| General information | |||
| TC012616 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 12189944..12197388 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC012616 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05281 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC012616 RA
MLWPGVPLR LALLLLIAT VGPPGVRSE GNVGCLFSE RLCLEDEWC FDDYAFGRC LPPASDVDE DDLYRYSLE REVLHQISR ELRRLFALG YRWSHGYTQ CRLQAMLYA TKHDVSFEN NACSHLVDQ DLEGALKAL EEQDPLDPR EMAIVKYTP SIENPHADF ADEVYYPPV DETPQQALR EKILAPNAI DLYDIEDPY AQVIKRTWI PQMHRRSLP TSDYFTENY NTPSDFRAG RESRDDLEF LPPENDEMT VNDVRQMYP LIRYLNRMK LSPEEIQDT LTPEEYDKL IQLLAQIEA TDPNEEELE TKQADPFER FTLEYNTPD NFRAGWESR DTLSYNDEI PESRIYFDE NDEPSEEYG SGYNIIPGG AFRELKNQF EDNNFNTKE IFRELQHKH RPQNAPGVY TEGGVVYAP RSDDEEQNL EEILTKYNL GFKRPERLD VKKPGPPFD AQIPDGKRA VPNRHQSFH SAFTPPKKE AEGNDVVPQ EPYVVDTDY VYVEFENDV YKWAEGKQL IGNISDLLG LERSAFSHG RVDHNEVTF KVEPNNKGW NSTQVAEQI GKIKDTLRE VTGIVITAA GIGDKTKMR TVVSAPMYD NEFYIVIFM VCGVLGAMI IAASLLILI RRHLKSKEK LQGLSRPDT EASKDYQDL CRARMAAKG QPVGDTVHG RITSLSRES EQSPSSRSS TSSWSEEPA LHNMDISTG HMVLSYMED HLKNKDRLE QEWVALCAY VAEPCDTSI ATKKENLEK NRYPDIIPY DHSRVVLNE LSNASGSDY INASSITDH DPRNPAYIA TQGPLPLTA PDFWQLIWE QGAVVIVML TRLTEGGAA MCHRYWPEE GSELYHIYE VHLVSEHIW CDDYLVRSF YLKNVKTGE TRTVTQFHF LSWPESGVP ASTKALLEF RRKVNKSYR GRSCPIVVH CSDGAGRTG TYCLIDMVL SRMAKGAKE IDIAATLEH LRDQRPRMV ATKQQFEFV LTAVAEEVH AILKALPPQ PSPAQADKD KQ
- >TC012616 RA
atgctgtgg ccgggcgta ccgttacgg ctcgctctt cttctcctg attgccacc gtcgggcca ccgggagtt cgttccgaa gggaacgtg ggctgttta ttcagcgaa cgattgtgc ttggaggac gaatggtgc tttgacgat tacgctttt ggtcgatgt ttaccgcca gcttcggat gtcgacgag gatgacttg tatagatac agtctcgag cgagaagtt ttgcaccag ataagtcga gaattgcgc cgattgttt gcgttggga tatcgatgg agccacggc tacacgcaa tgtcgctta caggcgatg ttatatgcc accaagcac gatgtatcg ttcgagaac aatgcttgc tcccattta gtcgaccag gacttggaa ggagctttg aaagctcta gaagaacaa gatccatta gatccgaga gaaatggct attgtcaaa tacactcct agtattgaa aacccacat gctgacttt gctgacgaa gtttattac cccccagta gacgaaacc ccccaacaa gccctccga gagaaaatt ctagcacca aatgctata gacctttac gacatagag gatccttac gcacaagtc attaaacgg acatggata ccacaaatg catcgccgt tctcttccc acttcggat tattttact gaaaattac aacacacct agcgatttc cgagcagga agagaatct agagacgac ctcgagttt cttccaccc gaaaacgac gaaatgaca gtgaatgac gttcgccaa atgtaccct ctcatcaga tacctgaac cgaatgaag ctaagtccg gaagaaatc caagacact ctcaccccc gaagaatac gacaaactc atccaactg ttagcacaa atcgaagca actgacccg aatgaagaa gaactcgaa accaaacaa gccgaccct ttcgaacgc ttcactctg gaatacaac actccagac aacttccgc gccggctgg gaatcccgc gacacccta agctacaat gacgaaatc ccagaatca cgaatttat ttcgacgaa aacgacgag ccaagcgag gaatacgga agtggctac aatataatc ccgggcggt gctttccga gaattgaaa aatcaattc gaagacaat aattttaac acgaaggag atcttcaga gaattgcag cataaacac cggccgcag aacgctccg ggtgtgtac accgagggc ggagttgtc tacgcccct cgttccgac gacgaggag caaaatctc gaggaaatt ttaaccaag tacaatctg gggtttaaa cgacccgaa agactcgat gtaaaaaag ccggggcca ccgttcgac gcacaaata cccgacggg aaaagagca gtgcccaac cgacaccaa agcttccac tcggctttc acgccgccg aaaaaggaa gccgaaggc aacgacgtc gtcccgcaa gagccttac gttgtcgac acggactac gtttacgtc gaatttgaa aacgatgtg tataagtgg gcggaaggt aagcagttg atcggaaat atttccgat cttctggga cttgagcgg tcggctttc agccatgga cgcgtggac cataatgag gttactttc aaagtagaa ccgaacaac aagggttgg aattcgaca caagtggcc gaacaaatc ggtaagatc aaggacact ctcagagaa gttacaggt attgtaatt acggcagct ggaattggt gacaagact aagatgcga actgttgta tctgcccca atgtacgac aacgaattt tacatcgtg atcttcatg gtttgtgga gtattagga gctatgatc attgcggca tcacttctg atcctgatc cggcgacat ttgaagtct aaggaaaag ttacaggga ttgtcgcgt ccagacacc gaggccagt aaagattat caagatttg tgccgcgct cgaatggct gccaaaggc caacccgtc ggagatacg gtccatggc agaattaca tccctgtca cgtgaatcc gagcaaagt cccagcagc cgctcgagc acatcatca tggagcgag gagccagct ttgcacaat atggacatt tccaccggt catatggtc ctaagctac atggaggac cacttgaag aacaaggac cgtctggag caggaatgg gtggccctg tgcgcctac gtggccgaa ccttgcgac accagcatc gccaccaag aaggagaac ttggagaag aaccgctac ccggacatc atcccctac gaccactcc cgcgtcgtc ctcaacgaa ctctccaat gcttcagga tccgactac atcaacgct tccagcatc acggaccac gacccccgc aaccccgcc tacatcgcc acccagggg ccgctcccg ctcaccgcc cccgacttc tggcagctg atctgggag cagggcgcc gtcgtgatc gtgatgctg acgcggctg acggagggc ggcgccgcc atgtgccac cgctactgg cccgaggag ggctccgag ctgtaccac atctacgag gtgcacctc gtcagcgag cacatctgg tgcgacgat tacctagtc cgttcgttc tacttgaaa aatgtcaaa accggagag acgcgcacc gtcacccag ttccacttc ctgtcctgg cccgagtcg ggcgtcccg gcgtccacc aaggccctg ctggagttc cgtcggaag gtgaataag tcgtacaga gggcggtcg tgccccatc gtggtgcac tgcagcgac ggggccggg aggacgggc acctactgc ttgatcgat atggtgctg agccggatg gcaaagggc gccaaggag attgatatc gcagcgact ttggaacat ctgcgggat cagaggccg agaatggtt gccaccaag cagcaattt gagttcgtt ctgacagct gttgctgaa gaagtccac gccatttta aaagctttg ccaccgcaa ccttcgccc gctcaggct gacaaagac aaacaataa
- >TC012616 RA
caacagacg acttaatac cacaatcgg gaaaattta gagggctag gtgacggcg tccccgtta ttgcccacc gaattcacc accaatttc ttgttgttt tgtgatcac gggatcaaa ggaagcgat gcagtgaac ggcttcggc ttgcacacc aggccatgc tgtggccgg gcgtaccgt tacggctcg ctcttcttc tcctgattg ccaccgtcg ggccaccgg gagttcgtt ccgaaggga acgtgggct gtttattca gcgaacgat tgtgcttgg aggacgaat ggtgctttg acgattacg cttttggtc gatgtttac cgccagctt cggatgtcg acgaggatg acttgtata gatacagtc tcgagcgag aagttttgc accagataa gtcgagaat tgcgccgat tgtttgcgt tgggatatc gatggagcc acggctaca cgcaatgtc gcttacagg cgatgttat atgccacca agcacgatg tatcgttcg agaacaatg cttgctccc atttagtcg accaggact tggaaggag ctttgaaag ctctagaag aacaagatc cattagatc cgagagaaa tggctattg tcaaataca ctcctagta ttgaaaacc cacatgctg actttgctg acgaagttt attaccccc cagtagacg aaacccccc aacaagccc tccgagaga aaattctag caccaaatg ctatagacc tttacgaca tagaggatc cttacgcac aagtcatta aacggacat ggataccac aaatgcatc gccgttctc ttcccactt cggattatt ttactgaaa attacaaca cacctagcg atttccgag caggaagag aatctagag acgacctcg agtttcttc cacccgaaa acgacgaaa tgacagtga atgacgttc gccaaatgt accctctca tcagatacc tgaaccgaa tgaagctaa gtccggaag aaatccaag acactctca cccccgaag aatacgaca aactcatcc aactgttag cacaaatcg aagcaactg acccgaatg aagaagaac tcgaaacca aacaagccg accctttcg aacgcttca ctctggaat acaacactc cagacaact tccgcgccg gctgggaat cccgcgaca ccctaagct acaatgacg aaatcccag aatcacgaa tttatttcg acgaaaacg acgagccaa gcgaggaat acggaagtg gctacaata taatcccgg gcggtgctt tccgagaat tgaaaaatc aattcgaag acaataatt ttaacacga aggagatct tcagagaat tgcagcata aacaccggc cgcagaacg ctccgggtg tgtacaccg agggcggag ttgtctacg cccctcgtt ccgacgacg aggagcaaa atctcgagg aaattttaa ccaagtaca atctggggt ttaaacgac ccgaaagac tcgatgtaa aaaagccgg ggccaccgt tcgacgcac aaatacccg acgggaaaa gagcagtgc ccaaccgac accaaagct tccactcgg ctttcacgc cgccgaaaa aggaagccg aaggcaacg acgtcgtcc cgcaagagc cttacgttg tcgacacgg actacgttt acgtcgaat ttgaaaacg atgtgtata agtgggcgg aaggtaagc agttgatcg gaaatattt ccgatcttc tgggacttg agcggtcgg ctttcagcc atggacgcg tggaccata atgaggtta ctttcaaag tagaaccga acaacaagg gttggaatt cgacacaag tggccgaac aaatcggta agatcaagg acactctca gagaagtta caggtattg taattacgg cagctggaa ttggtgaca agactaaga tgcgaactg ttgtatctg ccccaatgt acgacaacg aattttaca tcgtgatct tcatggttt gtggagtat taggagcta tgatcattg cggcatcac ttctgatcc tgatccggc gacatttga agtctaagg aaaagttac agggattgt cgcgtccag acaccgagg ccagtaaag attatcaag atttgtgcc gcgctcgaa tggctgcca aaggccaac ccgtcggag atacggtcc atggcagaa ttacatccc tgtcacgtg aatccgagc aaagtccca gcagccgct cgagcacat catcatgga gcgaggagc cagctttgc acaatatgg acatttcca ccggtcata tggtcctaa gctacatgg aggaccact tgaagaaca aggaccgtc tggagcagg aatgggtgg ccctgtgcg cctacgtgg ccgaacctt gcgacacca gcatcgcca ccaagaagg agaacttgg agaagaacc gctacccgg acatcatcc cctacgacc actcccgcg tcgtcctca acgaactct ccaatgctt caggatccg actacatca acgcttcca gcatcacgg accacgacc cccgcaacc ccgcctaca tcgccaccc aggggccgc tcccgctca ccgcccccg acttctggc agctgatct gggagcagg gcgccgtcg tgatcgtga tgctgacgc ggctgacgg agggcggcg ccgccatgt gccaccgct actggcccg aggagggct ccgagctgt accacatct acgaggtgc acctcgtca gcgagcaca tctggtgcg acgattacc tagtccgtt cgttctact tgaaaaatg tcaaaaccg gagagacgc gcaccgtca cccagttcc acttcctgt cctggcccg agtcgggcg tcccggcgt ccaccaagg ccctgctgg agttccgtc ggaaggtga ataagtcgt acagagggc ggtcgtgcc ccatcgtgg tgcactgca gcgacgggg ccgggagga cgggcacct actgcttga tcgatatgg tgctgagcc ggatggcaa agggcgcca aggagattg atatcgcag cgactttgg aacatctgc gggatcaga ggccgagaa tggttgcca ccaagcagc aatttgagt tcgttctga cagctgttg ctgaagaag tccacgcca ttttaaaag ctttgccac cgcaacctt cgcccgctc aggctgaca aagacaaac aataaaccg atctgatta agagcaact cccaacaac cagccctgt tcggggtta ctcggcttt tctgtttcg gacctgacg tattcttca tcttcacac acacgtacc ttgctcttt ggttcacct ctatgatta catatccat gggttaagc agattctcc gatttattt ttctcacat aaaaacacg tctatattg atgttactt catatgtta caacttata taattatct gtaaagaat atatatttt gtgtataaa tgtatcaac gttacgcta catattttt cttatttgt ggaatattt cagtcaatt cgaaaggat atcgcgttt ccagtcacg agcctacaa tcagcaacc aagtggagg aattacgag tattaatat ttttggtca ccagcctcc caaaaatgt tgatactga cgatttttt ttcttactt tttttctct tgattaaac tcgacgctt accataata taattcaga ctgaagtta atgagaaat gtaaacttt atttatttt ttatgaagt tttattaaa atgataaag agaaggctg aggcggggg aaattccct tttatccac ttttatcac tacgggaac gtccctggt cccagctgc ttatattat gacgagaga tgacaaaac ttaaaacga ttagacttt taagagaat ttaatgata aaaaggtat aattacaaa tgttctatg ctatattgt caactaact atattttgt cagataatg ttatgtatt gaatcatta atgtactga atttatctg aattagaat agtgaaatg taaactaat
