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Gene information
General information
TC012457 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 7778976..7800485
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012457
    iBeetle screen iB_05245

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature partiallynot present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
    appendages random remaining - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva dorsal not closed - Penetrance (percentage of animals/eggs on slide): less than 30%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012457 RA
      MDNEQPHQE LVKCVVVGD TAVGKTRLI CARACNKQV 
      SLAQLLATH VPTVWAIDQ YRIYKDVLE RSWEVVDSV 
      NVSLRLWDT FGDHEKDRR FAYGRSDVV LLCFSINNP 
      VSLRNCRLM WFPEIRRFC PSTPVLLVG CKNDLRYMY 
      RDEAYLSYF RDRSPFVRA TRKSDLVMP DEARDVARE 
      LGLFYYETS VLTYYGVNE VFENAIRAA LIARRQQRF 
      WMTNLKKVQ RPLLQAPFK PPPPMKPDV HVVVGSYGE 
      HMHQLYLGR AHTDIVLVV GSVGFPAHR FVLAACSRA 
      FYRLLAADL IARSTSDSS MVSSLGDFA DETECLVRA 
      EQKTCKRRA SCQALPSAR ELDHPAFHC IRSVQPEGQ 
      TVVTLSKLV TPGAMRQCL QFAYTGTID RSALNLQET 
      REAAEFLEL PQLLVLLTK HPFVGPDHP DETYENFLK 
      RRLQDICLD QGLFADVIF ELDDGACAA HKAMLTARC 
      DVMKAMFSG DFRESQAKV IEFPGVREY TFHKLLCYL 
      YTDEIPAVS AVRCVNLLE LANRLCLPR LVNLVEKRV 
      IEDLECLQP SEAIEHCLR LLEPVKLHN GHQLADWCM 
      NHLCVNYNK LCRMSPRSL RLLHPDNQA YLVEHRWPP 
      VWYLKDYDY YQKCLAERD KELKPTIKT SSGCLCFSR 
      KSESDPALS SSQL
    CDS sequence
    • >TC012457 RA
      atggataat gaacaaccc caccaggaa ctcgtcaag 
      tgcgtggtg gttggcgac acggccgtc ggcaagacc 
      cgtctgatc tgcgcccgt gcatgcaac aagcaagtc 
      tctttggcc cagctgctc gcaacccac gtccccaca 
      gtatgggcg atcgaccag taccgcatc tacaaggac 
      gtgctcgag cgctcctgg gaagtggtc gacagcgtc 
      aacgtctct ctacgtctc tgggacacg ttcggcgac 
      cacgagaag gatcgccgc ttcgcctac ggccgctcc 
      gacgtcgtc ctcctctgc ttcagcatc aacaacccc 
      gtctcgctg cgcaactgc cgcctcatg tggttcccc 
      gagatccgg cgcttctgt ccgagcacg ccggtgctg 
      ctcgtcggg tgcaagaac gacctccgg tacatgtac 
      cgagacgag gcctatctc agctacttc cgcgaccgc 
      agccccttt gtgagggcc acgcgcaag agcgacctc 
      gtcatgccg gacgaggcg agggacgtc gccagggaa 
      ttggggctc ttttactac gaaacgtcg gtacttacc 
      tactacgga gttaacgaa gtgttcgaa aacgccatc 
      cgcgcggcc ctcatcgcg cgccgccag cagcgcttc 
      tggatgacg aacctgaag aaggtccag cggccgttg 
      ctgcaggcg ccgttcaag ccgcccccg ccgatgaag 
      cccgacgtg cacgtggtg gtcggctcc tacggcgag 
      cacatgcac cagctgtac ctgggtcgc gcgcacacc 
      gacatcgtg ctcgtcgtg ggctcggtg ggcttcccg 
      gcgcaccgc ttcgtgctc gccgcctgc agccgcgcc 
      ttctaccgg ctgctggcc gccgacctc atcgcgcgc 
      agcaccagc gactccagc atggtgagc tcgctgggc 
      gacttcgcc gacgagacc gagtgcctg gtgcgcgcc 
      gagcagaag acgtgcaag cggcgcgcc agctgccag 
      gcgctgccg tcggcgcgc gagctcgac caccccgcc 
      ttccactgc atcaggagc gtgcagccc gagggccag 
      acggtggtc acgctcagc aagctggtg acgcccggc 
      gccatgcgg cagtgcctg cagttcgcc tacaccggc 
      accatcgac cgcagcgcc ctcaacctg caggagacg 
      cgcgaggcg gccgagttc ctcgagctg ccccagctg 
      ctggtgctg ctgaccaag cacccgttc gtggggccc 
      gaccatccg gacgagacc tacgagaac tttctcaag 
      aggcgcctc caggatatc tgtctggat cagggactt 
      ttcgccgat gttatcttc gagctggac gatggggcg 
      tgtgcggcg cataaagcg atgcttacg gcgaggtgt 
      gacgtcatg aaagctatg tttagcggg gatttccgg 
      gaaagtcag gccaaagtc atcgagttc cccggagtc 
      cgcgagtac accttccac aaattactg tgttactta 
      tacaccgac gaaatcccc gcagtgtcg gcggtccgc 
      tgcgtcaat ttactcgag ttagcaaat cgtctttgc 
      ttgcctcgt ctcgtcaat ttagtcgaa aagcgagtc 
      atcgaggac ttggagtgc ttgcagccg tctgaggcc 
      atcgagcac tgtctgcga ctgctagaa cccgtcaag 
      ctgcacaac gggcaccag ctggccgac tggtgcatg 
      aaccacctc tgcgtcaac tacaacaag ctgtgccgc 
      atgtcgccc cgcagcctc cgcctgctg caccccgac 
      aaccaggcg tacctcgtg gagcaccgc tggcccccc 
      gtttggtac ttgaaggac tacgactat tatcaaaag 
      tgtttggcc gaacgcgac aaagaactg aagcccaca 
      atcaaaaca agctcgggc tgtttatgc ttctctcgg 
      aaatcagag tctgacccg gccctgtcc tcgagccag 
      ctctga
    mRNA sequence
    • >TC012457 RA
      tcgcgcttg acgtcacgg cgcccaaaa atcaatcgc 
      cgttatttg cagcgcgaa aaatcggag gcatgctga 
      ctttttccg aaaaaatcg ccgctttgc gcgggcctt 
      tattcgata atgggcgcg tttagcggt cgctggtcg 
      caaattagc gtcgatttg atcaagtcg tgccgtcac 
      cgcgacgtc agagggcca gttcgcccg agtccgccg 
      aggagtgca gtgcagtgc aggccggac gtccaataa 
      atgctcttc gacggttct tcgccaaac tgatgaatg 
      gggccgtgg tgtgatggc catcgtcgc cgcagcgtt 
      cgccgggga tggataatg aacaacccc accaggaac 
      tcgtcaagt gcgtggtgg ttggcgaca cggccgtcg 
      gcaagaccc gtctgatct gcgcccgtg catgcaaca 
      agcaagtct ctttggccc agctgctcg caacccacg 
      tccccacag tatgggcga tcgaccagt accgcatct 
      acaaggacg tgctcgagc gctcctggg aagtggtcg 
      acagcgtca acgtctctc tacgtctct gggacacgt 
      tcggcgacc acgagaagg atcgccgct tcgcctacg 
      gccgctccg acgtcgtcc tcctctgct tcagcatca 
      acaaccccg tctcgctgc gcaactgcc gcctcatgt 
      ggttccccg agatccggc gcttctgtc cgagcacgc 
      cggtgctgc tcgtcgggt gcaagaacg acctccggt 
      acatgtacc gagacgagg cctatctca gctacttcc 
      gcgaccgca gcccctttg tgagggcca cgcgcaaga 
      gcgacctcg tcatgccgg acgaggcga gggacgtcg 
      ccagggaat tggggctct tttactacg aaacgtcgg 
      tacttacct actacggag ttaacgaag tgttcgaaa 
      acgccatcc gcgcggccc tcatcgcgc gccgccagc 
      agcgcttct ggatgacga acctgaaga aggtccagc 
      ggccgttgc tgcaggcgc cgttcaagc cgcccccgc 
      cgatgaagc ccgacgtgc acgtggtgg tcggctcct 
      acggcgagc acatgcacc agctgtacc tgggtcgcg 
      cgcacaccg acatcgtgc tcgtcgtgg gctcggtgg 
      gcttcccgg cgcaccgct tcgtgctcg ccgcctgca 
      gccgcgcct tctaccggc tgctggccg ccgacctca 
      tcgcgcgca gcaccagcg actccagca tggtgagct 
      cgctgggcg acttcgccg acgagaccg agtgcctgg 
      tgcgcgccg agcagaaga cgtgcaagc ggcgcgcca 
      gctgccagg cgctgccgt cggcgcgcg agctcgacc 
      accccgcct tccactgca tcaggagcg tgcagcccg 
      agggccaga cggtggtca cgctcagca agctggtga 
      cgcccggcg ccatgcggc agtgcctgc agttcgcct 
      acaccggca ccatcgacc gcagcgccc tcaacctgc 
      aggagacgc gcgaggcgg ccgagttcc tcgagctgc 
      cccagctgc tggtgctgc tgaccaagc acccgttcg 
      tggggcccg accatccgg acgagacct acgagaact 
      ttctcaaga ggcgcctcc aggatatct gtctggatc 
      agggacttt tcgccgatg ttatcttcg agctggacg 
      atggggcgt gtgcggcgc ataaagcga tgcttacgg 
      cgaggtgtg acgtcatga aagctatgt ttagcgggg 
      atttccggg aaagtcagg ccaaagtca tcgagttcc 
      ccggagtcc gcgagtaca ccttccaca aattactgt 
      gttacttat acaccgacg aaatccccg cagtgtcgg 
      cggtccgct gcgtcaatt tactcgagt tagcaaatc 
      gtctttgct tgcctcgtc tcgtcaatt tagtcgaaa 
      agcgagtca tcgaggact tggagtgct tgcagccgt 
      ctgaggcca tcgagcact gtctgcgac tgctagaac 
      ccgtcaagc tgcacaacg ggcaccagc tggccgact 
      ggtgcatga accacctct gcgtcaact acaacaagc 
      tgtgccgca tgtcgcccc gcagcctcc gcctgctgc 
      accccgaca accaggcgt acctcgtgg agcaccgct 
      ggccccccg tttggtact tgaaggact acgactatt 
      atcaaaagt gtttggccg aacgcgaca aagaactga 
      agcccacaa tcaaaacaa gctcgggct gtttatgct 
      tctctcgga aatcagagt ctgacccgg ccctgtcct 
      cgagccagc tctgaccgg gactactgt gactgttca 
      aatgcattt aactcattt cacctttca cgattctct 
      cataattgt cttgacata ttcgtaggt taagtgcag 
      gcggccacg atgtacaga gtatcgacc acatcgccg 
      aggtacagc taacctatc tgaagatgt cgtctccgt 
      caaacgaac ccctgctct tactgtcgt gcctttgag 
      cgcggccat tgccagcat tattaaagt tcgagggct 
      gtcgttatg caacgagcg tatgcgagt gcataccta 
      gacacccga gaaatttaa gcgttttcg catgagtgt 
      tattcaatt tttttgatg gaatatttt aacttaaaa 
      cacgttgct atggggagc gtgttttaa aatagtgaa 
      aacacgttg ctatgggaa acgtgtttt aaaatagtg 
      tataatcta ggaaaaaac attattgca tcgtatatt 
      tgaaaattc tttaggaac atcctcggt taaatttga 
      cgagtcttg caatcacag tttgtaaat ttaattata 
      attgttata gtaattttt ttatacaaa ttttcattt 
      gcagcggtt catttaaaa tatcacagt taatgaacc 
      cttcacaat aactgaata attctttct gcacttgat 
      agccaaagg ttcgaaggg tcttttcaa aaagtaaac 
      tttggaaaa atacgataa aattgtaat tagtggatt 
      cgcacattg tttcagagg atacactgt tgatggcaa 
      aaaaacgaa cactgtaac gaaaaaata taaaaatta 
      cactgaaat ctctcttaa aggacacct cctatgagc 
      ggacgcctc tgataaacg gacattttt gttggctct 
      gttttgagc taatgtatt ctaaatagt attgtttct 
      cttttaagc ggactctcc aataaactg agaatcaca 
      taagccggt acatgtttc atttcagtg ttaaactaa 
      gtcagattc agacctcag aagataaaa ttatcttat 
      gggtattat ttattgcac tagttcttc tatgcagcg 
      ttttaatgg catataaaa ctaaaaaag cgtatgcta 
      acactaaaa gaaaaaaaa aacaaaatt ttagcaatg 
      aaatttgaa atacctaag cagcaaata ttgtcaaaa 
      atgaagatg aactcgtgg tagaaacgt ttgtgaaaa 
      gcaaagttt tttttaaaa attaaagga ctgaaaata 
      ttgataaat taacttatg gttggttta tcaaatcat 
      taaaaaata ttccacttt cttgatcaa ttgttggga 
      tgaaaacta aagagttgg cgacgaaat tggaaaatc 
      aaattttta aggcaccat taaggtgac aagaaaagt 
      tttaaaatc acgatacaa gttttaagt acatatctg 
      atgaacatg atatagaga agtcagaag ttaaatatt 
      ttttttatg aaatagttt ataacaatt gttgtacca 
      atacttata atcatgctg ctacctatt taataaatt 
      ctttttagt ttg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY