Status
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Gene information
General information
TC012583 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 11672160..11674230
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012583
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05275

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition 4 number 2nd lateral oviduct: number 1st lateral oviduct: blocked - Number of animals showing the phenotype: 1
    mature egg deposition 5 number 1st lateral oviduct: blocked - Number of animals showing the phenotype: 1
    mature egg deposition 2 number 2nd lateral oviduct: 3 number 1st lateral oviduct: blocked - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012583 RA
      MSGGSINKE NFEGTSGDQ SPTNEDSSL RLMMATAER 
      LAESTKRRH RDDNREHFR HERAEGYRE KRHSHRDRH 
      HRERRHRSI ERDRSRHHR KKSHRNERD TKGNLLGSK 
      YSDSCKYDV QKSKDFRNS NHDSENRKN ETENTVEES 
      KTFSEILED DFKDLESES PPSEKRLKL EEDSDESTE 
      SKLSEEIIE RPDEPDEKP EYDETLPPY YPAIEGCRS 
      LDEFQCLNR IAEGTYGVV YRFRDKRTD DIVALKKLK 
      MEKEKEGFP ITSLREINT LLKGQHPNI VTVREIVVG 
      SNMDKIFIV MDYVEHDLK SLIEMMRRK DQHFTPGEV 
      KCLLKQLLA AVAHLHDNW ILHRDLKTS NLLLSHNGI 
      LKVGDFGLA REYGSPLRP YTPIVVTLW YRAPELLLG 
      VKEYSTPID MWSVGCIFA EFLLMNAIF PGKSEAEQI 
      NRIFKCLGT PTEKIWPGF NKLPVAQKM KFTNHTVSN 
      LRKRFQMLN DLGLSFMLN FLTFDPHQR ITAEEALKH 
      PYFSEFPPP IDPSNFPKW PAKSELGQK RAAEASPKP 
      PSGGGEYKK LGDEDGFRI GLETDNSRI GAGFSLKF
    CDS sequence
    • >TC012583 RA
      atgtcgggc ggttcgatt aataaagaa aatttcgaa 
      ggcacctcc ggcgatcaa tcgcccaca aatgaggat 
      tcaagtttg agactgatg atggcgacg gccgaaagg 
      ctggccgag agtacaaaa cgacggcat cgggacgat 
      aaccgcgag cacttcagg cacgagagg gcggagggc 
      taccgggag aagagacac tcgcatagg gacaggcac 
      cacagagag cggcgccac cgctcaata gagcgggac 
      agatctcgc caccaccga aaaaaatca cacagaaat 
      gagagagat acaaaaggt aacttacta ggatcaaaa 
      tactctgat tcatgtaag tatgatgta cagaaatca 
      aaagatttt agaaattca aatcatgac tcagagaat 
      aggaaaaat gaaactgaa aatacagta gaagaatct 
      aaaacattt agcgagatt ttagaagac gatttcaaa 
      gatttagaa tcggaatcg ccgccttcg gagaaaagg 
      ctcaaactg gaggaagac agtgatgaa agtactgaa 
      agtaagctc agcgaggaa ataatagag agaccggac 
      gagcctgat gagaaaccc gagtatgac gaaacgctg 
      cctccctat taccctgcc attgagggc tgccggtca 
      ctggacgag ttccagtgt ttgaaccgt atcgctgag 
      ggcacctac ggcgtggtg tacaggttt cgggacaaa 
      agaactgac gacatagtc gccctcaaa aaactcaaa 
      atggagaag gagaaagaa ggctttccg attacgtcg 
      ctgcgcgaa atcaacacc ctcctcaag ggccagcac 
      ccaaatatc gtaacagtc cgagaaatc gtggtcggg 
      agcaatatg gataagatt tttatcgtg atggattac 
      gtcgagcac gatctcaaa tcgctgatc gagatgatg 
      aggcgcaaa gatcagcat tttacgccc ggagaagtc 
      aaatgtctc ctcaaacag ttgctagcg gcagtggca 
      catctccac gataattgg atcttgcac cgcgatttg 
      aaaacatca aatctgctt ctatcacat aatggtatt 
      ttgaaagtc ggcgatttt ggtttagcg agagagtat 
      ggatcaccg ctgcggcct tacactccg atagtggta 
      actctgtgg tacagggcg ccggagttg ttgctcggg 
      gtcaaagag tactcgacg ccgattgat atgtggtca 
      gtggggtgt atttttgcc gagttttta ctaatgaac 
      gcgattttt cccgggaaa tctgaggcc gagcagatc 
      aacaggatt tttaagtgt ttggggacg ccgactgag 
      aagatctgg cccggcttt aacaagttg ccggtggcc 
      caaaaaatg aaatttacg aaccacacg gtgtcgaat 
      ttgcgtaaa aggttccaa atgttgaac gatttgggt 
      ctgagcttc atgttgaac tttttgacc ttcgacccc 
      caccaaaga atcacagcc gaggaggct ttaaaacac 
      ccgtatttt agtgaattc ccgcctccg attgaccct 
      tcgaatttt cctaaatgg ccggcgaag agcgaactg 
      ggtcagaag cgggcggct gaggccagt ccgaagccg 
      ccatctggg ggcggcgag tataaaaaa ctaggtgac 
      gaagacggg tttaggata ggcctcgag actgacaat 
      agcaggata ggagccggg tttagttta aagttttaa
    mRNA sequence
    • >TC012583 RA
      gacgtttag tgtaattat tggtgcaaa agtgtggct 
      ttttgtaac gttttgtgt gttttctcg ttgtttgaa 
      gaatgttta gttaataat gtcgggcgg ttcgattaa 
      taaagaaaa tttcgaagg cacctccgg cgatcaatc 
      gcccacaaa tgaggattc aagtttgag actgatgat 
      ggcgacggc cgaaaggct ggccgagag tacaaaacg 
      acggcatcg ggacgataa ccgcgagca cttcaggca 
      cgagagggc ggagggcta ccgggagaa gagacactc 
      gcataggga caggcacca cagagagcg gcgccaccg 
      ctcaataga gcgggacag atctcgcca ccaccgaaa 
      aaaatcaca cagaaatga gagagatac aaaaggtaa 
      cttactagg atcaaaata ctctgattc atgtaagta 
      tgatgtaca gaaatcaaa agattttag aaattcaaa 
      tcatgactc agagaatag gaaaaatga aactgaaaa 
      tacagtaga agaatctaa aacatttag cgagatttt 
      agaagacga tttcaaaga tttagaatc ggaatcgcc 
      gccttcgga gaaaaggct caaactgga ggaagacag 
      tgatgaaag tactgaaag taagctcag cgaggaaat 
      aatagagag accggacga gcctgatga gaaacccga 
      gtatgacga aacgctgcc tccctatta ccctgccat 
      tgagggctg ccggtcact ggacgagtt ccagtgttt 
      gaaccgtat cgctgaggg cacctacgg cgtggtgta 
      caggtttcg ggacaaaag aactgacga catagtcgc 
      cctcaaaaa actcaaaat ggagaagga gaaagaagg 
      ctttccgat tacgtcgct gcgcgaaat caacaccct 
      cctcaaggg ccagcaccc aaatatcgt aacagtccg 
      agaaatcgt ggtcgggag caatatgga taagatttt 
      tatcgtgat ggattacgt cgagcacga tctcaaatc 
      gctgatcga gatgatgag gcgcaaaga tcagcattt 
      tacgcccgg agaagtcaa atgtctcct caaacagtt 
      gctagcggc agtggcaca tctccacga taattggat 
      cttgcaccg cgatttgaa aacatcaaa tctgcttct 
      atcacataa tggtatttt gaaagtcgg cgattttgg 
      tttagcgag agagtatgg atcaccgct gcggcctta 
      cactccgat agtggtaac tctgtggta cagggcgcc 
      ggagttgtt gctcggggt caaagagta ctcgacgcc 
      gattgatat gtggtcagt ggggtgtat ttttgccga 
      gtttttact aatgaacgc gatttttcc cgggaaatc 
      tgaggccga gcagatcaa caggatttt taagtgttt 
      ggggacgcc gactgagaa gatctggcc cggctttaa 
      caagttgcc ggtggccca aaaaatgaa atttacgaa 
      ccacacggt gtcgaattt gcgtaaaag gttccaaat 
      gttgaacga tttgggtct gagcttcat gttgaactt 
      tttgacctt cgaccccca ccaaagaat cacagccga 
      ggaggcttt aaaacaccc gtattttag tgaattccc 
      gcctccgat tgacccttc gaattttcc taaatggcc 
      ggcgaagag cgaactggg tcagaagcg ggcggctga 
      ggccagtcc gaagccgcc atctggggg cggcgagta 
      taaaaaact aggtgacga agacgggtt taggatagg 
      cctcgagac tgacaatag caggatagg agccgggtt 
      tagtttaaa gttttaaga gtaattttt taatgttcg 
      ctataaata aacttcagg tattttg
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