Status
Please wait ...
Gene information
General information
TC012499 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 9520175..9521979
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC012499
    iBeetle screen iB_05252

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    appendages random remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva appendages shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs sometimes shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs podomer potentiallyfused - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012499 RA
      MLLSSSVLF VISFFLVLY FFKKLKCVS AKLYNLGKL 
DGPSNHNII IGNVLRLHA APEVIFKKL RQWARDYYP 
VYQLRAIHI PAACILAPE DFEAILSNP RHIEKSMVY 
LLLHDWLGT GLLTSGGQK WATRRKILT PAFHFSILQ 
QFITIFNEE TDKLVDTFK QQCDQPIEI TSHITQFTL 
KTIGETAMG TKFEFTTRK QIEYKQAIL EIGEILLYR 
LLNSWLISD YLNFFSPHR LREKRLVKT LHTFTRDVI 
TEREKTFEK FQLPTEEHD VYIGKKRLA MLDLLLTAK 
NEERTIDED GIREEVDTF MFEGHDTTS AALSFALML 
LANHKNVQD QIVDEMVTV LGDLHQKPT YNNLQEMKY 
LERAIKESL RLYPSVHFI SRKLGEDFV TCNGLKLPK 
STITHLHIY DLHHNPDIY PDPEKFDPE RFRPENSQK 
RHPFAYLPF SAGPRNCIG QKFAMLELK AAICGILAN 
FILEPVDTP ESIVLVVDL VLRTKNGIK IGFIPRVK
    CDS sequence
    • >TC012499 RA
      atgttgcta tcatcatca gtgctcttt gtgatcagt 
ttcttccta gttttgtat tttttcaaa aaactaaaa 
tgcgtatca gcaaaatta tacaacctc ggtaaactc 
gacggacct tcaaaccat aacataatt attggaaat 
gtgttacgg cttcatgcg gctccagaa gtaattttt 
aaaaaattg cgacaatgg gcacgtgac tactatcca 
gtctaccaa ctgagagca attcatatc cctgcagca 
tgtattttg gcaccggaa gactttgaa gccatttta 
tccaatccg agacatatc gaaaaaagc atggtttat 
ttgctttta cacgactgg ttaggaaca ggtcttcta 
acaagtggt ggacaaaaa tgggcaaca agacgaaaa 
atcctaaca ccagctttc cacttcagt atcctccaa 
caattcatc acaatcttc aatgaagaa accgacaaa 
cttgtcgac actttcaaa caacaatgc gaccaaccg 
atcgagatc acctctcat attacacaa ttcacgttg 
aaaacgatc ggtgaaact gcaatggga acaaaattc 
gaattcaca acccgaaaa caaatcgaa tataaacaa 
gcaatcctg gaaatcggc gaaatcctc ctctaccgc 
ctcctcaac tcgtggctc atttccgac tacctcaat 
ttcttcagt cctcaccgt ttgagggag aaacgcctc 
gtcaaaacc ctccacact ttcactcga gacgtgatc 
accgaacgt gaaaaaaca ttcgaaaaa ttccaacta 
ccaacggaa gaacatgac gtttacatt ggcaaaaaa 
cgactcgca atgctagac ctcctcctc acagccaaa 
aacgaagaa agaaccatc gatgaagat ggaattcgt 
gaggaagta gacactttc atgttcgaa ggccacgac 
acgacttct gctgcccta agtttcgct ttgatgctt 
ttggccaat cacaaaaac gtccaagac cagattgtg 
gacgaaatg gtcacagtt ttgggcgac ctgcaccaa 
aaaccgact tacaacaat ttgcaagaa atgaagtat 
ctggaacga gcgataaaa gagagtttg agattgtac 
ccaagcgtg cattttatt tcaaggaaa ttaggagag 
gactttgtg acttgtaac ggcctcaaa ttgcctaaa 
agtacaatc acgcatttg cacatctac gatctgcac 
cacaatcct gacatttat cctgatcct gagaagttt 
gatcctgag cgttttcgt cctgagaac tcccaaaaa 
aggcatcct ttcgcctat ttgccattt agcgccggg 
cctaggaac tgtataggc cagaaattt gcgatgttg 
gagctgaaa gcggccatt tgtggcatt ttggccaat 
tttatactt gaaccggtt gataccccc gaaagtatc 
gtcttagtt gttgatctt gtgctccgc actaaaaac 
gggataaaa atcggtttt attcccaga gttaaataa
    mRNA sequence
    • >TC012499 RA
      accgaaaaa attaactgt taatgtcaa aatgttgct 
atcatcatc agtgctctt tgtgatcag tttcttcct 
agttttgta ttttttcaa aaaactaaa atgcgtatc 
agcaaaatt atacaacct cggtaaact cgacggacc 
ttcaaacca taacataat tattggaaa tgtgttacg 
gcttcatgc ggctccaga agtaatttt taaaaaatt 
gcgacaatg ggcacgtga ctactatcc agtctacca 
actgagagc aattcatat ccctgcagc atgtatttt 
ggcaccgga agactttga agccatttt atccaatcc 
gagacatat cgaaaaaag catggttta tttgctttt 
acacgactg gttaggaac aggtcttct aacaagtgg 
tggacaaaa atgggcaac aagacgaaa aatcctaac 
accagcttt ccacttcag tatcctcca acaattcat 
cacaatctt caatgaaga aaccgacaa acttgtcga 
cactttcaa acaacaatg cgaccaacc gatcgagat 
cacctctca tattacaca attcacgtt gaaaacgat 
cggtgaaac tgcaatggg aacaaaatt cgaattcac 
aacccgaaa acaaatcga atataaaca agcaatcct 
ggaaatcgg cgaaatcct cctctaccg cctcctcaa 
ctcgtggct catttccga ctacctcaa tttcttcag 
tcctcaccg tttgaggga gaaacgcct cgtcaaaac 
cctccacac tttcactcg agacgtgat caccgaacg 
tgaaaaaac attcgaaaa attccaact accaacgga 
agaacatga cgtttacat tggcaaaaa acgactcgc 
aatgctaga cctcctcct cacagccaa aaacgaaga 
aagaaccat cgatgaaga tggaattcg tgaggaagt 
agacacttt catgttcga aggccacga cacgacttc 
tgctgccct aagtttcgc tttgatgct tttggccaa 
tcacaaaaa cgtccaaga ccagattgt ggacgaaat 
ggtcacagt tttgggcga cctgcacca aaaaccgac 
ttacaacaa tttgcaaga aatgaagta tctggaacg 
agcgataaa agagagttt gagattgta cccaagcgt 
gcattttat ttcaaggaa attaggaga ggactttgt 
gacttgtaa cggcctcaa attgcctaa aagtacaat 
cacgcattt gcacatcta cgatctgca ccacaatcc 
tgacattta tcctgatcc tgagaagtt tgatcctga 
gcgttttcg tcctgagaa ctcccaaaa aaggcatcc 
tttcgccta tttgccatt tagcgccgg gcctaggaa 
ctgtatagg ccagaaatt tgcgatgtt ggagctgaa 
agcggccat ttgtggcat tttggccaa ttttatact 
tgaaccggt tgatacccc cgaaagtat cgtcttagt 
tgttgatct tgtgctccg cactaaaaa cgggataaa 
aatcggttt tattcccag agttaaata aatatttac 
aaagaactg ttgcatttc aattattac caaatcttg 
acctttgtt tttgtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY