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Gene information
General information
TC012499 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 9520175..9521979
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012499
    iBeetle screen iB_05252

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    appendages random remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva appendages shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs sometimes shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs podomer potentiallyfused - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012499 RA
      MLLSSSVLF VISFFLVLY FFKKLKCVS AKLYNLGKL 
      DGPSNHNII IGNVLRLHA APEVIFKKL RQWARDYYP 
      VYQLRAIHI PAACILAPE DFEAILSNP RHIEKSMVY 
      LLLHDWLGT GLLTSGGQK WATRRKILT PAFHFSILQ 
      QFITIFNEE TDKLVDTFK QQCDQPIEI TSHITQFTL 
      KTIGETAMG TKFEFTTRK QIEYKQAIL EIGEILLYR 
      LLNSWLISD YLNFFSPHR LREKRLVKT LHTFTRDVI 
      TEREKTFEK FQLPTEEHD VYIGKKRLA MLDLLLTAK 
      NEERTIDED GIREEVDTF MFEGHDTTS AALSFALML 
      LANHKNVQD QIVDEMVTV LGDLHQKPT YNNLQEMKY 
      LERAIKESL RLYPSVHFI SRKLGEDFV TCNGLKLPK 
      STITHLHIY DLHHNPDIY PDPEKFDPE RFRPENSQK 
      RHPFAYLPF SAGPRNCIG QKFAMLELK AAICGILAN 
      FILEPVDTP ESIVLVVDL VLRTKNGIK IGFIPRVK
    CDS sequence
    • >TC012499 RA
      atgttgcta tcatcatca gtgctcttt gtgatcagt 
      ttcttccta gttttgtat tttttcaaa aaactaaaa 
      tgcgtatca gcaaaatta tacaacctc ggtaaactc 
      gacggacct tcaaaccat aacataatt attggaaat 
      gtgttacgg cttcatgcg gctccagaa gtaattttt 
      aaaaaattg cgacaatgg gcacgtgac tactatcca 
      gtctaccaa ctgagagca attcatatc cctgcagca 
      tgtattttg gcaccggaa gactttgaa gccatttta 
      tccaatccg agacatatc gaaaaaagc atggtttat 
      ttgctttta cacgactgg ttaggaaca ggtcttcta 
      acaagtggt ggacaaaaa tgggcaaca agacgaaaa 
      atcctaaca ccagctttc cacttcagt atcctccaa 
      caattcatc acaatcttc aatgaagaa accgacaaa 
      cttgtcgac actttcaaa caacaatgc gaccaaccg 
      atcgagatc acctctcat attacacaa ttcacgttg 
      aaaacgatc ggtgaaact gcaatggga acaaaattc 
      gaattcaca acccgaaaa caaatcgaa tataaacaa 
      gcaatcctg gaaatcggc gaaatcctc ctctaccgc 
      ctcctcaac tcgtggctc atttccgac tacctcaat 
      ttcttcagt cctcaccgt ttgagggag aaacgcctc 
      gtcaaaacc ctccacact ttcactcga gacgtgatc 
      accgaacgt gaaaaaaca ttcgaaaaa ttccaacta 
      ccaacggaa gaacatgac gtttacatt ggcaaaaaa 
      cgactcgca atgctagac ctcctcctc acagccaaa 
      aacgaagaa agaaccatc gatgaagat ggaattcgt 
      gaggaagta gacactttc atgttcgaa ggccacgac 
      acgacttct gctgcccta agtttcgct ttgatgctt 
      ttggccaat cacaaaaac gtccaagac cagattgtg 
      gacgaaatg gtcacagtt ttgggcgac ctgcaccaa 
      aaaccgact tacaacaat ttgcaagaa atgaagtat 
      ctggaacga gcgataaaa gagagtttg agattgtac 
      ccaagcgtg cattttatt tcaaggaaa ttaggagag 
      gactttgtg acttgtaac ggcctcaaa ttgcctaaa 
      agtacaatc acgcatttg cacatctac gatctgcac 
      cacaatcct gacatttat cctgatcct gagaagttt 
      gatcctgag cgttttcgt cctgagaac tcccaaaaa 
      aggcatcct ttcgcctat ttgccattt agcgccggg 
      cctaggaac tgtataggc cagaaattt gcgatgttg 
      gagctgaaa gcggccatt tgtggcatt ttggccaat 
      tttatactt gaaccggtt gataccccc gaaagtatc 
      gtcttagtt gttgatctt gtgctccgc actaaaaac 
      gggataaaa atcggtttt attcccaga gttaaataa
    mRNA sequence
    • >TC012499 RA
      accgaaaaa attaactgt taatgtcaa aatgttgct 
      atcatcatc agtgctctt tgtgatcag tttcttcct 
      agttttgta ttttttcaa aaaactaaa atgcgtatc 
      agcaaaatt atacaacct cggtaaact cgacggacc 
      ttcaaacca taacataat tattggaaa tgtgttacg 
      gcttcatgc ggctccaga agtaatttt taaaaaatt 
      gcgacaatg ggcacgtga ctactatcc agtctacca 
      actgagagc aattcatat ccctgcagc atgtatttt 
      ggcaccgga agactttga agccatttt atccaatcc 
      gagacatat cgaaaaaag catggttta tttgctttt 
      acacgactg gttaggaac aggtcttct aacaagtgg 
      tggacaaaa atgggcaac aagacgaaa aatcctaac 
      accagcttt ccacttcag tatcctcca acaattcat 
      cacaatctt caatgaaga aaccgacaa acttgtcga 
      cactttcaa acaacaatg cgaccaacc gatcgagat 
      cacctctca tattacaca attcacgtt gaaaacgat 
      cggtgaaac tgcaatggg aacaaaatt cgaattcac 
      aacccgaaa acaaatcga atataaaca agcaatcct 
      ggaaatcgg cgaaatcct cctctaccg cctcctcaa 
      ctcgtggct catttccga ctacctcaa tttcttcag 
      tcctcaccg tttgaggga gaaacgcct cgtcaaaac 
      cctccacac tttcactcg agacgtgat caccgaacg 
      tgaaaaaac attcgaaaa attccaact accaacgga 
      agaacatga cgtttacat tggcaaaaa acgactcgc 
      aatgctaga cctcctcct cacagccaa aaacgaaga 
      aagaaccat cgatgaaga tggaattcg tgaggaagt 
      agacacttt catgttcga aggccacga cacgacttc 
      tgctgccct aagtttcgc tttgatgct tttggccaa 
      tcacaaaaa cgtccaaga ccagattgt ggacgaaat 
      ggtcacagt tttgggcga cctgcacca aaaaccgac 
      ttacaacaa tttgcaaga aatgaagta tctggaacg 
      agcgataaa agagagttt gagattgta cccaagcgt 
      gcattttat ttcaaggaa attaggaga ggactttgt 
      gacttgtaa cggcctcaa attgcctaa aagtacaat 
      cacgcattt gcacatcta cgatctgca ccacaatcc 
      tgacattta tcctgatcc tgagaagtt tgatcctga 
      gcgttttcg tcctgagaa ctcccaaaa aaggcatcc 
      tttcgccta tttgccatt tagcgccgg gcctaggaa 
      ctgtatagg ccagaaatt tgcgatgtt ggagctgaa 
      agcggccat ttgtggcat tttggccaa ttttatact 
      tgaaccggt tgatacccc cgaaagtat cgtcttagt 
      tgttgatct tgtgctccg cactaaaaa cgggataaa 
      aatcggttt tattcccag agttaaata aatatttac 
      aaagaactg ttgcatttc aattattac caaatcttg 
      acctttgtt tttgtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY