Status
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Gene information
General information
TC034362
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC012494,TC012493
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 9464642..9496607
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034362
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05251

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection



    pupal molt not completely molted - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034362 RA
      MRNSTNIFL VNLSVADLM VLLVCTPTV LVEVNSRPE 
      TWVLGREMC KAVPFVELT VAHASVLTI LAISFERYY 
      AICKPLKAG YICTKTRAS LICLLAWFI AALFTSPIL 
      AITQYGPEE YVDGSIVFV CFSLVEDTL PCVFFLGSI 
      LIFFIFPLA ILIIVYILI AKTLMHHPV AMTGTKTVM 
      PSQSAIKYR KQVILMLGT VVLAFFICL LPFRALTFW 
      IIVAPAGSN FEIGFENYY NILYFSRIM FHINSAVNP 
      ILYNIISSK FRSGFFKLC GMKIVKKRR KDKREITRK 
      STSSSTHTS SQQTSDSFL SKRNSEKKC GSLREIKEV 
      SDCDSSESR KEVYVRVPL GQRFSNGEI FV
    • >TC034362 RB
      MRNSTNIFL VNLSVADLM VLLVCTPTV LVEVNSRPE 
      TWVLGREMC KAVPFVELT VAHASVLTI LAISFERYY 
      AICKPLKAG YICTKTRAS LICLLAWFI AALFTSPML 
      VIAEYKQLD YFDGSKVPA CHTLANTFW SALYFLTII 
      FLFFIIPLI ILLILYCII AKNLMSNAA TLVLNKHID 
      NISLRARKQ VVLMLGTVV LSFFLCLIP FRVFILWII 
      LVPEEQVYH LEIEKYYNI LYFCRIMVY LNSAINPIL 
      YNLMSSKFR TGFIICSES NRKFYFKRA RNGTFSTTA 
      NSCRSSTFR NNRDEYNVC FRPRNNSIL IKNFNDSPE 
      SNRSNEIDG PEK
    CDS sequence
    • >TC034362 RA
      atgaggaac agcacgaat atattttta gtaaactta 
      agtgttgct gatttaatg gtgctcctg gtatgcaca 
      cccaccgtc ctcgtcgag gtcaactcg cgacccgaa 
      acctgggtc ctcggcagg gaaatgtgc aaagccgtg 
      ccctttgtt gaactcacc gtggcccac gcgtcggtc 
      ctcactatc ctggccata agtttcgaa cgttattac 
      gccatctgc aagcccctc aaggccgga tacatatgc 
      accaaaacg agagcatcc cttatctgc ttgttagcc 
      tggttcatc gctgcctta ttcactagc ccgatcttg 
      gcaattacc cagtacgga cctgaggag tacgtcgat 
      ggctcgatt gtcttcgtt tgcttcagt ctagtcgaa 
      gacaccctc ccttgcgtc tttttcctc ggctcgatc 
      ctcattttc ttcattttc cctctcgcc atcctaatt 
      atcgtctat atactaatt gccaagact ctaatgcac 
      caccctgtg gccatgacg ggcaccaag accgtcatg 
      ccgtcccaa agcgccatc aagtaccga aaacaagtc 
      attttgatg ctggggacg gtcgttttg gccttcttt 
      atctgtttg ctccctttt cgagcgctg accttttgg 
      atcatcgta gccccagct gggtccaac ttcgaaatc 
      gggttcgaa aactactac aatattctc tatttctcc 
      cggattatg tttcacata aactcggcc gtaaacccg 
      attttgtac aatataatc agttcgaaa tttcggagc 
      ggctttttt aaactatgc ggcatgaaa atcgtcaag 
      aagagaaga aaagacaag cgcgaaata actcgaaaa 
      agcacgagt tcgtcgact cacacttcc tctcaacaa 
      acttccgac tcgtttctt agtaagagg aattcggag 
      aaaaaatgc ggcagtctc agggaaatc aaagaagtg 
      tccgattgc gacagttcg gagagccgg aaagaggtt 
      tacgtgaga gtccccctg gggcagagg ttctcaaac 
      ggggagatt ttcgtctag
    • >TC034362 RB
      atgaggaac agcacgaat atattttta gtaaactta 
      agtgttgct gatttaatg gtgctcctg gtatgcaca 
      cccaccgtc ctcgtcgag gtcaactcg cgacccgaa 
      acctgggtc ctcggcagg gaaatgtgc aaagccgtg 
      ccctttgtt gaactcacc gtggcccac gcgtcggtc 
      ctcactatc ctggccata agtttcgaa cgttattac 
      gccatctgc aagcccctc aaggccgga tacatatgc 
      accaaaacg agagcatcc cttatctgc ttgttagcc 
      tggttcatc gctgcctta ttcactagc ccaatgctt 
      gtcatagcc gaatacaag caacttgat tacttcgac 
      ggttccaag gtaccagct tgtcacacg ttggccaat 
      acgttttgg tccgccctc tacttcctc accataatc 
      ttcctcttc ttcataatc cctctcata atccttctc 
      atcctgtac tgtataatc gccaaaaat ctcatgtcg 
      aacgcagca acgctagtc ctcaacaaa cacatcgat 
      aatatctcg ttgcgggcc agaaaacag gtggttctg 
      atgttggga accgtagtg ttgagcttc tttctgtgt 
      ttgatcccg ttccgggtg tttatcctc tggatcatc 
      ctagttccg gaagagcaa gtctatcac ttagaaatc 
      gagaaatac tacaacata ttgtatttc tgcaggatt 
      atggtgtat ctaaattcg gcgatcaat ccgattctg 
      tacaatctg atgagttcg aaattccgg accgggttt 
      attatttgt tcggagtcg aatcgaaag ttttacttt 
      aaaagggcc agaaatggc acttttagc acgacggcc 
      aactcgtgt cggagtagt acgttcagg aataatcgt 
      gacgagtat aatgtgtgt ttcaggccg agaaataac 
      tcaattctt atcaaaaat ttcaacgat tcgccggaa 
      tcgaaccga agtaacgaa attgacgga ccggaaaaa 
      taa
    mRNA sequence
    • >TC034362 RA
      gtccaattg gatgtcacc tattattgc gaattaaaa 
      aatccaagt catcgaaaa tcgtagaga attgagcgg 
      ttttcaagc cagccaagc gatttgaaa gcttggcgt 
      ttgcgccaa caaaagcag gaaccgttt ggccggcca 
      catatctat ataggtagt atcttggga gaatgaaac 
      cggcgcttc gtcgctcta cccatgctc tttgccagt 
      ctgttttca aaagccgtc aacagagca ctgaggagt 
      cggtgaaaa tttccgaaa ctcgatgtc acaccacag 
      ccgaacggt ccgttgaaa aaacacccg cgagatttc 
      ctgcgcaat catgacaca caaaaatta tccaaacaa 
      ataaccgtt tgaggcaac gagacagtt tcacaatcg 
      aaatgacac gtgaaaaag ccgaaaata cgaacgagg 
      tgtttatgt tacggtgaa acgtgcgag aagatcccg 
      cgatggaat cacaatcag ctattttct cccggaata 
      tgaatctta gtaaaatgg agactgaaa gtagtgata 
      actgacccc caatcctac tatgatatg atttcgtca 
      aaattcagc ccttcgaca acgaccacg acgacgact 
      acatcaagt gctctgaac tcaacacct ctgattgaa 
      tttaacata agcacatcg aattatctc tatacacca 
      tcgtcgacg atagattta tacaacaca tcgagcgtt 
      tcgaacttc aacgagtca tcatcatca gtgttcccc 
      aattatatc cggacgacg tcgatggtt ttctgtata 
      ataataatg tgtttgggt gtaatcgga aatgtaatg 
      gtgcctatc gttattttt aaaactaag gatatgagg 
      aacagcacg aatatattt ttagtaaac ttaagtgtt 
      gctgattta atggtgctc ctggtatgc acacccacc 
      gtcctcgtc gaggtcaac tcgcgaccc gaaacctgg 
      gtcctcggc agggaaatg tgcaaagcc gtgcccttt 
      gttgaactc accgtggcc cacgcgtcg gtcctcact 
      atcctggcc ataagtttc gaacgttat tacgccatc 
      tgcaagccc ctcaaggcc ggatacata tgcaccaaa 
      acgagagca tcccttatc tgcttgtta gcctggttc 
      atcgctgcc ttattcact agcccgatc ttggcaatt 
      acccagtac ggacctgag gagtacgtc gatggctcg 
      attgtcttc gtttgcttc agtctagtc gaagacacc 
      ctcccttgc gtctttttc ctcggctcg atcctcatt 
      ttcttcatt ttccctctc gccatccta attatcgtc 
      tatatacta attgccaag actctaatg caccaccct 
      gtggccatg acgggcacc aagaccgtc atgccgtcc 
      caaagcgcc atcaagtac cgaaaacaa gtcattttg 
      atgctgggg acggtcgtt ttggccttc tttatctgt 
      ttgctccct tttcgagcg ctgaccttt tggatcatc 
      gtagcccca gctgggtcc aacttcgaa atcgggttc 
      gaaaactac tacaatatt ctctatttc tcccggatt 
      atgtttcac ataaactcg gccgtaaac ccgattttg 
      tacaatata atcagttcg aaatttcgg agcggcttt 
      tttaaacta tgcggcatg aaaatcgtc aagaagaga 
      agaaaagac aagcgcgaa ataactcga aaaagcacg 
      agttcgtcg actcacact tcctctcaa caaacttcc 
      gactcgttt cttagtaag aggaattcg gagaaaaaa 
      tgcggcagt ctcagggaa atcaaagaa gtgtccgat 
      tgcgacagt tcggagagc cggaaagag gtttacgtg 
      agagtcccc ctggggcag aggttctca aacggggag 
      attttcgtc tagagcaca aaagactac aacagatca 
      tatattttg gtacttttt attcgagaa aaggactga 
      tttttgggg acttgtcaa ttctgagta ggtacccac 
      caacgacaa tatgtgttc tttttgtct tgtcatcaa 
      ttattatat cggattatt tatttattt agagtaggt 
      gtgtttgaa cgggccttg ctgtatact gtgtgggaa 
      ttaccgagg tgggagcga gggagcttt tggattttt 
      aatcaaaat ctaagcgtg taatggccg agaaaccat 
      tacggagat actacattc gtttttatt acacggcct 
      ttctgtatt tattaataa aaaataaat gtgtttcaa 
      atta
    • >TC034362 RB
      gtccaattg gatgtcacc tattattgc gaattaaaa 
      aatccaagt catcgaaaa tcgtagaga attgagcgg 
      ttttcaagc cagccaagc gatttgaaa gcttggcgt 
      ttgcgccaa caaaagcag gaaccgttt ggccggcca 
      catatctat ataggtagt atcttggga gaatgaaac 
      cggcgcttc gtcgctcta cccatgctc tttgccagt 
      ctgttttca aaagccgtc aacagagca ctgaggagt 
      cggtgaaaa tttccgaaa ctcgatgtc acaccacag 
      ccgaacggt ccgttgaaa aaacacccg cgagatttc 
      ctgcgcaat catgacaca caaaaatta tccaaacaa 
      ataaccgtt tgaggcaac gagacagtt tcacaatcg 
      aaatgacac gtgaaaaag ccgaaaata cgaacgagg 
      tgtttatgt tacggtgaa acgtgcgag aagatcccg 
      cgatggaat cacaatcag ctattttct cccggaata 
      tgaatctta gtaaaatgg agactgaaa gtagtgata 
      actgacccc caatcctac tatgatatg atttcgtca 
      aaattcagc ccttcgaca acgaccacg acgacgact 
      acatcaagt gctctgaac tcaacacct ctgattgaa 
      tttaacata agcacatcg aattatctc tatacacca 
      tcgtcgacg atagattta tacaacaca tcgagcgtt 
      tcgaacttc aacgagtca tcatcatca gtgttcccc 
      aattatatc cggacgacg tcgatggtt ttctgtata 
      ataataatg tgtttgggt gtaatcgga aatgtaatg 
      gtgcctatc gttattttt aaaactaag gatatgagg 
      aacagcacg aatatattt ttagtaaac ttaagtgtt 
      gctgattta atggtgctc ctggtatgc acacccacc 
      gtcctcgtc gaggtcaac tcgcgaccc gaaacctgg 
      gtcctcggc agggaaatg tgcaaagcc gtgcccttt 
      gttgaactc accgtggcc cacgcgtcg gtcctcact 
      atcctggcc ataagtttc gaacgttat tacgccatc 
      tgcaagccc ctcaaggcc ggatacata tgcaccaaa 
      acgagagca tcccttatc tgcttgtta gcctggttc 
      atcgctgcc ttattcact agcccaatg cttgtcata 
      gccgaatac aagcaactt gattacttc gacggttcc 
      aaggtacca gcttgtcac acgttggcc aatacgttt 
      tggtccgcc ctctacttc ctcaccata atcttcctc 
      ttcttcata atccctctc ataatcctt ctcatcctg 
      tactgtata atcgccaaa aatctcatg tcgaacgca 
      gcaacgcta gtcctcaac aaacacatc gataatatc 
      tcgttgcgg gccagaaaa caggtggtt ctgatgttg 
      ggaaccgta gtgttgagc ttctttctg tgtttgatc 
      ccgttccgg gtgtttatc ctctggatc atcctagtt 
      ccggaagag caagtctat cacttagaa atcgagaaa 
      tactacaac atattgtat ttctgcagg attatggtg 
      tatctaaat tcggcgatc aatccgatt ctgtacaat 
      ctgatgagt tcgaaattc cggaccggg tttattatt 
      tgttcggag tcgaatcga aagttttac tttaaaagg 
      gccagaaat ggcactttt agcacgacg gccaactcg 
      tgtcggagt agtacgttc aggaataat cgtgacgag 
      tataatgtg tgtttcagg ccgagaaat aactcaatt 
      cttatcaaa aatttcaac gattcgccg gaatcgaac 
      cgaagtaac gaaattgac ggaccggaa aaataattc 
      cactatttt tagaccgga caatggtct atttttatt 
      tatgttttt gtagaaaaa tatacgttt atttataga 
      cagggccac gcagtggcg taaccattc tgacttgaa 
      cgggggcag aattttttg aaatattga tcgttgcgg 
      aaaccagaa catttagac ttgaaacga gtataagta 
      cgagtacga gaaaatgtt tattttaaa aaagtatac 
      attaatatt acagggtat gatttaaaa aaaactgac 
      atgcgctgt agaattttt ttatggcca gatttaaaa 
      aaaattata ataaatcaa atggctttt a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY