Status
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Gene information
General information
TC034405
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC012615
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 12184444..12189585
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034405
    iBeetle screen iB_05280

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 87.5% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection



    Pupal morphology
    elytra blistered - Number of animals showing the phenotype: 5

    oblique dorsal thoracic muscles median ap-orientation irregular - Number of animals showing the phenotype: 1

    Adult morphology
    elytra blistered - Number of animals showing the phenotype: 3

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034405 RA
      MGTSDAVEH SLASGVDMR ASEVRVADD YTKRKHVLR 
      VSSVNPCRS EFLLQAENT EEFTDWFKA LQEQMATNT 
      EAEAKFDPS TGSKQQAVP QTIPASTSI LVQGNSRLS 
      PQPTKCKSS TSRNRSPTG QSPVSKNRK PSQVADPST 
      SPKVKTWRG RVAQQFRRM QGASSPSSP TAPEGSTFG 
      IPIEQCLTS STNPYVPRF VEVCTDIVD AHGLQTVGI 
      YRVPGNNAS IVALIDEIN RNYEEVPVD DPRWNDLHV 
      VSSLLKAFF RKIPDSLVT SALYPSFIK ADKIENPEA 
      RMKELKRLI KSLPPHNYH TLKHIMFHL KKVMDNSEI 
      NKMEAKNLA IVFGPNIVR PEDDNMETM VSNMTHQYK 
      IVETLLTSA DWFFSDDDT DEVPLSAVI TEAQDEPEP 
      PSSQALLLE NISKYEALK DKQKNGALL SSIISAAQR 
      KVKRKTTKG SGSLSESKE VTSPNGTGG YYSDTFSQS 
      DLKNSTITD DKSNVIPTT VSEIEKSCK QPAKVPWFN 
      YSTDKEDFH RRIENFKQE TEAMLQMPR HLEISVTNI 
      ASKSTSASN VNQTPRLST RTVDSHLLK TNSASNVFT 
      RTINMDNRH SLDNSYQVQ YNKNNSDFG GSLRDTNRN 
      ESVSTQNGR LVRRGSSVE NVNSSSMDL NANGAMKKV 
      KYENEGGSL DSLHKEVSI DDDADLVTT LTNLYDKKL 
      MEKKNSREN LLSGEDFPY ADESPEKTH RIIAHSLSD 
      KENVPNLGD CYRNPSLHK NQFSNKANF LHWDDSKLK 
      ESKNDTEKD EDSTMTSEK HISNKHMNN RKNSVPNSK 
      LRRSESLNK PERTVSPLN NKLKRSESL NKTGDKLKR 
      SDSLTKTEK TESNLNKRR ELATSVRRS KDVTKLKRK 
      NGMPDRSIK RRHTVGGTK DPDKVTWLD NKYQEEVTS 
      NKENKEKNL RTSSPDLSS TRRERLFFE INLIGPENM 
      VVALKQHLM GARPQSFPE TAVFKVPLE SHV
    CDS sequence
    • >TC034405 RA
      atgggtact tccgacgcc gttgaacat tcactagcc 
      agtggcgtg gacatgagg gccagcgaa gttcgcgta 
      gctgatgat tacaccaaa cgaaaacat gtcttgagg 
      gtatcatca gtgaatcct tgtcggtcg gaattcctc 
      ctccaagcc gaaaacaca gaagaattc actgactgg 
      ttcaaggct ttgcaagaa caaatggcc acgaatacc 
      gaggccgaa gcgaaattc gacccatcg actggcagt 
      aaacagcaa gccgtacca cagaccatc ccagcgtcc 
      acttccatc ctagtccaa ggcaacagc cgcctctcc 
      cctcagccc accaaatgc aaatcctcc acctcccgc 
      aaccgttcc ccaaccggt caatccccc gtgagcaaa 
      aaccgcaaa ccgtctcaa gtggccgac ccctccacc 
      agccctaaa gtgaagaca tggcggggt cgcgtggcc 
      caacagttc cggcgaatg caaggtgca agttcgccg 
      agttccccg acggctccc gaaggctca actttcggg 
      attcccatc gaacagtgt ctcacatct tcgacgaat 
      ccctacgtt ccgaggttc gtcgaagtg tgtacggac 
      attgtcgac gctcacgga ttacaaaca gtggggata 
      taccgagtg ccgggaaat aacgcctcg attgtagcg 
      ttgatcgac gaaatcaac aggaattac gaagaagtg 
      cctgtggat gatccacga tggaacgac ctacacgtt 
      gttagtagc cttctgaag gccttcttc aggaaaatc 
      cccgattct ttagtgact agtgcgttg tatccgagt 
      ttcattaag gccgacaaa atcgagaat cccgaagcc 
      aggatgaag gaattgaag aggctgatt aagagtttg 
      ccccctcac aattaccac acgcttaaa cacatcatg 
      tttcacttg aagaaagtt atggataat tctgagatt 
      aataaaatg gaagcgaaa aatttagcg attgtcttc 
      ggcccgaac attgtcagg cccgaggac gacaatatg 
      gaaacaatg gtcagtaac atgacccat cagtataaa 
      atcgtggaa actctactg acaagcgcc gattggttc 
      ttttcggac gacgacacg gacgaagtg cctctttcc 
      gccgttata accgaagcg caggacgag ccagaaccc 
      ccgagtagt caagctctg ttactcgaa aatattagc 
      aaatatgaa gctttaaag gataagcaa aagaatggc 
      gcattgctt tcatcgatc atttcggcg gcgcagcga 
      aaagtgaaa aggaaaacg accaaaggt tcaggttcc 
      ttaagcgaa agcaaagaa gtcacgtct ccgaacgga 
      accggaggc tactattcg gacacattc tcacaatct 
      gatctcaaa aatagtaca attacggac gataaaagt 
      aacgtcata cccacaaca gtgagcgaa atcgaaaag 
      agttgcaaa cagcctgca aaagtgcct tggtttaat 
      tactcgact gataaagag gattttcac cgacgtatt 
      gaaaacttc aaacaggag acggaagct atgttgcaa 
      atgccgcga catttggag atttcggtg accaacatc 
      gcttccaaa agtacttcg gctagtaac gtaaatcaa 
      acaccccga ctgtccaca agaacggtc gactcccat 
      ttacttaaa acaaacagt gcttccaat gtatttaca 
      cgaactatc aacatggac aatagacac agtttggac 
      aattcgtac caagtgcaa tacaataaa aacaatagt 
      gatttcggt ggcagttta cgcgacacg aacagaaac 
      gagagtgtt agtacgcaa aacggccgg ttggtgcga 
      cgtggcagt tccgtcgag aacgtcaat tccagttct 
      atggacttg aacgcgaat ggtgccatg aagaaagtc 
      aagtacgaa aatgagggc ggctctcta gactccctc 
      cacaaagag gtgtccata gatgacgat gcggatctg 
      gtgaccact ctgaccaac ctctacgac aagaaactg 
      atggagaag aagaactct cgggagaat ctcctctcc 
      ggggaggac ttcccctac gcggacgaa tcaccggag 
      aagacccac cgaatcatc gcgcattcc ctttcggac 
      aaagaaaac gtgccaaat ttaggcgac tgctaccga 
      aatcccagc ttacacaag aaccaattt agcaacaaa 
      gcgaatttt ttacactgg gacgactct aaacttaaa 
      gaatcgaaa aacgatacc gagaaagac gaagattcg 
      acaatgaca tccgagaag cacatatca aacaagcac 
      atgaacaac aggaaaaat tcggtgcca aactcgaaa 
      ctgcggcgt tcggagtcg ctgaacaag cccgaacgg 
      acagtctcg ccactgaac aacaagctg aagcggtcg 
      gaaagtctg aacaagacc ggggataaa ctcaagcgt 
      tcggacagt ctgaccaag acggaaaaa accgaaagc 
      aacttgaac aagcggcgg gagttggca acctccgtg 
      cgacgctcc aaggatgtg accaagctg aagaggaaa 
      aacggaatg ccggatcgg tcgatcaag agacggcac 
      actgttggc gggacgaag gatccagac aaagtcacg 
      tggctcgac aacaagtac caggaagaa gtgacttca 
      aacaaggaa aataaggag aagaacttg cggaccagc 
      tctccggac ttgagctcg acgaggcgc gagaggctg 
      ttcttcgaa ataaacctg atagggccc gagaacatg 
      gtcgtggcg ttgaagcag catttgatg ggcgctcgg 
      ccccagagt tttccggaa accgccgtt tttaaagta 
      ccgctcgaa tctcacgtt taa
    mRNA sequence
    • >TC034405 RA
      ccgcacttg aaaatctcg ttcgacctg ttataattt 
      cgctatcag cggtggtta tatttagca ccatccccg 
      atcgaaaga cgcgagctt cagttgtgc gggaatgaa 
      agcgagatc atgaacgtc ctcaccgaa cagcaccgg 
      aacaagtcc gggacggtc tcctgcatc cctcttcaa 
      ctcaacctg aatttctcc acacacgca tttttcccc 
      aggaaattg cgccgatgg gggtcgaca ggatcctgg 
      atttacgct ccgagtttt ttattacag ttatttaca 
      cgggtttat ttgataagt acacaaatt ttgacaaaa 
      ttaaacaga tctttgaac gaccttatc ttaaataaa 
      ttgattctt tatcaatga tgccaatat ttacataaa 
      aaatttttc gcgtagagc ccgatgggt acttccgac 
      gccgttgaa cattcacta gccagtggc gtggacatg 
      agggccagc gaagttcgc gtagctgat gattacacc 
      aaacgaaaa catgtcttg agggtatca tcagtgaat 
      ccttgtcgg tcggaattc ctcctccaa gccgaaaac 
      acagaagaa ttcactgac tggttcaag gctttgcaa 
      gaacaaatg gccacgaat accgaggcc gaagcgaaa 
      ttcgaccca tcgactggc agtaaacag caagccgta 
      ccacagacc atcccagcg tccacttcc atcctagtc 
      caaggcaac agccgcctc tcccctcag cccaccaaa 
      tgcaaatcc tccacctcc cgcaaccgt tccccaacc 
      ggtcaatcc cccgtgagc aaaaaccgc aaaccgtct 
      caagtggcc gacccctcc accagccct aaagtgaag 
      acatggcgg ggtcgcgtg gcccaacag ttccggcga 
      atgcaaggt gcaagttcg ccgagttcc ccgacggct 
      cccgaaggc tcaactttc gggattccc atcgaacag 
      tgtctcaca tcttcgacg aatccctac gttccgagg 
      ttcgtcgaa gtgtgtacg gacattgtc gacgctcac 
      ggattacaa acagtgggg atataccga gtgccggga 
      aataacgcc tcgattgta gcgttgatc gacgaaatc 
      aacaggaat tacgaagaa gtgcctgtg gatgatcca 
      cgatggaac gacctacac gttgttagt agccttctg 
      aaggccttc ttcaggaaa atccccgat tctttagtg 
      actagtgcg ttgtatccg agtttcatt aaggccgac 
      aaaatcgag aatcccgaa gccaggatg aaggaattg 
      aagaggctg attaagagt ttgccccct cacaattac 
      cacacgctt aaacacatc atgtttcac ttgaagaaa 
      gttatggat aattctgag attaataaa atggaagcg 
      aaaaattta gcgattgtc ttcggcccg aacattgtc 
      aggcccgag gacgacaat atggaaaca atggtcagt 
      aacatgacc catcagtat aaaatcgtg gaaactcta 
      ctgacaagc gccgattgg ttcttttcg gacgacgac 
      acggacgaa gtgcctctt tccgccgtt ataaccgaa 
      gcgcaggac gagccagaa cccccgagt agtcaagct 
      ctgttactc gaaaatatt agcaaatat gaagcttta 
      aaggataag caaaagaat ggcgcattg ctttcatcg 
      atcatttcg gcggcgcag cgaaaagtg aaaaggaaa 
      acgaccaaa ggttcaggt tccttaagc gaaagcaaa 
      gaagtcacg tctccgaac ggaaccgga ggctactat 
      tcggacaca ttctcacaa tctgatctc aaaaatagt 
      acaattacg gacgataaa agtaacgtc atacccaca 
      acagtgagc gaaatcgaa aagagttgc aaacagcct 
      gcaaaagtg ccttggttt aattactcg actgataaa 
      gaggatttt caccgacgt attgaaaac ttcaaacag 
      gagacggaa gctatgttg caaatgccg cgacatttg 
      gagatttcg gtgaccaac atcgcttcc aaaagtact 
      tcggctagt aacgtaaat caaacaccc cgactgtcc 
      acaagaacg gtcgactcc catttactt aaaacaaac 
      agtgcttcc aatgtattt acacgaact atcaacatg 
      gacaataga cacagtttg gacaattcg taccaagtg 
      caatacaat aaaaacaat agtgatttc ggtggcagt 
      ttacgcgac acgaacaga aacgagagt gttagtacg 
      caaaacggc cggttggtg cgacgtggc agttccgtc 
      gagaacgtc aattccagt tctatggac ttgaacgcg 
      aatggtgcc atgaagaaa gtcaagtac gaaaatgag 
      ggcggctct ctagactcc ctccacaaa gaggtgtcc 
      atagatgac gatgcggat ctggtgacc actctgacc 
      aacctctac gacaagaaa ctgatggag aagaagaac 
      tctcgggag aatctcctc tccggggag gacttcccc 
      tacgcggac gaatcaccg gagaagacc caccgaatc 
      atcgcgcat tccctttcg gacaaagaa aacgtgcca 
      aatttaggc gactgctac cgaaatccc agcttacac 
      aagaaccaa tttagcaac aaagcgaat tttttacac 
      tgggacgac tctaaactt aaagaatcg aaaaacgat 
      accgagaaa gacgaagat tcgacaatg acatccgag 
      aagcacata tcaaacaag cacatgaac aacaggaaa 
      aattcggtg ccaaactcg aaactgcgg cgttcggag 
      tcgctgaac aagcccgaa cggacagtc tcgccactg 
      aacaacaag ctgaagcgg tcggaaagt ctgaacaag 
      accggggat aaactcaag cgttcggac agtctgacc 
      aagacggaa aaaaccgaa agcaacttg aacaagcgg 
      cgggagttg gcaacctcc gtgcgacgc tccaaggat 
      gtgaccaag ctgaagagg aaaaacgga atgccggat 
      cggtcgatc aagagacgg cacactgtt ggcgggacg 
      aaggatcca gacaaagtc acgtggctc gacaacaag 
      taccaggaa gaagtgact tcaaacaag gaaaataag 
      gagaagaac ttgcggacc agctctccg gacttgagc 
      tcgacgagg cgcgagagg ctgttcttc gaaataaac 
      ctgataggg cccgagaac atggtcgtg gcgttgaag 
      cagcatttg atgggcgct cggccccag agttttccg 
      gaaaccgcc gtttttaaa gtaccgctc gaatctcac 
      gtttaagtc gtcgcgtgc cttaaaggg atagactga 
      gctgatgat gtgcaatac gcatgtgca ttgataatc 
      attatttat ttagggtaa attcataca tagaatgga 
      agttgcttt cgtttcgtg tgaaagtag gctaaggtt 
      ttgtttttt gatctttga aaaagacgg aaatgtaat 
      ttctaatat tatgacggc cgagttttt atttttaca 
      gcatttatt tgttgttac tttaagttt ttgctcctt 
      taaatgtat gtgattcta aaagtaatg aattaattc 
      aattccaag gtgaagcaa ttaaatttg tgataaaac 
      tgtagtgat ttaggcttg tacataact gtgttgttt 
      tactctcat gtttagttc tttatgctt tgattacca 
      cgtttttca tctttctat tatttcctt cttcaaaca 
      gtattattg aatactgtg ccaaaataa tatattatc 
      tagccactt tttgtaaat attgtttat ttgcttgtg 
      aattggtgt ataaaggtc ataggctga gcatattct 
      tgcctgtta ttgtttttt atagtttta agtacaaat 
      tgtatgtgt tgttgtatt ttgtgaaaa ttgtaaata 
      taattttca gtttatc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY