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Gene information
General information
TC012532 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 11037415..11042919
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC012532
    iBeetle screen iB_05261

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     larva appendages shortened - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs pretarsus partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012532 RA
      MSAVMDMYL SASDSMSFQ DILDIDIKS EIESLVGGH 
NDFSGLNFS ELPPLELED VSDIKWFSS NSNHSNSSL 
NLDFNGEER TTMVNPNAV MPAISLAQS IRSPSPTLK 
ESHLTFSPS TIKITTMKQ EANQVKKDL LKRLSDDDR 
EDKPPVLKT ISKVTPIIS KPPARTISK PTLLTFRNT 
INKQVNSPI TVAQVSHVT TVKTVTSTN RKLNNNRFS 
EDGERIYPK PAYSYSCLI AMALKNSRS GSLPVSEIY 
NFMCKHFPY FITAPNGWK NSVRHNLSL NKCFEKIEK 
PAINGAQRK GCLWAMNPA KISKMDEEV QKWSRKDPS 
AIRKAMINP ENLEPLERG ELKFSCSYD EGDTFGDSC 
GSAESEEGS DYEEGPKRD FEFVIKAEN EDGSDFDIE 
VNSDLDVEA SDEVYEDIE EDQQMLRVE MALAQKELM 
EYEKPVNSK RRRTYIVQN
    CDS sequence
    • >TC012532 RA
      atgtctgcc gtcatggac atgtacttg agcgcatcc 
gattcaatg tcatttcaa gacatcttg gacatcgac 
attaaatcc gaaatagaa tcgttggtc ggcggtcat 
aacgacttt tccggcttg aatttctcg gaattgcct 
ccactggaa ttggaagac gtttccgac ataaaatgg 
ttttcgtca aactcgaac cattcaaat tccagctta 
aatttagat tttaacggc gaagaacgg accacaatg 
gtcaatccg aacgccgta atgcctgct atttcctta 
gcccaaagt attcgttcg ccgtcaccg acgttaaaa 
gaaagtcac ttaacgttc tctccatcc actattaaa 
ataacaaca atgaaacaa gaagcgaat caagtgaag 
aaagatctt ttaaaacgg ctgagtgac gacgacagg 
gaagacaaa ccccctgta ttgaaaaca ataagtaaa 
gtcactcca atcatctca aaacctcca gctaggacc 
ataagcaaa ccgacgttg ttaactttt agaaataca 
attaataaa caagttaat tcccctatt acggtagct 
caggttagt cacgtcacc acagttaag acggtaact 
tccactaac cgaaaacta aacaataac cgattcagc 
gaagacggc gaacggatt tacccgaaa ccggcatac 
agttacagt tgtctcatc gcaatggcg ctgaaaaac 
agccgatcg ggtagttta cccgtctca gaaatctac 
aatttcatg tgtaaacac tttccgtac ttcataacg 
gcacctaac ggctggaaa aactcagtc agacacaat 
ctttcgctg aataaatgc ttcgaaaag atagagaaa 
ccggcgatc aacggggcg caaaggaaa ggctgtttg 
tgggcgatg aatcccgca aagatcagc aaaatggac 
gaggaagtc cagaaatgg tcccggaag gacccttca 
gccattagg aaggctatg atcaatcct gaaaatttg 
gaaccgctg gaaaggggc gagttgaag ttctcgtgc 
agttacgac gaaggggac acgtttggg gactcctgc 
gggagtgcc gaaagtgaa gaagggtcg gattatgag 
gaagggccg aaaagggac ttcgagttc gtgatcaag 
gcggagaac gaggacggc agcgacttc gatatcgaa 
gtcaatagt gatcttgat gttgaggct tccgacgag 
gtgtacgag gacatagag gaggatcag cagatgtta 
cgagtggag atggcgctc gctcagaag gagctaatg 
gagtacgag aagcccgtc aacagcaag agacggagg 
acgtacata gtccagaat taa
    mRNA sequence
    • >TC012532 RA
      aggcgcatc aaaataatc tgcgaaaaa cgggcgaaa 
ttttggtgt gcgccattt cccgctttt ccgttcctc 
cagcgaaaa aacttaggt taaattaaa tgtcaagac 
cccattagc acgcgtatc gttattcga ttgcgaagt 
cggcgttcc ggcgactct cgccggcca atcagaatc 
gcgcgggaa acactcgtg acttgcgcc agccaatcg 
ggagcgcgc gatttgaat tccctcttc gttctttgt 
ggatgacga caagttttg tatacattc gttgggtgt 
aaagtgctg tcagtgttg aatatcttt ttatccatg 
atttaaaca aaaatacga ccagacaca acgaactgc 
cgtcaaata acgcatcaa agctaatcg tcatgtctg 
ccgtcatgg acatgtact tgagcgcat ccgattcaa 
tgtcatttc aagacatct tggacatcg acattaaat 
ccgaaatag aatcgttgg tcggcggtc ataacgact 
tttccggct tgaatttct cggaattgc ctccactgg 
aattggaag acgtttccg acataaaat ggttttcgt 
caaactcga accattcaa attccagct taaatttag 
attttaacg gcgaagaac ggaccacaa tggtcaatc 
cgaacgccg taatgcctg ctatttcct tagcccaaa 
gtattcgtt cgccgtcac cgacgttaa aagaaagtc 
acttaacgt tctctccat ccactatta aaataacaa 
caatgaaac aagaagcga atcaagtga agaaagatc 
ttttaaaac ggctgagtg acgacgaca gggaagaca 
aaccccctg tattgaaaa caataagta aagtcactc 
caatcatct caaaacctc cagctagga ccataagca 
aaccgacgt tgttaactt ttagaaata caattaata 
aacaagtta attccccta ttacggtag ctcaggtta 
gtcacgtca ccacagtta agacggtaa cttccacta 
accgaaaac taaacaata accgattca gcgaagacg 
gcgaacgga tttacccga aaccggcat acagttaca 
gttgtctca tcgcaatgg cgctgaaaa acagccgat 
cgggtagtt tacccgtct cagaaatct acaatttca 
tgtgtaaac actttccgt acttcataa cggcaccta 
acggctgga aaaactcag tcagacaca atctttcgc 
tgaataaat gcttcgaaa agatagaga aaccggcga 
tcaacgggg cgcaaagga aaggctgtt tgtgggcga 
tgaatcccg caaagatca gcaaaatgg acgaggaag 
tccagaaat ggtcccgga aggaccctt cagccatta 
ggaaggcta tgatcaatc ctgaaaatt tggaaccgc 
tggaaaggg gcgagttga agttctcgt gcagttacg 
acgaagggg acacgtttg gggactcct gcgggagtg 
ccgaaagtg aagaagggt cggattatg aggaagggc 
cgaaaaggg acttcgagt tcgtgatca aggcggaga 
acgaggacg gcagcgact tcgatatcg aagtcaata 
gtgatcttg atgttgagg cttccgacg aggtgtacg 
aggacatag aggaggatc agcagatgt tacgagtgg 
agatggcgc tcgctcaga aggagctaa tggagtacg 
agaagcccg tcaacagca agagacgga ggacgtaca 
tagtccaga attaagttg gtatttgta taaacagat 
cgtgtcggt gtccatctg gtcacacat ctccataag 
taaagtgaa gctgtgtaa gattggtga taattgtaa 
ataattgta actttcctg cccgtatat ttcttaaga 
ttagttgat tcattgttt tatacttga agactgccc 
tttcgattt tctttgtta aaatcaact agttttatg 
ttttatgaa acaatgaat tgagagaac agttttgtt 
gtattttgt aaaatttcg tcatttgcc aagacagta 
tttggaagt agatgtgga ctgatggca agagcaaaa 
ttgatcggt ccaaatgtg aatgtcgcc attcacatt 
cggaccgct tttgtgttt gtttacata acgtgcctt 
tgctccaaa ctcaatttt aggtctttg tatttcctc 
ttggtggga ccagttcac agaatttta tttgctctt 
attttattc gccacttaa tgtatcatg tgatagttt 
tctcatcca attcttgtt aatttattg tgtttccga 
ctgcgttaa gaaacgcgt ttcttaatg cactcggaa 
taatttact aatcatcag tagtctcga tcgtcaatg 
taggtagaa ttttttaat tttttaaga gcaaaagag 
aacagactt tttttatta aaaaattcc tagctttgc 
gttgaagct tttgtctaa aattttgta ctatataat 
tgatttttg tattttttt aattttaat tttaagtga 
cttgttata agctgatgt acaaatttt gttagtctt 
aataaatcc ttgtaaaaa taaaatcgt cactttcga 
atgattgtt ttgattcct accacgaac ggtttgagc 
cccttagct tatttttac aagagatta agtcataat 
acgcatagt tgaatagca atgtaggcc attaatttc 
cgaa
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