General information | |||
TC012532 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 11037415..11042919 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC012532 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_05261 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
larva appendages shortened - Penetrance (percentage of animals/eggs on slide): less than 30%
legs pretarsus partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
- >TC012532 RA
MSAVMDMYL SASDSMSFQ DILDIDIKS EIESLVGGH NDFSGLNFS ELPPLELED VSDIKWFSS NSNHSNSSL NLDFNGEER TTMVNPNAV MPAISLAQS IRSPSPTLK ESHLTFSPS TIKITTMKQ EANQVKKDL LKRLSDDDR EDKPPVLKT ISKVTPIIS KPPARTISK PTLLTFRNT INKQVNSPI TVAQVSHVT TVKTVTSTN RKLNNNRFS EDGERIYPK PAYSYSCLI AMALKNSRS GSLPVSEIY NFMCKHFPY FITAPNGWK NSVRHNLSL NKCFEKIEK PAINGAQRK GCLWAMNPA KISKMDEEV QKWSRKDPS AIRKAMINP ENLEPLERG ELKFSCSYD EGDTFGDSC GSAESEEGS DYEEGPKRD FEFVIKAEN EDGSDFDIE VNSDLDVEA SDEVYEDIE EDQQMLRVE MALAQKELM EYEKPVNSK RRRTYIVQN
- >TC012532 RA
atgtctgcc gtcatggac atgtacttg agcgcatcc gattcaatg tcatttcaa gacatcttg gacatcgac attaaatcc gaaatagaa tcgttggtc ggcggtcat aacgacttt tccggcttg aatttctcg gaattgcct ccactggaa ttggaagac gtttccgac ataaaatgg ttttcgtca aactcgaac cattcaaat tccagctta aatttagat tttaacggc gaagaacgg accacaatg gtcaatccg aacgccgta atgcctgct atttcctta gcccaaagt attcgttcg ccgtcaccg acgttaaaa gaaagtcac ttaacgttc tctccatcc actattaaa ataacaaca atgaaacaa gaagcgaat caagtgaag aaagatctt ttaaaacgg ctgagtgac gacgacagg gaagacaaa ccccctgta ttgaaaaca ataagtaaa gtcactcca atcatctca aaacctcca gctaggacc ataagcaaa ccgacgttg ttaactttt agaaataca attaataaa caagttaat tcccctatt acggtagct caggttagt cacgtcacc acagttaag acggtaact tccactaac cgaaaacta aacaataac cgattcagc gaagacggc gaacggatt tacccgaaa ccggcatac agttacagt tgtctcatc gcaatggcg ctgaaaaac agccgatcg ggtagttta cccgtctca gaaatctac aatttcatg tgtaaacac tttccgtac ttcataacg gcacctaac ggctggaaa aactcagtc agacacaat ctttcgctg aataaatgc ttcgaaaag atagagaaa ccggcgatc aacggggcg caaaggaaa ggctgtttg tgggcgatg aatcccgca aagatcagc aaaatggac gaggaagtc cagaaatgg tcccggaag gacccttca gccattagg aaggctatg atcaatcct gaaaatttg gaaccgctg gaaaggggc gagttgaag ttctcgtgc agttacgac gaaggggac acgtttggg gactcctgc gggagtgcc gaaagtgaa gaagggtcg gattatgag gaagggccg aaaagggac ttcgagttc gtgatcaag gcggagaac gaggacggc agcgacttc gatatcgaa gtcaatagt gatcttgat gttgaggct tccgacgag gtgtacgag gacatagag gaggatcag cagatgtta cgagtggag atggcgctc gctcagaag gagctaatg gagtacgag aagcccgtc aacagcaag agacggagg acgtacata gtccagaat taa
- >TC012532 RA
aggcgcatc aaaataatc tgcgaaaaa cgggcgaaa ttttggtgt gcgccattt cccgctttt ccgttcctc cagcgaaaa aacttaggt taaattaaa tgtcaagac cccattagc acgcgtatc gttattcga ttgcgaagt cggcgttcc ggcgactct cgccggcca atcagaatc gcgcgggaa acactcgtg acttgcgcc agccaatcg ggagcgcgc gatttgaat tccctcttc gttctttgt ggatgacga caagttttg tatacattc gttgggtgt aaagtgctg tcagtgttg aatatcttt ttatccatg atttaaaca aaaatacga ccagacaca acgaactgc cgtcaaata acgcatcaa agctaatcg tcatgtctg ccgtcatgg acatgtact tgagcgcat ccgattcaa tgtcatttc aagacatct tggacatcg acattaaat ccgaaatag aatcgttgg tcggcggtc ataacgact tttccggct tgaatttct cggaattgc ctccactgg aattggaag acgtttccg acataaaat ggttttcgt caaactcga accattcaa attccagct taaatttag attttaacg gcgaagaac ggaccacaa tggtcaatc cgaacgccg taatgcctg ctatttcct tagcccaaa gtattcgtt cgccgtcac cgacgttaa aagaaagtc acttaacgt tctctccat ccactatta aaataacaa caatgaaac aagaagcga atcaagtga agaaagatc ttttaaaac ggctgagtg acgacgaca gggaagaca aaccccctg tattgaaaa caataagta aagtcactc caatcatct caaaacctc cagctagga ccataagca aaccgacgt tgttaactt ttagaaata caattaata aacaagtta attccccta ttacggtag ctcaggtta gtcacgtca ccacagtta agacggtaa cttccacta accgaaaac taaacaata accgattca gcgaagacg gcgaacgga tttacccga aaccggcat acagttaca gttgtctca tcgcaatgg cgctgaaaa acagccgat cgggtagtt tacccgtct cagaaatct acaatttca tgtgtaaac actttccgt acttcataa cggcaccta acggctgga aaaactcag tcagacaca atctttcgc tgaataaat gcttcgaaa agatagaga aaccggcga tcaacgggg cgcaaagga aaggctgtt tgtgggcga tgaatcccg caaagatca gcaaaatgg acgaggaag tccagaaat ggtcccgga aggaccctt cagccatta ggaaggcta tgatcaatc ctgaaaatt tggaaccgc tggaaaggg gcgagttga agttctcgt gcagttacg acgaagggg acacgtttg gggactcct gcgggagtg ccgaaagtg aagaagggt cggattatg aggaagggc cgaaaaggg acttcgagt tcgtgatca aggcggaga acgaggacg gcagcgact tcgatatcg aagtcaata gtgatcttg atgttgagg cttccgacg aggtgtacg aggacatag aggaggatc agcagatgt tacgagtgg agatggcgc tcgctcaga aggagctaa tggagtacg agaagcccg tcaacagca agagacgga ggacgtaca tagtccaga attaagttg gtatttgta taaacagat cgtgtcggt gtccatctg gtcacacat ctccataag taaagtgaa gctgtgtaa gattggtga taattgtaa ataattgta actttcctg cccgtatat ttcttaaga ttagttgat tcattgttt tatacttga agactgccc tttcgattt tctttgtta aaatcaact agttttatg ttttatgaa acaatgaat tgagagaac agttttgtt gtattttgt aaaatttcg tcatttgcc aagacagta tttggaagt agatgtgga ctgatggca agagcaaaa ttgatcggt ccaaatgtg aatgtcgcc attcacatt cggaccgct tttgtgttt gtttacata acgtgcctt tgctccaaa ctcaatttt aggtctttg tatttcctc ttggtggga ccagttcac agaatttta tttgctctt attttattc gccacttaa tgtatcatg tgatagttt tctcatcca attcttgtt aatttattg tgtttccga ctgcgttaa gaaacgcgt ttcttaatg cactcggaa taatttact aatcatcag tagtctcga tcgtcaatg taggtagaa ttttttaat tttttaaga gcaaaagag aacagactt tttttatta aaaaattcc tagctttgc gttgaagct tttgtctaa aattttgta ctatataat tgatttttg tattttttt aattttaat tttaagtga cttgttata agctgatgt acaaatttt gttagtctt aataaatcc ttgtaaaaa taaaatcgt cactttcga atgattgtt ttgattcct accacgaac ggtttgagc cccttagct tatttttac aagagatta agtcataat acgcatagt tgaatagca atgtaggcc attaatttc cgaa
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
