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Gene information
General information
TC012483 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 9113381..9130278
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC012483
    iBeetle screen iB_05248

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012483 RA
      MFDCELDED TPFAVVTVP NMAASYGKK NGIWYAAPG 
GQGWSKATS RKASIAETD APPPGGGTI KPSSGRVIR 
IINNMDHTV QCRVLLNLR TSQPFEEVL EDLGQVLKM 
NGAKRMYTV SGQEVRSFS QLRNEFADV DTFYLGVGS 
VGGGVGGVM LSPVRRARS RAPQTAIVE DMSKQRRAR 
SKSRPRALY APESEIVRT NDYTLLEVL KEEPVRVTI 
KGLRRTFYP PIHHAPMDN TPPEKKMQL DYGYRGTDS 
RRNLWVLPT GELLYFVAA VAVMYDRDE EAQRHYTGH 
TEDIQCMDL HPSREMVAS GQRAGRNRK TQAHIRIWS 
TETLQTLYV FGMGEFEVG VAAVAFSQL NGGSYVLAV 
DGGRESILS VWQWQWGHL LGKVATLQE ELTGAVFHP 
LDDNLMITH GKGHLTFWN RRKDGFFER TDIIKPPSR 
TLITALQFE QDGDVITAD SDGFITIYS VDSDGAYFV 
RLEYEAHNK GVSSLVMLS EGTLLSGGD KDRKIVAWD 
SLQNYKKIT ETKLAESAG GVRSIYPQR PGRNDGNIY 
VGTTKNNIL EGSLQRRFN QVVFGHGRQ LWGLAVHPD 
DEVFATAGH DKNIALWRK NKFIWTLQV AFECISLSF 
HPFGVALAA GSTEGHLII INTETGTLI NTIRVCGSP 
LSCVGYNPT GDMIAIASQ NGSIYLFRV SRDGFSYKK 
SNKIRGSQP LIQMDWSTD SNFLQTVTA DYDLCFWDV 
KSLSPEKSP IAMKDVKWY TQNSTVGFL VAGIWNNRF 
YPMTSIIST VSRSAAHDL LISGDTDGY IRLFRYPCL 
SPKAEYNEE KVYSGQVAC ARFLFNDRN LVTVGGTDA 
ALMLWELTE E
    CDS sequence
    • >TC012483 RA
      atgtttgat tgtgagctt gacgaagat acgccgttt 
gctgtcgtg accgtaccc aacatggct gctagttac 
gggaagaaa aacgggatt tggtacgcg gctcccgga 
ggacaaggc tggagtaag gcgacgagt cggaaggcc 
tcaattgca gagactgat gcaccgcca ccggggggt 
gggacgatc aagccaagt tccgggaga gtgattagg 
ataattaat aatatggac cacactgtt cagtgccgc 
gtcctttta aacttacgc acctcccag cccttcgag 
gaagtcctc gaagacctc ggccaagtt ttgaaaatg 
aacggtgcg aaacgcatg tacacagtg tcaggtcaa 
gaagtgcgc agtttttca caattaaga aacgaattc 
gctgatgtg gacactttt tatttgggg gttgggagt 
gtgggaggt ggggttggg ggtgtgatg ttatcacca 
gttcggagg gctcgctca cgggccccc cagaccgca 
atcgtggag gatatgagc aaacaaaga cgggcgagg 
agtaaaagc cgaccgcgg gctctctac gcccccgaa 
agcgaaatc gtgcgaacc aacgattac actttgtta 
gaagtactg aaggaggag ccggttagg gtcactatt 
aaagggttg aggaggacg ttttatcct cccatccat 
catgcaccg atggataac actccccct gagaagaaa 
atgcaattg gattatggg tatcgtggc accgactcg 
aggagaaat ctatgggtt ctcccaacg ggagaattg 
ctctatttt gttgcagct gtcgctgtt atgtatgac 
agagacgag gaggcccaa aggcactac acaggccac 
actgaggac attcaatgt atggactta catccctcg 
cgcgagatg gtggcgtct ggtcaaagg gcgggccgt 
aatcgcaaa acccaggcc catatccgc atctggagt 
acggaaacc ctccaaacg ctctacgtt ttcggaatg 
ggggaattc gaagttggg gtggcggca gttgcgttc 
tcacaattg aatggtggg agttatgtg ttggccgtt 
gatggagga cgggagagc attttgtcg gtgtggcag 
tggcaatgg ggacacctt ttggggaaa gtagccacc 
cttcaagaa gaactaacc ggagccgtt ttccaccct 
ctggacgac aatctcatg ataacacac ggcaaaggc 
cacctgacc ttttggaac agacgcaaa gacggcttt 
ttcgaacgt actgatatt atcaaacct ccttctcgt 
acacttatc actgcactg caatttgag caagacggc 
gatgtcatc actgcggac agtgacggt ttcatcacc 
atttacagt gttgatagt gacggtgct tactttgtt 
cgtttggag tacgaagcc cacaacaaa ggggtcagt 
tcgctggtg atgctgtcg gaaggaacg ctgctttcg 
ggtggtgac aaagatcgg aaaattgtc gcctgggat 
tccctccaa aactacaag aaaatcact gaaactaaa 
cttgctgag tctgctggt ggggtcagg tccatttat 
ccccaacgc cctggacgc aatgatggg aatatctac 
gtcgggact actaaaaat aatattttg gaagggtcg 
cttcaaagg aggttcaat caggttgtt ttcgggcat 
gggagacag ttgtgggga cttgcagtg catccagat 
gatgaagtt tttgccaca gcagggcat gataagaac 
attgccttg tggaggaag aataaattt atttggacg 
ttacaagtg gcttttgaa tgcatttct ctaagtttc 
cacccattc ggcgtcgcc ttagccgcc ggtagcacc 
gaaggccac ctcataatc atcaacact gaaaccgga 
acccttatc aacaccatc cgagtttgt ggctcaccc 
ctcagctgt gttggctac aaccctacc ggagacatg 
atcgcaata gcttcacaa aacggaagc atctattta 
ttcagagtg agccgagac ggattttcc tacaaaaaa 
tcgaacaag atcagaggc tcgcaacct ctcatccaa 
atggattgg agcacggat agcaatttc ttgcaaaca 
gtcactgcc gattacgat ttatgtttc tgggatgtc 
aaatctttg tcaccggaa aagagcccg attgctatg 
aaagacgtc aagtggtac actcagaac tcaacagtt 
ggctttttg gtcgccggt atttggaac aaccgtttc 
tatccaatg acttccatc atcagtacc gtgagccga 
tccgcggcc catgatttg ctaatttcg ggcgatact 
gacggttat atcagactt ttcagatac ccatgtttg 
agtcccaaa gccgaatac aacgaagaa aaagtttac 
agtggccaa gtggcctgt gctcgattc ctatttaac 
gatagaaat ttggtgaca gttggaggc actgacgct 
gccctcatg ctgtgggaa ttgacggaa gaatag
    mRNA sequence
    • >TC012483 RA
      ttgcttgag ttttgtgac caatgtttg attgtgagc 
ttgacgaag atacgccgt ttgctgtcg tgaccgtac 
ccaacatgg ctgctagtt acgggaaga aaaacggga 
tttggtacg cggctcccg gaggacaag gctggagta 
aggcgacga gtcggaagg cctcaattg cagagactg 
atgcaccgc caccggggg gtgggacga tcaagccaa 
gttccggga gagtgatta ggataatta ataatatgg 
accacactg ttcagtgcc gcgtccttt taaacttac 
gcacctccc agcccttcg aggaagtcc tcgaagacc 
tcggccaag ttttgaaaa tgaacggtg cgaaacgca 
tgtacacag tgtcaggtc aagaagtgc gcagttttt 
cacaattaa gaaacgaat tcgctgatg tggacactt 
tttatttgg gggttggga gtgtgggag gtggggttg 
ggggtgtga tgttatcac cagttcgga gggctcgct 
cacgggccc cccagaccg caatcgtgg aggatatga 
gcaaacaaa gacgggcga ggagtaaaa gccgaccgc 
gggctctct acgcccccg aaagcgaaa tcgtgcgaa 
ccaacgatt acactttgt tagaagtac tgaaggagg 
agccggtta gggtcacta ttaaagggt tgaggagga 
cgttttatc ctcccatcc atcatgcac cgatggata 
acactcccc ctgagaaga aaatgcaat tggattatg 
ggtatcgtg gcaccgact cgaggagaa atctatggg 
ttctcccaa cgggagaat tgctctatt ttgttgcag 
ctgtcgctg ttatgtatg acagagacg aggaggccc 
aaaggcact acacaggcc acactgagg acattcaat 
gtatggact tacatccct cgcgcgaga tggtggcgt 
ctggtcaaa gggcgggcc gtaatcgca aaacccagg 
cccatatcc gcatctgga gtacggaaa ccctccaaa 
cgctctacg ttttcggaa tgggggaat tcgaagttg 
gggtggcgg cagttgcgt tctcacaat tgaatggtg 
ggagttatg tgttggccg ttgatggag gacgggaga 
gcattttgt cggtgtggc agtggcaat ggggacacc 
ttttgggga aagtagcca cccttcaag aagaactaa 
ccggagccg ttttccacc ctctggacg acaatctca 
tgataacac acggcaaag gccacctga ccttttgga 
acagacgca aagacggct ttttcgaac gtactgata 
ttatcaaac ctccttctc gtacactta tcactgcac 
tgcaatttg agcaagacg gcgatgtca tcactgcgg 
acagtgacg gtttcatca ccatttaca gtgttgata 
gtgacggtg cttactttg ttcgtttgg agtacgaag 
cccacaaca aaggggtca gttcgctgg tgatgctgt 
cggaaggaa cgctgcttt cgggtggtg acaaagatc 
ggaaaattg tcgcctggg attccctcc aaaactaca 
agaaaatca ctgaaacta aacttgctg agtctgctg 
gtggggtca ggtccattt atccccaac gccctggac 
gcaatgatg ggaatatct acgtcggga ctactaaaa 
ataatattt tggaagggt cgcttcaaa ggaggttca 
atcaggttg ttttcgggc atgggagac agttgtggg 
gacttgcag tgcatccag atgatgaag tttttgcca 
cagcagggc atgataaga acattgcct tgtggagga 
agaataaat ttatttgga cgttacaag tggcttttg 
aatgcattt ctctaagtt tccacccat tcggcgtcg 
ccttagccg ccggtagca ccgaaggcc acctcataa 
tcatcaaca ctgaaaccg gaaccctta tcaacacca 
tccgagttt gtggctcac ccctcagct gtgttggct 
acaacccta ccggagaca tgatcgcaa tagcttcac 
aaaacggaa gcatctatt tattcagag tgagccgag 
acggatttt cctacaaaa aatcgaaca agatcagag 
gctcgcaac ctctcatcc aaatggatt ggagcacgg 
atagcaatt tcttgcaaa cagtcactg ccgattacg 
atttatgtt tctgggatg tcaaatctt tgtcaccgg 
aaaagagcc cgattgcta tgaaagacg tcaagtggt 
acactcaga actcaacag ttggctttt tggtcgccg 
gtatttgga acaaccgtt tctatccaa tgacttcca 
tcatcagta ccgtgagcc gatccgcgg cccatgatt 
tgctaattt cgggcgata ctgacggtt atatcagac 
ttttcagat acccatgtt tgagtccca aagccgaat 
acaacgaag aaaaagttt acagtggcc aagtggcct 
gtgctcgat tcctattta acgatagaa atttggtga 
cagttggag gcactgacg ctgccctca tgctgtggg 
aattgacgg aagaatagc aaaacgcgt cttttttgt 
acttgcctt cgatagtta gacgttacg atgattatg 
aagattcag atttcgatt cgtctttcg aaaattctt 
tcgaagttc tcaacatta aatactgaa gtacggaga 
gcacacata tcctcacat agatctaaa tttagacca 
aataagttt taatcaggt gttattatg cttttagtt 
aaaaaaata tttatgaaa tatagtact gaaaaaccc 
agtgaatga gaaccaaag tgatacaat acctcaatt 
gcattgtta gaaaattgt cagaagctt taaaaatgg 
gcgatacga aacctttca ccttttgtt aaattagtt 
taattattt atatagtta taaatacac ctggaaact 
gccaatagc tatagaatt tattataat gaatactat 
aaatgtaga gaaacaaag caatgcatg gtagcttca 
aatatttac ctaaaaaac tttttaata ttaataaat 
ccattgtta tggt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY