Status
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Gene information
General information
TC012483 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 9113381..9130278
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012483
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05248

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012483 RA
      MFDCELDED TPFAVVTVP NMAASYGKK NGIWYAAPG 
      GQGWSKATS RKASIAETD APPPGGGTI KPSSGRVIR 
      IINNMDHTV QCRVLLNLR TSQPFEEVL EDLGQVLKM 
      NGAKRMYTV SGQEVRSFS QLRNEFADV DTFYLGVGS 
      VGGGVGGVM LSPVRRARS RAPQTAIVE DMSKQRRAR 
      SKSRPRALY APESEIVRT NDYTLLEVL KEEPVRVTI 
      KGLRRTFYP PIHHAPMDN TPPEKKMQL DYGYRGTDS 
      RRNLWVLPT GELLYFVAA VAVMYDRDE EAQRHYTGH 
      TEDIQCMDL HPSREMVAS GQRAGRNRK TQAHIRIWS 
      TETLQTLYV FGMGEFEVG VAAVAFSQL NGGSYVLAV 
      DGGRESILS VWQWQWGHL LGKVATLQE ELTGAVFHP 
      LDDNLMITH GKGHLTFWN RRKDGFFER TDIIKPPSR 
      TLITALQFE QDGDVITAD SDGFITIYS VDSDGAYFV 
      RLEYEAHNK GVSSLVMLS EGTLLSGGD KDRKIVAWD 
      SLQNYKKIT ETKLAESAG GVRSIYPQR PGRNDGNIY 
      VGTTKNNIL EGSLQRRFN QVVFGHGRQ LWGLAVHPD 
      DEVFATAGH DKNIALWRK NKFIWTLQV AFECISLSF 
      HPFGVALAA GSTEGHLII INTETGTLI NTIRVCGSP 
      LSCVGYNPT GDMIAIASQ NGSIYLFRV SRDGFSYKK 
      SNKIRGSQP LIQMDWSTD SNFLQTVTA DYDLCFWDV 
      KSLSPEKSP IAMKDVKWY TQNSTVGFL VAGIWNNRF 
      YPMTSIIST VSRSAAHDL LISGDTDGY IRLFRYPCL 
      SPKAEYNEE KVYSGQVAC ARFLFNDRN LVTVGGTDA 
      ALMLWELTE E
    CDS sequence
    • >TC012483 RA
      atgtttgat tgtgagctt gacgaagat acgccgttt 
      gctgtcgtg accgtaccc aacatggct gctagttac 
      gggaagaaa aacgggatt tggtacgcg gctcccgga 
      ggacaaggc tggagtaag gcgacgagt cggaaggcc 
      tcaattgca gagactgat gcaccgcca ccggggggt 
      gggacgatc aagccaagt tccgggaga gtgattagg 
      ataattaat aatatggac cacactgtt cagtgccgc 
      gtcctttta aacttacgc acctcccag cccttcgag 
      gaagtcctc gaagacctc ggccaagtt ttgaaaatg 
      aacggtgcg aaacgcatg tacacagtg tcaggtcaa 
      gaagtgcgc agtttttca caattaaga aacgaattc 
      gctgatgtg gacactttt tatttgggg gttgggagt 
      gtgggaggt ggggttggg ggtgtgatg ttatcacca 
      gttcggagg gctcgctca cgggccccc cagaccgca 
      atcgtggag gatatgagc aaacaaaga cgggcgagg 
      agtaaaagc cgaccgcgg gctctctac gcccccgaa 
      agcgaaatc gtgcgaacc aacgattac actttgtta 
      gaagtactg aaggaggag ccggttagg gtcactatt 
      aaagggttg aggaggacg ttttatcct cccatccat 
      catgcaccg atggataac actccccct gagaagaaa 
      atgcaattg gattatggg tatcgtggc accgactcg 
      aggagaaat ctatgggtt ctcccaacg ggagaattg 
      ctctatttt gttgcagct gtcgctgtt atgtatgac 
      agagacgag gaggcccaa aggcactac acaggccac 
      actgaggac attcaatgt atggactta catccctcg 
      cgcgagatg gtggcgtct ggtcaaagg gcgggccgt 
      aatcgcaaa acccaggcc catatccgc atctggagt 
      acggaaacc ctccaaacg ctctacgtt ttcggaatg 
      ggggaattc gaagttggg gtggcggca gttgcgttc 
      tcacaattg aatggtggg agttatgtg ttggccgtt 
      gatggagga cgggagagc attttgtcg gtgtggcag 
      tggcaatgg ggacacctt ttggggaaa gtagccacc 
      cttcaagaa gaactaacc ggagccgtt ttccaccct 
      ctggacgac aatctcatg ataacacac ggcaaaggc 
      cacctgacc ttttggaac agacgcaaa gacggcttt 
      ttcgaacgt actgatatt atcaaacct ccttctcgt 
      acacttatc actgcactg caatttgag caagacggc 
      gatgtcatc actgcggac agtgacggt ttcatcacc 
      atttacagt gttgatagt gacggtgct tactttgtt 
      cgtttggag tacgaagcc cacaacaaa ggggtcagt 
      tcgctggtg atgctgtcg gaaggaacg ctgctttcg 
      ggtggtgac aaagatcgg aaaattgtc gcctgggat 
      tccctccaa aactacaag aaaatcact gaaactaaa 
      cttgctgag tctgctggt ggggtcagg tccatttat 
      ccccaacgc cctggacgc aatgatggg aatatctac 
      gtcgggact actaaaaat aatattttg gaagggtcg 
      cttcaaagg aggttcaat caggttgtt ttcgggcat 
      gggagacag ttgtgggga cttgcagtg catccagat 
      gatgaagtt tttgccaca gcagggcat gataagaac 
      attgccttg tggaggaag aataaattt atttggacg 
      ttacaagtg gcttttgaa tgcatttct ctaagtttc 
      cacccattc ggcgtcgcc ttagccgcc ggtagcacc 
      gaaggccac ctcataatc atcaacact gaaaccgga 
      acccttatc aacaccatc cgagtttgt ggctcaccc 
      ctcagctgt gttggctac aaccctacc ggagacatg 
      atcgcaata gcttcacaa aacggaagc atctattta 
      ttcagagtg agccgagac ggattttcc tacaaaaaa 
      tcgaacaag atcagaggc tcgcaacct ctcatccaa 
      atggattgg agcacggat agcaatttc ttgcaaaca 
      gtcactgcc gattacgat ttatgtttc tgggatgtc 
      aaatctttg tcaccggaa aagagcccg attgctatg 
      aaagacgtc aagtggtac actcagaac tcaacagtt 
      ggctttttg gtcgccggt atttggaac aaccgtttc 
      tatccaatg acttccatc atcagtacc gtgagccga 
      tccgcggcc catgatttg ctaatttcg ggcgatact 
      gacggttat atcagactt ttcagatac ccatgtttg 
      agtcccaaa gccgaatac aacgaagaa aaagtttac 
      agtggccaa gtggcctgt gctcgattc ctatttaac 
      gatagaaat ttggtgaca gttggaggc actgacgct 
      gccctcatg ctgtgggaa ttgacggaa gaatag
    mRNA sequence
    • >TC012483 RA
      ttgcttgag ttttgtgac caatgtttg attgtgagc 
      ttgacgaag atacgccgt ttgctgtcg tgaccgtac 
      ccaacatgg ctgctagtt acgggaaga aaaacggga 
      tttggtacg cggctcccg gaggacaag gctggagta 
      aggcgacga gtcggaagg cctcaattg cagagactg 
      atgcaccgc caccggggg gtgggacga tcaagccaa 
      gttccggga gagtgatta ggataatta ataatatgg 
      accacactg ttcagtgcc gcgtccttt taaacttac 
      gcacctccc agcccttcg aggaagtcc tcgaagacc 
      tcggccaag ttttgaaaa tgaacggtg cgaaacgca 
      tgtacacag tgtcaggtc aagaagtgc gcagttttt 
      cacaattaa gaaacgaat tcgctgatg tggacactt 
      tttatttgg gggttggga gtgtgggag gtggggttg 
      ggggtgtga tgttatcac cagttcgga gggctcgct 
      cacgggccc cccagaccg caatcgtgg aggatatga 
      gcaaacaaa gacgggcga ggagtaaaa gccgaccgc 
      gggctctct acgcccccg aaagcgaaa tcgtgcgaa 
      ccaacgatt acactttgt tagaagtac tgaaggagg 
      agccggtta gggtcacta ttaaagggt tgaggagga 
      cgttttatc ctcccatcc atcatgcac cgatggata 
      acactcccc ctgagaaga aaatgcaat tggattatg 
      ggtatcgtg gcaccgact cgaggagaa atctatggg 
      ttctcccaa cgggagaat tgctctatt ttgttgcag 
      ctgtcgctg ttatgtatg acagagacg aggaggccc 
      aaaggcact acacaggcc acactgagg acattcaat 
      gtatggact tacatccct cgcgcgaga tggtggcgt 
      ctggtcaaa gggcgggcc gtaatcgca aaacccagg 
      cccatatcc gcatctgga gtacggaaa ccctccaaa 
      cgctctacg ttttcggaa tgggggaat tcgaagttg 
      gggtggcgg cagttgcgt tctcacaat tgaatggtg 
      ggagttatg tgttggccg ttgatggag gacgggaga 
      gcattttgt cggtgtggc agtggcaat ggggacacc 
      ttttgggga aagtagcca cccttcaag aagaactaa 
      ccggagccg ttttccacc ctctggacg acaatctca 
      tgataacac acggcaaag gccacctga ccttttgga 
      acagacgca aagacggct ttttcgaac gtactgata 
      ttatcaaac ctccttctc gtacactta tcactgcac 
      tgcaatttg agcaagacg gcgatgtca tcactgcgg 
      acagtgacg gtttcatca ccatttaca gtgttgata 
      gtgacggtg cttactttg ttcgtttgg agtacgaag 
      cccacaaca aaggggtca gttcgctgg tgatgctgt 
      cggaaggaa cgctgcttt cgggtggtg acaaagatc 
      ggaaaattg tcgcctggg attccctcc aaaactaca 
      agaaaatca ctgaaacta aacttgctg agtctgctg 
      gtggggtca ggtccattt atccccaac gccctggac 
      gcaatgatg ggaatatct acgtcggga ctactaaaa 
      ataatattt tggaagggt cgcttcaaa ggaggttca 
      atcaggttg ttttcgggc atgggagac agttgtggg 
      gacttgcag tgcatccag atgatgaag tttttgcca 
      cagcagggc atgataaga acattgcct tgtggagga 
      agaataaat ttatttgga cgttacaag tggcttttg 
      aatgcattt ctctaagtt tccacccat tcggcgtcg 
      ccttagccg ccggtagca ccgaaggcc acctcataa 
      tcatcaaca ctgaaaccg gaaccctta tcaacacca 
      tccgagttt gtggctcac ccctcagct gtgttggct 
      acaacccta ccggagaca tgatcgcaa tagcttcac 
      aaaacggaa gcatctatt tattcagag tgagccgag 
      acggatttt cctacaaaa aatcgaaca agatcagag 
      gctcgcaac ctctcatcc aaatggatt ggagcacgg 
      atagcaatt tcttgcaaa cagtcactg ccgattacg 
      atttatgtt tctgggatg tcaaatctt tgtcaccgg 
      aaaagagcc cgattgcta tgaaagacg tcaagtggt 
      acactcaga actcaacag ttggctttt tggtcgccg 
      gtatttgga acaaccgtt tctatccaa tgacttcca 
      tcatcagta ccgtgagcc gatccgcgg cccatgatt 
      tgctaattt cgggcgata ctgacggtt atatcagac 
      ttttcagat acccatgtt tgagtccca aagccgaat 
      acaacgaag aaaaagttt acagtggcc aagtggcct 
      gtgctcgat tcctattta acgatagaa atttggtga 
      cagttggag gcactgacg ctgccctca tgctgtggg 
      aattgacgg aagaatagc aaaacgcgt cttttttgt 
      acttgcctt cgatagtta gacgttacg atgattatg 
      aagattcag atttcgatt cgtctttcg aaaattctt 
      tcgaagttc tcaacatta aatactgaa gtacggaga 
      gcacacata tcctcacat agatctaaa tttagacca 
      aataagttt taatcaggt gttattatg cttttagtt 
      aaaaaaata tttatgaaa tatagtact gaaaaaccc 
      agtgaatga gaaccaaag tgatacaat acctcaatt 
      gcattgtta gaaaattgt cagaagctt taaaaatgg 
      gcgatacga aacctttca ccttttgtt aaattagtt 
      taattattt atatagtta taaatacac ctggaaact 
      gccaatagc tatagaatt tattataat gaatactat 
      aaatgtaga gaaacaaag caatgcatg gtagcttca 
      aatatttac ctaaaaaac tttttaata ttaataaat 
      ccattgtta tggt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY