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Gene information
General information
TC012532 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 11037415..11042919
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012532
    iBeetle screen iB_05261

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    larva appendages shortened - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs pretarsus partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012532 RA
      MSAVMDMYL SASDSMSFQ DILDIDIKS EIESLVGGH 
      NDFSGLNFS ELPPLELED VSDIKWFSS NSNHSNSSL 
      NLDFNGEER TTMVNPNAV MPAISLAQS IRSPSPTLK 
      ESHLTFSPS TIKITTMKQ EANQVKKDL LKRLSDDDR 
      EDKPPVLKT ISKVTPIIS KPPARTISK PTLLTFRNT 
      INKQVNSPI TVAQVSHVT TVKTVTSTN RKLNNNRFS 
      EDGERIYPK PAYSYSCLI AMALKNSRS GSLPVSEIY 
      NFMCKHFPY FITAPNGWK NSVRHNLSL NKCFEKIEK 
      PAINGAQRK GCLWAMNPA KISKMDEEV QKWSRKDPS 
      AIRKAMINP ENLEPLERG ELKFSCSYD EGDTFGDSC 
      GSAESEEGS DYEEGPKRD FEFVIKAEN EDGSDFDIE 
      VNSDLDVEA SDEVYEDIE EDQQMLRVE MALAQKELM 
      EYEKPVNSK RRRTYIVQN
    CDS sequence
    • >TC012532 RA
      atgtctgcc gtcatggac atgtacttg agcgcatcc 
      gattcaatg tcatttcaa gacatcttg gacatcgac 
      attaaatcc gaaatagaa tcgttggtc ggcggtcat 
      aacgacttt tccggcttg aatttctcg gaattgcct 
      ccactggaa ttggaagac gtttccgac ataaaatgg 
      ttttcgtca aactcgaac cattcaaat tccagctta 
      aatttagat tttaacggc gaagaacgg accacaatg 
      gtcaatccg aacgccgta atgcctgct atttcctta 
      gcccaaagt attcgttcg ccgtcaccg acgttaaaa 
      gaaagtcac ttaacgttc tctccatcc actattaaa 
      ataacaaca atgaaacaa gaagcgaat caagtgaag 
      aaagatctt ttaaaacgg ctgagtgac gacgacagg 
      gaagacaaa ccccctgta ttgaaaaca ataagtaaa 
      gtcactcca atcatctca aaacctcca gctaggacc 
      ataagcaaa ccgacgttg ttaactttt agaaataca 
      attaataaa caagttaat tcccctatt acggtagct 
      caggttagt cacgtcacc acagttaag acggtaact 
      tccactaac cgaaaacta aacaataac cgattcagc 
      gaagacggc gaacggatt tacccgaaa ccggcatac 
      agttacagt tgtctcatc gcaatggcg ctgaaaaac 
      agccgatcg ggtagttta cccgtctca gaaatctac 
      aatttcatg tgtaaacac tttccgtac ttcataacg 
      gcacctaac ggctggaaa aactcagtc agacacaat 
      ctttcgctg aataaatgc ttcgaaaag atagagaaa 
      ccggcgatc aacggggcg caaaggaaa ggctgtttg 
      tgggcgatg aatcccgca aagatcagc aaaatggac 
      gaggaagtc cagaaatgg tcccggaag gacccttca 
      gccattagg aaggctatg atcaatcct gaaaatttg 
      gaaccgctg gaaaggggc gagttgaag ttctcgtgc 
      agttacgac gaaggggac acgtttggg gactcctgc 
      gggagtgcc gaaagtgaa gaagggtcg gattatgag 
      gaagggccg aaaagggac ttcgagttc gtgatcaag 
      gcggagaac gaggacggc agcgacttc gatatcgaa 
      gtcaatagt gatcttgat gttgaggct tccgacgag 
      gtgtacgag gacatagag gaggatcag cagatgtta 
      cgagtggag atggcgctc gctcagaag gagctaatg 
      gagtacgag aagcccgtc aacagcaag agacggagg 
      acgtacata gtccagaat taa
    mRNA sequence
    • >TC012532 RA
      aggcgcatc aaaataatc tgcgaaaaa cgggcgaaa 
      ttttggtgt gcgccattt cccgctttt ccgttcctc 
      cagcgaaaa aacttaggt taaattaaa tgtcaagac 
      cccattagc acgcgtatc gttattcga ttgcgaagt 
      cggcgttcc ggcgactct cgccggcca atcagaatc 
      gcgcgggaa acactcgtg acttgcgcc agccaatcg 
      ggagcgcgc gatttgaat tccctcttc gttctttgt 
      ggatgacga caagttttg tatacattc gttgggtgt 
      aaagtgctg tcagtgttg aatatcttt ttatccatg 
      atttaaaca aaaatacga ccagacaca acgaactgc 
      cgtcaaata acgcatcaa agctaatcg tcatgtctg 
      ccgtcatgg acatgtact tgagcgcat ccgattcaa 
      tgtcatttc aagacatct tggacatcg acattaaat 
      ccgaaatag aatcgttgg tcggcggtc ataacgact 
      tttccggct tgaatttct cggaattgc ctccactgg 
      aattggaag acgtttccg acataaaat ggttttcgt 
      caaactcga accattcaa attccagct taaatttag 
      attttaacg gcgaagaac ggaccacaa tggtcaatc 
      cgaacgccg taatgcctg ctatttcct tagcccaaa 
      gtattcgtt cgccgtcac cgacgttaa aagaaagtc 
      acttaacgt tctctccat ccactatta aaataacaa 
      caatgaaac aagaagcga atcaagtga agaaagatc 
      ttttaaaac ggctgagtg acgacgaca gggaagaca 
      aaccccctg tattgaaaa caataagta aagtcactc 
      caatcatct caaaacctc cagctagga ccataagca 
      aaccgacgt tgttaactt ttagaaata caattaata 
      aacaagtta attccccta ttacggtag ctcaggtta 
      gtcacgtca ccacagtta agacggtaa cttccacta 
      accgaaaac taaacaata accgattca gcgaagacg 
      gcgaacgga tttacccga aaccggcat acagttaca 
      gttgtctca tcgcaatgg cgctgaaaa acagccgat 
      cgggtagtt tacccgtct cagaaatct acaatttca 
      tgtgtaaac actttccgt acttcataa cggcaccta 
      acggctgga aaaactcag tcagacaca atctttcgc 
      tgaataaat gcttcgaaa agatagaga aaccggcga 
      tcaacgggg cgcaaagga aaggctgtt tgtgggcga 
      tgaatcccg caaagatca gcaaaatgg acgaggaag 
      tccagaaat ggtcccgga aggaccctt cagccatta 
      ggaaggcta tgatcaatc ctgaaaatt tggaaccgc 
      tggaaaggg gcgagttga agttctcgt gcagttacg 
      acgaagggg acacgtttg gggactcct gcgggagtg 
      ccgaaagtg aagaagggt cggattatg aggaagggc 
      cgaaaaggg acttcgagt tcgtgatca aggcggaga 
      acgaggacg gcagcgact tcgatatcg aagtcaata 
      gtgatcttg atgttgagg cttccgacg aggtgtacg 
      aggacatag aggaggatc agcagatgt tacgagtgg 
      agatggcgc tcgctcaga aggagctaa tggagtacg 
      agaagcccg tcaacagca agagacgga ggacgtaca 
      tagtccaga attaagttg gtatttgta taaacagat 
      cgtgtcggt gtccatctg gtcacacat ctccataag 
      taaagtgaa gctgtgtaa gattggtga taattgtaa 
      ataattgta actttcctg cccgtatat ttcttaaga 
      ttagttgat tcattgttt tatacttga agactgccc 
      tttcgattt tctttgtta aaatcaact agttttatg 
      ttttatgaa acaatgaat tgagagaac agttttgtt 
      gtattttgt aaaatttcg tcatttgcc aagacagta 
      tttggaagt agatgtgga ctgatggca agagcaaaa 
      ttgatcggt ccaaatgtg aatgtcgcc attcacatt 
      cggaccgct tttgtgttt gtttacata acgtgcctt 
      tgctccaaa ctcaatttt aggtctttg tatttcctc 
      ttggtggga ccagttcac agaatttta tttgctctt 
      attttattc gccacttaa tgtatcatg tgatagttt 
      tctcatcca attcttgtt aatttattg tgtttccga 
      ctgcgttaa gaaacgcgt ttcttaatg cactcggaa 
      taatttact aatcatcag tagtctcga tcgtcaatg 
      taggtagaa ttttttaat tttttaaga gcaaaagag 
      aacagactt tttttatta aaaaattcc tagctttgc 
      gttgaagct tttgtctaa aattttgta ctatataat 
      tgatttttg tattttttt aattttaat tttaagtga 
      cttgttata agctgatgt acaaatttt gttagtctt 
      aataaatcc ttgtaaaaa taaaatcgt cactttcga 
      atgattgtt ttgattcct accacgaac ggtttgagc 
      cccttagct tatttttac aagagatta agtcataat 
      acgcatagt tgaatagca atgtaggcc attaatttc 
      cgaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY