Status
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Gene information
General information
TC012662 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 12671136..12674116
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012662
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05289

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012662 RA
      MLSFYLLLT GVGLILLLQ TWRFFRKYF KNKTILKKI 
      RGPPETPIF GHMYIFTQD PDLLFSRIR KYSREYYPI 
      FRFSALYVD IVNFLEPND IELILSTTK HLSKSKIYT 
      FLNNWLGTG LLTSTGAKW HQRRKILTP AFHFNILQQ 
      FLNIFNDET EKLVLRLGE DCGRTIDVV PPVTNFTLQ 
      SIAETAMGL SNINELTQK EYKKAIYKI GHIFLKRLS 
      KPWYRLDAI YNYSKLAEE ENATVKILH DFSNGIISE 
      REKERTTNL SQNLASYSK KKRMAMLDL LLAAKNEGA 
      DIDYEGIRE EVDTFMFEG HDTTSMAIS FILLTLANL 
      QDVQTKVRE EILSVVGKE KIPTYNDLQ ELKYTERCI 
      KETLRLFPS VPFISRYAS EDFVTKTGY TIPEGTVLH 
      IHIFDLHRN AEIYPDPLK FDPDRFLPE KVNERHPFA 
      YIPFSAGPR NCIGQKFAF LELKTVLCG ILRKFKLEK 
      VDDMYEIEF RPDLVLRPK NDVKVRIEK L
    CDS sequence
    • >TC012662 RA
      atgctttcc ttttatctg ttattaacc ggtgtcggt 
      ttgattttg ctgctacaa acttggcgt tttttccga 
      aaatatttc aagaataag acaattttg aaaaaaata 
      cgcgggccc cctgaaaca cctattttc ggacacatg 
      tacatcttc acacaagac cctgattta cttttttca 
      aggattcgg aaatattct cgagagtac tacccgatt 
      ttccgcttt tcggccctt tatgtcgac attgttaat 
      tttctagag cctaatgat attgaacta attttatcg 
      accaccaaa catttgagc aagagcaaa atttatact 
      tttttgaac aattggctc ggaacgggc ctcctcaca 
      agcacagga gcgaaatgg caccaaaga cgcaaaatc 
      ctgaccccc gccttccat ttcaacatt ttgcagcaa 
      tttttgaac atttttaac gacgagacc gaaaaattg 
      gtactcaga ctgggtgaa gactgtgga aggaccatc 
      gatgtggta cctccagtt acaaatttt accctccag 
      tcaattgca gaaactgcc atgggtctg agtaacata 
      aacgaactg actcagaag gagtacaag aaggcgatt 
      tacaaaatc gggcatatt ttcctaaaa cggttgtcc 
      aaaccctgg taccgcctt gatgccatc tacaattac 
      agtaaattg gcagaagag gaaaatgca acggttaaa 
      atcttgcac gacttctcc aatggcata atatcagaa 
      cgagagaaa gaaagaacc acgaattta tcgcaaaat 
      ttagcctcg tactccaag aagaaacga atggcaatg 
      ttggacctg ctcctggcc gccaaaaac gaaggcgct 
      gatatcgac tacgagggg attcgtgaa gaagtcgac 
      actttcatg tttgagggt catgacacc acctcgatg 
      gccatctcc ttcatcctc ctaactctg gccaacctc 
      caagacgtc caaaccaaa gtccgcgaa gaaatactt 
      tcagtggtc ggaaaagag aaaatcccc acatacaac 
      gacctccaa gaactcaaa tacaccgag cgctgtatc 
      aaagaaacg ctccgactt ttcccaagc gttcccttt 
      atttcgcgc tacgccagt gaggacttc gtcacgaaa 
      actggttac acgattccc gagggaacg gttttgcat 
      attcatatt tttgatttg caccggaac gcggaaatt 
      tatcctgat cctttgaaa ttcgatccg gatcgtttt 
      cttcccgaa aaagtgaac gagagacac ccttttgcc 
      tatattccg ttcagtgcg ggacctagg aattgtata 
      ggacagaag tttgccttc ctggaactc aaaacagtt 
      ctctgtggg attttacgc aagttcaag ttggaaaag 
      gttgatgat atgtacgaa atcgagttc aggccggat 
      ctagttttg aggcccaaa aatgatgtt aaagtcaga 
      attgaaaaa ttgtag
    mRNA sequence
    • >TC012662 RA
      ctcctgata atctgccac tttgagttg ttaccatta 
      ccacacgct tatgtttgt gtttatttt tacgatgtt 
      acgatgatg atacaactc ctaatctaa tttaaacga 
      ataaataaa acacaggcg ttatctctg tctctgatt 
      aagtattgt tgaactcgc agataacaa aaaaaaaac 
      acagtacgt agttaattg cggcctaaa ttgattatt 
      ccgagcaca aatttgcaa gttgtggtt gttaacaag 
      aagaacaaa gatcggcat tgaaaccaa caattataa 
      aacctgtca gactgcatc caacaccaa cacttgtta 
      cataactct cgcccagtc aacagaatg ctttccttt 
      tatctgtta ttaaccggt gtcggtttg attttgctg 
      ctacaaact tggcgtttt ttccgaaaa tatttcaag 
      aataagaca attttgaaa aaaatacgc gggccccct 
      gaaacacct attttcgga cacatgtac atcttcaca 
      caagaccct gatttactt ttttcaagg attcggaaa 
      tattctcga gagtactac ccgattttc cgcttttcg 
      gccctttat gtcgacatt gttaatttt ctagagcct 
      aatgatatt gaactaatt ttatcgacc accaaacat 
      ttgagcaag agcaaaatt tatactttt ttgaacaat 
      tggctcgga acgggcctc ctcacaagc acaggagcg 
      aaatggcac caaagacgc aaaatcctg acccccgcc 
      ttccatttc aacattttg cagcaattt ttgaacatt 
      tttaacgac gagaccgaa aaattggta ctcagactg 
      ggtgaagac tgtggaagg accatcgat gtggtacct 
      ccagttaca aattttacc ctccagtca attgcagaa 
      actgccatg ggtctgagt aacataaac gaactgact 
      cagaaggag tacaagaag gcgatttac aaaatcggg 
      catattttc ctaaaacgg ttgtccaaa ccctggtac 
      cgccttgat gccatctac aattacagt aaattggca 
      gaagaggaa aatgcaacg gttaaaatc ttgcacgac 
      ttctccaat ggcataata tcagaacga gagaaagaa 
      agaaccacg aatttatcg caaaattta gcctcgtac 
      tccaagaag aaacgaatg gcaatgttg gacctgctc 
      ctggccgcc aaaaacgaa ggcgctgat atcgactac 
      gaggggatt cgtgaagaa gtcgacact ttcatgttt 
      gagggtcat gacaccacc tcgatggcc atctccttc 
      atcctccta actctggcc aacctccaa gacgtccaa 
      accaaagtc cgcgaagaa atactttca gtggtcgga 
      aaagagaaa atccccaca tacaacgac ctccaagaa 
      ctcaaatac accgagcgc tgtatcaaa gaaacgctc 
      cgacttttc ccaagcgtt ccctttatt tcgcgctac 
      gccagtgag gacttcgtc acgaaaact ggttacacg 
      attcccgag ggaacggtt ttgcatatt catattttt 
      gatttgcac cggaacgcg gaaatttat cctgatcct 
      ttgaaattc gatccggat cgttttctt cccgaaaaa 
      gtgaacgag agacaccct tttgcctat attccgttc 
      agtgcggga cctaggaat tgtatagga cagaagttt 
      gccttcctg gaactcaaa acagttctc tgtgggatt 
      ttacgcaag ttcaagttg gaaaaggtt gatgatatg 
      tacgaaatc gagttcagg ccggatcta gttttgagg 
      cccaaaaat gatgttaaa gtcagaatt gaaaaattg 
      tagtgtgtt caataaata aatatttta aggaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY