Status
Please wait ...
Gene information
General information
TC034379
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC012530,TC012531
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 11030245..11036997
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034379
    iBeetle screen iB_05260

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    about 25

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult nutritional state - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034379 RA
      MSSSDQNNA LDMQAVGNF RQYLQIPSV HPNINYDSC 
      VKFLENQAK SLDLPIKTY FMVPKKPIV VLTWVGSEP 
      SLPSILLNS HMDVVPVFE DKWTHKPFS AHLDEQNNI 
      YARGTQDMK CVGIQYLEA VRRLKQQGV ALKRTLHIS 
      FAPDEEIGG GDGWKKFVH SKEFQELKV GATLDEGIT 
      CESDEFVVF YAERCPWQF HIHCPGKPG HGSLLLEDT 
      AGEKVSYIL SKLYEFRKG EKQKLDDNP TWTIGNVTA 
      LNLTQIKGG VQTNVVPPE FVITIDCRI PPNVDLEKF 
      EATLNQWCK EAGSGVWIE FIDKASHVT PTKLDDSNP 
      YWLAFKKAT DKLGLKLKP QISPAVSDT SHIRRVGLP 
      GIGFSPINH TSVALHKHD EYLPVQVFL KGIEIYCEI 
      LKSLGNVEE TTKEKTNFS RKMSSLSAV QKDALDTQA 
      VNNFREYLR IPSVHPDVN YDACVTFLE NQAKGLGLP 
      VKIYHVVPK KPIVVLTWV GTQPHLPSV LLNSHMDVV 
      PVFEDKWTH KPFSADIDE QNNIYARGT QDMKCVGIQ 
      YLEAVRRLK QQGVTLKRT LHISFVPDE EIGGEDGMR 
      KFVHTKEFK DLNVGFALD EGMASPNEE FAVFNGERC 
      IWHLHIHCP GQPGHGSLL LDNTAGEKV TYLLNKFFE 
      FRRQEKKKL KDNPSRTIG DVTTVNLTQ MTGGVQTNV 
      VPPEFVIAI DCRIPPETV DIKKWEETI NQWCKEAGP 
      GIWIEYEQK QAQIPPTKV DSSNPFWVA FKEGTDKLG 
      LKLKTQIFP GGTDSRYVR GIGIPAIGF SPINNTPVL 
      LHDHDEYLG VNTFLKGIE IYCKILISV ANVEDRSG
    CDS sequence
    • >TC034379 RA
      atgtcatca agcgaccaa aacaatgcc ttggatatg 
      caagctgtt ggaaatttc cgccaatac ttgcaaatc 
      ccttcggtg cacccaaac ataaattat gattcatgc 
      gtcaagttt ctggaaaac caggccaag agcctggac 
      ctccccata aaaacctac ttcatggtg cccaaaaaa 
      cccatcgtg gtgctcaca tgggtgggc agcgaaccc 
      agcttaccg tcgattttg ctcaacagc cacatggac 
      gtggtgccg gttttcgag gataaatgg acccacaaa 
      cccttcagt gcccatctg gacgagcaa aacaacatc 
      tacgccaga ggcacccaa gacatgaaa tgtgtggga 
      atccaatac ttggaagct gttcgaaga ctaaaacag 
      caaggggtg gctttgaag cggacgctc cacatttcg 
      ttcgctcct gatgaggaa atcgggggt ggagatggg 
      tggaaaaaa ttcgtccac agtaaagag tttcaagag 
      ttgaaagtt ggggcgacg ctagatgag ggaataacg 
      tgcgaaagc gacgagttt gttgttttc tatgctgag 
      cgttgccct tggcagttt cacatccat tgccccggg 
      aaacctgga catgggtcc ctccttctg gaggacaca 
      gcaggggaa aaagtttcc tatattttg tccaaactg 
      tacgagttc agaaaaggg gagaagcaa aaactggac 
      gataacccg acttggacc attgggaac gtcacagca 
      ttgaattta acccaaatc aaaggtggg gtacaaact 
      aacgtagtg ccccctgaa ttcgtcata acgatcgat 
      tgtcgaatc cctccaaac gttgacttg gaaaaattc 
      gaagcaact ctgaaccaa tggtgcaaa gaagcaggt 
      tcaggcgtt tggatcgaa ttcatagat aaagcctca 
      catgtcaca ccaactaaa ctagacgat tcaaatccc 
      tattggctc gcatttaaa aaagcaact gataaactg 
      ggtttaaaa ctgaaacca caaatttct ccagctgtt 
      agtgatact agtcatata cgaagagtt ggactgcca 
      ggaataggg ttctcacca atcaatcat acctcagtg 
      gctttacat aaacatgat gagtatcta cctgttcaa 
      gtttttttg aaaggcatt gaaatctac tgcgaaatt 
      ttgaaatct ctcggaaac gttgaagaa acaacgaaa 
      gaaaaaaca aattttagt cgtaagatg tcttcattg 
      tcagctgtt cagaaagac gcgttggac acacaagct 
      gtcaacaat tttcgtgaa tatttgcgg attccttcc 
      gtacatccg gacgttaac tacgatgcc tgcgtcaca 
      ttcctcgaa aaccaggcc aaaggcttg ggccttccc 
      gtcaaaatc taccacgta gtgcccaag aaacccatc 
      gtggtgctg acatgggtt ggaacccag ccccactta 
      ccatcagtc ctcctcaac agccacatg gacgtggtg 
      cccgttttc gaagacaag tggacccat aagcccttc 
      agtgccgac atagacgag caaaataac atctatgca 
      aggggcacc caagacatg aaatgtgtg ggaatccaa 
      tatttggaa gctgtgaga agattaaaa cagcaaggg 
      gtgactctc aagcgaaca ttgcatatt tctttcgtc 
      cctgatgag gaaattggg ggtgaagat gggatgaga 
      aagttcgtt catacaaag gagttcaag gatttgaac 
      gttgggttt gctttggac gaagggatg gcgagtccg 
      aatgaagag tttgcggtt tttaatggg gagaggtgt 
      atttggcat ttacacatc cactgcccc ggccagcca 
      ggccatggc tccttgctc ctggacaac acagcggga 
      gaaaaagtc acctatctt ttaaacaaa tttttcgaa 
      ttcagaaga caggaaaaa aagaaactc aaagacaat 
      cctagccgg accatcggt gacgtcacg acagtcaat 
      ttgacccaa atgacaggc ggcgtacaa actaacgta 
      gtgccccct gaattcgtc atcgccatc gactgccgc 
      attcccccc gaaaccgtc gacataaaa aaatgggag 
      gagaccatc aaccagtgg tgcaaggaa gccgggcct 
      ggaatctgg atcgagtac gagcaaaaa caggcccaa 
      attccgccc actaaggtc gattcctct aatcccttc 
      tgggtggct tttaaggag ggaacagac aaattgggc 
      ctaaaactc aaaacccag atattccca gggggcacc 
      gacagccgc tacgtgcga ggtatcggc ataccagcg 
      attggcttt tcgcctatt aataacacc ccggttttg 
      ttacacgac catgatgag tatttggga gtcaacacg 
      tttttaaaa ggtatagaa atctattgc aaaatcctg 
      atttctgtt gcaaatgtg gaggaccga tcagggtaa
    mRNA sequence
    • >TC034379 RA
      acgaaccaa aatatgtca tcaagcgac caaaacaat 
      gccttggat atgcaagct gttggaaat ttccgccaa 
      tacttgcaa atcccttcg gtgcaccca aacataaat 
      tatgattca tgcgtcaag tttctggaa aaccaggcc 
      aagagcctg gacctcccc ataaaaacc tacttcatg 
      gtgcccaaa aaacccatc gtggtgctc acatgggtg 
      ggcagcgaa cccagctta ccgtcgatt ttgctcaac 
      agccacatg gacgtggtg ccggttttc gaggataaa 
      tggacccac aaacccttc agtgcccat ctggacgag 
      caaaacaac atctacgcc agaggcacc caagacatg 
      aaatgtgtg ggaatccaa tacttggaa gctgttcga 
      agactaaaa cagcaaggg gtggctttg aagcggacg 
      ctccacatt tcgttcgct cctgatgag gaaatcggg 
      ggtggagat gggtggaaa aaattcgtc cacagtaaa 
      gagtttcaa gagttgaaa gttggggcg acgctagat 
      gagggaata acgtgcgaa agcgacgag tttgttgtt 
      ttctatgct gagcgttgc ccttggcag tttcacatc 
      cattgcccc gggaaacct ggacatggg tccctcctt 
      ctggaggac acagcaggg gaaaaagtt tcctatatt 
      ttgtccaaa ctgtacgag ttcagaaaa ggggagaag 
      caaaaactg gacgataac ccgacttgg accattggg 
      aacgtcaca gcattgaat ttaacccaa atcaaaggt 
      ggggtacaa actaacgta gtgccccct gaattcgtc 
      ataacgatc gattgtcga atccctcca aacgttgac 
      ttggaaaaa ttcgaagca actctgaac caatggtgc 
      aaagaagca ggttcaggc gtttggatc gaattcata 
      gataaagcc tcacatgtc acaccaact aaactagac 
      gattcaaat ccctattgg ctcgcattt aaaaaagca 
      actgataaa ctgggttta aaactgaaa ccacaaatt 
      tctccagct gttagtgat actagtcat atacgaaga 
      gttggactg ccaggaata gggttctca ccaatcaat 
      catacctca gtggcttta cataaacat gatgagtat 
      ctacctgtt caagttttt ttgaaaggc attgaaatc 
      tactgcgaa attttgaaa tctctcgga aacgttgaa 
      gaaacaacg aaagaaaaa acaaatttt agtcgtaag 
      atgtcttca ttgtcagct gttcagaaa gacgcgttg 
      gacacacaa gctgtcaac aattttcgt gaatatttg 
      cggattcct tccgtacat ccggacgtt aactacgat 
      gcctgcgtc acattcctc gaaaaccag gccaaaggc 
      ttgggcctt cccgtcaaa atctaccac gtagtgccc 
      aagaaaccc atcgtggtg ctgacatgg gttggaacc 
      cagccccac ttaccatca gtcctcctc aacagccac 
      atggacgtg gtgcccgtt ttcgaagac aagtggacc 
      cataagccc ttcagtgcc gacatagac gagcaaaat 
      aacatctat gcaaggggc acccaagac atgaaatgt 
      gtgggaatc caatatttg gaagctgtg agaagatta 
      aaacagcaa ggggtgact ctcaagcga acattgcat 
      atttctttc gtccctgat gaggaaatt gggggtgaa 
      gatgggatg agaaagttc gttcataca aaggagttc 
      aaggatttg aacgttggg tttgctttg gacgaaggg 
      atggcgagt ccgaatgaa gagtttgcg gtttttaat 
      ggggagagg tgtatttgg catttacac atccactgc 
      cccggccag ccaggccat ggctccttg ctcctggac 
      aacacagcg ggagaaaaa gtcacctat cttttaaac 
      aaatttttc gaattcaga agacaggaa aaaaagaaa 
      ctcaaagac aatcctagc cggaccatc ggtgacgtc 
      acgacagtc aatttgacc caaatgaca ggcggcgta 
      caaactaac gtagtgccc cctgaattc gtcatcgcc 
      atcgactgc cgcattccc cccgaaacc gtcgacata 
      aaaaaatgg gaggagacc atcaaccag tggtgcaag 
      gaagccggg cctggaatc tggatcgag tacgagcaa 
      aaacaggcc caaattccg cccactaag gtcgattcc 
      tctaatccc ttctgggtg gcttttaag gagggaaca 
      gacaaattg ggcctaaaa ctcaaaacc cagatattc 
      ccagggggc accgacagc cgctacgtg cgaggtatc 
      ggcatacca gcgattggc ttttcgcct attaataac 
      accccggtt ttgttacac gaccatgat gagtatttg 
      ggagtcaac acgttttta aaaggtata gaaatctat 
      tgcaaaatc ctgatttct gttgcaaat gtggaggac 
      cgatcaggg taaagggct ttttgatgt tattgagtt 
      gagagttta ataaaatat cttctggtg caataaaaa 
      catggttta ttc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY