Status
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Gene information
General information
TC012567 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 11537719..11547481
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012567
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05270

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012567 RA
      MDCNEFRIK GKEMVDYIC NYNENLHSR RVTPNIEPG 
      YLRKLLPEE APHNPEDWD AIMSDIESK IMPGVTHWQ 
      HPRFHAYFP SGNSFPSIL GDMLSDGIG CIGFSWAAS 
      PALTELETI VLDWLGKAI GLPDQFLAF KEGSRGGGV 
      IQTSASECV LVSMLAARA QALKRLKQQ HPFVEEGLL 
      LSKLMAYCS KEAHSCVEK AAMICFVKL RILEPDEKS 
      SLRGSTLMM AMEEDETMG LIPFFVSTT LGTTSCCSF 
      DNLNEIGPV CRKFPCVWL HVDGAYAGN AFICPELKY 
      LLSGVEYAD SFNTNPNKW LLTNFDCST MWVRDRIRL 
      TSALVVDPL YLQHGYSDA TIDYRHWGV PLSRRFRSL 
      KLWFVLRSY GISGLQKYI RHHIKLAKI FESLVLKDH 
      RFEICNNVK LGLVCFRLK GSDKINEKL LCNINASGK 
      LHMVPANVN DKYVIRFCV VAQNATEAD IDYAWKVIK 
      DFSEELFDS QALEKEQEQ QDEVFELLE RKKKETLAQ 
      KRSFFVRMV SDPKIYNPS IAKALPSTR RHLTETAES 
      PDGNVTVQP PLSASWISW PLAFLFQGI NEDGTSSDV 
      PIRFRHLDT KVRLKAAGK ATNGGQSPS PEGENSKSP 
      KRSPVFPRK LHQTDH
    CDS sequence
    • >TC012567 RA
      atggactgt aacgaattt cgaataaaa ggcaaagaa 
      atggtggac tacatctgc aactataac gagaacctc 
      cactcccgg cgcgtcacc cccaacatc gagccgggc 
      tacctccgc aaactcctc cccgaagaa gccccccac 
      aaccccgaa gactgggac gccatcatg tccgacatc 
      gagtcaaaa ataatgcct ggagtgact cactggcaa 
      catccgcgc ttccacgcg tatttcccc tccggaaat 
      tcctttccc tccattctt ggagacatg ctctcagac 
      gggatcggc tgtatcggc ttctcctgg gctgcaagt 
      ccggctttg accgaactt gaaaccatc gttttggac 
      tggttgggg aaggcgatc ggtttgccg gatcagttt 
      ttggcgttt aaggaagga agtcgaggt ggaggagtt 
      atccagacg tcggccagc gaatgcgtg ctggtgtcg 
      atgttggcg gcgagggcg caggctttg aagaggttg 
      aagcagcaa catccgttc gtggaggag gggcttttg 
      ctgtcgaag ttgatggcg tattgctcc aaggaggcg 
      cactcgtgt gtggagaag gcagcgatg atttgtttc 
      gtcaagttg aggattttg gaaccggat gaaaaatcc 
      tcactgagg gggtccaca cttatgatg gcaatggaa 
      gaggacgag accatgggc ctcatcccg ttcttcgtg 
      tccacaacc ctgggcacc acctcgtgc tgctccttc 
      gacaacctg aacgagatc ggccccgtg tgccgcaaa 
      ttcccctgc gtgtggctg cacgtggac ggagcttac 
      gccggcaat gccttcatt tgccccgag ctgaaatac 
      ctcctgtcc ggggtcgaa tacgccgac tctttcaac 
      acgaacccg aacaaatgg ttattgaca aatttcgat 
      tgttccaca atgtgggtg cgagacaga attcgatta 
      acatccgca ttggtggta gatccgctt tatctccaa 
      cacggatat tcagacgcc acgattgat taccgccac 
      tggggagtc cccttaagt cggagattt agatcgctg 
      aagctgtgg tttgtacta agaagttac gggatttcc 
      ggactacaa aaatacatc aggcatcac attaagctt 
      gccaagatt ttcgaatcg ttggttctc aaagatcac 
      agatttgaa atttgcaat aatgtcaaa ctgggtctt 
      gtgtgtttc cggttgaag ggatctgat aaaattaat 
      gaaaagctg ttgtgcaac atcaatgct tccgggaaa 
      cttcacatg gttccggcc aatgttaat gataaatat 
      gttatcagg ttctgtgtg gtggcacaa aatgccacc 
      gaggccgat atagattat gcatggaag gttattaag 
      gacttttca gaagaatta ttcgatagt caggcttta 
      gaaaaagaa caagagcag caagacgag gtgttcgag 
      ctgctggag cgcaagaaa aaggagaca ttggcccag 
      aaacgctcc ttcttcgtg cgtatggtc agtgatccg 
      aagatctac aatccttcg atcgccaaa gctttgccc 
      tcaactcgg agacatctg accgagacc gccgagtca 
      cccgatggg aatgtcacg gtccaaccg cctttgagc 
      gcttcgtgg atcagctgg ccgttggcc ttcctcttc 
      caaggaatc aacgaggat ggaacctcg agcgacgtc 
      ccgattcgg ttccggcat ctcgatacg aaagttcga 
      ttaaaagcc gctggaaaa gcaacaaac gggggccag 
      tcgccctcg cccgagggg gaaaacagc aagtcgccg 
      aaacgttcg ccggttttc cccagaaaa ctgcatcaa 
      actgatcac taa
    mRNA sequence
    • >TC012567 RA
      cagcatttc ttccccgta tgcaatttt acgagctca 
      attaatttt cagtttgac catttttgt tctaaactt 
      gagctattt ccattgttg tgaaaatgg gattcttta 
      tgagtcgat gtgattgga gtggaattc ctgcacgtt 
      tgacgtcgc gtcggtgcg aaaagtcaa ttagatgga 
      gaagctctt ttgtagtcg gggatgcaa aattattgg 
      ggaaagaag tcgtaggtc gaagggtaa aattatttg 
      cagagttaa acggtggca gttggtggt gaaactcgt 
      gccggagaa tcaggaagt ggtacgaag caacagcca 
      tggactgta acgaatttc gaataaaag gcaaagaaa 
      tggtggact acatctgca actataacg agaacctcc 
      actcccggc gcgtcaccc ccaacatcg agccgggct 
      acctccgca aactcctcc ccgaagaag ccccccaca 
      accccgaag actgggacg ccatcatgt ccgacatcg 
      agtcaaaaa taatgcctg gagtgactc actggcaac 
      atccgcgct tccacgcgt atttcccct ccggaaatt 
      cctttccct ccattcttg gagacatgc tctcagacg 
      ggatcggct gtatcggct tctcctggg ctgcaagtc 
      cggctttga ccgaacttg aaaccatcg ttttggact 
      ggttgggga aggcgatcg gtttgccgg atcagtttt 
      tggcgttta aggaaggaa gtcgaggtg gaggagtta 
      tccagacgt cggccagcg aatgcgtgc tggtgtcga 
      tgttggcgg cgagggcgc aggctttga agaggttga 
      agcagcaac atccgttcg tggaggagg ggcttttgc 
      tgtcgaagt tgatggcgt attgctcca aggaggcgc 
      actcgtgtg tggagaagg cagcgatga tttgtttcg 
      tcaagttga ggattttgg aaccggatg aaaaatcct 
      cactgaggg ggtccacac ttatgatgg caatggaag 
      aggacgaga ccatgggcc tcatcccgt tcttcgtgt 
      ccacaaccc tgggcacca cctcgtgct gctccttcg 
      acaacctga acgagatcg gccccgtgt gccgcaaat 
      tcccctgcg tgtggctgc acgtggacg gagcttacg 
      ccggcaatg ccttcattt gccccgagc tgaaatacc 
      tcctgtccg gggtcgaat acgccgact ctttcaaca 
      cgaacccga acaaatggt tattgacaa atttcgatt 
      gttccacaa tgtgggtgc gagacagaa ttcgattaa 
      catccgcat tggtggtag atccgcttt atctccaac 
      acggatatt cagacgcca cgattgatt accgccact 
      ggggagtcc ccttaagtc ggagattta gatcgctga 
      agctgtggt ttgtactaa gaagttacg ggatttccg 
      gactacaaa aatacatca ggcatcaca ttaagcttg 
      ccaagattt tcgaatcgt tggttctca aagatcaca 
      gatttgaaa tttgcaata atgtcaaac tgggtcttg 
      tgtgtttcc ggttgaagg gatctgata aaattaatg 
      aaaagctgt tgtgcaaca tcaatgctt ccgggaaac 
      ttcacatgg ttccggcca atgttaatg ataaatatg 
      ttatcaggt tctgtgtgg tggcacaaa atgccaccg 
      aggccgata tagattatg catggaagg ttattaagg 
      acttttcag aagaattat tcgatagtc aggctttag 
      aaaaagaac aagagcagc aagacgagg tgttcgagc 
      tgctggagc gcaagaaaa aggagacat tggcccaga 
      aacgctcct tcttcgtgc gtatggtca gtgatccga 
      agatctaca atccttcga tcgccaaag ctttgccct 
      caactcgga gacatctga ccgagaccg ccgagtcac 
      ccgatggga atgtcacgg tccaaccgc ctttgagcg 
      cttcgtgga tcagctggc cgttggcct tcctcttcc 
      aaggaatca acgaggatg gaacctcga gcgacgtcc 
      cgattcggt tccggcatc tcgatacga aagttcgat 
      taaaagccg ctggaaaag caacaaacg ggggccagt 
      cgccctcgc ccgaggggg aaaacagca agtcgccga 
      aacgttcgc cggttttcc ccagaaaac tgcatcaaa 
      ctgatcact aaatgtacc gcttaatta caataaatt 
      aggcttgca aagtaataa agtcgtttc atgtct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY