| General information | |||
| TC012518 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 10903855..10948727 | |
| Sequence information | |||
| Transcripts / Proteins (3) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC012518 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05257 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)Days post injection
adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
Days post injection
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 20
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
- >TC012518 RA
MEPDHRDHR CHRPSAFDK MEGLVREMQ DTEHGGVPV RSQKLFLTS IPAAFMGYD LIEWLMERL GIEESEALN LANQLCQYG YFFPVSDSK NLVVKDDSS LYRFQSPYY WPWQNRPPD NIEYAIYLA KRTLRNKQR HGLEDYELE ALSNLRKNL ANKWDFITM QAEEQVKLS KVLKKGDKI ISDSQERAY WRVHRPPPG MVTSLEQCP VPTRSWNGS RTRKRTIED GRREVELLK NSLSRTRVK VSQALESMV QHVETYAEY DPLITPTQP SNPWVSEDT TYWQLNSPL VEVPTEKRV RRWGLSMEE LVSDPTGLQ EFTNYLRKE YSHENIRFW MAVNDLRRS AQSQIQRKV NEIFEEFLA PGAPCEINI DGKTMEKVH QEMKTPSRF TFDAAQEHV YTLLLKKDC YPRFIRSEY YKNLLATGI QPSQKKRFF GFGQTKKKT STSSAPNQQ LLQQQPSQG ASVTCGALS KRRGSDRSL SGSAHELAV SGVKDSKVP HSHSQSNLS DIPYRGDLA CLPKASPVP TSVTSPVKK PSIAIVAGA STSEEVCPW ESGEPRSRK NSAQLDSGS SSSDISVAV AEVSERVQR VCTLTQQHT VDGSRKLST STMDPPRRA SVSVPITHS TTGDSSKRK VSSFEDNSS AATSTSSSV FVIPSDTDN KSEECTSTN KTVVKNHGL AKACSVTGA NAPIISVSS VADDLPPDS KEEQVEPTT SQEPLAPLA EPDSESPAS ELPPSEPIT EVEADTEAK SNDVCPWED EETCKVDTP FVKTYATLG YL
- >TC012518 RB
MEPDHRDHR CHRPSAFDK MEGLVREMQ DTEHGGVPV RSQKLFLTS IPAAFMGYD LIEWLMERL GIEESEALN LANQLCQYG YFFPVSDSK NLVVKDDSS LYRFQSPYY WPWQNRPPD NIEYAIYLA KRTLRNKQR HGLEDYELE ALSNLRKNL ANKWDFITM QAEEQVKLS KVLKKGDKI ISDSQERAY WRVHRPPPG MVTSLEQCP VPTRSWNGS RTRKRTIED GRREVELLK NSLSRTRVK VSQALESMV QHVETYAEY DPLITPTQP SNPWVSEDT TYWQLNSPL VEVPTEKRV RRWGLSMEE LVSDPTGLQ EFTNYLRKE YSHENIRFW MAVNDLRRS AQSQIQRKV NEIFEEFLA PGAPCEINI DGKTMEKVH QEMKTPSRF TFDAAQEHV YTLLLKKDC YPRFIRSEY YKNLLATGI QPSQKKRFF GFGQTKKKT STSSAPNQQ LLQQQPSQG ASVTCGALS KRRGSDRSL SGSAHELAV SGVKDSKVP HSHSQSNLS DIPYRGDLA CLPKASPVP TSVTSPVKK PSIAIVAGA STSEEVCPW ESGEPRSRK NSAQLDSGS SSSDISVAV AEVSERVQR VCTLTQQHT VDGSRKLST STMDPPRRA SVSVPITHS TTGDSSKRK VSSFEDNSS AATSTSSSV FVIPSDTDN KSEECTSTN KTVVKNHGL AKACSVTGA NAPIISVSS VADDLPPDS KEEQVEPTT SQEPLAPLA EPDSESPAS ELPPSEPIT EVEADTEAK SNDVCPWED E
- >TC012518 RC
MEPDHRDHR CHRPSAFDK MEGLVREMQ DTEHGGVPV RSQKLFLTS IPAAFMGYD LIEWLMERL GIEESEALN LANQLCQYG YFFPVSDSK NLVVKDDSS LYRFQSPYY WPWQNRPPD NIEYAIYLA KRTLRNKQR HGLEDYELE ALSNLRKNL ANKWDFITM QAEEQVKLS KVLKKGDKI ISDSQERAY WRVHRPPPG MVTSLEQCP VPTRSWNGS RTRKRTIED GRREVELLK NSLSRTRVK VSQALESMV QHVETYAEY DPLITPTQP SNPWVSEDT TYWQLNSPL VEVPTEKRV RRWGLSMEE LVSDPTGLQ EFTNYLRKE YSHENIRFW MAVNDLRRS AQSQIQRKV NEIFEEFLA PGAPCEINI DGKTMEKVH QEMKTPSRF TFDAAQEHV YTLLLKKDC YPRFIRSEY YKNLLATGI QPSQKKRFF GFGQTKKKT STSSAPNQQ LLQQQPSQG ASVTCGALS KRRGSDRSL SGSAHELAV SGVKDSKVP HSHSQSNLS DIPYRVKKP SIAIVAGAS TSEEVCPWE SGEPRSRKN SAQLDSGSS SSDISVAVA EVSERVQRV CTLTQQHTV DGSRKLSTS TMDPPRRAS VSVPITHST TGDSSKRKV SSFEDNSSA ATSTSSSVF VIPSDTDNK SEECTSTNK TVVKNHGLA KACSVTGAN APIISVSSV ADDLPPDSK EEQVEPTTS QEPLAPLAE PDSESPASE LPPSEPITE VEADTEAKS NDVCPWEDE ETCKVDTPF VKTYATLGY L
- >TC012518 RA
atggagccg gaccaccgg gaccaccga tgccacagg cccagcgcc tttgacaaa atggagggg ttggtgaga gagatgcag gacacggag cacgggggc gttccagtt cgaagccaa aaattgttc cttacatcc attcccgcc gctttcatg gggtacgac ctaattgaa tggctgatg gagcgactc gggatcgaa gaatccgag gccctcaat ctagccaat cagctgtgt cagtatggc tactttttt cccgtgagc gattcgaaa aacctggtt gtgaaagat gatagctcg ctgtatagg tttcaatct ccgtattat tggccttgg cagaacaga ccgccggat aacatcgaa tacgcgatt tacttagcg aaaagaact ttacggaat aagcagagg cacggtctg gaggattac gagttggaa gctttgagc aacttgcgg aaaaacttg gcgaacaag tgggatttt attacaatg caggctgag gagcaggtg aagctgtcg aaggtgctg aagaagggc gacaagatc atcagcgac tcgcaggag agggcgtac tggcgcgtc cacaggcct ccgccgggg atggtcacc tcgctggag cagtgcccg gtgccgacg cgcagctgg aacggctcc aggacccgg aaacgcacc atcgaggat ggcaggcga gaagtcgag ttgttgaag aacagtctg tcaaggact agggttaag gtgtcgcaa gcgctggag agtatggtc cagcatgtc gagacctac gctgagtac gacccttta ataacacca acgcaacct tccaaccct tgggtcagc gaggacacc acgtactgg caattgaac agtccacta gtcgaagtt ccgactgag aaacgcgtg aggcggtgg ggcctatcc atggaggag ctagtttca gatccgaca ggtttacaa gagtttact aattatttg aggaaggag tacagccac gaaaacatc aggttctgg atggccgtg aatgattta agacgttct gctcaatcc caaatccag agaaaagtt aacgaaatt tttgaggaa tttttggcc cctggtgct ccgtgtgag ataaatatc gacggtaaa accatggag aaagtccac caagagatg aaaacaccc agcaggttc accttcgac gctgcacaa gaacatgta tatacattg ttgctgaaa aaagattgt tatcctaga tttattagg tccgaatat tataagaat ctactggcg acgggaatc cagccttcc cagaaaaag cgctttttc ggtttcggc caaaccaag aagaaaacc tcgacgagt agtgccccc aaccagcaa ctgctgcag cagcagccc tcccagggg gcctcggtg acttgcgga gccctgtcg aagcgcagg ggcagcgac cgcagtctc tcaggatcg gcccacgag ctcgccgtt tccggggtg aaagactcc aaggtgcca cactcacac tcgcagagt aacttgagc gacatcccc tacaggggc gatttggcg tgccttccg aaagccagt ccggtgcca acatcggtt accagtcca gtgaagaag ccaagcatt gcgatcgtg gccggcgcc tccacctcg gaggaggtg tgtccgtgg gagagcggc gagccccgt tcccggaaa aactcggcg cagctcgac tccggcagc tcctcgtcg gacatcagc gtggcggtg gcggaggtc tccgagcgg gtccagcgc gtttgcact ctgactcag caacacacc gtcgacggc agcagaaaa ctctcgacc tccaccatg gaccctccc cggagagca tccgtttcc gtgccaatc actcactca accaccgga gactcctcc aagcggaag gtgtcctcc ttcgaggac aacagctcc gccgcgacc agcacgtcg tcatcggtg ttcgttatt cctagtgac acagacaat aaatctgaa gaatgcact agtactaat aaaactgtt gttaagaac cacgggctt gcaaaggct tgttcagtg acaggggcg aatgcacct attatcagt gtcagttcg gtggccgac gatttgccc cccgatagc aaggaggag caagtcgaa cccaccacc agtcaggag ccgctagcg ccgctcgct gagcccgac agtgaatcg ccagccagc gaactgcct ccttctgaa cccatcact gaagtcgag gccgacacg gaggccaag agtaacgac gtctgtccc tgggaagac gaggaaacg tgtaaggtg gacacgccc ttcgtcaag acttacgct actctgggc tacctttag
- >TC012518 RB
atggagccg gaccaccgg gaccaccga tgccacagg cccagcgcc tttgacaaa atggagggg ttggtgaga gagatgcag gacacggag cacgggggc gttccagtt cgaagccaa aaattgttc cttacatcc attcccgcc gctttcatg gggtacgac ctaattgaa tggctgatg gagcgactc gggatcgaa gaatccgag gccctcaat ctagccaat cagctgtgt cagtatggc tactttttt cccgtgagc gattcgaaa aacctggtt gtgaaagat gatagctcg ctgtatagg tttcaatct ccgtattat tggccttgg cagaacaga ccgccggat aacatcgaa tacgcgatt tacttagcg aaaagaact ttacggaat aagcagagg cacggtctg gaggattac gagttggaa gctttgagc aacttgcgg aaaaacttg gcgaacaag tgggatttt attacaatg caggctgag gagcaggtg aagctgtcg aaggtgctg aagaagggc gacaagatc atcagcgac tcgcaggag agggcgtac tggcgcgtc cacaggcct ccgccgggg atggtcacc tcgctggag cagtgcccg gtgccgacg cgcagctgg aacggctcc aggacccgg aaacgcacc atcgaggat ggcaggcga gaagtcgag ttgttgaag aacagtctg tcaaggact agggttaag gtgtcgcaa gcgctggag agtatggtc cagcatgtc gagacctac gctgagtac gacccttta ataacacca acgcaacct tccaaccct tgggtcagc gaggacacc acgtactgg caattgaac agtccacta gtcgaagtt ccgactgag aaacgcgtg aggcggtgg ggcctatcc atggaggag ctagtttca gatccgaca ggtttacaa gagtttact aattatttg aggaaggag tacagccac gaaaacatc aggttctgg atggccgtg aatgattta agacgttct gctcaatcc caaatccag agaaaagtt aacgaaatt tttgaggaa tttttggcc cctggtgct ccgtgtgag ataaatatc gacggtaaa accatggag aaagtccac caagagatg aaaacaccc agcaggttc accttcgac gctgcacaa gaacatgta tatacattg ttgctgaaa aaagattgt tatcctaga tttattagg tccgaatat tataagaat ctactggcg acgggaatc cagccttcc cagaaaaag cgctttttc ggtttcggc caaaccaag aagaaaacc tcgacgagt agtgccccc aaccagcaa ctgctgcag cagcagccc tcccagggg gcctcggtg acttgcgga gccctgtcg aagcgcagg ggcagcgac cgcagtctc tcaggatcg gcccacgag ctcgccgtt tccggggtg aaagactcc aaggtgcca cactcacac tcgcagagt aacttgagc gacatcccc tacaggggc gatttggcg tgccttccg aaagccagt ccggtgcca acatcggtt accagtcca gtgaagaag ccaagcatt gcgatcgtg gccggcgcc tccacctcg gaggaggtg tgtccgtgg gagagcggc gagccccgt tcccggaaa aactcggcg cagctcgac tccggcagc tcctcgtcg gacatcagc gtggcggtg gcggaggtc tccgagcgg gtccagcgc gtttgcact ctgactcag caacacacc gtcgacggc agcagaaaa ctctcgacc tccaccatg gaccctccc cggagagca tccgtttcc gtgccaatc actcactca accaccgga gactcctcc aagcggaag gtgtcctcc ttcgaggac aacagctcc gccgcgacc agcacgtcg tcatcggtg ttcgttatt cctagtgac acagacaat aaatctgaa gaatgcact agtactaat aaaactgtt gttaagaac cacgggctt gcaaaggct tgttcagtg acaggggcg aatgcacct attatcagt gtcagttcg gtggccgac gatttgccc cccgatagc aaggaggag caagtcgaa cccaccacc agtcaggag ccgctagcg ccgctcgct gagcccgac agtgaatcg ccagccagc gaactgcct ccttctgaa cccatcact gaagtcgag gccgacacg gaggccaag agtaacgac gtctgtccc tgggaagac gagtaa
- >TC012518 RC
atggagccg gaccaccgg gaccaccga tgccacagg cccagcgcc tttgacaaa atggagggg ttggtgaga gagatgcag gacacggag cacgggggc gttccagtt cgaagccaa aaattgttc cttacatcc attcccgcc gctttcatg gggtacgac ctaattgaa tggctgatg gagcgactc gggatcgaa gaatccgag gccctcaat ctagccaat cagctgtgt cagtatggc tactttttt cccgtgagc gattcgaaa aacctggtt gtgaaagat gatagctcg ctgtatagg tttcaatct ccgtattat tggccttgg cagaacaga ccgccggat aacatcgaa tacgcgatt tacttagcg aaaagaact ttacggaat aagcagagg cacggtctg gaggattac gagttggaa gctttgagc aacttgcgg aaaaacttg gcgaacaag tgggatttt attacaatg caggctgag gagcaggtg aagctgtcg aaggtgctg aagaagggc gacaagatc atcagcgac tcgcaggag agggcgtac tggcgcgtc cacaggcct ccgccgggg atggtcacc tcgctggag cagtgcccg gtgccgacg cgcagctgg aacggctcc aggacccgg aaacgcacc atcgaggat ggcaggcga gaagtcgag ttgttgaag aacagtctg tcaaggact agggttaag gtgtcgcaa gcgctggag agtatggtc cagcatgtc gagacctac gctgagtac gacccttta ataacacca acgcaacct tccaaccct tgggtcagc gaggacacc acgtactgg caattgaac agtccacta gtcgaagtt ccgactgag aaacgcgtg aggcggtgg ggcctatcc atggaggag ctagtttca gatccgaca ggtttacaa gagtttact aattatttg aggaaggag tacagccac gaaaacatc aggttctgg atggccgtg aatgattta agacgttct gctcaatcc caaatccag agaaaagtt aacgaaatt tttgaggaa tttttggcc cctggtgct ccgtgtgag ataaatatc gacggtaaa accatggag aaagtccac caagagatg aaaacaccc agcaggttc accttcgac gctgcacaa gaacatgta tatacattg ttgctgaaa aaagattgt tatcctaga tttattagg tccgaatat tataagaat ctactggcg acgggaatc cagccttcc cagaaaaag cgctttttc ggtttcggc caaaccaag aagaaaacc tcgacgagt agtgccccc aaccagcaa ctgctgcag cagcagccc tcccagggg gcctcggtg acttgcgga gccctgtcg aagcgcagg ggcagcgac cgcagtctc tcaggatcg gcccacgag ctcgccgtt tccggggtg aaagactcc aaggtgcca cactcacac tcgcagagt aacttgagc gacatcccc tacagagtg aagaagcca agcattgcg atcgtggcc ggcgcctcc acctcggag gaggtgtgt ccgtgggag agcggcgag ccccgttcc cggaaaaac tcggcgcag ctcgactcc ggcagctcc tcgtcggac atcagcgtg gcggtggcg gaggtctcc gagcgggtc cagcgcgtt tgcactctg actcagcaa cacaccgtc gacggcagc agaaaactc tcgacctcc accatggac cctccccgg agagcatcc gtttccgtg ccaatcact cactcaacc accggagac tcctccaag cggaaggtg tcctccttc gaggacaac agctccgcc gcgaccagc acgtcgtca tcggtgttc gttattcct agtgacaca gacaataaa tctgaagaa tgcactagt actaataaa actgttgtt aagaaccac gggcttgca aaggcttgt tcagtgaca ggggcgaat gcacctatt atcagtgtc agttcggtg gccgacgat ttgcccccc gatagcaag gaggagcaa gtcgaaccc accaccagt caggagccg ctagcgccg ctcgctgag cccgacagt gaatcgcca gccagcgaa ctgcctcct tctgaaccc atcactgaa gtcgaggcc gacacggag gccaagagt aacgacgtc tgtccctgg gaagacgag gaaacgtgt aaggtggac acgcccttc gtcaagact tacgctact ctgggctac ctttag
- >TC012518 RA
ttccgaatg caattttca ttacaatac gtgtgtatt ttcgtgtgc catttcgcg atcaataat ttcatgtcg agtcctggg aaagtcggg cgcgcgcac ctcttctct tcgccggat cgccattac agtcccaga tttcgctga gcgaagttg tctcgcgcg gcggaaacg cggctcggt acgcaacct gcatgcggg attaccact tccggtccc gttaacgca tccggttcg agccgttgg gacacgagg cggtgctaa ccgaccctc tcggtgtat ggtgtaaga tacagtggc ctcggatga agtccaacg cgcgatccg ctgttgttc gttgtatgt gggggtgag tgtcggcgt gatggggcg ggggcagag ctcgcccat ggagctcgg ctagaggcg tgcgcccct gcccggctg cccggcccc ccctctgcc gccctcacc acggcccac gcatggagc cggaccacc gggaccacc gatgccaca ggcccagcg cctttgaca aaatggagg ggttggtga gagagatgc aggacacgg agcacgggg gcgttccag ttcgaagcc aaaaattgt tccttacat ccattcccg ccgctttca tggggtacg acctaattg aatggctga tggagcgac tcgggatcg aagaatccg aggccctca atctagcca atcagctgt gtcagtatg gctactttt ttcccgtga gcgattcga aaaacctgg ttgtgaaag atgatagct cgctgtata ggtttcaat ctccgtatt attggcctt ggcagaaca gaccgccgg ataacatcg aatacgcga tttacttag cgaaaagaa ctttacgga ataagcaga ggcacggtc tggaggatt acgagttgg aagctttga gcaacttgc ggaaaaact tggcgaaca agtgggatt ttattacaa tgcaggctg aggagcagg tgaagctgt cgaaggtgc tgaagaagg gcgacaaga tcatcagcg actcgcagg agagggcgt actggcgcg tccacaggc ctccgccgg ggatggtca cctcgctgg agcagtgcc cggtgccga cgcgcagct ggaacggct ccaggaccc ggaaacgca ccatcgagg atggcaggc gagaagtcg agttgttga agaacagtc tgtcaagga ctagggtta aggtgtcgc aagcgctgg agagtatgg tccagcatg tcgagacct acgctgagt acgaccctt taataacac caacgcaac cttccaacc cttgggtca gcgaggaca ccacgtact ggcaattga acagtccac tagtcgaag ttccgactg agaaacgcg tgaggcggt ggggcctat ccatggagg agctagttt cagatccga caggtttac aagagttta ctaattatt tgaggaagg agtacagcc acgaaaaca tcaggttct ggatggccg tgaatgatt taagacgtt ctgctcaat cccaaatcc agagaaaag ttaacgaaa tttttgagg aatttttgg cccctggtg ctccgtgtg agataaata tcgacggta aaaccatgg agaaagtcc accaagaga tgaaaacac ccagcaggt tcaccttcg acgctgcac aagaacatg tatatacat tgttgctga aaaaagatt gttatccta gatttatta ggtccgaat attataaga atctactgg cgacgggaa tccagcctt cccagaaaa agcgctttt tcggtttcg gccaaacca agaagaaaa cctcgacga gtagtgccc ccaaccagc aactgctgc agcagcagc cctcccagg gggcctcgg tgacttgcg gagccctgt cgaagcgca ggggcagcg accgcagtc tctcaggat cggcccacg agctcgccg tttccgggg tgaaagact ccaaggtgc cacactcac actcgcaga gtaacttga gcgacatcc cctacaggg gcgatttgg cgtgccttc cgaaagcca gtccggtgc caacatcgg ttaccagtc cagtgaaga agccaagca ttgcgatcg tggccggcg cctccacct cggaggagg tgtgtccgt gggagagcg gcgagcccc gttcccgga aaaactcgg cgcagctcg actccggca gctcctcgt cggacatca gcgtggcgg tggcggagg tctccgagc gggtccagc gcgtttgca ctctgactc agcaacaca ccgtcgacg gcagcagaa aactctcga cctccacca tggaccctc cccggagag catccgttt ccgtgccaa tcactcact caaccaccg gagactcct ccaagcgga aggtgtcct ccttcgagg acaacagct ccgccgcga ccagcacgt cgtcatcgg tgttcgtta ttcctagtg acacagaca ataaatctg aagaatgca ctagtacta ataaaactg ttgttaaga accacgggc ttgcaaagg cttgttcag tgacagggg cgaatgcac ctattatca gtgtcagtt cggtggccg acgatttgc cccccgata gcaaggagg agcaagtcg aacccacca ccagtcagg agccgctag cgccgctcg ctgagcccg acagtgaat cgccagcca gcgaactgc ctccttctg aacccatca ctgaagtcg aggccgaca cggaggcca agagtaacg acgtctgtc cctgggaag acgaggaaa cgtgtaagg tggacacgc ccttcgtca agacttacg ctactctgg gctaccttt agctaccgc aactgtcaa gagatcaag tgacgtttg ctcacaact tctatttga ttttcttaa ttttgtttc aagtttacc tacccaaag ttaggtatt gtcaaaaac aagtttgat tatgtcagg tttagcggg gaaaaatga ttccaaagg acggtttta agagtggag gttcaccag agaagggtc aaattttca aacggttta gggcataac ggaacggta acaatgtcg gcattagaa atagacttt cttgagccc ttggtcatt atctgatgt tgacagctt cacgttccg ccgtcccct actaaagtc aggaactgc acctgattc ttctgtacc agtacactc tcaatataa atacgtggc attgtcgta ttataatca atgtaactg taggtgttg aaaaaaaat aaaaacgga tagggttga ttaacggtg cagttatcg actttccaa tatccacat aaaaattga tgtttgaac actttcgta agtaatcga gcacaatat tctcttaat gtcgtatcg ttgtattag atatagagc atttaatta tacgtattg tagcctagt ggtgtatca atgttcagt gcagtttct aactttccc aaaactttc ggccaagtt tccaaaatt caaaatttt tcgttcggg ttcgcgatt tacaaaagg gaaaatctc ctttggaat catctcctt ccgacgatg tttcagttc cagtccccg gaataaatt gcgattgtg agcaacaca tttccaagt taacttaaa tgtatccag gccattttg atgcaaatt ctggcctat atacgtatt tgttgttta ataaacgta ggttgactg ttattgctt gacacaaaa atgttaaaa aaaatctga tggcctcat taatatttg atagaagta ccagtggtg tatataaaa ccctccgcc tccagctgg ggtttcaat taaatactc aattaaagt taacattat attcaaaca tgcaatgtt acttattga agctatttt tatgataaa tgttttcgt ctcttagaa tattgtggt aagcgtcaa aatatatat gctgcctga cagtgagag agttagtga tgtatatag ttttataat aatagctat tataacgta ttattaaaa atgttacca cttaatgtc ctaaaacat tttatttca tagaattgt tgataggca atgttatta taaaattat tgtagatta atgaagtaa aagaaatgt gatttaatc tc
- >TC012518 RB
ttccgaatg caattttca ttacaatac gtgtgtatt ttcgtgtgc catttcgcg atcaataat ttcatgtcg agtcctggg aaagtcggg cgcgcgcac ctcttctct tcgccggat cgccattac agtcccaga tttcgctga gcgaagttg tctcgcgcg gcggaaacg cggctcggt acgcaacct gcatgcggg attaccact tccggtccc gttaacgca tccggttcg agccgttgg gacacgagg cggtgctaa ccgaccctc tcggtgtat ggtgtaaga tacagtggc ctcggatga agtccaacg cgcgatccg ctgttgttc gttgtatgt gggggtgag tgtcggcgt gatggggcg ggggcagag ctcgcccat ggagctcgg ctagaggcg tgcgcccct gcccggctg cccggcccc ccctctgcc gccctcacc acggcccac gcatggagc cggaccacc gggaccacc gatgccaca ggcccagcg cctttgaca aaatggagg ggttggtga gagagatgc aggacacgg agcacgggg gcgttccag ttcgaagcc aaaaattgt tccttacat ccattcccg ccgctttca tggggtacg acctaattg aatggctga tggagcgac tcgggatcg aagaatccg aggccctca atctagcca atcagctgt gtcagtatg gctactttt ttcccgtga gcgattcga aaaacctgg ttgtgaaag atgatagct cgctgtata ggtttcaat ctccgtatt attggcctt ggcagaaca gaccgccgg ataacatcg aatacgcga tttacttag cgaaaagaa ctttacgga ataagcaga ggcacggtc tggaggatt acgagttgg aagctttga gcaacttgc ggaaaaact tggcgaaca agtgggatt ttattacaa tgcaggctg aggagcagg tgaagctgt cgaaggtgc tgaagaagg gcgacaaga tcatcagcg actcgcagg agagggcgt actggcgcg tccacaggc ctccgccgg ggatggtca cctcgctgg agcagtgcc cggtgccga cgcgcagct ggaacggct ccaggaccc ggaaacgca ccatcgagg atggcaggc gagaagtcg agttgttga agaacagtc tgtcaagga ctagggtta aggtgtcgc aagcgctgg agagtatgg tccagcatg tcgagacct acgctgagt acgaccctt taataacac caacgcaac cttccaacc cttgggtca gcgaggaca ccacgtact ggcaattga acagtccac tagtcgaag ttccgactg agaaacgcg tgaggcggt ggggcctat ccatggagg agctagttt cagatccga caggtttac aagagttta ctaattatt tgaggaagg agtacagcc acgaaaaca tcaggttct ggatggccg tgaatgatt taagacgtt ctgctcaat cccaaatcc agagaaaag ttaacgaaa tttttgagg aatttttgg cccctggtg ctccgtgtg agataaata tcgacggta aaaccatgg agaaagtcc accaagaga tgaaaacac ccagcaggt tcaccttcg acgctgcac aagaacatg tatatacat tgttgctga aaaaagatt gttatccta gatttatta ggtccgaat attataaga atctactgg cgacgggaa tccagcctt cccagaaaa agcgctttt tcggtttcg gccaaacca agaagaaaa cctcgacga gtagtgccc ccaaccagc aactgctgc agcagcagc cctcccagg gggcctcgg tgacttgcg gagccctgt cgaagcgca ggggcagcg accgcagtc tctcaggat cggcccacg agctcgccg tttccgggg tgaaagact ccaaggtgc cacactcac actcgcaga gtaacttga gcgacatcc cctacaggg gcgatttgg cgtgccttc cgaaagcca gtccggtgc caacatcgg ttaccagtc cagtgaaga agccaagca ttgcgatcg tggccggcg cctccacct cggaggagg tgtgtccgt gggagagcg gcgagcccc gttcccgga aaaactcgg cgcagctcg actccggca gctcctcgt cggacatca gcgtggcgg tggcggagg tctccgagc gggtccagc gcgtttgca ctctgactc agcaacaca ccgtcgacg gcagcagaa aactctcga cctccacca tggaccctc cccggagag catccgttt ccgtgccaa tcactcact caaccaccg gagactcct ccaagcgga aggtgtcct ccttcgagg acaacagct ccgccgcga ccagcacgt cgtcatcgg tgttcgtta ttcctagtg acacagaca ataaatctg aagaatgca ctagtacta ataaaactg ttgttaaga accacgggc ttgcaaagg cttgttcag tgacagggg cgaatgcac ctattatca gtgtcagtt cggtggccg acgatttgc cccccgata gcaaggagg agcaagtcg aacccacca ccagtcagg agccgctag cgccgctcg ctgagcccg acagtgaat cgccagcca gcgaactgc ctccttctg aacccatca ctgaagtcg aggccgaca cggaggcca agagtaacg acgtctgtc cctgggaag acgagtaag tgcaaacca gggccaagg ttttcgtga attggggca acaccacag agtgcacac cacctccca aactcccaa aataaataa aaattatat cccgaa
- >TC012518 RC
ttccgaatg caattttca ttacaatac gtgtgtatt ttcgtgtgc catttcgcg atcaataat ttcatgtcg agtcctggg aaagtcggg cgcgcgcac ctcttctct tcgccggat cgccattac agtcccaga tttcgctga gcgaagttg tctcgcgcg gcggaaacg cggctcggt acgcaacct gcatgcggg attaccact tccggtccc gttaacgca tccggttcg agccgttgg gacacgagg cggtgctaa ccgaccctc tcggtgtat ggtgtaaga tacagtggc ctcggatga agtccaacg cgcgatccg ctgttgttc gttgtatgt gggggtgag tgtcggcgt gatggggcg ggggcagag ctcgcccat ggagctcgg ctagaggcg tgcgcccct gcccggctg cccggcccc ccctctgcc gccctcacc acggcccac gcatggagc cggaccacc gggaccacc gatgccaca ggcccagcg cctttgaca aaatggagg ggttggtga gagagatgc aggacacgg agcacgggg gcgttccag ttcgaagcc aaaaattgt tccttacat ccattcccg ccgctttca tggggtacg acctaattg aatggctga tggagcgac tcgggatcg aagaatccg aggccctca atctagcca atcagctgt gtcagtatg gctactttt ttcccgtga gcgattcga aaaacctgg ttgtgaaag atgatagct cgctgtata ggtttcaat ctccgtatt attggcctt ggcagaaca gaccgccgg ataacatcg aatacgcga tttacttag cgaaaagaa ctttacgga ataagcaga ggcacggtc tggaggatt acgagttgg aagctttga gcaacttgc ggaaaaact tggcgaaca agtgggatt ttattacaa tgcaggctg aggagcagg tgaagctgt cgaaggtgc tgaagaagg gcgacaaga tcatcagcg actcgcagg agagggcgt actggcgcg tccacaggc ctccgccgg ggatggtca cctcgctgg agcagtgcc cggtgccga cgcgcagct ggaacggct ccaggaccc ggaaacgca ccatcgagg atggcaggc gagaagtcg agttgttga agaacagtc tgtcaagga ctagggtta aggtgtcgc aagcgctgg agagtatgg tccagcatg tcgagacct acgctgagt acgaccctt taataacac caacgcaac cttccaacc cttgggtca gcgaggaca ccacgtact ggcaattga acagtccac tagtcgaag ttccgactg agaaacgcg tgaggcggt ggggcctat ccatggagg agctagttt cagatccga caggtttac aagagttta ctaattatt tgaggaagg agtacagcc acgaaaaca tcaggttct ggatggccg tgaatgatt taagacgtt ctgctcaat cccaaatcc agagaaaag ttaacgaaa tttttgagg aatttttgg cccctggtg ctccgtgtg agataaata tcgacggta aaaccatgg agaaagtcc accaagaga tgaaaacac ccagcaggt tcaccttcg acgctgcac aagaacatg tatatacat tgttgctga aaaaagatt gttatccta gatttatta ggtccgaat attataaga atctactgg cgacgggaa tccagcctt cccagaaaa agcgctttt tcggtttcg gccaaacca agaagaaaa cctcgacga gtagtgccc ccaaccagc aactgctgc agcagcagc cctcccagg gggcctcgg tgacttgcg gagccctgt cgaagcgca ggggcagcg accgcagtc tctcaggat cggcccacg agctcgccg tttccgggg tgaaagact ccaaggtgc cacactcac actcgcaga gtaacttga gcgacatcc cctacagag tgaagaagc caagcattg cgatcgtgg ccggcgcct ccacctcgg aggaggtgt gtccgtggg agagcggcg agccccgtt cccggaaaa actcggcgc agctcgact ccggcagct cctcgtcgg acatcagcg tggcggtgg cggaggtct ccgagcggg tccagcgcg tttgcactc tgactcagc aacacaccg tcgacggca gcagaaaac tctcgacct ccaccatgg accctcccc ggagagcat ccgtttccg tgccaatca ctcactcaa ccaccggag actcctcca agcggaagg tgtcctcct tcgaggaca acagctccg ccgcgacca gcacgtcgt catcggtgt tcgttattc ctagtgaca cagacaata aatctgaag aatgcacta gtactaata aaactgttg ttaagaacc acgggcttg caaaggctt gttcagtga caggggcga atgcaccta ttatcagtg tcagttcgg tggccgacg atttgcccc ccgatagca aggaggagc aagtcgaac ccaccacca gtcaggagc cgctagcgc cgctcgctg agcccgaca gtgaatcgc cagccagcg aactgcctc cttctgaac ccatcactg aagtcgagg ccgacacgg aggccaaga gtaacgacg tctgtccct gggaagacg aggaaacgt gtaaggtgg acacgccct tcgtcaaga cttacgcta ctctgggct acctttagc taccgcaac tgtcaagag atcaagtga cgtttgctc acaacttct atttgattt tcttaattt tgtttcaag tttacctac ccaaagtta ggtattgtc aaaaacaag tttgattat gtcaggttt agcggggaa aaatgattc caaaggacg gttttaaga gtggaggtt caccagaga agggtcaaa ttttcaaac ggtttaggg cataacgga acggtaaca atgtcggca ttagaaata gactttctt gagcccttg gtcattatc tgatgttga cagcttcac gttccgccg tcccctact aaagtcagg aactgcacc tgattcttc tgtaccagt acactctca atataaata cgtggcatt gtcgtatta taatcaatg taactgtag gtgttgaaa aaaaataaa aacggatag ggttgatta acggtgcag ttatcgact ttccaatat ccacataaa aattgatgt ttgaacact ttcgtaagt aatcgagca caatattct cttaatgtc gtatcgttg tattagata tagagcatt taattatac gtattgtag cctagtggt gtatcaatg ttcagtgca gtttctaac tttcccaaa actttcggc caagtttcc aaaattcaa aatttttcg ttcgggttc gcgatttac aaaagggaa aatctcctt tggaatcat ctccttccg acgatgttt cagttccag tccccggaa taaattgcg attgtgagc aacacattt ccaagttaa cttaaatgt atccaggcc attttgatg caaattctg gcctatata cgtatttgt tgtttaata aacgtaggt tgactgtta ttgcttgac acaaaaatg ttaaaaaaa atctgatgg cctcattaa tatttgata gaagtacca gtggtgtat ataaaaccc tccgcctcc agctggggt ttcaattaa atactcaat taaagttaa cattatatt caaacatgc aatgttact tattgaagc tatttttat gataaatgt tttcgtctc ttagaatat tgtggtaag cgtcaaaat atatatgct gcctgacag tgagagagt tagtgatgt atatagttt tataataat agctattat aacgtatta ttaaaaatg ttaccactt aatgtccta aaacatttt atttcatag aattgttga taggcaatg ttattataa aattattgt agattaatg aagtaaaag aaatgtgat ttaatctc
The data is licensed under a
Creative Commons Attribution 3.0 Unported License. (CC-BY)
