcaagt tgtccgaac gccgtggcg tcgaaatac gcagctcaa gccctgagg ctgatcggc gaggaagtg ccccacaag ctgagccaa caagtgccc ctgtggtcg gctgaggac gtggaagag tgggtcaaa cagatcggg ttcgcacaa tacgccccc agttttgtt gaaagtcgg gtcgatggc gatcttttg ctccaacta acggaggag aatttacga gaggatatc gggctgacg aacggaatc aagcgaaag cggtttacc cgcgagttg caaaagttg aagaaaatg gctgattat acgtcgagg gacacggcc aatattaac acgtttttg caaacgctg ggtccggag tttagtatt tacacgtat agtttgctg aatgcggga gttgagacc aaagaagcg ctgaggaat atttccgag gatcagttg ctgaatgag tgcacaatc acgaattcg attcaccgg tttcggatc atggaaggg atcaggcag ctggaaaat gggcttgcg aatttgaat gaggataat ctggacaag tcgttggac gtgtttgtc agttatcga aggtcgaac ggatctcaa ttagctagt ttattaaaa gtacattta caattaagg ggattcagt gtttttatc gatgtcgag cggctggaa gcagggaaa ttcgataat aatctgctg caaagtatt caaaaagcc aagcatttc ttgctggtg ctcacgccg aatgcgctc gagaggtgc cgaggggac actgaacgg aaagactgg gtgcataga gagattgtg gcagctttg caagctcag tgtaacatt attcctatc attgataat tttcaatgg ccggaagcg gacgaatta cccgaagat atgaggcaa gtgtgtcac tttaacgga gttcggtgg attcatgac tatcaggac gcttgcgtg gacaaatta gaaaggttc atgcgaggc gaactgagc acacgttgc accgaaggc gtgaagttt ggtaagggc gacaacacg ccgggaacc cccgcgaac ccggtcctg gcccgacag ccgcccagt taccagcga atgcacagt aacgactcc ggcagtggc aaaggctcc gacaaggac gtgaacggc aacacggga ctctctagg gattga
- >TC034427 RB
atgcctgcc cagccaaat atgaacatg cgtcgggtc ttcctgaac cgaaagtgc tattcctta cctgcgagg gagcagacg cgtcgccat gtgaatgaa agtgttcga attttgctc aaaatgtac tcagccaat cggggaatc acgcacagc ttctaccac gcgcggccg atcggcatc caagaacgg attaaattt ttcaaagag cgggaacag atggcgacc gatttgtcc gtcgacaat gccaacgga aacgatcat catcccacg atgatgaac ctgacgatc ccgaagccg aggtccgca gtgaccaca tcctcgcag ttgcagacg aatacgcaa agcagtacg acaacgaca cgcgttacc tcttcgcaa cgagtccat gtgacctcc tccgacctg aaggcatct aacatgaaa tctgatctc atcgaaatg aagaacaat ctgaacgac atgaagagc agcatatcg aatctgaac aagttgcgg agctcgctg gagaacctc gccgacgac gacgacgac atggccgag atggcgcag cccttggtc accttcccc gacgacacg ccggtgccc tcggaggtg ggctcgccc acgaggacg gtcgacacc gtcaagttc gagcagaag accatgaac aacttcaaa aagacaaag gttgtgaaa gacgggata agcgccgag aaggagagt gcaaaatgt gccgaaatg aagaagatc caagccggg gacgtgtcc tacgaggag aacagcgca gcggcgtct tcgagggcc cgtctggaa atcgacggt atctcggcg gagaagagc caaatgaaa gctaaggag acacggtca ctgaaagct ggagatctt agccaacaa gagtcgaaa aatatggca cagactaat atgaaagtc aaaactgat aaattctat acggagcaa acagcaagg gaaagtgaa caacaacga caaacggtc acctctagt ggtgtttac aattccgaa aaacacacc tcatctagt cactcctac acgaccacc accaaagga attagcact tcggcagcc acgacgttg cgacagatc agtttactg aacggcaac aaagaagaa gagatcctc aacatgacc tgcgaggac ctcgagcag ctctccact acgtccaac ccacaagaa gtggagcgc gccatccac aagtactcc tcgtacctc gatcagtca attaaatgc ctccagaag accgaccaa gcgaaaaac gccccccta atcctcgag aaaatcaac gacgtgatg cagaaagcc tgggccgtg ccgacccac ggccacgaa ctgggctat tccctctgt aacgcgctc cgcaactgc ggcggcctg gacctcctc atgacaaac tgcaccaaa accgacaag aaactccaa ttcgcgagc gcccgcctc ctagaacaa tgcctaacc accgaaaac agggcccac gtcgtcgaa cacggcctc gacaaagtg gtcaacatc gcctgcgct tgcacgaaa aactccaac tccgtggac cactcccgc gtcggcacg ggcatcctg gagcacctg ttcaagcac agcgaggaa acctgcagc gacgtggtc aggctgggc ggcctggac gccctcctc ttcgaatgc cggaagagc gacgtggag actttgcgg cactgcgct ggggctttg gccaacttg agtttatac ggaggggcg gagaaccag gaagcgatg atcaatcgc aaggtgccg atgtggctg ttcccgctg gccttccac cacgacgac aacatcaaa tactacgcc tgtttggcg atcactgtc ctggtggcg aacgcggaa atcgaggcc caagtgctc cagtcgggc accttgggg ctcgtcgag cccttcgtc acgtcgcac aacccctcc gagttcgcc aggtcgaac ctggcgcac gcacacggc cagagcaag acgtggctc aagcacctg gtgccggtc ttgagctcc aagcgggag gaggcgcgc aatttggcc gccttccac ttttgcatg gaagcgggg atcaaagca cagcagggg aacacgaat atttttgcc gaaattggc gcgattgaa tcgctcaaa aaagtggca agttgtccg aacgccgtg gcgtcgaaa tacgcagct caagccctg aggctgatc ggcgaggaa gtgccccac aagctgagc caacaagtg cccctgtgg tcggctgag gacgtggaa gagtgggtc aaacagatc gggttcgca caatacgcc cccagtttt gttgaaagt cgggtcgat ggcgatctt ttgctccaa ctaacggag gagaattta cgagaggat atcgggctg acgaacgga atcaagcga aagcggttt acccgcgag ttgcaaaag ttgaagaaa atggctgat tatacgtcg agggacacg gccaatatt aacacgttt ttgcaaacg ctgggtccg gagtttagt atttacacg tatagtttg ctgaatgcg ggagttgag accaaagaa gcgctgagg aatatttcc gaggatcag ttgctgaat gagtgcaca atcacgaat tcgattcac cggtttcgg atcatggaa gggatcagg cagctggaa aatgggctt gcgaatttg aatgaggat aatctggac aagtcgttg gacgtgttt gtcagttat cgaaggtcg aacggatct caattagct agtttatta aaagtacat ttacaatta aggggattc agtgttttt atcgatgtc gagcggctg gaagcaggg aaattcgat aataatctg ctgcaaagt attcaaaaa gccaagcat ttcttgctg gtgctcacg ccgaatgcg ctcgagagg tgccgaggg gacactgaa cggaaagac tgggtgcat agagagatt gtggcagct ttgcaagct cagtgtaac attattcct atcattgat aattttcaa tggccggaa gcggacgaa ttacccgaa gatatgagg caagtgtgt cactttaac ggagttcgg tggattcat gactatcag gacgcttgc gtggacaaa ttagaaagg ttcatgcga ggcgaactg agcacacgt tgcaccgaa ggcgtgaag tttggtaag ggcgacaac acgccggga acccccgcg aacccggtc ctggcccga cagccgccc agttaccag cgaatgcac agtaacgac tccggcagt ggcaaaggc tccgacaag gacgtgaac ggcaacacg ggactctct agggattga
- >TC034427 RA
gagaatgaa aaaatgctt ggcaaatct tcaaagctt tttatttta gttttatcg ttttattgg cgaaaataa cgtcatttt acggatgtt catgtgaaa gtattttta agttgttct tttgcggcc gcaccagat gtcgcctca tctcgaaac tgaatagtt ttcgtgtta ttgagaacg tgtgctcct cgctactaa aattgtaaa caaaatgac ctaaaagca ccatcaaaa tccataact aaagaataa tttatgata ataaaaaat ctaacacac tctcgtctc atttttccc attcttcca tagctctaa cattatttt tcttggcct tgcttggat tatttgcct tttctactt gggttattt ataagtttc ggtgtggag gaaaaaact aatcaagtg gacggccaa ttacataat aacacacct aacagtgca catctgcta ctaaaccat taccataag taaaaagaa tggacggga aaattgtcc ccgaaagac acaagcgga aacaaaaga atcccgatt tgaaactta agtaagcaa cggttgcgc gttcgagtc gcttgtgaa gtcactgaa atttcagca acttctgct atgtgtttt tgtgtcaaa taggccggt gtggtgata ttgtgtcaa gcatggttg tgaataaaa ttacaatgc ctgcccagc caaatatga acatgcgtc gggtcttcc tgaaccgaa agatggcga ccgatttgt ccgtcgaca atgccaacg gaaacgatc atcatccca cgatgatga acctgacga tcccgaagc cgaggtccg cagtgacca catcctcgc agttgcaga cgaatacgc aaagcagta cgacaacga cacgcgtta cctcttcgc aacgagtcc atgtgacct cctccgacc tgaaggcat ctaacatga aatctgatc tcatcgaaa tgaagaaca atctgaacg acatgaaga gcagcatat cgaatctga acaagttgc ggagctcgc tggagaacc tcgccgacg acgacgacg acatggccg agatggcgc agcccttgg tcaccttcc ccgacgaca cgccggtgc cctcggagg tgggctcgc ccacgagga cggtcgaca ccgtcaagt tcgagcaga agaccatga acaacttca aaaagacaa aggttgtga aagacggga taagcgccg agaaggaga gtgcaaaat gtgccgaaa tgaagaaga tccaagccg gggacgtgt cctacgagg agaacagcg cagcggcgt cttcgaggg cccgtctgg aaatcgacg gtatctcgg cggagaaga gccaaatga aagctaagg agacacggt cactgaaag ctggagatc ttagccaac aagagtcga aaaatatgg cacagacta atatgaaag tcaaaactg ataaattct atacggagc aaacagcaa gggaaagtg aacaacaac gacaaacgg tcacctcta gtggtgttt acaattccg aaaaacaca cctcatcta gtcactcct acacgacca ccaccaaag gaattagca cttcggcag ccacgacgt tgcgacaga tcagtttac tgaacggca acaaagaag aagagatcc tcaacatga cctgcgagg acctcgagc agctctcca ctacgtcca acccacaag aagtggagc gcgccatcc acaagtact cctcgtacc tcgatcagt caattaaat gcctccaga agaccgacc aagcgaaaa acgcccccc taatcctcg agaaaatca acgacgtga tgcagaaag cctgggccg tgccgaccc acggccacg aactgggct attccctct gtaacgcgc tccgcaact gcggcggcc tggacctcc tcatgacaa actgcacca aaaccgaca agaaactcc aattcgcga gcgcccgcc tcctagaac aatgcctaa ccaccgaaa acagggccc acgtcgtcg aacacggcc tcgacaaag tggtcaaca tcgcctgcg cttgcacga aaaactcca actccgtgg accactccc gcgtcggca cgggcatcc tggagcacc tgttcaagc acagcgagg aaacctgca gcgacgtgg tcaggctgg gcggcctgg acgccctcc tcttcgaat gccggaaga gcgacgtgg agactttgc ggcactgcg ctggggctt tggccaact tgagtttat acggagggg cggagaacc aggaagcga tgatcaatc gcaaggtgc cgatgtggc tgttcccgc tggccttcc accacgacg acaacatca aatactacg cctgtttgg cgatcactg tcctggtgg cgaacgcgg aaatcgagg cccaagtgc tccagtcgg gcaccttgg ggctcgtcg agcccttcg tcacgtcgc acaacccct ccgagttcg ccaggtcga acctggcgc acgcacacg gccagagca agacgtggc tcaagcacc tggtgccgg tcttgagct ccaagcggg aggaggcgc gcaatttgg ccgccttcc acttttgca tggaagcgg ggatcaaag cacagcagg ggaacacga atatttttg ccgaaattg gcgcgattg aatcgctca aaaaagtgg caagttgtc cgaacgccg tggcgtcga aatacgcag ctcaagccc tgaggctga tcggcgagg aagtgcccc acaagctga gccaacaag tgcccctgt ggtcggctg aggacgtgg aagagtggg tcaaacaga tcgggttcg cacaatacg cccccagtt ttgttgaaa gtcgggtcg atggcgatc ttttgctcc aactaacgg aggagaatt tacgagagg atatcgggc tgacgaacg gaatcaagc gaaagcggt ttacccgcg agttgcaaa agttgaaga aaatggctg attatacgt cgagggaca cggccaata ttaacacgt ttttgcaaa cgctgggtc cggagttta gtatttaca cgtatagtt tgctgaatg cgggagttg agaccaaag aagcgctga ggaatattt ccgaggatc agttgctga atgagtgca caatcacga attcgattc accggtttc ggatcatgg aagggatca ggcagctgg aaaatgggc ttgcgaatt tgaatgagg ataatctgg acaagtcgt tggacgtgt ttgtcagtt atcgaaggt cgaacggat ctcaattag ctagtttat taaaagtac atttacaat taaggggat tcagtgttt ttatcgatg tcgagcggc tggaagcag ggaaattcg ataataatc tgctgcaaa gtattcaaa aagccaagc atttcttgc tggtgctca cgccgaatg cgctcgaga ggtgccgag gggacactg aacggaaag actgggtgc atagagaga ttgtggcag ctttgcaag ctcagtgta acattattc ctatcattg ataattttc aatggccgg aagcggacg aattacccg aagatatga ggcaagtgt gtcacttta acggagttc ggtggattc atgactatc aggacgctt gcgtggaca aattagaaa ggttcatgc gaggcgaac tgagcacac gttgcaccg aaggcgtga agtttggta agggcgaca acacgccgg gaacccccg cgaacccgg tcctggccc gacagccgc ccagttacc agcgaatgc acagtaacg actccggca gtggcaaag gctccgaca aggacgtga acggcaaca cgggactct ctagggatt gactagatg gtccccttg cctcgggcc ccccaactc cgctgccaa ggtgagtcc cagggtgac tgcacgacc cccttgctg cttacgact ttcattatt tttgtgcat atttgagcc gctgatgta actttccct ttttctatt aaccgctcc ccgactaac gtaaatatt tcagttctt tcttgcagg ttttataag accgctagt ccgtcgcgg aactggatt atgaacacg agtcgatac ccgcggggc ttgcccccg cagtacccc tacaaccgg cgcggttcc aacagcccg tcgggcctc ctgggggcc gggaagccc cccaccccg aataggtct cgcagtctc gatggcctt ctggactcg gaaccgact tgtgcagct tcagacgca actataagc gaacccact ccgtcccaa aacacgaaa agttgtgac gatttagat aaagatttc gacgaacag actgaagac gttaattta agtgtaaat aacctggac gtcgataaa gtttcgata cagagcggt tcaagtgat tcgaaacga aaacgaaat ttcatggac cgatgtgtc agtaaagtg agatctctt attaaaaaa taagtcgct ggcgtgtaa actatatta gtgaattat tgtattttc gtacacatg atgtcaaag tgatataat tttatttat tattgtacc tatttgtta tttataatt gctggattc cgaatccgt tttaatgtg tgagggact gcttgcgaa agcgagccc tttttattt ttttaacgg caactaaat tacttagta caaaattgt gcttttata tgaaatgtg atcattatg cagcctttt aaagtcgct atgacttat ttttcgaca ctttattag ggtaaggag tacattaaa ataatgtat aaggtttca tgtttaata cgtacagtt tattgtacc aagcttgcc tcagtaagc agtattggt aacgttctg acaatgcat cattgcctt cttgttcga aaatatcga tgtgcggaa ttaacacat ttactagta ttgtaatgt tgtttttta tgttatatt taaaaatat attttatta agacac
- >TC034427 RB
gagaatgaa aaaatgctt ggcaaatct tcaaagctt tttatttta gttttatcg ttttattgg cgaaaataa cgtcatttt acggatgtt catgtgaaa gtattttta agttgttct tttgcggcc gcaccagat gtcgcctca tctcgaaac tgaatagtt ttcgtgtta ttgagaacg tgtgctcct cgctactaa aattgtaaa caaaatgac ctaaaagca ccatcaaaa tccataact aaagaataa tttatgata ataaaaaat ctaacacac tctcgtctc atttttccc attcttcca tagctctaa cattatttt tcttggcct tgcttggat tatttgcct tttctactt gggttattt ataagtttc ggtgtggag gaaaaaact aatcaagtg gacggccaa ttacataat aacacacct aacagtgca catctgcta ctaaaccat taccataag taaaaagaa tggacggga aaattgtcc ccgaaagac acaagcgga aacaaaaga atcccgatt tgaaactta agtaagcaa cggttgcgc gttcgagtc gcttgtgaa gtcactgaa atttcagca acttctgct atgtgtttt tgtgtcaaa taggccggt gtggtgata ttgtgtcaa gcatggttg tgaataaaa ttacaatgc ctgcccagc caaatatga acatgcgtc gggtcttcc tgaaccgaa agtgctatt ccttacctg cgagggagc agacgcgtc gccatgtga atgaaagtg ttcgaattt tgctcaaaa tgtactcag ccaatcggg gaatcacgc acagcttct accacgcgc ggccgatcg gcatccaag aacggatta aatttttca aagagcggg aacagatgg cgaccgatt tgtccgtcg acaatgcca acggaaacg atcatcatc ccacgatga tgaacctga cgatcccga agccgaggt ccgcagtga ccacatcct cgcagttgc agacgaata cgcaaagca gtacgacaa cgacacgcg ttacctctt cgcaacgag tccatgtga cctcctccg acctgaagg catctaaca tgaaatctg atctcatcg aaatgaaga acaatctga acgacatga agagcagca tatcgaatc tgaacaagt tgcggagct cgctggaga acctcgccg acgacgacg acgacatgg ccgagatgg cgcagccct tggtcacct tccccgacg acacgccgg tgccctcgg aggtgggct cgcccacga ggacggtcg acaccgtca agttcgagc agaagacca tgaacaact tcaaaaaga caaaggttg tgaaagacg ggataagcg ccgagaagg agagtgcaa aatgtgccg aaatgaaga agatccaag ccggggacg tgtcctacg aggagaaca gcgcagcgg cgtcttcga gggcccgtc tggaaatcg acggtatct cggcggaga agagccaaa tgaaagcta aggagacac ggtcactga aagctggag atcttagcc aacaagagt cgaaaaata tggcacaga ctaatatga aagtcaaaa ctgataaat tctatacgg agcaaacag caagggaaa gtgaacaac aacgacaaa cggtcacct ctagtggtg tttacaatt ccgaaaaac acacctcat ctagtcact cctacacga ccaccacca aaggaatta gcacttcgg cagccacga cgttgcgac agatcagtt tactgaacg gcaacaaag aagaagaga tcctcaaca tgacctgcg aggacctcg agcagctct ccactacgt ccaacccac aagaagtgg agcgcgcca tccacaagt actcctcgt acctcgatc agtcaatta aatgcctcc agaagaccg accaagcga aaaacgccc ccctaatcc tcgagaaaa tcaacgacg tgatgcaga aagcctggg ccgtgccga cccacggcc acgaactgg gctattccc tctgtaacg cgctccgca actgcggcg gcctggacc tcctcatga caaactgca ccaaaaccg acaagaaac tccaattcg cgagcgccc gcctcctag aacaatgcc taaccaccg aaaacaggg cccacgtcg tcgaacacg gcctcgaca aagtggtca acatcgcct gcgcttgca cgaaaaact ccaactccg tggaccact cccgcgtcg gcacgggca tcctggagc acctgttca agcacagcg aggaaacct gcagcgacg tggtcaggc tgggcggcc tggacgccc tcctcttcg aatgccgga agagcgacg tggagactt tgcggcact gcgctgggg ctttggcca acttgagtt tatacggag gggcggaga accaggaag cgatgatca atcgcaagg tgccgatgt ggctgttcc cgctggcct tccaccacg acgacaaca tcaaatact acgcctgtt tggcgatca ctgtcctgg tggcgaacg cggaaatcg aggcccaag tgctccagt cgggcacct tggggctcg tcgagccct tcgtcacgt cgcacaacc cctccgagt tcgccaggt cgaacctgg cgcacgcac acggccaga gcaagacgt ggctcaagc acctggtgc cggtcttga gctccaagc gggaggagg cgcgcaatt tggccgcct tccactttt gcatggaag cggggatca aagcacagc aggggaaca cgaatattt ttgccgaaa ttggcgcga ttgaatcgc tcaaaaaag tggcaagtt gtccgaacg ccgtggcgt cgaaatacg cagctcaag ccctgaggc tgatcggcg aggaagtgc cccacaagc tgagccaac aagtgcccc tgtggtcgg ctgaggacg tggaagagt gggtcaaac agatcgggt tcgcacaat acgccccca gttttgttg aaagtcggg tcgatggcg atcttttgc tccaactaa cggaggaga atttacgag aggatatcg ggctgacga acggaatca agcgaaagc ggtttaccc gcgagttgc aaaagttga agaaaatgg ctgattata cgtcgaggg acacggcca atattaaca cgtttttgc aaacgctgg gtccggagt ttagtattt acacgtata gtttgctga atgcgggag ttgagacca aagaagcgc tgaggaata tttccgagg atcagttgc tgaatgagt gcacaatca cgaattcga ttcaccggt ttcggatca tggaaggga tcaggcagc tggaaaatg ggcttgcga atttgaatg aggataatc tggacaagt cgttggacg tgtttgtca gttatcgaa ggtcgaacg gatctcaat tagctagtt tattaaaag tacatttac aattaaggg gattcagtg tttttatcg atgtcgagc ggctggaag cagggaaat tcgataata atctgctgc aaagtattc aaaaagcca agcatttct tgctggtgc tcacgccga atgcgctcg agaggtgcc gaggggaca ctgaacgga aagactggg tgcatagag agattgtgg cagctttgc aagctcagt gtaacatta ttcctatca ttgataatt ttcaatggc cggaagcgg acgaattac ccgaagata tgaggcaag tgtgtcact ttaacggag ttcggtgga ttcatgact atcaggacg cttgcgtgg acaaattag aaaggttca tgcgaggcg aactgagca cacgttgca ccgaaggcg tgaagtttg gtaagggcg acaacacgc cgggaaccc ccgcgaacc cggtcctgg cccgacagc cgcccagtt accagcgaa tgcacagta acgactccg gcagtggca aaggctccg acaaggacg tgaacggca acacgggac tctctaggg attgactag atggtcccc ttgcctcgg gccccccaa ctccgctgc caaggtgag tcccagggt gactgcacg acccccttg ctgcttacg actttcatt atttttgtg catatttga gccgctgat gtaactttc cctttttct attaaccgc tccccgact aacgtaaat atttcagtt ctttcttgc aggttttat aagaccgct agtccgtcg cggaactgg attatgaac acgagtcga tacccgcgg ggcttgccc ccgcagtac ccctacaac cggcgcggt tccaacagc ccgtcgggc ctcctgggg gccgggaag ccccccacc ccgaatagg tctcgcagt ctcgatggc cttctggac tcggaaccg acttgtgca gcttcagac gcaactata agcgaaccc actccgtcc caaaacacg aaaagttgt gacgattta gataaagat ttcgacgaa cagactgaa gacgttaat ttaagtgta aataacctg gacgtcgat aaagtttcg atacagagc ggttcaagt gattcgaaa cgaaaacga aatttcatg gaccgatgt gtcagtaaa gtgagatct cttattaaa aaataagtc gctggcgtg taaactata ttagtgaat tattgtatt ttcgtacac atgatgtca aagtgatat aattttatt tattattgt acctatttg ttatttata attgctgga ttccgaatc cgttttaat gtgtgaggg actgcttgc gaaagcgag cccttttta tttttttaa cggcaacta aattactta gtacaaaat tgtgctttt atatgaaat gtgatcatt atgcagcct tttaaagtc gctatgact tatttttcg acactttat tagggtaag gagtacatt aaaataatg tataaggtt tcatgttta atacgtaca gtttattgt accaagctt gcctcagta agcagtatt ggtaacgtt ctgacaatg catcattgc cttcttgtt cgaaaatat cgatgtgcg gaattaaca catttacta gtattgtaa tgttgtttt ttatgttat atttaaaaa tatatttta ttaagacac
The data is licensed under a
Creative Commons Attribution 3.0 Unported License. (CC-BY)
