Status
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Gene information
General information
TC034427
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC012667,TC012668
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 12755116..12778605
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034427
    iBeetle screen iB_05292

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    egg chamber complete not present - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection



    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (19 dpi)
    Days post injection


    Pupal morphology
    head orientation irregular - Number of animals showing the phenotype: 2

    legs partially orientation irregular - Number of animals showing the phenotype: 2

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034427 RA
      MPAQPNMNM RRVFLNRKM ATDLSVDNA NGNDHHPTM 
      MNLTIPKPR SAVTTSSQL QTNTQSSTT TTRVTSSQR 
      VHVTSSDLK ASNMKSDLI EMKNNLNDM KSSISNLNK 
      LRSSLENLA DDDDDMAEM AQPLVTFPD DTPVPSEVG 
      SPTRTVDTV KFEQKTMNN FKKTKVVKD GISAEKESA 
      KCAEMKKIQ AGDVSYEEN SAAASSRAR LEIDGISAE 
      KSQMKAKET RSLKAGDLS QQESKNMAQ TNMKVKTDK 
      FYTEQTARE SEQQRQTVT SSGVYNSEK HTSSSHSYT 
      TTTKGISTS AATTLRQIS LLNGNKEEE ILNMTCEDL 
      EQLSTTSNP QEVERAIHK YSSYLDQSI KCLQKTDQA 
      KNAPLILEK INDVMQKAW AVPTHGHEL GYSLCNALR 
      NCGGLDLLM TNCTKTDKK LQFASARLL EQCLTTENR 
      AHVVEHGLD KVVNIACAC TKNSNSVDH SRVGTGILE 
      HLFKHSEET CSDVVRLGG LDALLFECR KSDVETLRH 
      CAGALANLS LYGGAENQE AMINRKVPM WLFPLAFHH 
      DDNIKYYAC LAITVLVAN AEIEAQVLQ SGTLGLVEP 
      FVTSHNPSE FARSNLAHA HGQSKTWLK HLVPVLSSK 
      REEARNLAA FHFCMEAGI KAQQGNTNI FAEIGAIES 
      LKKVASCPN AVASKYAAQ ALRLIGEEV PHKLSQQVP 
      LWSAEDVEE WVKQIGFAQ YAPSFVESR VDGDLLLQL 
      TEENLREDI GLTNGIKRK RFTRELQKL KKMADYTSR 
      DTANINTFL QTLGPEFSI YTYSLLNAG VETKEALRN 
      ISEDQLLNE CTITNSIHR FRIMEGIRQ LENGLANLN 
      EDNLDKSLD VFVSYRRSN GSQLASLLK VHLQLRGFS 
      VFIDVERLE AGKFDNNLL QSIQKAKHF LLVLTPNAL 
      ERCRGDTER KDWVHREIV AALQAQCNI IPIIDNFQW 
      PEADELPED MRQVCHFNG VRWIHDYQD ACVDKLERF 
      MRGELSTRC TEGVKFGKG DNTPGTPAN PVLARQPPS 
      YQRMHSNDS GSGKGSDKD VNGNTGLSR D
    • >TC034427 RB
      MPAQPNMNM RRVFLNRKC YSLPAREQT RRHVNESVR 
      ILLKMYSAN RGITHSFYH ARPIGIQER IKFFKEREQ 
      MATDLSVDN ANGNDHHPT MMNLTIPKP RSAVTTSSQ 
      LQTNTQSST TTTRVTSSQ RVHVTSSDL KASNMKSDL 
      IEMKNNLND MKSSISNLN KLRSSLENL ADDDDDMAE 
      MAQPLVTFP DDTPVPSEV GSPTRTVDT VKFEQKTMN 
      NFKKTKVVK DGISAEKES AKCAEMKKI QAGDVSYEE 
      NSAAASSRA RLEIDGISA EKSQMKAKE TRSLKAGDL 
      SQQESKNMA QTNMKVKTD KFYTEQTAR ESEQQRQTV 
      TSSGVYNSE KHTSSSHSY TTTTKGIST SAATTLRQI 
      SLLNGNKEE EILNMTCED LEQLSTTSN PQEVERAIH 
      KYSSYLDQS IKCLQKTDQ AKNAPLILE KINDVMQKA 
      WAVPTHGHE LGYSLCNAL RNCGGLDLL MTNCTKTDK 
      KLQFASARL LEQCLTTEN RAHVVEHGL DKVVNIACA 
      CTKNSNSVD HSRVGTGIL EHLFKHSEE TCSDVVRLG 
      GLDALLFEC RKSDVETLR HCAGALANL SLYGGAENQ 
      EAMINRKVP MWLFPLAFH HDDNIKYYA CLAITVLVA 
      NAEIEAQVL QSGTLGLVE PFVTSHNPS EFARSNLAH 
      AHGQSKTWL KHLVPVLSS KREEARNLA AFHFCMEAG 
      IKAQQGNTN IFAEIGAIE SLKKVASCP NAVASKYAA 
      QALRLIGEE VPHKLSQQV PLWSAEDVE EWVKQIGFA 
      QYAPSFVES RVDGDLLLQ LTEENLRED IGLTNGIKR 
      KRFTRELQK LKKMADYTS RDTANINTF LQTLGPEFS 
      IYTYSLLNA GVETKEALR NISEDQLLN ECTITNSIH 
      RFRIMEGIR QLENGLANL NEDNLDKSL DVFVSYRRS 
      NGSQLASLL KVHLQLRGF SVFIDVERL EAGKFDNNL 
      LQSIQKAKH FLLVLTPNA LERCRGDTE RKDWVHREI 
      VAALQAQCN IIPIIDNFQ WPEADELPE DMRQVCHFN 
      GVRWIHDYQ DACVDKLER FMRGELSTR CTEGVKFGK 
      GDNTPGTPA NPVLARQPP SYQRMHSND SGSGKGSDK 
      DVNGNTGLS RD
    CDS sequence
    • >TC034427 RA
      atgcctgcc cagccaaat atgaacatg cgtcgggtc 
      ttcctgaac cgaaagatg gcgaccgat ttgtccgtc 
      gacaatgcc aacggaaac gatcatcat cccacgatg 
      atgaacctg acgatcccg aagccgagg tccgcagtg 
      accacatcc tcgcagttg cagacgaat acgcaaagc 
      agtacgaca acgacacgc gttacctct tcgcaacga 
      gtccatgtg acctcctcc gacctgaag gcatctaac 
      atgaaatct gatctcatc gaaatgaag aacaatctg 
      aacgacatg aagagcagc atatcgaat ctgaacaag 
      ttgcggagc tcgctggag aacctcgcc gacgacgac 
      gacgacatg gccgagatg gcgcagccc ttggtcacc 
      ttccccgac gacacgccg gtgccctcg gaggtgggc 
      tcgcccacg aggacggtc gacaccgtc aagttcgag 
      cagaagacc atgaacaac ttcaaaaag acaaaggtt 
      gtgaaagac gggataagc gccgagaag gagagtgca 
      aaatgtgcc gaaatgaag aagatccaa gccggggac 
      gtgtcctac gaggagaac agcgcagcg gcgtcttcg 
      agggcccgt ctggaaatc gacggtatc tcggcggag 
      aagagccaa atgaaagct aaggagaca cggtcactg 
      aaagctgga gatcttagc caacaagag tcgaaaaat 
      atggcacag actaatatg aaagtcaaa actgataaa 
      ttctatacg gagcaaaca gcaagggaa agtgaacaa 
      caacgacaa acggtcacc tctagtggt gtttacaat 
      tccgaaaaa cacacctca tctagtcac tcctacacg 
      accaccacc aaaggaatt agcacttcg gcagccacg 
      acgttgcga cagatcagt ttactgaac ggcaacaaa 
      gaagaagag atcctcaac atgacctgc gaggacctc 
      gagcagctc tccactacg tccaaccca caagaagtg 
      gagcgcgcc atccacaag tactcctcg tacctcgat 
      cagtcaatt aaatgcctc cagaagacc gaccaagcg 
      aaaaacgcc cccctaatc ctcgagaaa atcaacgac 
      gtgatgcag aaagcctgg gccgtgccg acccacggc 
      cacgaactg ggctattcc ctctgtaac gcgctccgc 
      aactgcggc ggcctggac ctcctcatg acaaactgc 
      accaaaacc gacaagaaa ctccaattc gcgagcgcc 
      cgcctccta gaacaatgc ctaaccacc gaaaacagg 
      gcccacgtc gtcgaacac ggcctcgac aaagtggtc 
      aacatcgcc tgcgcttgc acgaaaaac tccaactcc 
      gtggaccac tcccgcgtc ggcacgggc atcctggag 
      cacctgttc aagcacagc gaggaaacc tgcagcgac 
      gtggtcagg ctgggcggc ctggacgcc ctcctcttc 
      gaatgccgg aagagcgac gtggagact ttgcggcac 
      tgcgctggg gctttggcc aacttgagt ttatacgga 
      ggggcggag aaccaggaa gcgatgatc aatcgcaag 
      gtgccgatg tggctgttc ccgctggcc ttccaccac 
      gacgacaac atcaaatac tacgcctgt ttggcgatc 
      actgtcctg gtggcgaac gcggaaatc gaggcccaa 
      gtgctccag tcgggcacc ttggggctc gtcgagccc 
      ttcgtcacg tcgcacaac ccctccgag ttcgccagg 
      tcgaacctg gcgcacgca cacggccag agcaagacg 
      tggctcaag cacctggtg ccggtcttg agctccaag 
      cgggaggag gcgcgcaat ttggccgcc ttccacttt 
      tgcatggaa gcggggatc aaagcacag caggggaac 
      acgaatatt tttgccgaa attggcgcg attgaatcg 
      ctcaaaaaa gtggcaagt tgtccgaac gccgtggcg 
      tcgaaatac gcagctcaa gccctgagg ctgatcggc 
      gaggaagtg ccccacaag ctgagccaa caagtgccc 
      ctgtggtcg gctgaggac gtggaagag tgggtcaaa 
      cagatcggg ttcgcacaa tacgccccc agttttgtt 
      gaaagtcgg gtcgatggc gatcttttg ctccaacta 
      acggaggag aatttacga gaggatatc gggctgacg 
      aacggaatc aagcgaaag cggtttacc cgcgagttg 
      caaaagttg aagaaaatg gctgattat acgtcgagg 
      gacacggcc aatattaac acgtttttg caaacgctg 
      ggtccggag tttagtatt tacacgtat agtttgctg 
      aatgcggga gttgagacc aaagaagcg ctgaggaat 
      atttccgag gatcagttg ctgaatgag tgcacaatc 
      acgaattcg attcaccgg tttcggatc atggaaggg 
      atcaggcag ctggaaaat gggcttgcg aatttgaat 
      gaggataat ctggacaag tcgttggac gtgtttgtc 
      agttatcga aggtcgaac ggatctcaa ttagctagt 
      ttattaaaa gtacattta caattaagg ggattcagt 
      gtttttatc gatgtcgag cggctggaa gcagggaaa 
      ttcgataat aatctgctg caaagtatt caaaaagcc 
      aagcatttc ttgctggtg ctcacgccg aatgcgctc 
      gagaggtgc cgaggggac actgaacgg aaagactgg 
      gtgcataga gagattgtg gcagctttg caagctcag 
      tgtaacatt attcctatc attgataat tttcaatgg 
      ccggaagcg gacgaatta cccgaagat atgaggcaa 
      gtgtgtcac tttaacgga gttcggtgg attcatgac 
      tatcaggac gcttgcgtg gacaaatta gaaaggttc 
      atgcgaggc gaactgagc acacgttgc accgaaggc 
      gtgaagttt ggtaagggc gacaacacg ccgggaacc 
      cccgcgaac ccggtcctg gcccgacag ccgcccagt 
      taccagcga atgcacagt aacgactcc ggcagtggc 
      aaaggctcc gacaaggac gtgaacggc aacacggga 
      ctctctagg gattga
    • >TC034427 RB
      atgcctgcc cagccaaat atgaacatg cgtcgggtc 
      ttcctgaac cgaaagtgc tattcctta cctgcgagg 
      gagcagacg cgtcgccat gtgaatgaa agtgttcga 
      attttgctc aaaatgtac tcagccaat cggggaatc 
      acgcacagc ttctaccac gcgcggccg atcggcatc 
      caagaacgg attaaattt ttcaaagag cgggaacag 
      atggcgacc gatttgtcc gtcgacaat gccaacgga 
      aacgatcat catcccacg atgatgaac ctgacgatc 
      ccgaagccg aggtccgca gtgaccaca tcctcgcag 
      ttgcagacg aatacgcaa agcagtacg acaacgaca 
      cgcgttacc tcttcgcaa cgagtccat gtgacctcc 
      tccgacctg aaggcatct aacatgaaa tctgatctc 
      atcgaaatg aagaacaat ctgaacgac atgaagagc 
      agcatatcg aatctgaac aagttgcgg agctcgctg 
      gagaacctc gccgacgac gacgacgac atggccgag 
      atggcgcag cccttggtc accttcccc gacgacacg 
      ccggtgccc tcggaggtg ggctcgccc acgaggacg 
      gtcgacacc gtcaagttc gagcagaag accatgaac 
      aacttcaaa aagacaaag gttgtgaaa gacgggata 
      agcgccgag aaggagagt gcaaaatgt gccgaaatg 
      aagaagatc caagccggg gacgtgtcc tacgaggag 
      aacagcgca gcggcgtct tcgagggcc cgtctggaa 
      atcgacggt atctcggcg gagaagagc caaatgaaa 
      gctaaggag acacggtca ctgaaagct ggagatctt 
      agccaacaa gagtcgaaa aatatggca cagactaat 
      atgaaagtc aaaactgat aaattctat acggagcaa 
      acagcaagg gaaagtgaa caacaacga caaacggtc 
      acctctagt ggtgtttac aattccgaa aaacacacc 
      tcatctagt cactcctac acgaccacc accaaagga 
      attagcact tcggcagcc acgacgttg cgacagatc 
      agtttactg aacggcaac aaagaagaa gagatcctc 
      aacatgacc tgcgaggac ctcgagcag ctctccact 
      acgtccaac ccacaagaa gtggagcgc gccatccac 
      aagtactcc tcgtacctc gatcagtca attaaatgc 
      ctccagaag accgaccaa gcgaaaaac gccccccta 
      atcctcgag aaaatcaac gacgtgatg cagaaagcc 
      tgggccgtg ccgacccac ggccacgaa ctgggctat 
      tccctctgt aacgcgctc cgcaactgc ggcggcctg 
      gacctcctc atgacaaac tgcaccaaa accgacaag 
      aaactccaa ttcgcgagc gcccgcctc ctagaacaa 
      tgcctaacc accgaaaac agggcccac gtcgtcgaa 
      cacggcctc gacaaagtg gtcaacatc gcctgcgct 
      tgcacgaaa aactccaac tccgtggac cactcccgc 
      gtcggcacg ggcatcctg gagcacctg ttcaagcac 
      agcgaggaa acctgcagc gacgtggtc aggctgggc 
      ggcctggac gccctcctc ttcgaatgc cggaagagc 
      gacgtggag actttgcgg cactgcgct ggggctttg 
      gccaacttg agtttatac ggaggggcg gagaaccag 
      gaagcgatg atcaatcgc aaggtgccg atgtggctg 
      ttcccgctg gccttccac cacgacgac aacatcaaa 
      tactacgcc tgtttggcg atcactgtc ctggtggcg 
      aacgcggaa atcgaggcc caagtgctc cagtcgggc 
      accttgggg ctcgtcgag cccttcgtc acgtcgcac 
      aacccctcc gagttcgcc aggtcgaac ctggcgcac 
      gcacacggc cagagcaag acgtggctc aagcacctg 
      gtgccggtc ttgagctcc aagcgggag gaggcgcgc 
      aatttggcc gccttccac ttttgcatg gaagcgggg 
      atcaaagca cagcagggg aacacgaat atttttgcc 
      gaaattggc gcgattgaa tcgctcaaa aaagtggca 
      agttgtccg aacgccgtg gcgtcgaaa tacgcagct 
      caagccctg aggctgatc ggcgaggaa gtgccccac 
      aagctgagc caacaagtg cccctgtgg tcggctgag 
      gacgtggaa gagtgggtc aaacagatc gggttcgca 
      caatacgcc cccagtttt gttgaaagt cgggtcgat 
      ggcgatctt ttgctccaa ctaacggag gagaattta 
      cgagaggat atcgggctg acgaacgga atcaagcga 
      aagcggttt acccgcgag ttgcaaaag ttgaagaaa 
      atggctgat tatacgtcg agggacacg gccaatatt 
      aacacgttt ttgcaaacg ctgggtccg gagtttagt 
      atttacacg tatagtttg ctgaatgcg ggagttgag 
      accaaagaa gcgctgagg aatatttcc gaggatcag 
      ttgctgaat gagtgcaca atcacgaat tcgattcac 
      cggtttcgg atcatggaa gggatcagg cagctggaa 
      aatgggctt gcgaatttg aatgaggat aatctggac 
      aagtcgttg gacgtgttt gtcagttat cgaaggtcg 
      aacggatct caattagct agtttatta aaagtacat 
      ttacaatta aggggattc agtgttttt atcgatgtc 
      gagcggctg gaagcaggg aaattcgat aataatctg 
      ctgcaaagt attcaaaaa gccaagcat ttcttgctg 
      gtgctcacg ccgaatgcg ctcgagagg tgccgaggg 
      gacactgaa cggaaagac tgggtgcat agagagatt 
      gtggcagct ttgcaagct cagtgtaac attattcct 
      atcattgat aattttcaa tggccggaa gcggacgaa 
      ttacccgaa gatatgagg caagtgtgt cactttaac 
      ggagttcgg tggattcat gactatcag gacgcttgc 
      gtggacaaa ttagaaagg ttcatgcga ggcgaactg 
      agcacacgt tgcaccgaa ggcgtgaag tttggtaag 
      ggcgacaac acgccggga acccccgcg aacccggtc 
      ctggcccga cagccgccc agttaccag cgaatgcac 
      agtaacgac tccggcagt ggcaaaggc tccgacaag 
      gacgtgaac ggcaacacg ggactctct agggattga
    mRNA sequence
    • >TC034427 RA
      gagaatgaa aaaatgctt ggcaaatct tcaaagctt 
      tttatttta gttttatcg ttttattgg cgaaaataa 
      cgtcatttt acggatgtt catgtgaaa gtattttta 
      agttgttct tttgcggcc gcaccagat gtcgcctca 
      tctcgaaac tgaatagtt ttcgtgtta ttgagaacg 
      tgtgctcct cgctactaa aattgtaaa caaaatgac 
      ctaaaagca ccatcaaaa tccataact aaagaataa 
      tttatgata ataaaaaat ctaacacac tctcgtctc 
      atttttccc attcttcca tagctctaa cattatttt 
      tcttggcct tgcttggat tatttgcct tttctactt 
      gggttattt ataagtttc ggtgtggag gaaaaaact 
      aatcaagtg gacggccaa ttacataat aacacacct 
      aacagtgca catctgcta ctaaaccat taccataag 
      taaaaagaa tggacggga aaattgtcc ccgaaagac 
      acaagcgga aacaaaaga atcccgatt tgaaactta 
      agtaagcaa cggttgcgc gttcgagtc gcttgtgaa 
      gtcactgaa atttcagca acttctgct atgtgtttt 
      tgtgtcaaa taggccggt gtggtgata ttgtgtcaa 
      gcatggttg tgaataaaa ttacaatgc ctgcccagc 
      caaatatga acatgcgtc gggtcttcc tgaaccgaa 
      agatggcga ccgatttgt ccgtcgaca atgccaacg 
      gaaacgatc atcatccca cgatgatga acctgacga 
      tcccgaagc cgaggtccg cagtgacca catcctcgc 
      agttgcaga cgaatacgc aaagcagta cgacaacga 
      cacgcgtta cctcttcgc aacgagtcc atgtgacct 
      cctccgacc tgaaggcat ctaacatga aatctgatc 
      tcatcgaaa tgaagaaca atctgaacg acatgaaga 
      gcagcatat cgaatctga acaagttgc ggagctcgc 
      tggagaacc tcgccgacg acgacgacg acatggccg 
      agatggcgc agcccttgg tcaccttcc ccgacgaca 
      cgccggtgc cctcggagg tgggctcgc ccacgagga 
      cggtcgaca ccgtcaagt tcgagcaga agaccatga 
      acaacttca aaaagacaa aggttgtga aagacggga 
      taagcgccg agaaggaga gtgcaaaat gtgccgaaa 
      tgaagaaga tccaagccg gggacgtgt cctacgagg 
      agaacagcg cagcggcgt cttcgaggg cccgtctgg 
      aaatcgacg gtatctcgg cggagaaga gccaaatga 
      aagctaagg agacacggt cactgaaag ctggagatc 
      ttagccaac aagagtcga aaaatatgg cacagacta 
      atatgaaag tcaaaactg ataaattct atacggagc 
      aaacagcaa gggaaagtg aacaacaac gacaaacgg 
      tcacctcta gtggtgttt acaattccg aaaaacaca 
      cctcatcta gtcactcct acacgacca ccaccaaag 
      gaattagca cttcggcag ccacgacgt tgcgacaga 
      tcagtttac tgaacggca acaaagaag aagagatcc 
      tcaacatga cctgcgagg acctcgagc agctctcca 
      ctacgtcca acccacaag aagtggagc gcgccatcc 
      acaagtact cctcgtacc tcgatcagt caattaaat 
      gcctccaga agaccgacc aagcgaaaa acgcccccc 
      taatcctcg agaaaatca acgacgtga tgcagaaag 
      cctgggccg tgccgaccc acggccacg aactgggct 
      attccctct gtaacgcgc tccgcaact gcggcggcc 
      tggacctcc tcatgacaa actgcacca aaaccgaca 
      agaaactcc aattcgcga gcgcccgcc tcctagaac 
      aatgcctaa ccaccgaaa acagggccc acgtcgtcg 
      aacacggcc tcgacaaag tggtcaaca tcgcctgcg 
      cttgcacga aaaactcca actccgtgg accactccc 
      gcgtcggca cgggcatcc tggagcacc tgttcaagc 
      acagcgagg aaacctgca gcgacgtgg tcaggctgg 
      gcggcctgg acgccctcc tcttcgaat gccggaaga 
      gcgacgtgg agactttgc ggcactgcg ctggggctt 
      tggccaact tgagtttat acggagggg cggagaacc 
      aggaagcga tgatcaatc gcaaggtgc cgatgtggc 
      tgttcccgc tggccttcc accacgacg acaacatca 
      aatactacg cctgtttgg cgatcactg tcctggtgg 
      cgaacgcgg aaatcgagg cccaagtgc tccagtcgg 
      gcaccttgg ggctcgtcg agcccttcg tcacgtcgc 
      acaacccct ccgagttcg ccaggtcga acctggcgc 
      acgcacacg gccagagca agacgtggc tcaagcacc 
      tggtgccgg tcttgagct ccaagcggg aggaggcgc 
      gcaatttgg ccgccttcc acttttgca tggaagcgg 
      ggatcaaag cacagcagg ggaacacga atatttttg 
      ccgaaattg gcgcgattg aatcgctca aaaaagtgg 
      caagttgtc cgaacgccg tggcgtcga aatacgcag 
      ctcaagccc tgaggctga tcggcgagg aagtgcccc 
      acaagctga gccaacaag tgcccctgt ggtcggctg 
      aggacgtgg aagagtggg tcaaacaga tcgggttcg 
      cacaatacg cccccagtt ttgttgaaa gtcgggtcg 
      atggcgatc ttttgctcc aactaacgg aggagaatt 
      tacgagagg atatcgggc tgacgaacg gaatcaagc 
      gaaagcggt ttacccgcg agttgcaaa agttgaaga 
      aaatggctg attatacgt cgagggaca cggccaata 
      ttaacacgt ttttgcaaa cgctgggtc cggagttta 
      gtatttaca cgtatagtt tgctgaatg cgggagttg 
      agaccaaag aagcgctga ggaatattt ccgaggatc 
      agttgctga atgagtgca caatcacga attcgattc 
      accggtttc ggatcatgg aagggatca ggcagctgg 
      aaaatgggc ttgcgaatt tgaatgagg ataatctgg 
      acaagtcgt tggacgtgt ttgtcagtt atcgaaggt 
      cgaacggat ctcaattag ctagtttat taaaagtac 
      atttacaat taaggggat tcagtgttt ttatcgatg 
      tcgagcggc tggaagcag ggaaattcg ataataatc 
      tgctgcaaa gtattcaaa aagccaagc atttcttgc 
      tggtgctca cgccgaatg cgctcgaga ggtgccgag 
      gggacactg aacggaaag actgggtgc atagagaga 
      ttgtggcag ctttgcaag ctcagtgta acattattc 
      ctatcattg ataattttc aatggccgg aagcggacg 
      aattacccg aagatatga ggcaagtgt gtcacttta 
      acggagttc ggtggattc atgactatc aggacgctt 
      gcgtggaca aattagaaa ggttcatgc gaggcgaac 
      tgagcacac gttgcaccg aaggcgtga agtttggta 
      agggcgaca acacgccgg gaacccccg cgaacccgg 
      tcctggccc gacagccgc ccagttacc agcgaatgc 
      acagtaacg actccggca gtggcaaag gctccgaca 
      aggacgtga acggcaaca cgggactct ctagggatt 
      gactagatg gtccccttg cctcgggcc ccccaactc 
      cgctgccaa ggtgagtcc cagggtgac tgcacgacc 
      cccttgctg cttacgact ttcattatt tttgtgcat 
      atttgagcc gctgatgta actttccct ttttctatt 
      aaccgctcc ccgactaac gtaaatatt tcagttctt 
      tcttgcagg ttttataag accgctagt ccgtcgcgg 
      aactggatt atgaacacg agtcgatac ccgcggggc 
      ttgcccccg cagtacccc tacaaccgg cgcggttcc 
      aacagcccg tcgggcctc ctgggggcc gggaagccc 
      cccaccccg aataggtct cgcagtctc gatggcctt 
      ctggactcg gaaccgact tgtgcagct tcagacgca 
      actataagc gaacccact ccgtcccaa aacacgaaa 
      agttgtgac gatttagat aaagatttc gacgaacag 
      actgaagac gttaattta agtgtaaat aacctggac 
      gtcgataaa gtttcgata cagagcggt tcaagtgat 
      tcgaaacga aaacgaaat ttcatggac cgatgtgtc 
      agtaaagtg agatctctt attaaaaaa taagtcgct 
      ggcgtgtaa actatatta gtgaattat tgtattttc 
      gtacacatg atgtcaaag tgatataat tttatttat 
      tattgtacc tatttgtta tttataatt gctggattc 
      cgaatccgt tttaatgtg tgagggact gcttgcgaa 
      agcgagccc tttttattt ttttaacgg caactaaat 
      tacttagta caaaattgt gcttttata tgaaatgtg 
      atcattatg cagcctttt aaagtcgct atgacttat 
      ttttcgaca ctttattag ggtaaggag tacattaaa 
      ataatgtat aaggtttca tgtttaata cgtacagtt 
      tattgtacc aagcttgcc tcagtaagc agtattggt 
      aacgttctg acaatgcat cattgcctt cttgttcga 
      aaatatcga tgtgcggaa ttaacacat ttactagta 
      ttgtaatgt tgtttttta tgttatatt taaaaatat 
      attttatta agacac
    • >TC034427 RB
      gagaatgaa aaaatgctt ggcaaatct tcaaagctt 
      tttatttta gttttatcg ttttattgg cgaaaataa 
      cgtcatttt acggatgtt catgtgaaa gtattttta 
      agttgttct tttgcggcc gcaccagat gtcgcctca 
      tctcgaaac tgaatagtt ttcgtgtta ttgagaacg 
      tgtgctcct cgctactaa aattgtaaa caaaatgac 
      ctaaaagca ccatcaaaa tccataact aaagaataa 
      tttatgata ataaaaaat ctaacacac tctcgtctc 
      atttttccc attcttcca tagctctaa cattatttt 
      tcttggcct tgcttggat tatttgcct tttctactt 
      gggttattt ataagtttc ggtgtggag gaaaaaact 
      aatcaagtg gacggccaa ttacataat aacacacct 
      aacagtgca catctgcta ctaaaccat taccataag 
      taaaaagaa tggacggga aaattgtcc ccgaaagac 
      acaagcgga aacaaaaga atcccgatt tgaaactta 
      agtaagcaa cggttgcgc gttcgagtc gcttgtgaa 
      gtcactgaa atttcagca acttctgct atgtgtttt 
      tgtgtcaaa taggccggt gtggtgata ttgtgtcaa 
      gcatggttg tgaataaaa ttacaatgc ctgcccagc 
      caaatatga acatgcgtc gggtcttcc tgaaccgaa 
      agtgctatt ccttacctg cgagggagc agacgcgtc 
      gccatgtga atgaaagtg ttcgaattt tgctcaaaa 
      tgtactcag ccaatcggg gaatcacgc acagcttct 
      accacgcgc ggccgatcg gcatccaag aacggatta 
      aatttttca aagagcggg aacagatgg cgaccgatt 
      tgtccgtcg acaatgcca acggaaacg atcatcatc 
      ccacgatga tgaacctga cgatcccga agccgaggt 
      ccgcagtga ccacatcct cgcagttgc agacgaata 
      cgcaaagca gtacgacaa cgacacgcg ttacctctt 
      cgcaacgag tccatgtga cctcctccg acctgaagg 
      catctaaca tgaaatctg atctcatcg aaatgaaga 
      acaatctga acgacatga agagcagca tatcgaatc 
      tgaacaagt tgcggagct cgctggaga acctcgccg 
      acgacgacg acgacatgg ccgagatgg cgcagccct 
      tggtcacct tccccgacg acacgccgg tgccctcgg 
      aggtgggct cgcccacga ggacggtcg acaccgtca 
      agttcgagc agaagacca tgaacaact tcaaaaaga 
      caaaggttg tgaaagacg ggataagcg ccgagaagg 
      agagtgcaa aatgtgccg aaatgaaga agatccaag 
      ccggggacg tgtcctacg aggagaaca gcgcagcgg 
      cgtcttcga gggcccgtc tggaaatcg acggtatct 
      cggcggaga agagccaaa tgaaagcta aggagacac 
      ggtcactga aagctggag atcttagcc aacaagagt 
      cgaaaaata tggcacaga ctaatatga aagtcaaaa 
      ctgataaat tctatacgg agcaaacag caagggaaa 
      gtgaacaac aacgacaaa cggtcacct ctagtggtg 
      tttacaatt ccgaaaaac acacctcat ctagtcact 
      cctacacga ccaccacca aaggaatta gcacttcgg 
      cagccacga cgttgcgac agatcagtt tactgaacg 
      gcaacaaag aagaagaga tcctcaaca tgacctgcg 
      aggacctcg agcagctct ccactacgt ccaacccac 
      aagaagtgg agcgcgcca tccacaagt actcctcgt 
      acctcgatc agtcaatta aatgcctcc agaagaccg 
      accaagcga aaaacgccc ccctaatcc tcgagaaaa 
      tcaacgacg tgatgcaga aagcctggg ccgtgccga 
      cccacggcc acgaactgg gctattccc tctgtaacg 
      cgctccgca actgcggcg gcctggacc tcctcatga 
      caaactgca ccaaaaccg acaagaaac tccaattcg 
      cgagcgccc gcctcctag aacaatgcc taaccaccg 
      aaaacaggg cccacgtcg tcgaacacg gcctcgaca 
      aagtggtca acatcgcct gcgcttgca cgaaaaact 
      ccaactccg tggaccact cccgcgtcg gcacgggca 
      tcctggagc acctgttca agcacagcg aggaaacct 
      gcagcgacg tggtcaggc tgggcggcc tggacgccc 
      tcctcttcg aatgccgga agagcgacg tggagactt 
      tgcggcact gcgctgggg ctttggcca acttgagtt 
      tatacggag gggcggaga accaggaag cgatgatca 
      atcgcaagg tgccgatgt ggctgttcc cgctggcct 
      tccaccacg acgacaaca tcaaatact acgcctgtt 
      tggcgatca ctgtcctgg tggcgaacg cggaaatcg 
      aggcccaag tgctccagt cgggcacct tggggctcg 
      tcgagccct tcgtcacgt cgcacaacc cctccgagt 
      tcgccaggt cgaacctgg cgcacgcac acggccaga 
      gcaagacgt ggctcaagc acctggtgc cggtcttga 
      gctccaagc gggaggagg cgcgcaatt tggccgcct 
      tccactttt gcatggaag cggggatca aagcacagc 
      aggggaaca cgaatattt ttgccgaaa ttggcgcga 
      ttgaatcgc tcaaaaaag tggcaagtt gtccgaacg 
      ccgtggcgt cgaaatacg cagctcaag ccctgaggc 
      tgatcggcg aggaagtgc cccacaagc tgagccaac 
      aagtgcccc tgtggtcgg ctgaggacg tggaagagt 
      gggtcaaac agatcgggt tcgcacaat acgccccca 
      gttttgttg aaagtcggg tcgatggcg atcttttgc 
      tccaactaa cggaggaga atttacgag aggatatcg 
      ggctgacga acggaatca agcgaaagc ggtttaccc 
      gcgagttgc aaaagttga agaaaatgg ctgattata 
      cgtcgaggg acacggcca atattaaca cgtttttgc 
      aaacgctgg gtccggagt ttagtattt acacgtata 
      gtttgctga atgcgggag ttgagacca aagaagcgc 
      tgaggaata tttccgagg atcagttgc tgaatgagt 
      gcacaatca cgaattcga ttcaccggt ttcggatca 
      tggaaggga tcaggcagc tggaaaatg ggcttgcga 
      atttgaatg aggataatc tggacaagt cgttggacg 
      tgtttgtca gttatcgaa ggtcgaacg gatctcaat 
      tagctagtt tattaaaag tacatttac aattaaggg 
      gattcagtg tttttatcg atgtcgagc ggctggaag 
      cagggaaat tcgataata atctgctgc aaagtattc 
      aaaaagcca agcatttct tgctggtgc tcacgccga 
      atgcgctcg agaggtgcc gaggggaca ctgaacgga 
      aagactggg tgcatagag agattgtgg cagctttgc 
      aagctcagt gtaacatta ttcctatca ttgataatt 
      ttcaatggc cggaagcgg acgaattac ccgaagata 
      tgaggcaag tgtgtcact ttaacggag ttcggtgga 
      ttcatgact atcaggacg cttgcgtgg acaaattag 
      aaaggttca tgcgaggcg aactgagca cacgttgca 
      ccgaaggcg tgaagtttg gtaagggcg acaacacgc 
      cgggaaccc ccgcgaacc cggtcctgg cccgacagc 
      cgcccagtt accagcgaa tgcacagta acgactccg 
      gcagtggca aaggctccg acaaggacg tgaacggca 
      acacgggac tctctaggg attgactag atggtcccc 
      ttgcctcgg gccccccaa ctccgctgc caaggtgag 
      tcccagggt gactgcacg acccccttg ctgcttacg 
      actttcatt atttttgtg catatttga gccgctgat 
      gtaactttc cctttttct attaaccgc tccccgact 
      aacgtaaat atttcagtt ctttcttgc aggttttat 
      aagaccgct agtccgtcg cggaactgg attatgaac 
      acgagtcga tacccgcgg ggcttgccc ccgcagtac 
      ccctacaac cggcgcggt tccaacagc ccgtcgggc 
      ctcctgggg gccgggaag ccccccacc ccgaatagg 
      tctcgcagt ctcgatggc cttctggac tcggaaccg 
      acttgtgca gcttcagac gcaactata agcgaaccc 
      actccgtcc caaaacacg aaaagttgt gacgattta 
      gataaagat ttcgacgaa cagactgaa gacgttaat 
      ttaagtgta aataacctg gacgtcgat aaagtttcg 
      atacagagc ggttcaagt gattcgaaa cgaaaacga 
      aatttcatg gaccgatgt gtcagtaaa gtgagatct 
      cttattaaa aaataagtc gctggcgtg taaactata 
      ttagtgaat tattgtatt ttcgtacac atgatgtca 
      aagtgatat aattttatt tattattgt acctatttg 
      ttatttata attgctgga ttccgaatc cgttttaat 
      gtgtgaggg actgcttgc gaaagcgag cccttttta 
      tttttttaa cggcaacta aattactta gtacaaaat 
      tgtgctttt atatgaaat gtgatcatt atgcagcct 
      tttaaagtc gctatgact tatttttcg acactttat 
      tagggtaag gagtacatt aaaataatg tataaggtt 
      tcatgttta atacgtaca gtttattgt accaagctt 
      gcctcagta agcagtatt ggtaacgtt ctgacaatg 
      catcattgc cttcttgtt cgaaaatat cgatgtgcg 
      gaattaaca catttacta gtattgtaa tgttgtttt 
      ttatgttat atttaaaaa tatatttta ttaagacac
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