Status
Please wait ...
Gene information
General information
TC012507 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 9580755..9589316
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012507
    iBeetle screen iB_05253

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants strongly fragmented (cuticle crumbs) - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012507 RA
      MVDYYKVLE VSKNATTAE IKKAYRKLA 
      LKWHPDKNQ DNIEDATKK FKEISEAYE 
      VLSDDKKRR MYDQYGKEG LINGGSRSR 
      SRHDDEFDF GGFGFFTFR DPEDVFREF 
      FGGSTLADL FGDFHDHGH RDRRRNRHS 
      HPQNALSSS LFSPFGVSL GGSFMDEFF 
      NTGHGGFTS FSTINSTFT NGAGGTANV 
      KRTSTSTRF ANGKKITTK KVYENGRET 
      VMTYENDIL KSKTVNGVP QSLTYS
    CDS sequence
    • >TC012507 RA
      atggtggac tattacaaa gtcctcgag gtgtcaaaa 
      aacgcaacg actgctgaa attaagaaa gcttaccgc 
      aaattggct ttgaaatgg catcccgac aagaaccaa 
      gacaacatc gaggacgcc acaaagaag ttcaaagag 
      atctccgaa gcgtacgaa gttctttct gatgataag 
      aaacgtcgt atgtacgac cagtacggt aaagaaggg 
      ctcatcaac ggcggtagt cgtagtcgc agccgccac 
      gatgacgaa ttcgacttt ggcggtttc ggtttcttc 
      actttcaga gatccggag gacgttttc agggagttt 
      ttcgggggt tctactctt gccgacttg tttggagat 
      tttcacgat cacgggcat agagaccgg cgacggaac 
      agacattca catccacag aatgctctc agttcgagt 
      ctgttcagc ccgttcggg gtgagtctg gggggtagt 
      ttcatggac gagtttttc aatacgggc catggggga 
      ttcacctcc ttcagcacc ataaatagt acgtttaca 
      aacggggcg gggggcacg gccaacgtc aagaggacg 
      tcaacgtcg acgcgcttc gccaacggg aagaaaatc 
      accacgaag aaggtgtac gagaacggg agagagacg 
      gtgatgacg tacgagaac gatatcctc aaatcgaag 
      actgtgaat ggggttcct cagagtctg acgtacagt 
      taa
    mRNA sequence
    • >TC012507 RA
      tgcatcaaa tatcggctt ttgtccaca agcattgaa 
      gtcggctcg tgtgtgtgc gattgtttg gtcatagac 
      aactgataa atgtgcatg tgatattaa tcaacttgt 
      gcgtaaaag tgtgcgtta cagtgatgg atgatcgtg 
      ttaagtgat ctgtgattt atctatcgt taggaaaac 
      attttttcc cgtcctgct aagtaacct tccttaaag 
      ctggggcat aaggacgcc agtggcacc aaaatggtg 
      gactattac aaagtcctc gaggtgtca aaaaacgca 
      acgactgct gaaattaag aaagcttac cgcaaattg 
      gctttgaaa tggcatccc gacaagaac caagacaac 
      atcgaggac gccacaaag aagttcaaa gagatctcc 
      gaagcgtac gaagttctt tctgatgat aagaaacgt 
      cgtatgtac gaccagtac ggtaaagaa gggctcatc 
      aacggcggt agtcgtagt cgcagccgc cacgatgac 
      gaattcgac tttggcggt ttcggtttc ttcactttc 
      agagatccg gaggacgtt ttcagggag tttttcggg 
      ggttctact cttgccgac ttgtttgga gattttcac 
      gatcacggg catagagac cggcgacgg aacagacat 
      tcacatcca cagaatgct ctcagttcg agtctgttc 
      agcccgttc ggggtgagt ctggggggt agtttcatg 
      gacgagttt ttcaatacg ggccatggg ggattcacc 
      tccttcagc accataaat agtacgttt acaaacggg 
      gcggggggc acggccaac gtcaagagg acgtcaacg 
      tcgacgcgc ttcgccaac gggaagaaa atcaccacg 
      aagaaggtg tacgagaac gggagagag acggtgatg 
      acgtacgag aacgatatc ctcaaatcg aagactgtg 
      aatggggtt cctcagagt ctgacgtac agttaaaca 
      cactgctac ttacaaaat tagaagaat ctacaattt 
      atgtgatct tgatgttga tcgatttct ggccacgca 
      gtgttgcca aattgtcag tgtgtgtat taaagactt 
      cttgcaact ggtttcaag tttttttta ttcagttgt 
      acaataaaa tattatgat tctgttttc agaattttt 
      gtagtatct taattttta ataagatgt tatgatgat 
      gtaagaatc gaggaggtt tttacatca ttgcagttt 
      aggttagct agagatagg aattgatga cgctcttga 
      acgagaggt gctcaggat gagttgttt agttactag 
      ttacatttt tgtcatgtg aaaaagttc ggttttcgt 
      tttgttttt gccaagact gctcatttt ttattaagc 
      ttgattcaa aggaggaat taacatgga agagatatt 
      ttgtattat gcaacgttt taattttaa tttaatatt 
      aatttgcta gttttttcc caagcactt actattact 
      gttaagtac acaaatgat tatactatt aatgtgtgc 
      ttagagatg tacagtcac agtgttaga aaaaatata 
      atataaacg atttccgca t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY