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Gene information
General information
TC012616 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 12189944..12197388
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012616
    iBeetle screen iB_05281

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012616 RA
      MLWPGVPLR LALLLLIAT VGPPGVRSE GNVGCLFSE 
      RLCLEDEWC FDDYAFGRC LPPASDVDE DDLYRYSLE 
      REVLHQISR ELRRLFALG YRWSHGYTQ CRLQAMLYA 
      TKHDVSFEN NACSHLVDQ DLEGALKAL EEQDPLDPR 
      EMAIVKYTP SIENPHADF ADEVYYPPV DETPQQALR 
      EKILAPNAI DLYDIEDPY AQVIKRTWI PQMHRRSLP 
      TSDYFTENY NTPSDFRAG RESRDDLEF LPPENDEMT 
      VNDVRQMYP LIRYLNRMK LSPEEIQDT LTPEEYDKL 
      IQLLAQIEA TDPNEEELE TKQADPFER FTLEYNTPD 
      NFRAGWESR DTLSYNDEI PESRIYFDE NDEPSEEYG 
      SGYNIIPGG AFRELKNQF EDNNFNTKE IFRELQHKH 
      RPQNAPGVY TEGGVVYAP RSDDEEQNL EEILTKYNL 
      GFKRPERLD VKKPGPPFD AQIPDGKRA VPNRHQSFH 
      SAFTPPKKE AEGNDVVPQ EPYVVDTDY VYVEFENDV 
      YKWAEGKQL IGNISDLLG LERSAFSHG RVDHNEVTF 
      KVEPNNKGW NSTQVAEQI GKIKDTLRE VTGIVITAA 
      GIGDKTKMR TVVSAPMYD NEFYIVIFM VCGVLGAMI 
      IAASLLILI RRHLKSKEK LQGLSRPDT EASKDYQDL 
      CRARMAAKG QPVGDTVHG RITSLSRES EQSPSSRSS 
      TSSWSEEPA LHNMDISTG HMVLSYMED HLKNKDRLE 
      QEWVALCAY VAEPCDTSI ATKKENLEK NRYPDIIPY 
      DHSRVVLNE LSNASGSDY INASSITDH DPRNPAYIA 
      TQGPLPLTA PDFWQLIWE QGAVVIVML TRLTEGGAA 
      MCHRYWPEE GSELYHIYE VHLVSEHIW CDDYLVRSF 
      YLKNVKTGE TRTVTQFHF LSWPESGVP ASTKALLEF 
      RRKVNKSYR GRSCPIVVH CSDGAGRTG TYCLIDMVL 
      SRMAKGAKE IDIAATLEH LRDQRPRMV ATKQQFEFV 
      LTAVAEEVH AILKALPPQ PSPAQADKD KQ
    CDS sequence
    • >TC012616 RA
      atgctgtgg ccgggcgta ccgttacgg ctcgctctt 
      cttctcctg attgccacc gtcgggcca ccgggagtt 
      cgttccgaa gggaacgtg ggctgttta ttcagcgaa 
      cgattgtgc ttggaggac gaatggtgc tttgacgat 
      tacgctttt ggtcgatgt ttaccgcca gcttcggat 
      gtcgacgag gatgacttg tatagatac agtctcgag 
      cgagaagtt ttgcaccag ataagtcga gaattgcgc 
      cgattgttt gcgttggga tatcgatgg agccacggc 
      tacacgcaa tgtcgctta caggcgatg ttatatgcc 
      accaagcac gatgtatcg ttcgagaac aatgcttgc 
      tcccattta gtcgaccag gacttggaa ggagctttg 
      aaagctcta gaagaacaa gatccatta gatccgaga 
      gaaatggct attgtcaaa tacactcct agtattgaa 
      aacccacat gctgacttt gctgacgaa gtttattac 
      cccccagta gacgaaacc ccccaacaa gccctccga 
      gagaaaatt ctagcacca aatgctata gacctttac 
      gacatagag gatccttac gcacaagtc attaaacgg 
      acatggata ccacaaatg catcgccgt tctcttccc 
      acttcggat tattttact gaaaattac aacacacct 
      agcgatttc cgagcagga agagaatct agagacgac 
      ctcgagttt cttccaccc gaaaacgac gaaatgaca 
      gtgaatgac gttcgccaa atgtaccct ctcatcaga 
      tacctgaac cgaatgaag ctaagtccg gaagaaatc 
      caagacact ctcaccccc gaagaatac gacaaactc 
      atccaactg ttagcacaa atcgaagca actgacccg 
      aatgaagaa gaactcgaa accaaacaa gccgaccct 
      ttcgaacgc ttcactctg gaatacaac actccagac 
      aacttccgc gccggctgg gaatcccgc gacacccta 
      agctacaat gacgaaatc ccagaatca cgaatttat 
      ttcgacgaa aacgacgag ccaagcgag gaatacgga 
      agtggctac aatataatc ccgggcggt gctttccga 
      gaattgaaa aatcaattc gaagacaat aattttaac 
      acgaaggag atcttcaga gaattgcag cataaacac 
      cggccgcag aacgctccg ggtgtgtac accgagggc 
      ggagttgtc tacgcccct cgttccgac gacgaggag 
      caaaatctc gaggaaatt ttaaccaag tacaatctg 
      gggtttaaa cgacccgaa agactcgat gtaaaaaag 
      ccggggcca ccgttcgac gcacaaata cccgacggg 
      aaaagagca gtgcccaac cgacaccaa agcttccac 
      tcggctttc acgccgccg aaaaaggaa gccgaaggc 
      aacgacgtc gtcccgcaa gagccttac gttgtcgac 
      acggactac gtttacgtc gaatttgaa aacgatgtg 
      tataagtgg gcggaaggt aagcagttg atcggaaat 
      atttccgat cttctggga cttgagcgg tcggctttc 
      agccatgga cgcgtggac cataatgag gttactttc 
      aaagtagaa ccgaacaac aagggttgg aattcgaca 
      caagtggcc gaacaaatc ggtaagatc aaggacact 
      ctcagagaa gttacaggt attgtaatt acggcagct 
      ggaattggt gacaagact aagatgcga actgttgta 
      tctgcccca atgtacgac aacgaattt tacatcgtg 
      atcttcatg gtttgtgga gtattagga gctatgatc 
      attgcggca tcacttctg atcctgatc cggcgacat 
      ttgaagtct aaggaaaag ttacaggga ttgtcgcgt 
      ccagacacc gaggccagt aaagattat caagatttg 
      tgccgcgct cgaatggct gccaaaggc caacccgtc 
      ggagatacg gtccatggc agaattaca tccctgtca 
      cgtgaatcc gagcaaagt cccagcagc cgctcgagc 
      acatcatca tggagcgag gagccagct ttgcacaat 
      atggacatt tccaccggt catatggtc ctaagctac 
      atggaggac cacttgaag aacaaggac cgtctggag 
      caggaatgg gtggccctg tgcgcctac gtggccgaa 
      ccttgcgac accagcatc gccaccaag aaggagaac 
      ttggagaag aaccgctac ccggacatc atcccctac 
      gaccactcc cgcgtcgtc ctcaacgaa ctctccaat 
      gcttcagga tccgactac atcaacgct tccagcatc 
      acggaccac gacccccgc aaccccgcc tacatcgcc 
      acccagggg ccgctcccg ctcaccgcc cccgacttc 
      tggcagctg atctgggag cagggcgcc gtcgtgatc 
      gtgatgctg acgcggctg acggagggc ggcgccgcc 
      atgtgccac cgctactgg cccgaggag ggctccgag 
      ctgtaccac atctacgag gtgcacctc gtcagcgag 
      cacatctgg tgcgacgat tacctagtc cgttcgttc 
      tacttgaaa aatgtcaaa accggagag acgcgcacc 
      gtcacccag ttccacttc ctgtcctgg cccgagtcg 
      ggcgtcccg gcgtccacc aaggccctg ctggagttc 
      cgtcggaag gtgaataag tcgtacaga gggcggtcg 
      tgccccatc gtggtgcac tgcagcgac ggggccggg 
      aggacgggc acctactgc ttgatcgat atggtgctg 
      agccggatg gcaaagggc gccaaggag attgatatc 
      gcagcgact ttggaacat ctgcgggat cagaggccg 
      agaatggtt gccaccaag cagcaattt gagttcgtt 
      ctgacagct gttgctgaa gaagtccac gccatttta 
      aaagctttg ccaccgcaa ccttcgccc gctcaggct 
      gacaaagac aaacaataa
    mRNA sequence
    • >TC012616 RA
      caacagacg acttaatac cacaatcgg gaaaattta 
      gagggctag gtgacggcg tccccgtta ttgcccacc 
      gaattcacc accaatttc ttgttgttt tgtgatcac 
      gggatcaaa ggaagcgat gcagtgaac ggcttcggc 
      ttgcacacc aggccatgc tgtggccgg gcgtaccgt 
      tacggctcg ctcttcttc tcctgattg ccaccgtcg 
      ggccaccgg gagttcgtt ccgaaggga acgtgggct 
      gtttattca gcgaacgat tgtgcttgg aggacgaat 
      ggtgctttg acgattacg cttttggtc gatgtttac 
      cgccagctt cggatgtcg acgaggatg acttgtata 
      gatacagtc tcgagcgag aagttttgc accagataa 
      gtcgagaat tgcgccgat tgtttgcgt tgggatatc 
      gatggagcc acggctaca cgcaatgtc gcttacagg 
      cgatgttat atgccacca agcacgatg tatcgttcg 
      agaacaatg cttgctccc atttagtcg accaggact 
      tggaaggag ctttgaaag ctctagaag aacaagatc 
      cattagatc cgagagaaa tggctattg tcaaataca 
      ctcctagta ttgaaaacc cacatgctg actttgctg 
      acgaagttt attaccccc cagtagacg aaacccccc 
      aacaagccc tccgagaga aaattctag caccaaatg 
      ctatagacc tttacgaca tagaggatc cttacgcac 
      aagtcatta aacggacat ggataccac aaatgcatc 
      gccgttctc ttcccactt cggattatt ttactgaaa 
      attacaaca cacctagcg atttccgag caggaagag 
      aatctagag acgacctcg agtttcttc cacccgaaa 
      acgacgaaa tgacagtga atgacgttc gccaaatgt 
      accctctca tcagatacc tgaaccgaa tgaagctaa 
      gtccggaag aaatccaag acactctca cccccgaag 
      aatacgaca aactcatcc aactgttag cacaaatcg 
      aagcaactg acccgaatg aagaagaac tcgaaacca 
      aacaagccg accctttcg aacgcttca ctctggaat 
      acaacactc cagacaact tccgcgccg gctgggaat 
      cccgcgaca ccctaagct acaatgacg aaatcccag 
      aatcacgaa tttatttcg acgaaaacg acgagccaa 
      gcgaggaat acggaagtg gctacaata taatcccgg 
      gcggtgctt tccgagaat tgaaaaatc aattcgaag 
      acaataatt ttaacacga aggagatct tcagagaat 
      tgcagcata aacaccggc cgcagaacg ctccgggtg 
      tgtacaccg agggcggag ttgtctacg cccctcgtt 
      ccgacgacg aggagcaaa atctcgagg aaattttaa 
      ccaagtaca atctggggt ttaaacgac ccgaaagac 
      tcgatgtaa aaaagccgg ggccaccgt tcgacgcac 
      aaatacccg acgggaaaa gagcagtgc ccaaccgac 
      accaaagct tccactcgg ctttcacgc cgccgaaaa 
      aggaagccg aaggcaacg acgtcgtcc cgcaagagc 
      cttacgttg tcgacacgg actacgttt acgtcgaat 
      ttgaaaacg atgtgtata agtgggcgg aaggtaagc 
      agttgatcg gaaatattt ccgatcttc tgggacttg 
      agcggtcgg ctttcagcc atggacgcg tggaccata 
      atgaggtta ctttcaaag tagaaccga acaacaagg 
      gttggaatt cgacacaag tggccgaac aaatcggta 
      agatcaagg acactctca gagaagtta caggtattg 
      taattacgg cagctggaa ttggtgaca agactaaga 
      tgcgaactg ttgtatctg ccccaatgt acgacaacg 
      aattttaca tcgtgatct tcatggttt gtggagtat 
      taggagcta tgatcattg cggcatcac ttctgatcc 
      tgatccggc gacatttga agtctaagg aaaagttac 
      agggattgt cgcgtccag acaccgagg ccagtaaag 
      attatcaag atttgtgcc gcgctcgaa tggctgcca 
      aaggccaac ccgtcggag atacggtcc atggcagaa 
      ttacatccc tgtcacgtg aatccgagc aaagtccca 
      gcagccgct cgagcacat catcatgga gcgaggagc 
      cagctttgc acaatatgg acatttcca ccggtcata 
      tggtcctaa gctacatgg aggaccact tgaagaaca 
      aggaccgtc tggagcagg aatgggtgg ccctgtgcg 
      cctacgtgg ccgaacctt gcgacacca gcatcgcca 
      ccaagaagg agaacttgg agaagaacc gctacccgg 
      acatcatcc cctacgacc actcccgcg tcgtcctca 
      acgaactct ccaatgctt caggatccg actacatca 
      acgcttcca gcatcacgg accacgacc cccgcaacc 
      ccgcctaca tcgccaccc aggggccgc tcccgctca 
      ccgcccccg acttctggc agctgatct gggagcagg 
      gcgccgtcg tgatcgtga tgctgacgc ggctgacgg 
      agggcggcg ccgccatgt gccaccgct actggcccg 
      aggagggct ccgagctgt accacatct acgaggtgc 
      acctcgtca gcgagcaca tctggtgcg acgattacc 
      tagtccgtt cgttctact tgaaaaatg tcaaaaccg 
      gagagacgc gcaccgtca cccagttcc acttcctgt 
      cctggcccg agtcgggcg tcccggcgt ccaccaagg 
      ccctgctgg agttccgtc ggaaggtga ataagtcgt 
      acagagggc ggtcgtgcc ccatcgtgg tgcactgca 
      gcgacgggg ccgggagga cgggcacct actgcttga 
      tcgatatgg tgctgagcc ggatggcaa agggcgcca 
      aggagattg atatcgcag cgactttgg aacatctgc 
      gggatcaga ggccgagaa tggttgcca ccaagcagc 
      aatttgagt tcgttctga cagctgttg ctgaagaag 
      tccacgcca ttttaaaag ctttgccac cgcaacctt 
      cgcccgctc aggctgaca aagacaaac aataaaccg 
      atctgatta agagcaact cccaacaac cagccctgt 
      tcggggtta ctcggcttt tctgtttcg gacctgacg 
      tattcttca tcttcacac acacgtacc ttgctcttt 
      ggttcacct ctatgatta catatccat gggttaagc 
      agattctcc gatttattt ttctcacat aaaaacacg 
      tctatattg atgttactt catatgtta caacttata 
      taattatct gtaaagaat atatatttt gtgtataaa 
      tgtatcaac gttacgcta catattttt cttatttgt 
      ggaatattt cagtcaatt cgaaaggat atcgcgttt 
      ccagtcacg agcctacaa tcagcaacc aagtggagg 
      aattacgag tattaatat ttttggtca ccagcctcc 
      caaaaatgt tgatactga cgatttttt ttcttactt 
      tttttctct tgattaaac tcgacgctt accataata 
      taattcaga ctgaagtta atgagaaat gtaaacttt 
      atttatttt ttatgaagt tttattaaa atgataaag 
      agaaggctg aggcggggg aaattccct tttatccac 
      ttttatcac tacgggaac gtccctggt cccagctgc 
      ttatattat gacgagaga tgacaaaac ttaaaacga 
      ttagacttt taagagaat ttaatgata aaaaggtat 
      aattacaaa tgttctatg ctatattgt caactaact 
      atattttgt cagataatg ttatgtatt gaatcatta 
      atgtactga atttatctg aattagaat agtgaaatg 
      taaactaat
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