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Gene information
General information
TC012666 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 12717456..12751810
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012666
    iBeetle screen iB_05291

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    legs shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012666 RA
      MAVVGPSRH SRVSMSVSM SLMQGTAGP TAGGQQGGL 
      HAAQPYYHP QHAAAPAHT QEQVQPDRP IGYGAFGVV 
      WAVTDPRDG RRVALKKLP NVFASLVSS KRVFRELKM 
      LCFFKHENV LSALDILQP PHLDFFQEI YVITELLQS 
      DLHKIIVSH QPLSSDHIK VFLYQILRG LKYLHSARI 
      LHRDIKPGN LLVNSNCVL KICDFGLAR VEEPDSSKH 
      MTQEVVTQY YRAPEILMG AKHYTAAVD VWSVGCIFG 
      ELLGRRILF QAQNPVQQL ELITELLGT PSLDDMKYA 
      CEGAKTHML RRAPKPPSL SAFYTLSSQ ATHEVVHLL 
      CQMLVFDPD KRISVVDAL AHPYLDEGR LRYHSCMCK 
      CCYTTASGM RQYTSEFET TAPQGFDDH WEKKLTSVQ 
      HVKEEMHKF IAEQLNTSR VPLCINPQS AAFKSFASS 
      TVAHPSELP PSPHQWD
    CDS sequence
    • >TC012666 RA
      atggctgtg gtgggcccc agccgccac tcaagggtg 
      tccatgtcc gtgtccatg agtctgatg cagggtacc 
      gccggcccc accgccggg gggcagcaa gggggcctg 
      cacgcggcg cagccctac taccacccc cagcatgca 
      gcggccccg gcgcacacc caggagcag gtccagccg 
      gaccgaccg atcggatac ggagctttc ggagtcgtt 
      tgggcggtg acggatccc cgcgacggc aggagggtc 
      gccctgaag aagttaccg aatgtgttc gcatcgtta 
      gtttcatcg aaaagagtg tttagagaa ctgaaaatg 
      ctctgcttt ttcaaacac gaaaacgtt ctttccgcc 
      ttagatatc ctccaaccg cctcactta gacttcttc 
      caggaaata tatgtaata acggaattg cttcaaagc 
      gatttacac aaaattata gtatcacac cagcctctt 
      tcctcggat cacatcaag gtgtttctc taccagata 
      cttagaggt ttaaaatat ttacattcg gctaggata 
      ttacataga gacataaaa cctggaaac cttttagta 
      aacagcaat tgtgttctt aagatctgt gattttggt 
      ttagcgaga gtagaagaa cccgactcg tccaaacat 
      atgacccaa gaagtggta acgcagtat tacagagct 
      ccggaaatt ctaatggga gcgaaacat tacacggcg 
      gctgtggac gtgtggtct gtcgggtgc attttcggg 
      gaattactg ggccggagg atactgttc caggcccaa 
      aatcccgta caacaatta gaattaata acggaattg 
      ttaggaacg ccgtcgttg gacgatatg aaatacgcc 
      tgcgaaggc gccaagacg catatgctg aggagggcg 
      cccaagccg ccttcgtta tcggctttt tacacgctg 
      agttcacag gcgacgcac gaagtggtc catctgctc 
      tgtcagatg ctcgttttt gacccggac aaacgtatc 
      tcggtagtg gacgccttg gcgcacccc tatttggac 
      gagggccgt ctgcggtac cactcgtgc atgtgcaaa 
      tgctgctac acgacagcc agcggcatg agacaatac 
      acgtccgaa tttgaaacg accgccccc caaggattc 
      gacgatcat tgggaaaag aagctgacg tctgtacaa 
      catgtgaaa gaggagatg cataagttc atagcagag 
      caactgaat actagtaga gttcctctt tgcataaac 
      ccccaatct gcggcgttc aagagtttc gccagttcg 
      actgtagct cacccatcc gagttgcct ccttcgccc 
      caccaatgg gattga
    mRNA sequence
    • >TC012666 RA
      gagtatcag aaatggtgg ggtcgcaaa tgtttggaa 
      ctcctgcag ccatgttta gtagaattt cagttcgca 
      tcgaacggt tgtgaaagt tgtgcattg tgtgtcaca 
      aatttacaa cttatttcc ttattttta tttccccct 
      tgttttgca ccaaatcct cgaaagtag ctttccgtt 
      gcgcgttcg tttccgttg aagaaaaag ggcaaaatt 
      aaagtgtgg agcagtgcg ttaacatga cggtgaaat 
      taaatttaa ttacaagtt ctctttaca aggactcga 
      aattagcat tttaccacc aagttcact cgtaattcg 
      tccagtgac tcgtaatga aacaaacta gtgtcctag 
      tgtaggaaa ggaaagtcg ccgttttcg cataagccg 
      accgcaagg ataacgagt agtcaaaag gctttgtta 
      aaaaacccg tcgaccgag ccgagtcct ggtgcggtg 
      gtggtgact atcacggga gggaaaaaa cagcgtcga 
      actggtgca gtatttgat ggctgtggt gggccccag 
      ccgccactc aagggtgtc catgtccgt gtccatgag 
      tctgatgca gggtaccgc cggccccac cgccggggg 
      gcagcaagg gggcctgca cgcggcgca gccctacta 
      ccaccccca gcatgcagc ggccccggc gcacaccca 
      ggagcaggt ccagccgga ccgaccgat cggatacgg 
      agctttcgg agtcgtttg ggcggtgac ggatccccg 
      cgacggcag gagggtcgc cctgaagaa gttaccgaa 
      tgtgttcgc atcgttagt ttcatcgaa aagagtgtt 
      tagagaact gaaaatgct ctgcttttt caaacacga 
      aaacgttct ttccgcctt agatatcct ccaaccgcc 
      tcacttaga cttcttcca ggaaatata tgtaataac 
      ggaattgct tcaaagcga tttacacaa aattatagt 
      atcacacca gcctctttc ctcggatca catcaaggt 
      gtttctcta ccagatact tagaggttt aaaatattt 
      acattcggc taggatatt acatagaga cataaaacc 
      tggaaacct tttagtaaa cagcaattg tgttcttaa 
      gatctgtga ttttggttt agcgagagt agaagaacc 
      cgactcgtc caaacatat gacccaaga agtggtaac 
      gcagtatta cagagctcc ggaaattct aatgggagc 
      gaaacatta cacggcggc tgtggacgt gtggtctgt 
      cgggtgcat tttcgggga attactggg ccggaggat 
      actgttcca ggcccaaaa tcccgtaca acaattaga 
      attaataac ggaattgtt aggaacgcc gtcgttgga 
      cgatatgaa atacgcctg cgaaggcgc caagacgca 
      tatgctgag gagggcgcc caagccgcc ttcgttatc 
      ggcttttta cacgctgag ttcacaggc gacgcacga 
      agtggtcca tctgctctg tcagatgct cgtttttga 
      cccggacaa acgtatctc ggtagtgga cgccttggc 
      gcaccccta tttggacga gggccgtct gcggtacca 
      ctcgtgcat gtgcaaatg ctgctacac gacagccag 
      cggcatgag acaatacac gtccgaatt tgaaacgac 
      cgcccccca aggattcga cgatcattg ggaaaagaa 
      gctgacgtc tgtacaaca tgtgaaaga ggagatgca 
      taagttcat agcagagca actgaatac tagtagagt 
      tcctctttg cataaaccc ccaatctgc ggcgttcaa 
      gagtttcgc cagttcgac tgtagctca cccatccga 
      gttgcctcc ttcgcccca ccaatggga ttgaccatg 
      gggtgttgt ccgtagata accactctc ttctcgtct 
      ctttccgtt gttcgatga acaataatt atttataag 
      taacagtac accaaatat attcattca gggattcta 
      tgcaatgca aattatgga caatggaca atcgcgctg 
      caacttatc gtagtgtgt gtttcgtgt atatttagt 
      cgctcagag tgtgatttt atctatacc atgtagatc 
      agccaattt agatacagt gtacagata tcactgcct 
      tgattagta taatagaat ttttagttg aataagact 
      ggcaggttt tttatgttg ggcggttga tcacatgtc 
      ggtattcca accagaatc aaaattttt cactcagct 
      ccaccactc ccgacatcg atttaactg ccttcgaca 
      agcaccaag tttaccgca aatttggta gtttaacgt 
      taatttaag acttgtaag gtaaagact gtgaatata 
      gctacatat aaacccaga aacgatgtg cagctctat 
      aaattcctt tatagcgaa cttcatgtt ataattgcc 
      aactttaca tataaatag tatgtactg tagtgtatt 
      cagtgtgag ttgttaagt gtgcaatga gattagaga 
      ctgggaaaa ctcggctcg agttcagct ctcctctgt 
      tcattagtt gacattttt ttactaagc cgatgtagt 
      gtaatggaa gtgaattgg ttatacagt ggcctttac 
      tttgtatta gctcatttg gaactctga tttgacgct 
      caatgtgct attttacga ttggttcag ttggtagca 
      ccgcaagcc tcctacagg gaaattata ggcttttat 
      cgtgttttt gtaaatatt atattttta ttgtgagag 
      gaaattgac actttaggt ttagcataa gtttagtaa 
      attttatta agtgtaatt tttctgtag gaaatttta 
      gtaaccttg ttttaacga aatttgtag ctactttaa 
      ttctttgta aataacaca acagtacac ttaagtcac 
      tatccttct ttcgactgc tattttgca aatgattac 
      gaccaaaca acaaattat ttgcaaagt tggtgattt 
      gataaaatg ttgtaaata gtcatgttt actctttcg 
      taggtttag ttaattgta ttctaattc tagcagaaa 
      ttgacgaaa aaaattcag aaatatttt catgtcaat 
      aaccacaac atgtaaata ttgtccggc gcttttcga 
      gttccatta gcattattt aacgaattt gtgtaatta 
      ttccaatgg tttttccag agatctcat acgcgtatg 
      taaatatat tttataaag gcacctctc ttgctcaat 
      attattgcg caataacta ttacgcaat actgtaact 
      acttatttt atcagttcg ccctttaac gttcaatat 
      tatgcaaaa ttctattgc atgacaata ttgggcgtg 
      tagggctgc ctttattca gactttttt cgagtaatg 
      gtcagtcta tgtttactt taacttagt atactctga 
      tatgaaaaa ataaacaac acttatgta c
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