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Gene information
General information
TC012578 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 11651698..11654393
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012578
    iBeetle screen iB_05274

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012578 RA
      MIWSEYLGV FLYCLSQVS AEWNTKDFM KREHSLIKP 
      YYGSGIEIP FWHFTGSTI VTPNYIRLT SDLQSKVGA 
      IWNSVPVNV RNWELQVHF KVHGKGKDL FGDGFAIWY 
      AKDRMKEGP VFGNNDFFH GLAIILDTY SNHNGPHNH 
      QHPYISAMV NNGTLHYDH DRDGTHTQL AGCEAKFRN 
      LDYDTHIAI RYEKDVLTV STDIENKAA WKECFQVKG 
      VRLPTGYYL GVSATTGDL SDNHDIMSV RLFELDMPD 
      DPQADEDRS NIVPSATFF EPPRDHVED PKPSSLSGF 
      KVFILMLVG SIAVVACVV LGIMFYQKH HENKRKRFY
    CDS sequence
    • >TC012578 RA
      atgatttgg tccgagtat ttgggcgtt ttcctttac 
      tgtttgagt caagtgagt gcggaatgg aacacaaaa 
      gacttcatg aagcgagag cacagccta atcaagccg 
      tattacggc tctggcatc gagattccg ttctggcac 
      ttcaccggg agcacgata gtgaccccg aactacata 
      cgcctcacc tcggacctc cagagcaag gtcggggcg 
      atctggaac tccgtgccg gttaacgtg cgcaactgg 
      gagctgcaa gtccacttc aaagtgcac gggaagggc 
      aaggacctc ttcggggac gggttcgcg atctggtat 
      gccaaagac aggatgaag gagggcccg gtgtttggc 
      aataacgac tttttccac ggacttgcc atcatttta 
      gacacttat agcaaccac aatggacca cacaaccac 
      caacacccg tatatttcg gccatggtc aacaatggc 
      accctccac tatgaccat gacagggac gggacacat 
      acccagctc gcaggctgc gaagccaag ttcagaaac 
      ttggactac gacacgcac atagcgatc agatacgag 
      aaagatgta ctcacagtt tccaccgat attgaaaat 
      aaggcggcc tggaaggaa tgcttccaa gtcaagggg 
      gtgcgcctc ccgactggt tattactta ggtgtttct 
      gccacaacc ggggacttg tccgataat cacgatatt 
      atgtctgtt agattattt gagctggat atgcctgat 
      gatcctcag gccgatgag gacaggtcg aatattgtt 
      ccctcggct acgtttttc gagccgccg cgcgaccac 
      gtggaggac cccaagccc tcgtcgctc tccggtttc 
      aaagtcttt attttgatg ttggtgggg agcatagcc 
      gtggtggcg tgcgtcgtt ttggggatt atgttctat 
      cagaagcat cacgagaac aagaggaag aggttttat 
      taa
    mRNA sequence
    • >TC012578 RA
      ggcgggaac ggccatgtc ccaaagttg gattttcgg 
      ttaggctgt ttattaaac gtaaacaag ctttgggaa 
      gcttttgca tgaatcgtt cgtgtttca cgtgtttgg 
      tggtaaatt gaaagcaag atggcccgt tctaagaaa 
      accccaaac aagaagcct tcgtcagcc tctacgtcc 
      tattttatc ggaaacaaa gaatatcgc atcagactc 
      aagctgctc aggatgatt aagaagctg cagatgtct 
      acagagcta tgcatccca aaactatcg ttttcaaga 
      cttatccga gaacttctc caaagtcat agtcgaggg 
      acacgggat cttaggatt cagaaaagc gcgttgcaa 
      gcattgcac gaggcctcc gaggcctat ttgactgca 
      ctgttcgct gacagtaac ttgttggcg gcacatgca 
      caccgcgtt accataaaa ccgggggat atggcactc 
      tgcatgtac ataaggagg gaaaaagaa ggtggttaa 
      gtgttttac caagcgttg ctttaaacg ccagctgta 
      ctatgtatg ttttgtaac aagacatga ttatttcat 
      gttgagtcg ttgtcagtg taatcttta tgtgtgttt 
      ttgtattat tctagtttt aaaatttaa taaagacga 
      tattttcat aatcttaat gggtatttt ttgttacgt 
      tggttggca ctgcgacaa gatgccaag agtgcgata 
      cgtcaactg cacacaatt ccggtaaat tagcatttt 
      tatctctga aaaaatgat ttggtccga gtatttggg 
      cgttttcct ttactgttt gagtcaagt gagtgcgga 
      atggaacac aaaagactt catgaagcg agagcacag 
      cctaatcaa gccgtatta cggctctgg catcgagat 
      tccgttctg gcacttcac cgggagcac gatagtgac 
      cccgaacta catacgcct cacctcgga cctccagag 
      caaggtcgg ggcgatctg gaactccgt gccggttaa 
      cgtgcgcaa ctgggagct gcaagtcca cttcaaagt 
      gcacgggaa gggcaagga cctcttcgg ggacgggtt 
      cgcgatctg gtatgccaa agacaggat gaaggaggg 
      cccggtgtt tggcaataa cgacttttt ccacggact 
      tgccatcat tttagacac ttatagcaa ccacaatgg 
      accacacaa ccaccaaca cccgtatat ttcggccat 
      ggtcaacaa tggcaccct ccactatga ccatgacag 
      ggacgggac acataccca gctcgcagg ctgcgaagc 
      caagttcag aaacttgga ctacgacac gcacatagc 
      gatcagata cgagaaaga tgtactcac agtttccac 
      cgatattga aaataaggc ggcctggaa ggaatgctt 
      ccaagtcaa gggggtgcg cctcccgac tggttatta 
      cttaggtgt ttctgccac aaccgggga cttgtccga 
      taatcacga tattatgtc tgttagatt atttgagct 
      ggatatgcc tgatgatcc tcaggccga tgaggacag 
      gtcgaatat tgttccctc ggctacgtt tttcgagcc 
      gccgcgcga ccacgtgga ggaccccaa gccctcgtc 
      gctctccgg tttcaaagt ctttatttt gatgttggt 
      ggggagcat agccgtggt ggcgtgcgt cgttttggg 
      gattatgtt ctatcagaa gcatcacga gaacaagag 
      gaagaggtt ttattaatc tcgtttgaa gggttattg 
      tattactgt aattatttg actgatttt tttcacggt 
      aggtaattg ggtgccgaa ataatagtc gatatttat 
      taaattgtg ttggtgtaa atatgtcat ttttcgaaa 
      tgagagtgt acaaaacca ttcagatgt ttatatttt 
      ttgtttata ttcttacaa cagtttaca acattaatt 
      taagaaaac gtacgtatt caaacacat aatgaactt 
      aactgtaac gaaaaaaat gactacaat gactgacta 
      aaacgagaa atgtgccag gaatctggc acatgcttt 
      ccaactatt tcaggtttt tacatttgg ttccgattt 
      gttcaaaat tttatacct ataaaacta tatataaaa 
      gaaaatgtg atttataaa caaaaattt aaataaatc 
      ttttttaag tatttgtga ctagtacat ttatgtttg 
      tttttaagg gctcgcgat ataagtaca tttaccggt 
      caatattac aacatataa tttttatta cagcttgtt 
      acactcagc cacagcttc tttaacaat gaggtcctc 
      agttcctta tcttttttg tttccaggg tcagtaact 
      tcagggtga gccttcaag ctaaggaat tagagcttt 
      tggcaaata aatcggaat tttttat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY