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Gene information
General information
TC012561 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 11496359..11497796
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012561
    iBeetle screen iB_05268

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012561 RA
      MLRRQARLR REYLYRKTV EDKQKSIQD KKDRIKRSL 
      EENIPIHGD LQKKALHYS KKLEWEDAG PAAAVQLGG 
      ESGGALASS IDDEYRYAG VEDPKIVIT TSRDPSSRL 
      KMFVKELRL IFPNAQRMN RGNYEMKQL IHACRANDV 
      TDFIVVHEH RGVPDSLVV CHLPYGPTA YFTMSDVVM 
      RHDIPDLGT MSEQYPHLI FHNFKTKLG ERVTNILKY 
      LFPVPKEDS KRIITFANH DDYISFRHH NYKTVDKQI 
      ELSEVGPRF QLRLYEIKL GTLDNADAA DTEWALRPY 
      MNTTVKRRF LSDEDGWLQ EDTMIS
    CDS sequence
    • >TC012561 RA
      atgttacgc cgccaagct cgcttacga cgcgagtac 
      ctctaccgg aaaaccgtc gaagacaag caaaaatcc 
      atccaagac aagaaggac cgcatcaag cgcagttta 
      gaggagaac atccccatc catggggac ctccagaag 
      aaggccctg cactactcc aagaagctc gagtgggag 
      gacgcgggg cccgcggcc gccgtccag ctcggcggc 
      gagagcggc ggggcccta gccagctcc atcgacgac 
      gagtacaga tacgcgggg gtcgaggac cccaaaatt 
      gtcatcacg acgagtcgg gaccccagc tcgagactc 
      aaaatgttc gtcaaggaa ctgcgcttg attttcccc 
      aacgcccag cggatgaat cggggcaac tacgagatg 
      aagcagctg attcatgcg tgcagggcc aatgacgtg 
      accgatttc atcgtagtg catgagcac aggggggtg 
      ccggacagt ttagtcgtt tgccatctg ccctacggc 
      cccaccgcc tattttacg atgtccgat gttgtcatg 
      aggcatgac atccccgat ttggggacc atgtccgag 
      caatacccc catctgatt ttccataat tttaaaacg 
      aaacttggg gagcgtgtc acgaacatt ttgaagtac 
      ttgtttcca gtgcccaag gaggactcc aaacggata 
      atcaccttc gccaaccac gacgattat attagcttt 
      aggcaccac aattataaa acagtcgat aagcagatc 
      gaattgtct gaagtgggg ccccgattc cagctgcgc 
      ctctatgag atcaagtta gggacttta gacaatgcg 
      gacgctgcc gatacagag tgggcttta aggccctac 
      atgaacaca acggtgaaa cggagattt ttaagtgac 
      gaagacggt tggcttcaa gaggacaca atgatctca 
      taa
    mRNA sequence
    • >TC012561 RA
      aaacacagg aataacatt tcgtgttag cagttttca 
      attattttg ggctccaga caattaaat aagtgacga 
      aaaaatgat agtttaacg cgttagcgc tttgtgata 
      cgcaatgga aaattttca agccaagaa gccaaaatc 
      ggaagaaaa tgttacgcc gccaagctc gcttacgac 
      gcgagtacc tctaccgga aaaccgtcg aagacaagc 
      aaaaatcca tccaagaca agaaggacc gcatcaagc 
      gcagtttag aggagaaca tccccatcc atggggacc 
      tccagaaga aggccctgc actactcca agaagctcg 
      agtgggagg acgcggggc ccgcggccg ccgtccagc 
      tcggcggcg agagcggcg gggccctag ccagctcca 
      tcgacgacg agtacagat acgcggggg tcgaggacc 
      ccaaaattg tcatcacga cgagtcggg accccagct 
      cgagactca aaatgttcg tcaaggaac tgcgcttga 
      ttttcccca acgcccagc ggatgaatc ggggcaact 
      acgagatga agcagctga ttcatgcgt gcagggcca 
      atgacgtga ccgatttca tcgtagtgc atgagcaca 
      ggggggtgc cggacagtt tagtcgttt gccatctgc 
      cctacggcc ccaccgcct attttacga tgtccgatg 
      ttgtcatga ggcatgaca tccccgatt tggggacca 
      tgtccgagc aataccccc atctgattt tccataatt 
      ttaaaacga aacttgggg agcgtgtca cgaacattt 
      tgaagtact tgtttccag tgcccaagg aggactcca 
      aacggataa tcaccttcg ccaaccacg acgattata 
      ttagcttta ggcaccaca attataaaa cagtcgata 
      agcagatcg aattgtctg aagtggggc cccgattcc 
      agctgcgcc tctatgaga tcaagttag ggactttag 
      acaatgcgg acgctgccg atacagagt gggctttaa 
      ggccctaca tgaacacaa cggtgaaac ggagatttt 
      taagtgacg aagacggtt ggcttcaag aggacacaa 
      tgatctcat aacttttat ttacttttt taataaatt 
      atctgacga cga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY