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Gene information
General information
TC012657 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 12582024..12594828
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012657
    iBeetle screen iB_05287

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012657 RA
      MAATSWMRY PNTRRFNRG RSANKEDDI SIDVNSDSR 
      QNVTNILSA LCQTSGISD GKRLNYLNA FVKVTLHIE 
      GNFESIGFS MGDVLCCLR IALLHESTQ VRSGALRAI 
      RHVLRNEDD IAQLNKLLI PFLIARSLD LLLKKDVER 
      IEAMKLIRK ILLLSPSNF HVALARSLV ALANSVVEE 
      KDRMLRICL ATLCELGVV NTSLFIETG GVAAITRNL 
      LECQTPKIA ESLCGVLLL LLDKPSTRN QAAVDLHSI 
      AAPYCDFHY RHGWKDKDK FVDERELRF NCSRLALLS 
      ILRSWPGIL HFCGPSNKA GLRAVVEVL YLNQLEVRK 
      AVLDLLYEL LGLPQPEWT DELSVALSA VDPCEPQSS 
      WRLNEGFVV AEGRSILPH LAKTTPSIS DMHLSLLLY 
      CFLECGLLG AIVEVIATS DTFISVRAT VLLGELLRL 
      IQVLLPPEC CNISPALPT LLEYATCGK PQAVKAVNG 
      LQQLQKLLR ARPASYSLH LDFILRSGG NVKFSSKNS 
      KKSRHKLKT LKFKLQQLV GKDGDDVIK ETGVLVNKD 
      PYTWNWNLI KIVLKGESL KIDLSETSH RNFIKRLVD 
      FYTPSRNRF SHMDLSTPK VSIAYTLTG IDLINCLLE 
      LEDEESNRM LLDLLSDIS GNINAITSG RSVHDCLFS 
      PQHMNSTQC QSYFLFLGR LSSTDVGMQ LLTSLQIFK 
      QLEHLATTT NNDCYVKLI VSSLDYSNA GPCRDLLTE 
      VLKCAVESS RLYATQFLL VLLRAGINN FADWGVRCL 
      VKQLKDPSR SVFLAALNT LHEACEVES CLETLVKLN 
      PDLGHLGEK GSLLALRLL SITSGFQLL NKNDFVVNE 
      IKRWDEYFI YRYIKLVEG ETSDALTLH QRDEDGKYD 
      KRTSTIRTT NRKDFFLPP HLYGQLARH TDGFLILVQ 
      HGSLENMIQ FLTNGVCET EDDILKLKA SIWALGHFG 
      STSEGLRYL ILHNCLAAL ITLAQECLV FSIRATSYY 
      ALGLIATTR QGADELFKL GWFSTRHNR HVAWPIIEE 
      EAWDEDEDS LRLPSSFPD ITPTLYYFD SGIKDVEGS 
      TDDDTSESM LPDSTVAGP QKSLTLPAN QNQAAGYHK 
      RSLSESKTF EIIRAPQDS KKGSFRLGT EIVRRVRNN 
      STTESTTSG VSSCDSFGG GKSVIYERP QILSPIPSS 
      CSLSTLKQS AKVRRHSES SRRVSVQSG SNSDRSLQS 
      LGGGGSSKL SHQDLVGYA TLRSLRKSP RYLEEPAYD 
      SDDIRSDSS WDVPVSLIE DFNLLNTTG TSIKRFSVH 
      DRNESEDRC YMGVTLPHD INHLFLDVD SEARDASLE 
      RFPIIDDEI VYAMHDFKV REETLWRHD VETCLVCSW 
      KGPKDEVFV TTSSDPLDC KYDPNSPDN VLKGQVLHL 
      IDLFSNPVW AKQVKQSLL QYKQGSPHV FQDACIYSE 
      VCKMISECA YRSSPRRML HELFLEVKF DDLYLQCAR 
      FLKSAAQNN VDSGKSSGD SIDSEGAQS NIKKLDSLK 
      LAYKENKFP IRNRNESKI V
    CDS sequence
    • >TC012657 RA
      atggctgca acgagctgg atgcgatac ccaaacaca 
      cgtcggttt aacagaggc cggtctgcg aacaaagaa 
      gacgacatc agcatcgac gtaaacagc gattctcgg 
      caaaatgta acgaacatc ctgtcagcg ctttgccag 
      acgtcgggt atttcggac ggaaagcgc ctgaattac 
      ctgaacgcg ttcgttaaa gtcacgctg cacattgag 
      ggcaacttc gagagcatt gggtttagc atgggggac 
      gttctgtgc tgcttgcgc atagccctt ctccatgag 
      tcgactcaa gtccggtcg ggggctcta agggccatt 
      cggcacgtc ttgcgcaac gaggacgac attgcccag 
      ctcaacaaa ctcctaatt cctttttta atagccaga 
      tcgttggac ttgttgtta aagaaagac gttgaacga 
      attgaagcg atgaaactc atacggaaa attctccta 
      ctctcgcct tccaacttc cacgtggct ttggcccga 
      agtttggtg gctctggcc aacagcgtg gtcgaggag 
      aaggacagg atgttgcga atctgtctc gcaacgttg 
      tgcgaattg ggggtagtt aacacgagc ctgtttatt 
      gaaactggg ggcgtggcc gccattacc agaaattta 
      ctggagtgt cagacccca aaaatagcg gagtctttg 
      tgtggcgtc ttgctactt ttactggat aaaccatcg 
      actagaaat caggcagcc gttgacttg cactcaatc 
      gccgccccc tactgtgac ttccactac cgccacggt 
      tggaaagac aaggacaaa tttgtggat gagcgtgag 
      ctgcgtttc aattgcagc cgtttagcc cttttgtca 
      attctgcgc agttggcct ggaattttg catttttgt 
      gggccgtcc aacaaggcg gggcttagg gcggtcgtt 
      gaggtgttg tatttgaac caattggaa gttaggaaa 
      gcagttttg gacctattg tacgaacta ctcggcttg 
      cctcagccc gagtggacc gacgagctg tccgtggcg 
      ctgagcgct gtggaccct tgcgagccc caatcttcc 
      tggagactg aacgaaggc tttgtcgtc gccgaaggc 
      cgctcgatt ctacctcac ttagccaaa accacgccc 
      agtataagc gatatgcac ttgtccctc ctgctgtac 
      tgttttctc gaatgcggt ctcttgggc gcgattgtt 
      gaagtgatc gcgactagt gatacgttt ataagcgtt 
      cgagcgacg gtgctgctg ggcgagctt ttacgctta 
      attcaagtt cttcttccg ccagagtgt tgtaatatc 
      tcgccggct cttccaaca cttttggaa tacgcaacg 
      tgtgggaaa ccgcaagct gttaaggct gtcaatggg 
      cttcaacag ttgcaaaag ttgttgagg gccaggccg 
      gcgtcctat agtcttcac ttggatttt attctgaga 
      agtggcggc aatgtcaag ttttcgagt aaaaacagc 
      aagaagagc cgacacaaa ttgaagacg ttgaagttt 
      aagttacag cagcttgtg gggaaagac ggagacgac 
      gtgataaaa gaaaccggg gttttggtg aacaaagac 
      ccatacacg tggaactgg aacctcatc aaaatcgta 
      ctaaagggt gaatcccta aaaatcgac ctttcggag 
      acctcccat cgcaacttc ataaaacgc ctcgtcgac 
      ttctacacc ccttcgcgc aaccgcttc tcccacatg 
      gacttgagc acgcctaaa gtcagtatt gcctacacc 
      ctgaccggg atcgaccta atcaactgt ctcctggag 
      ctcgaagac gaagaaagc aaccgcatg ttgctcgac 
      ctgttgtcg gacatctcg ggcaacatt aatgcgata 
      acgagcggc cgcagcgtc cacgactgt ctcttcagc 
      ccccagcac atgaacagc acccaatgc caaagctac 
      ttcctcttc ctcggccgt ctctcctcc accgacgtg 
      ggaatgcag ctgctgacc agcttacaa atcttcaag 
      caactcgaa cacttggcc acaaccacg aacaacgac 
      tgttacgtc aagttgatc gtgtcgtct ctggactac 
      agcaacgcg gggccttgt cgcgaccta ttgaccgag 
      gtgttaaag tgcgccgtt gaaagttca agactgtac 
      gccacccaa tttctacta gtgctgcta agggccgga 
      attaacaat ttcgccgac tggggcgtt cggtgtcta 
      gtcaaacag ttgaaggat ccgtcgcgg tcggtgttc 
      ctcgccgcg ttaaacaca ctgcatgag gcttgtgaa 
      gtcgaaagc tgtttagag actttagtt aagttaaat 
      ccggacttg ggccatttg ggcgagaaa ggctcgttg 
      ttggcgctc aggttgtta tcgataact tccgggttt 
      caattgttg aacaagaac gacttcgtt gtgaacgaa 
      attaaacga tgggacgag tatttcatt tatagatac 
      attaaacta gtcgagggt gaaacctcc gatgctttg 
      actttacat cagagggac gaggacggg aaatacgat 
      aagaggaca agcacgata aggacgaca aataggaag 
      gattttttc ttgccgccg catctttac ggacaattg 
      gcgcggcac actgatggc tttctcatt ctagtgcag 
      cacgggtcg ctggaaaac atgattcag tttctcacg 
      aatggcgtt tgtgagacc gaggacgat attttgaaa 
      ctgaaagcc tcaatatgg gctttgggc cacttcggc 
      agcaccagc gagggccta agataccta attttgcac 
      aactgtttg gcagctttg ataaccttg gcacaagag 
      tgcttagtg ttctcgatt cgggcaact tcgtattac 
      gccctcggt ctaatcgca acaacgcga caaggcgcc 
      gacgaactc ttcaaactg ggttggttt agcacacgt 
      cataaccga cacgtggcg tggccaatc atcgaagag 
      gaggcgtgg gacgaggac gaggacagt ttacgactt 
      ccgagtagt ttccccgat atcacgccc actttgtat 
      tacttcgac tcgggaatt aaggacgta gagggcagc 
      actgacgac gatacgtcc gagtcaatg ttgcccgat 
      tccacagtg gcaggcccc cagaaaagc ctgactttg 
      ccggccaat cagaaccaa gcggcgggc taccacaag 
      aggtcgctg tccgagtcg aaaaccttc gagattatc 
      cgagcgcct caggacagt aaaaaaggc agcttccga 
      ttgggtacc gagattgtg aggcgcgtt cggaacaat 
      tcgaccacg gaatcgacc acgtccggg gtgagttcg 
      tgcgattcc ttcggcgga ggcaaatct gtgatctac 
      gaacggcca caaatcttg agtcctata ccaagttcc 
      tgtagtctc agtacgtta aaacaatcg gcgaaagtg 
      cggcgtcat tcggaatcg tcacggcgt gtctcagtt 
      caaagcggg tctaactcg gatcgctcg ctccaatcg 
      ctgggcggg ggcgggtcc agtaagctg agccatcag 
      gatctggtt ggctatgcc acacttcgt tccttacga 
      aaatcgccg aggtatttg gaagaaccc gcctacgac 
      tcggacgat atccggagt gatagtagc tgggatgtt 
      cccgtttca ctaatcgaa gatttcaat cttttaaat 
      accactggg acaagtatt aagcggttt agtgtccac 
      gatcggaac gaaagcgag gatcggtgt tacatgggc 
      gtgactttg cctcatgac attaatcat ttgtttctg 
      gatgtggac agtgaggca cgtgatgcc tctttggag 
      agatttccg atcattgac gacgagatt gtctatgca 
      atgcacgat tttaaagtg cgtgaggag acactgtgg 
      aggcacgat gttgaaacg tgccttgtt tgttcgtgg 
      aaggggccc aaagacgag gtttttgtc acgacatct 
      agcgaccct ttggactgt aaatacgac ccaaacagc 
      cctgacaac gttctcaag ggccaggtt ctgcacctg 
      attgaccta ttttcgaac cccgtttgg gccaaacag 
      gtgaaacag tcgctcttg cagtacaag caaggcagt 
      cctcacgtc ttccaagac gcttgtatt tactcagaa 
      gtgtgcaaa atgatttcg gaatgtgcg tatcgttcg 
      tcacctcgt cggatgctc cacgaactc ttcctcgaa 
      gtgaaattc gacgatctc tacctacaa tgcgccagg 
      tttttgaaa agtgccgct cagaataat gtcgattct 
      ggtaagtca tcgggggat tcgatcgat agtgagggg 
      gcgcagtcg aatataaaa aaattagat tcgctaaaa 
      ctcgcatat aaagagaat aaatttccg attcgcaat 
      cgaaacgaa tcgaaaatc gtgtaa
    mRNA sequence
    • >TC012657 RA
      cgcggatac aaatcaaaa acccaattt ccgcaataa 
      attgaaacg cctgactgg gcccaatta taaaaatag 
      tcaagcggc gtcatttcg ccttaatca tcacagaaa 
      aaccgaaaa aaatcggaa aatgtgcgt catgttgtc 
      gcctcacct gttgacagt tggtcagcc tgttttcaa 
      aaaataaaa taaaaaatc gtcgaaact tcgacgtaa 
      cttgcaagt gttgagtcc cacaaagtt tcgttgaat 
      tcgtgaaac tcggaaaaa ttaaacatt gtgggaaaa 
      atcggtggt gcagatgtg gcagcgccg cttgacagt 
      ccatctcct cctcaacgc ctaacctaa ccaccatag 
      tttgacagt tttgtggtt ctaatagtg tacaaaaaa 
      tttgataat gccgtgttt ttattttaa tttataaca 
      ataagtggg actggttgt tttggttag ggtctgggc 
      gcttgggga acatggctg caacgagct ggatgcgat 
      acccaaaca cacgtcggt ttaacagag gccggtctg 
      cgaacaaag aagacgaca tcagcatcg acgtaaaca 
      gcgattctc ggcaaaatg taacgaaca tcctgtcag 
      cgctttgcc agacgtcgg gtatttcgg acggaaagc 
      gcctgaatt acctgaacg cgttcgtta aagtcacgc 
      tgcacattg agggcaact tcgagagca ttgggttta 
      gcatggggg acgttctgt gctgcttgc gcatagccc 
      ttctccatg agtcgactc aagtccggt cgggggctc 
      taagggcca ttcggcacg tcttgcgca acgaggacg 
      acattgccc agctcaaca aactcctaa ttccttttt 
      taatagcca gatcgttgg acttgttgt taaagaaag 
      acgttgaac gaattgaag cgatgaaac tcatacgga 
      aaattctcc tactctcgc cttccaact tccacgtgg 
      ctttggccc gaagtttgg tggctctgg ccaacagcg 
      tggtcgagg agaaggaca ggatgttgc gaatctgtc 
      tcgcaacgt tgtgcgaat tgggggtag ttaacacga 
      gcctgttta ttgaaactg ggggcgtgg ccgccatta 
      ccagaaatt tactggagt gtcagaccc caaaaatag 
      cggagtctt tgtgtggcg tcttgctac ttttactgg 
      ataaaccat cgactagaa atcaggcag ccgttgact 
      tgcactcaa tcgccgccc cctactgtg acttccact 
      accgccacg gttggaaag acaaggaca aatttgtgg 
      atgagcgtg agctgcgtt tcaattgca gccgtttag 
      cccttttgt caattctgc gcagttggc ctggaattt 
      tgcattttt gtgggccgt ccaacaagg cggggctta 
      gggcggtcg ttgaggtgt tgtatttga accaattgg 
      aagttagga aagcagttt tggacctat tgtacgaac 
      tactcggct tgcctcagc ccgagtgga ccgacgagc 
      tgtccgtgg cgctgagcg ctgtggacc cttgcgagc 
      cccaatctt cctggagac tgaacgaag gctttgtcg 
      tcgccgaag gccgctcga ttctacctc acttagcca 
      aaaccacgc ccagtataa gcgatatgc acttgtccc 
      tcctgctgt actgttttc tcgaatgcg gtctcttgg 
      gcgcgattg ttgaagtga tcgcgacta gtgatacgt 
      ttataagcg ttcgagcga cggtgctgc tgggcgagc 
      ttttacgct taattcaag ttcttcttc cgccagagt 
      gttgtaata tctcgccgg ctcttccaa cacttttgg 
      aatacgcaa cgtgtggga aaccgcaag ctgttaagg 
      ctgtcaatg ggcttcaac agttgcaaa agttgttga 
      gggccaggc cggcgtcct atagtcttc acttggatt 
      ttattctga gaagtggcg gcaatgtca agttttcga 
      gtaaaaaca gcaagaaga gccgacaca aattgaaga 
      cgttgaagt ttaagttac agcagcttg tggggaaag 
      acggagacg acgtgataa aagaaaccg gggttttgg 
      tgaacaaag acccataca cgtggaact ggaacctca 
      tcaaaatcg tactaaagg gtgaatccc taaaaatcg 
      acctttcgg agacctccc atcgcaact tcataaaac 
      gcctcgtcg acttctaca ccccttcgc gcaaccgct 
      tctcccaca tggacttga gcacgccta aagtcagta 
      ttgcctaca ccctgaccg ggatcgacc taatcaact 
      gtctcctgg agctcgaag acgaagaaa gcaaccgca 
      tgttgctcg acctgttgt cggacatct cgggcaaca 
      ttaatgcga taacgagcg gccgcagcg tccacgact 
      gtctcttca gcccccagc acatgaaca gcacccaat 
      gccaaagct acttcctct tcctcggcc gtctctcct 
      ccaccgacg tgggaatgc agctgctga ccagcttac 
      aaatcttca agcaactcg aacacttgg ccacaacca 
      cgaacaacg actgttacg tcaagttga tcgtgtcgt 
      ctctggact acagcaacg cggggcctt gtcgcgacc 
      tattgaccg aggtgttaa agtgcgccg ttgaaagtt 
      caagactgt acgccaccc aatttctac tagtgctgc 
      taagggccg gaattaaca atttcgccg actggggcg 
      ttcggtgtc tagtcaaac agttgaagg atccgtcgc 
      ggtcggtgt tcctcgccg cgttaaaca cactgcatg 
      aggcttgtg aagtcgaaa gctgtttag agactttag 
      ttaagttaa atccggact tgggccatt tgggcgaga 
      aaggctcgt tgttggcgc tcaggttgt tatcgataa 
      cttccgggt ttcaattgt tgaacaaga acgacttcg 
      ttgtgaacg aaattaaac gatgggacg agtatttca 
      tttatagat acattaaac tagtcgagg gtgaaacct 
      ccgatgctt tgactttac atcagaggg acgaggacg 
      ggaaatacg ataagagga caagcacga taaggacga 
      caaatagga aggattttt tcttgccgc cgcatcttt 
      acggacaat tggcgcggc acactgatg gctttctca 
      ttctagtgc agcacgggt cgctggaaa acatgattc 
      agtttctca cgaatggcg tttgtgaga ccgaggacg 
      atattttga aactgaaag cctcaatat gggctttgg 
      gccacttcg gcagcacca gcgagggcc taagatacc 
      taattttgc acaactgtt tggcagctt tgataacct 
      tggcacaag agtgcttag tgttctcga ttcgggcaa 
      cttcgtatt acgccctcg gtctaatcg caacaacgc 
      gacaaggcg ccgacgaac tcttcaaac tgggttggt 
      ttagcacac gtcataacc gacacgtgg cgtggccaa 
      tcatcgaag aggaggcgt gggacgagg acgaggaca 
      gtttacgac ttccgagta gtttccccg atatcacgc 
      ccactttgt attacttcg actcgggaa ttaaggacg 
      tagagggca gcactgacg acgatacgt ccgagtcaa 
      tgttgcccg attccacag tggcaggcc cccagaaaa 
      gcctgactt tgccggcca atcagaacc aagcggcgg 
      gctaccaca agaggtcgc tgtccgagt cgaaaacct 
      tcgagatta tccgagcgc ctcaggaca gtaaaaaag 
      gcagcttcc gattgggta ccgagattg tgaggcgcg 
      ttcggaaca attcgacca cggaatcga ccacgtccg 
      gggtgagtt cgtgcgatt ccttcggcg gaggcaaat 
      ctgtgatct acgaacggc cacaaatct tgagtccta 
      taccaagtt cctgtagtc tcagtacgt taaaacaat 
      cggcgaaag tgcggcgtc attcggaat cgtcacggc 
      gtgtctcag ttcaaagcg ggtctaact cggatcgct 
      cgctccaat cgctgggcg ggggcgggt ccagtaagc 
      tgagccatc aggatctgg ttggctatg ccacacttc 
      gttccttac gaaaatcgc cgaggtatt tggaagaac 
      ccgcctacg actcggacg atatccgga gtgatagta 
      gctgggatg ttcccgttt cactaatcg aagatttca 
      atcttttaa ataccactg ggacaagta ttaagcggt 
      ttagtgtcc acgatcgga acgaaagcg aggatcggt 
      gttacatgg gcgtgactt tgcctcatg acattaatc 
      atttgtttc tggatgtgg acagtgagg cacgtgatg 
      cctctttgg agagatttc cgatcattg acgacgaga 
      ttgtctatg caatgcacg attttaaag tgcgtgagg 
      agacactgt ggaggcacg atgttgaaa cgtgccttg 
      tttgttcgt ggaaggggc ccaaagacg aggtttttg 
      tcacgacat ctagcgacc ctttggact gtaaatacg 
      acccaaaca gccctgaca acgttctca agggccagg 
      ttctgcacc tgattgacc tattttcga accccgttt 
      gggccaaac aggtgaaac agtcgctct tgcagtaca 
      agcaaggca gtcctcacg tcttccaag acgcttgta 
      tttactcag aagtgtgca aaatgattt cggaatgtg 
      cgtatcgtt cgtcacctc gtcggatgc tccacgaac 
      tcttcctcg aagtgaaat tcgacgatc tctacctac 
      aatgcgcca ggtttttga aaagtgccg ctcagaata 
      atgtcgatt ctggtaagt catcggggg attcgatcg 
      atagtgagg gggcgcagt cgaatataa aaaaattag 
      attcgctaa aactcgcat ataaagaga ataaatttc 
      cgattcgca atcgaaacg aatcgaaaa tcgtgtaac 
      ttttggttc ctctatttt tcgaattga tacagggtg 
      ttctgtttc taggtttaa gtttttttg cgttacgtg 
      tagtctgtc gctttgttt ttttttgtt gtatataag 
      gaacggttc attccactc gggagaaca acctgtgtg 
      tatataact aatttattt gactttttg tgatcaaaa 
      cagtgaaat tacg
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