Status
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Gene information
General information
TC012509 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 10555050..10563269
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012509
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05254

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012509 RA
      MLNNETDSF MNRVRRALT LPIRARCAE LTVLFDRGS 
      TLDLQNFFP LLIDNIFGP QGTVSWGLR VARDVNEDF 
      RQLEHFLSP CGPVFKLIY TLLKDPSAK YDFPVMYLP 
      VKVQQILEN AQLHPFYAD IVHVNPQTK HVISLLLNP 
      FDYYMFHFA YHLINPWHQ RSGSVLTTQ QQASWNTVY 
      YVLCCDYML HFLPTTPGA TVLPAIYYN GKNPIQPLQ 
      QMKSPVGSK LLNSSLLNK SVEEYSFPQ NLGRHPRNQ 
      IWRSETVLT VFIDMWLNN DQISQPTNQ INSSFNSSP 
      VPRLLQYTE LPTSEYMRI IRVLIKQLH AFSASAKID 
      DTHMCELKK IAIPMVEGK FYIFLRNLI HRWPLDGSF 
      RLLLELWLT YIQPWRYPP DATQADLKK QNLDAEDAM 
      TTAPPTVGR EYLPFIAEN LLIYVVIFQ QLLPRFTRV 
      DLVSPKMSV MLYRISKVF NQPNLASLL KEIEQSIEN 
      NHSPSHSYS SHWLSNLPP LNPTIPPAP EVYELVIQI 
      KRSISVAQE LVQIRKQEE QEHSVSFFD FLRIFYDTP 
      TTTDEFSLN ERLKVPEYL DKSLDHLKQ LFQLSEVPE 
      SEPDTSVLT NSGSSGLEI SNDFKFLTP ERVRERMKK 
      IKYEGDPDL HPIRTMEIA FLVRMLYQL SLQINENFR 
      QKFCQLYYD QTYWGRLSR QLLCPPLTI YKYDKTKPG 
      SPRVSMELP PRVSLRYFA SYHFWTYLM IGWIVAVTL 
      GYGLPVYLL FAFFIVLLY KCIRAIASN PVRHNYPTE 
      GFGNISFDD SF
    CDS sequence
    • >TC012509 RA
      atgttgaat aacgaaaca gacagtttt atgaatcgc 
      gtccggcga gccctgacg ctccctatc agagcccga 
      tgtgccgaa ctaaccgtg ttatttgat cggggaagc 
      accttagat ttgcagaat tttttcccc ctactaatc 
      gacaacata ttcgggccg caaggcaca gtaagttgg 
      ggcttacgt gtagccaga gatgttaat gaggatttc 
      cgacaactg gagcatttt ttatcgcct tgtggacct 
      gtctttaag ttaatttat acacttctc aaagatcca 
      tctgctaaa tacgatttt ccggtaatg tatttacct 
      gttaaggta caacagata ctggaaaat gcccagtta 
      cacccgttt tatgcagat attgttcat gttaatccg 
      cagaccaaa cacgtgatt tcattatta ttgaaccct 
      tttgattat tatatgttt cattttgcg tatcatttg 
      atcaatcct tggcaccaa agatcgggg tctgtttta 
      actacacaa caacaagcg tcgtggaat acggtttat 
      tatgtgtta tgctgcgat tatatgctt cacttttta 
      ccaaccact cctggggct acagtcttg cctgcgatt 
      tattataat gggaaaaat ccaatccag ccgctgcaa 
      caaatgaaa tctccagtt ggctcaaaa ctgctaaac 
      tcgtctctt ttaaataaa tcagttgag gagtattcg 
      tttccccaa aacttgggt cgccatccg cgaaaccaa 
      atatggagg agcgaaacg gttttgacc gttttcata 
      gacatgtgg ttgaataac gaccaaatc agccaaccg 
      accaatcaa attaacagt tcgtttaac tcttctcca 
      gttccgcga ttgttacaa tatacggaa ctcccaaca 
      tcggaatac atgcgaata atccgtgtt ttaataaag 
      caattgcat gcgttttca gcaagtgcc aaaatcgat 
      gacactcac atgtgtgaa ttgaaaaaa attgcgatt 
      ccaatggtt gagggaaaa ttttacatt tttttgcga 
      aacttgata cacaggtgg ccattggat ggctccttc 
      aggttactg ttagaactg tggttgact tatattcag 
      ccttggaga tatcctccc gatgccacg caagccgac 
      ctaaaaaaa caaaatctc gatgctgag gacgctatg 
      accaccgcg cctccaaca gttggtcgc gaatatttg 
      ccctttatt gcggaaaat ttactgatt tatgttgtg 
      atatttcaa cagttgctt cccaggttt actagagtg 
      gatctagtg agccctaaa atgtccgtc atgttgtac 
      agaattagt aaagtcttt aatcagccg aatttggcc 
      tcacttctc aaagaaatt gaacagtcg attgaaaat 
      aaccactcg ccttcgcac tcttacagc agccattgg 
      ctgtctaat ttaccgcct ttgaacccg accattccg 
      ccagctcca gaggtgtat gaattagtt atacaaatt 
      aagagatca atcagtgtg gcgcaagag ttggtacaa 
      ataagaaaa caggaggaa caagaacac agtgtcagc 
      tttttcgac tttctgaga attttttac gatacgcca 
      acaaccact gacgaattt agcttaaat gaacggttg 
      aaggttcct gaatatttg gataagtcc ctagaccac 
      ttgaagcaa ctgttccaa ttatcagag gtacccgaa 
      tccgagccg gacacatca gtgctcaca aattccggt 
      tcaagcggt ttggaaatt tccaatgat ttcaaattt 
      ctcactccg gagcgggtc agagaacga atgaagaaa 
      atcaaatat gaaggagac cccgattta catccgatc 
      cgaacgatg gaaatagca tttttagtt cgaatgttg 
      taccaactg tctcttcaa ataaacgaa aatttccgg 
      caaaagttc tgccaattg tactacgat cagacgtat 
      tggggacgt ttgtcgagg caacttttg tgccctcct 
      ctcacaatc tataaatat gataaaacc aaacctggc 
      tctccgagg gttagtatg gagttaccg ccgagagtt 
      agtttgagg tatttcgca agttatcac ttttggact 
      tatttaatg atcgggtgg attgttgcc gttacgtta 
      ggttatggt ttgccagtt tatttgtta tttgctttt 
      tttattgtt ctgttgtat aaatgtata agagccatc 
      gctagtaat cctgtcagg cataattat ccaacggaa 
      gggttcgga aatattagt ttcgatgac tctttttaa
    mRNA sequence
    • >TC012509 RA
      ttccgcttc tataaaagt gttattcta ggaacataa 
      cctataagt gtaaacaaa tcacccatt tcagaaaat 
      taggttatg ttaattgtt ggttaaaaa aaacgtttc 
      aaatttatt tactgtgtg tgtacttaa acgctaaaa 
      tgttgaata acgaaacag acagtttta tgaatcgcg 
      tccggcgag ccctgacgc tccctatca gagcccgat 
      gtgccgaac taaccgtgt tatttgatc ggggaagca 
      ccttagatt tgcagaatt ttttccccc tactaatcg 
      acaacatat tcgggccgc aaggcacag taagttggg 
      gcttacgtg tagccagag atgttaatg aggatttcc 
      gacaactgg agcattttt tatcgcctt gtggacctg 
      tctttaagt taatttata cacttctca aagatccat 
      ctgctaaat acgattttc cggtaatgt atttacctg 
      ttaaggtac aacagatac tggaaaatg cccagttac 
      acccgtttt atgcagata ttgttcatg ttaatccgc 
      agaccaaac acgtgattt cattattat tgaaccctt 
      ttgattatt atatgtttc attttgcgt atcatttga 
      tcaatcctt ggcaccaaa gatcggggt ctgttttaa 
      ctacacaac aacaagcgt cgtggaata cggtttatt 
      atgtgttat gctgcgatt atatgcttc actttttac 
      caaccactc ctggggcta cagtcttgc ctgcgattt 
      attataatg ggaaaaatc caatccagc cgctgcaac 
      aaatgaaat ctccagttg gctcaaaac tgctaaact 
      cgtctcttt taaataaat cagttgagg agtattcgt 
      ttccccaaa acttgggtc gccatccgc gaaaccaaa 
      tatggagga gcgaaacgg ttttgaccg ttttcatag 
      acatgtggt tgaataacg accaaatca gccaaccga 
      ccaatcaaa ttaacagtt cgtttaact cttctccag 
      ttccgcgat tgttacaat atacggaac tcccaacat 
      cggaataca tgcgaataa tccgtgttt taataaagc 
      aattgcatg cgttttcag caagtgcca aaatcgatg 
      acactcaca tgtgtgaat tgaaaaaaa ttgcgattc 
      caatggttg agggaaaat tttacattt ttttgcgaa 
      acttgatac acaggtggc cattggatg gctccttca 
      ggttactgt tagaactgt ggttgactt atattcagc 
      cttggagat atcctcccg atgccacgc aagccgacc 
      taaaaaaac aaaatctcg atgctgagg acgctatga 
      ccaccgcgc ctccaacag ttggtcgcg aatatttgc 
      cctttattg cggaaaatt tactgattt atgttgtga 
      tatttcaac agttgcttc ccaggttta ctagagtgg 
      atctagtga gccctaaaa tgtccgtca tgttgtaca 
      gaattagta aagtcttta atcagccga atttggcct 
      cacttctca aagaaattg aacagtcga ttgaaaata 
      accactcgc cttcgcact cttacagca gccattggc 
      tgtctaatt taccgcctt tgaacccga ccattccgc 
      cagctccag aggtgtatg aattagtta tacaaatta 
      agagatcaa tcagtgtgg cgcaagagt tggtacaaa 
      taagaaaac aggaggaac aagaacaca gtgtcagct 
      ttttcgact ttctgagaa ttttttacg atacgccaa 
      caaccactg acgaattta gcttaaatg aacggttga 
      aggttcctg aatatttgg ataagtccc tagaccact 
      tgaagcaac tgttccaat tatcagagg tacccgaat 
      ccgagccgg acacatcag tgctcacaa attccggtt 
      caagcggtt tggaaattt ccaatgatt tcaaatttc 
      tcactccgg agcgggtca gagaacgaa tgaagaaaa 
      tcaaatatg aaggagacc ccgatttac atccgatcc 
      gaacgatgg aaatagcat ttttagttc gaatgttgt 
      accaactgt ctcttcaaa taaacgaaa atttccggc 
      aaaagttct gccaattgt actacgatc agacgtatt 
      ggggacgtt tgtcgaggc aacttttgt gccctcctc 
      tcacaatct ataaatatg ataaaacca aacctggct 
      ctccgaggg ttagtatgg agttaccgc cgagagtta 
      gtttgaggt atttcgcaa gttatcact tttggactt 
      atttaatga tcgggtgga ttgttgccg ttacgttag 
      gttatggtt tgccagttt atttgttat ttgcttttt 
      ttattgttc tgttgtata aatgtataa gagccatcg 
      ctagtaatc ctgtcaggc ataattatc caacggaag 
      ggttcggaa atattagtt tcgatgact ctttttaaa 
      cgcttacat taataaaca ataaataaa tac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY