| General information | |||
| TC012483 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 9113381..9130278 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC012483 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Reference | * {{ blurReferenceResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05248 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC012483 RA
MFDCELDED TPFAVVTVP NMAASYGKK NGIWYAAPG GQGWSKATS RKASIAETD APPPGGGTI KPSSGRVIR IINNMDHTV QCRVLLNLR TSQPFEEVL EDLGQVLKM NGAKRMYTV SGQEVRSFS QLRNEFADV DTFYLGVGS VGGGVGGVM LSPVRRARS RAPQTAIVE DMSKQRRAR SKSRPRALY APESEIVRT NDYTLLEVL KEEPVRVTI KGLRRTFYP PIHHAPMDN TPPEKKMQL DYGYRGTDS RRNLWVLPT GELLYFVAA VAVMYDRDE EAQRHYTGH TEDIQCMDL HPSREMVAS GQRAGRNRK TQAHIRIWS TETLQTLYV FGMGEFEVG VAAVAFSQL NGGSYVLAV DGGRESILS VWQWQWGHL LGKVATLQE ELTGAVFHP LDDNLMITH GKGHLTFWN RRKDGFFER TDIIKPPSR TLITALQFE QDGDVITAD SDGFITIYS VDSDGAYFV RLEYEAHNK GVSSLVMLS EGTLLSGGD KDRKIVAWD SLQNYKKIT ETKLAESAG GVRSIYPQR PGRNDGNIY VGTTKNNIL EGSLQRRFN QVVFGHGRQ LWGLAVHPD DEVFATAGH DKNIALWRK NKFIWTLQV AFECISLSF HPFGVALAA GSTEGHLII INTETGTLI NTIRVCGSP LSCVGYNPT GDMIAIASQ NGSIYLFRV SRDGFSYKK SNKIRGSQP LIQMDWSTD SNFLQTVTA DYDLCFWDV KSLSPEKSP IAMKDVKWY TQNSTVGFL VAGIWNNRF YPMTSIIST VSRSAAHDL LISGDTDGY IRLFRYPCL SPKAEYNEE KVYSGQVAC ARFLFNDRN LVTVGGTDA ALMLWELTE E
- >TC012483 RA
atgtttgat tgtgagctt gacgaagat acgccgttt gctgtcgtg accgtaccc aacatggct gctagttac gggaagaaa aacgggatt tggtacgcg gctcccgga ggacaaggc tggagtaag gcgacgagt cggaaggcc tcaattgca gagactgat gcaccgcca ccggggggt gggacgatc aagccaagt tccgggaga gtgattagg ataattaat aatatggac cacactgtt cagtgccgc gtcctttta aacttacgc acctcccag cccttcgag gaagtcctc gaagacctc ggccaagtt ttgaaaatg aacggtgcg aaacgcatg tacacagtg tcaggtcaa gaagtgcgc agtttttca caattaaga aacgaattc gctgatgtg gacactttt tatttgggg gttgggagt gtgggaggt ggggttggg ggtgtgatg ttatcacca gttcggagg gctcgctca cgggccccc cagaccgca atcgtggag gatatgagc aaacaaaga cgggcgagg agtaaaagc cgaccgcgg gctctctac gcccccgaa agcgaaatc gtgcgaacc aacgattac actttgtta gaagtactg aaggaggag ccggttagg gtcactatt aaagggttg aggaggacg ttttatcct cccatccat catgcaccg atggataac actccccct gagaagaaa atgcaattg gattatggg tatcgtggc accgactcg aggagaaat ctatgggtt ctcccaacg ggagaattg ctctatttt gttgcagct gtcgctgtt atgtatgac agagacgag gaggcccaa aggcactac acaggccac actgaggac attcaatgt atggactta catccctcg cgcgagatg gtggcgtct ggtcaaagg gcgggccgt aatcgcaaa acccaggcc catatccgc atctggagt acggaaacc ctccaaacg ctctacgtt ttcggaatg ggggaattc gaagttggg gtggcggca gttgcgttc tcacaattg aatggtggg agttatgtg ttggccgtt gatggagga cgggagagc attttgtcg gtgtggcag tggcaatgg ggacacctt ttggggaaa gtagccacc cttcaagaa gaactaacc ggagccgtt ttccaccct ctggacgac aatctcatg ataacacac ggcaaaggc cacctgacc ttttggaac agacgcaaa gacggcttt ttcgaacgt actgatatt atcaaacct ccttctcgt acacttatc actgcactg caatttgag caagacggc gatgtcatc actgcggac agtgacggt ttcatcacc atttacagt gttgatagt gacggtgct tactttgtt cgtttggag tacgaagcc cacaacaaa ggggtcagt tcgctggtg atgctgtcg gaaggaacg ctgctttcg ggtggtgac aaagatcgg aaaattgtc gcctgggat tccctccaa aactacaag aaaatcact gaaactaaa cttgctgag tctgctggt ggggtcagg tccatttat ccccaacgc cctggacgc aatgatggg aatatctac gtcgggact actaaaaat aatattttg gaagggtcg cttcaaagg aggttcaat caggttgtt ttcgggcat gggagacag ttgtgggga cttgcagtg catccagat gatgaagtt tttgccaca gcagggcat gataagaac attgccttg tggaggaag aataaattt atttggacg ttacaagtg gcttttgaa tgcatttct ctaagtttc cacccattc ggcgtcgcc ttagccgcc ggtagcacc gaaggccac ctcataatc atcaacact gaaaccgga acccttatc aacaccatc cgagtttgt ggctcaccc ctcagctgt gttggctac aaccctacc ggagacatg atcgcaata gcttcacaa aacggaagc atctattta ttcagagtg agccgagac ggattttcc tacaaaaaa tcgaacaag atcagaggc tcgcaacct ctcatccaa atggattgg agcacggat agcaatttc ttgcaaaca gtcactgcc gattacgat ttatgtttc tgggatgtc aaatctttg tcaccggaa aagagcccg attgctatg aaagacgtc aagtggtac actcagaac tcaacagtt ggctttttg gtcgccggt atttggaac aaccgtttc tatccaatg acttccatc atcagtacc gtgagccga tccgcggcc catgatttg ctaatttcg ggcgatact gacggttat atcagactt ttcagatac ccatgtttg agtcccaaa gccgaatac aacgaagaa aaagtttac agtggccaa gtggcctgt gctcgattc ctatttaac gatagaaat ttggtgaca gttggaggc actgacgct gccctcatg ctgtgggaa ttgacggaa gaatag
- >TC012483 RA
ttgcttgag ttttgtgac caatgtttg attgtgagc ttgacgaag atacgccgt ttgctgtcg tgaccgtac ccaacatgg ctgctagtt acgggaaga aaaacggga tttggtacg cggctcccg gaggacaag gctggagta aggcgacga gtcggaagg cctcaattg cagagactg atgcaccgc caccggggg gtgggacga tcaagccaa gttccggga gagtgatta ggataatta ataatatgg accacactg ttcagtgcc gcgtccttt taaacttac gcacctccc agcccttcg aggaagtcc tcgaagacc tcggccaag ttttgaaaa tgaacggtg cgaaacgca tgtacacag tgtcaggtc aagaagtgc gcagttttt cacaattaa gaaacgaat tcgctgatg tggacactt tttatttgg gggttggga gtgtgggag gtggggttg ggggtgtga tgttatcac cagttcgga gggctcgct cacgggccc cccagaccg caatcgtgg aggatatga gcaaacaaa gacgggcga ggagtaaaa gccgaccgc gggctctct acgcccccg aaagcgaaa tcgtgcgaa ccaacgatt acactttgt tagaagtac tgaaggagg agccggtta gggtcacta ttaaagggt tgaggagga cgttttatc ctcccatcc atcatgcac cgatggata acactcccc ctgagaaga aaatgcaat tggattatg ggtatcgtg gcaccgact cgaggagaa atctatggg ttctcccaa cgggagaat tgctctatt ttgttgcag ctgtcgctg ttatgtatg acagagacg aggaggccc aaaggcact acacaggcc acactgagg acattcaat gtatggact tacatccct cgcgcgaga tggtggcgt ctggtcaaa gggcgggcc gtaatcgca aaacccagg cccatatcc gcatctgga gtacggaaa ccctccaaa cgctctacg ttttcggaa tgggggaat tcgaagttg gggtggcgg cagttgcgt tctcacaat tgaatggtg ggagttatg tgttggccg ttgatggag gacgggaga gcattttgt cggtgtggc agtggcaat ggggacacc ttttgggga aagtagcca cccttcaag aagaactaa ccggagccg ttttccacc ctctggacg acaatctca tgataacac acggcaaag gccacctga ccttttgga acagacgca aagacggct ttttcgaac gtactgata ttatcaaac ctccttctc gtacactta tcactgcac tgcaatttg agcaagacg gcgatgtca tcactgcgg acagtgacg gtttcatca ccatttaca gtgttgata gtgacggtg cttactttg ttcgtttgg agtacgaag cccacaaca aaggggtca gttcgctgg tgatgctgt cggaaggaa cgctgcttt cgggtggtg acaaagatc ggaaaattg tcgcctggg attccctcc aaaactaca agaaaatca ctgaaacta aacttgctg agtctgctg gtggggtca ggtccattt atccccaac gccctggac gcaatgatg ggaatatct acgtcggga ctactaaaa ataatattt tggaagggt cgcttcaaa ggaggttca atcaggttg ttttcgggc atgggagac agttgtggg gacttgcag tgcatccag atgatgaag tttttgcca cagcagggc atgataaga acattgcct tgtggagga agaataaat ttatttgga cgttacaag tggcttttg aatgcattt ctctaagtt tccacccat tcggcgtcg ccttagccg ccggtagca ccgaaggcc acctcataa tcatcaaca ctgaaaccg gaaccctta tcaacacca tccgagttt gtggctcac ccctcagct gtgttggct acaacccta ccggagaca tgatcgcaa tagcttcac aaaacggaa gcatctatt tattcagag tgagccgag acggatttt cctacaaaa aatcgaaca agatcagag gctcgcaac ctctcatcc aaatggatt ggagcacgg atagcaatt tcttgcaaa cagtcactg ccgattacg atttatgtt tctgggatg tcaaatctt tgtcaccgg aaaagagcc cgattgcta tgaaagacg tcaagtggt acactcaga actcaacag ttggctttt tggtcgccg gtatttgga acaaccgtt tctatccaa tgacttcca tcatcagta ccgtgagcc gatccgcgg cccatgatt tgctaattt cgggcgata ctgacggtt atatcagac ttttcagat acccatgtt tgagtccca aagccgaat acaacgaag aaaaagttt acagtggcc aagtggcct gtgctcgat tcctattta acgatagaa atttggtga cagttggag gcactgacg ctgccctca tgctgtggg aattgacgg aagaatagc aaaacgcgt cttttttgt acttgcctt cgatagtta gacgttacg atgattatg aagattcag atttcgatt cgtctttcg aaaattctt tcgaagttc tcaacatta aatactgaa gtacggaga gcacacata tcctcacat agatctaaa tttagacca aataagttt taatcaggt gttattatg cttttagtt aaaaaaata tttatgaaa tatagtact gaaaaaccc agtgaatga gaaccaaag tgatacaat acctcaatt gcattgtta gaaaattgt cagaagctt taaaaatgg gcgatacga aacctttca ccttttgtt aaattagtt taattattt atatagtta taaatacac ctggaaact gccaatagc tatagaatt tattataat gaatactat aaatgtaga gaaacaaag caatgcatg gtagcttca aatatttac ctaaaaaac tttttaata ttaataaat ccattgtta tggt
