GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_05252 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
remarks: muscle pattern potentially obscured by segmentation defect,
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%
cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
appendages random remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
larva appendages shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
head shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
legs sometimes shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
legs podomer potentiallyfused - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
- >TC012499 RA
MLLSSSVLF VISFFLVLY FFKKLKCVS AKLYNLGKL DGPSNHNII IGNVLRLHA APEVIFKKL RQWARDYYP VYQLRAIHI PAACILAPE DFEAILSNP RHIEKSMVY LLLHDWLGT GLLTSGGQK WATRRKILT PAFHFSILQ QFITIFNEE TDKLVDTFK QQCDQPIEI TSHITQFTL KTIGETAMG TKFEFTTRK QIEYKQAIL EIGEILLYR LLNSWLISD YLNFFSPHR LREKRLVKT LHTFTRDVI TEREKTFEK FQLPTEEHD VYIGKKRLA MLDLLLTAK NEERTIDED GIREEVDTF MFEGHDTTS AALSFALML LANHKNVQD QIVDEMVTV LGDLHQKPT YNNLQEMKY LERAIKESL RLYPSVHFI SRKLGEDFV TCNGLKLPK STITHLHIY DLHHNPDIY PDPEKFDPE RFRPENSQK RHPFAYLPF SAGPRNCIG QKFAMLELK AAICGILAN FILEPVDTP ESIVLVVDL VLRTKNGIK IGFIPRVK
- >TC012499 RA
atgttgcta tcatcatca gtgctcttt gtgatcagt ttcttccta gttttgtat tttttcaaa aaactaaaa tgcgtatca gcaaaatta tacaacctc ggtaaactc gacggacct tcaaaccat aacataatt attggaaat gtgttacgg cttcatgcg gctccagaa gtaattttt aaaaaattg cgacaatgg gcacgtgac tactatcca gtctaccaa ctgagagca attcatatc cctgcagca tgtattttg gcaccggaa gactttgaa gccatttta tccaatccg agacatatc gaaaaaagc atggtttat ttgctttta cacgactgg ttaggaaca ggtcttcta acaagtggt ggacaaaaa tgggcaaca agacgaaaa atcctaaca ccagctttc cacttcagt atcctccaa caattcatc acaatcttc aatgaagaa accgacaaa cttgtcgac actttcaaa caacaatgc gaccaaccg atcgagatc acctctcat attacacaa ttcacgttg aaaacgatc ggtgaaact gcaatggga acaaaattc gaattcaca acccgaaaa caaatcgaa tataaacaa gcaatcctg gaaatcggc gaaatcctc ctctaccgc ctcctcaac tcgtggctc atttccgac tacctcaat ttcttcagt cctcaccgt ttgagggag aaacgcctc gtcaaaacc ctccacact ttcactcga gacgtgatc accgaacgt gaaaaaaca ttcgaaaaa ttccaacta ccaacggaa gaacatgac gtttacatt ggcaaaaaa cgactcgca atgctagac ctcctcctc acagccaaa aacgaagaa agaaccatc gatgaagat ggaattcgt gaggaagta gacactttc atgttcgaa ggccacgac acgacttct gctgcccta agtttcgct ttgatgctt ttggccaat cacaaaaac gtccaagac cagattgtg gacgaaatg gtcacagtt ttgggcgac ctgcaccaa aaaccgact tacaacaat ttgcaagaa atgaagtat ctggaacga gcgataaaa gagagtttg agattgtac ccaagcgtg cattttatt tcaaggaaa ttaggagag gactttgtg acttgtaac ggcctcaaa ttgcctaaa agtacaatc acgcatttg cacatctac gatctgcac cacaatcct gacatttat cctgatcct gagaagttt gatcctgag cgttttcgt cctgagaac tcccaaaaa aggcatcct ttcgcctat ttgccattt agcgccggg cctaggaac tgtataggc cagaaattt gcgatgttg gagctgaaa gcggccatt tgtggcatt ttggccaat tttatactt gaaccggtt gataccccc gaaagtatc gtcttagtt gttgatctt gtgctccgc actaaaaac gggataaaa atcggtttt attcccaga gttaaataa
- >TC012499 RA
accgaaaaa attaactgt taatgtcaa aatgttgct atcatcatc agtgctctt tgtgatcag tttcttcct agttttgta ttttttcaa aaaactaaa atgcgtatc agcaaaatt atacaacct cggtaaact cgacggacc ttcaaacca taacataat tattggaaa tgtgttacg gcttcatgc ggctccaga agtaatttt taaaaaatt gcgacaatg ggcacgtga ctactatcc agtctacca actgagagc aattcatat ccctgcagc atgtatttt ggcaccgga agactttga agccatttt atccaatcc gagacatat cgaaaaaag catggttta tttgctttt acacgactg gttaggaac aggtcttct aacaagtgg tggacaaaa atgggcaac aagacgaaa aatcctaac accagcttt ccacttcag tatcctcca acaattcat cacaatctt caatgaaga aaccgacaa acttgtcga cactttcaa acaacaatg cgaccaacc gatcgagat cacctctca tattacaca attcacgtt gaaaacgat cggtgaaac tgcaatggg aacaaaatt cgaattcac aacccgaaa acaaatcga atataaaca agcaatcct ggaaatcgg cgaaatcct cctctaccg cctcctcaa ctcgtggct catttccga ctacctcaa tttcttcag tcctcaccg tttgaggga gaaacgcct cgtcaaaac cctccacac tttcactcg agacgtgat caccgaacg tgaaaaaac attcgaaaa attccaact accaacgga agaacatga cgtttacat tggcaaaaa acgactcgc aatgctaga cctcctcct cacagccaa aaacgaaga aagaaccat cgatgaaga tggaattcg tgaggaagt agacacttt catgttcga aggccacga cacgacttc tgctgccct aagtttcgc tttgatgct tttggccaa tcacaaaaa cgtccaaga ccagattgt ggacgaaat ggtcacagt tttgggcga cctgcacca aaaaccgac ttacaacaa tttgcaaga aatgaagta tctggaacg agcgataaa agagagttt gagattgta cccaagcgt gcattttat ttcaaggaa attaggaga ggactttgt gacttgtaa cggcctcaa attgcctaa aagtacaat cacgcattt gcacatcta cgatctgca ccacaatcc tgacattta tcctgatcc tgagaagtt tgatcctga gcgttttcg tcctgagaa ctcccaaaa aaggcatcc tttcgccta tttgccatt tagcgccgg gcctaggaa ctgtatagg ccagaaatt tgcgatgtt ggagctgaa agcggccat ttgtggcat tttggccaa ttttatact tgaaccggt tgatacccc cgaaagtat cgtcttagt tgttgatct tgtgctccg cactaaaaa cgggataaa aatcggttt tattcccag agttaaata aatatttac aaagaactg ttgcatttc aattattac caaatcttg acctttgtt tttgtt
