Status
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Gene information
General information
TC012457 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 7778976..7800485
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC012457
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05245

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature partiallynot present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
    appendages random remaining - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva dorsal not closed - Penetrance (percentage of animals/eggs on slide): less than 30%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012457 RA
      MDNEQPHQE LVKCVVVGD TAVGKTRLI CARACNKQV 
SLAQLLATH VPTVWAIDQ YRIYKDVLE RSWEVVDSV 
NVSLRLWDT FGDHEKDRR FAYGRSDVV LLCFSINNP 
VSLRNCRLM WFPEIRRFC PSTPVLLVG CKNDLRYMY 
RDEAYLSYF RDRSPFVRA TRKSDLVMP DEARDVARE 
LGLFYYETS VLTYYGVNE VFENAIRAA LIARRQQRF 
WMTNLKKVQ RPLLQAPFK PPPPMKPDV HVVVGSYGE 
HMHQLYLGR AHTDIVLVV GSVGFPAHR FVLAACSRA 
FYRLLAADL IARSTSDSS MVSSLGDFA DETECLVRA 
EQKTCKRRA SCQALPSAR ELDHPAFHC IRSVQPEGQ 
TVVTLSKLV TPGAMRQCL QFAYTGTID RSALNLQET 
REAAEFLEL PQLLVLLTK HPFVGPDHP DETYENFLK 
RRLQDICLD QGLFADVIF ELDDGACAA HKAMLTARC 
DVMKAMFSG DFRESQAKV IEFPGVREY TFHKLLCYL 
YTDEIPAVS AVRCVNLLE LANRLCLPR LVNLVEKRV 
IEDLECLQP SEAIEHCLR LLEPVKLHN GHQLADWCM 
NHLCVNYNK LCRMSPRSL RLLHPDNQA YLVEHRWPP 
VWYLKDYDY YQKCLAERD KELKPTIKT SSGCLCFSR 
KSESDPALS SSQL
    CDS sequence
    • >TC012457 RA
      atggataat gaacaaccc caccaggaa ctcgtcaag 
tgcgtggtg gttggcgac acggccgtc ggcaagacc 
cgtctgatc tgcgcccgt gcatgcaac aagcaagtc 
tctttggcc cagctgctc gcaacccac gtccccaca 
gtatgggcg atcgaccag taccgcatc tacaaggac 
gtgctcgag cgctcctgg gaagtggtc gacagcgtc 
aacgtctct ctacgtctc tgggacacg ttcggcgac 
cacgagaag gatcgccgc ttcgcctac ggccgctcc 
gacgtcgtc ctcctctgc ttcagcatc aacaacccc 
gtctcgctg cgcaactgc cgcctcatg tggttcccc 
gagatccgg cgcttctgt ccgagcacg ccggtgctg 
ctcgtcggg tgcaagaac gacctccgg tacatgtac 
cgagacgag gcctatctc agctacttc cgcgaccgc 
agccccttt gtgagggcc acgcgcaag agcgacctc 
gtcatgccg gacgaggcg agggacgtc gccagggaa 
ttggggctc ttttactac gaaacgtcg gtacttacc 
tactacgga gttaacgaa gtgttcgaa aacgccatc 
cgcgcggcc ctcatcgcg cgccgccag cagcgcttc 
tggatgacg aacctgaag aaggtccag cggccgttg 
ctgcaggcg ccgttcaag ccgcccccg ccgatgaag 
cccgacgtg cacgtggtg gtcggctcc tacggcgag 
cacatgcac cagctgtac ctgggtcgc gcgcacacc 
gacatcgtg ctcgtcgtg ggctcggtg ggcttcccg 
gcgcaccgc ttcgtgctc gccgcctgc agccgcgcc 
ttctaccgg ctgctggcc gccgacctc atcgcgcgc 
agcaccagc gactccagc atggtgagc tcgctgggc 
gacttcgcc gacgagacc gagtgcctg gtgcgcgcc 
gagcagaag acgtgcaag cggcgcgcc agctgccag 
gcgctgccg tcggcgcgc gagctcgac caccccgcc 
ttccactgc atcaggagc gtgcagccc gagggccag 
acggtggtc acgctcagc aagctggtg acgcccggc 
gccatgcgg cagtgcctg cagttcgcc tacaccggc 
accatcgac cgcagcgcc ctcaacctg caggagacg 
cgcgaggcg gccgagttc ctcgagctg ccccagctg 
ctggtgctg ctgaccaag cacccgttc gtggggccc 
gaccatccg gacgagacc tacgagaac tttctcaag 
aggcgcctc caggatatc tgtctggat cagggactt 
ttcgccgat gttatcttc gagctggac gatggggcg 
tgtgcggcg cataaagcg atgcttacg gcgaggtgt 
gacgtcatg aaagctatg tttagcggg gatttccgg 
gaaagtcag gccaaagtc atcgagttc cccggagtc 
cgcgagtac accttccac aaattactg tgttactta 
tacaccgac gaaatcccc gcagtgtcg gcggtccgc 
tgcgtcaat ttactcgag ttagcaaat cgtctttgc 
ttgcctcgt ctcgtcaat ttagtcgaa aagcgagtc 
atcgaggac ttggagtgc ttgcagccg tctgaggcc 
atcgagcac tgtctgcga ctgctagaa cccgtcaag 
ctgcacaac gggcaccag ctggccgac tggtgcatg 
aaccacctc tgcgtcaac tacaacaag ctgtgccgc 
atgtcgccc cgcagcctc cgcctgctg caccccgac 
aaccaggcg tacctcgtg gagcaccgc tggcccccc 
gtttggtac ttgaaggac tacgactat tatcaaaag 
tgtttggcc gaacgcgac aaagaactg aagcccaca 
atcaaaaca agctcgggc tgtttatgc ttctctcgg 
aaatcagag tctgacccg gccctgtcc tcgagccag 
ctctga
    mRNA sequence
    • >TC012457 RA
      tcgcgcttg acgtcacgg cgcccaaaa atcaatcgc 
cgttatttg cagcgcgaa aaatcggag gcatgctga 
ctttttccg aaaaaatcg ccgctttgc gcgggcctt 
tattcgata atgggcgcg tttagcggt cgctggtcg 
caaattagc gtcgatttg atcaagtcg tgccgtcac 
cgcgacgtc agagggcca gttcgcccg agtccgccg 
aggagtgca gtgcagtgc aggccggac gtccaataa 
atgctcttc gacggttct tcgccaaac tgatgaatg 
gggccgtgg tgtgatggc catcgtcgc cgcagcgtt 
cgccgggga tggataatg aacaacccc accaggaac 
tcgtcaagt gcgtggtgg ttggcgaca cggccgtcg 
gcaagaccc gtctgatct gcgcccgtg catgcaaca 
agcaagtct ctttggccc agctgctcg caacccacg 
tccccacag tatgggcga tcgaccagt accgcatct 
acaaggacg tgctcgagc gctcctggg aagtggtcg 
acagcgtca acgtctctc tacgtctct gggacacgt 
tcggcgacc acgagaagg atcgccgct tcgcctacg 
gccgctccg acgtcgtcc tcctctgct tcagcatca 
acaaccccg tctcgctgc gcaactgcc gcctcatgt 
ggttccccg agatccggc gcttctgtc cgagcacgc 
cggtgctgc tcgtcgggt gcaagaacg acctccggt 
acatgtacc gagacgagg cctatctca gctacttcc 
gcgaccgca gcccctttg tgagggcca cgcgcaaga 
gcgacctcg tcatgccgg acgaggcga gggacgtcg 
ccagggaat tggggctct tttactacg aaacgtcgg 
tacttacct actacggag ttaacgaag tgttcgaaa 
acgccatcc gcgcggccc tcatcgcgc gccgccagc 
agcgcttct ggatgacga acctgaaga aggtccagc 
ggccgttgc tgcaggcgc cgttcaagc cgcccccgc 
cgatgaagc ccgacgtgc acgtggtgg tcggctcct 
acggcgagc acatgcacc agctgtacc tgggtcgcg 
cgcacaccg acatcgtgc tcgtcgtgg gctcggtgg 
gcttcccgg cgcaccgct tcgtgctcg ccgcctgca 
gccgcgcct tctaccggc tgctggccg ccgacctca 
tcgcgcgca gcaccagcg actccagca tggtgagct 
cgctgggcg acttcgccg acgagaccg agtgcctgg 
tgcgcgccg agcagaaga cgtgcaagc ggcgcgcca 
gctgccagg cgctgccgt cggcgcgcg agctcgacc 
accccgcct tccactgca tcaggagcg tgcagcccg 
agggccaga cggtggtca cgctcagca agctggtga 
cgcccggcg ccatgcggc agtgcctgc agttcgcct 
acaccggca ccatcgacc gcagcgccc tcaacctgc 
aggagacgc gcgaggcgg ccgagttcc tcgagctgc 
cccagctgc tggtgctgc tgaccaagc acccgttcg 
tggggcccg accatccgg acgagacct acgagaact 
ttctcaaga ggcgcctcc aggatatct gtctggatc 
agggacttt tcgccgatg ttatcttcg agctggacg 
atggggcgt gtgcggcgc ataaagcga tgcttacgg 
cgaggtgtg acgtcatga aagctatgt ttagcgggg 
atttccggg aaagtcagg ccaaagtca tcgagttcc 
ccggagtcc gcgagtaca ccttccaca aattactgt 
gttacttat acaccgacg aaatccccg cagtgtcgg 
cggtccgct gcgtcaatt tactcgagt tagcaaatc 
gtctttgct tgcctcgtc tcgtcaatt tagtcgaaa 
agcgagtca tcgaggact tggagtgct tgcagccgt 
ctgaggcca tcgagcact gtctgcgac tgctagaac 
ccgtcaagc tgcacaacg ggcaccagc tggccgact 
ggtgcatga accacctct gcgtcaact acaacaagc 
tgtgccgca tgtcgcccc gcagcctcc gcctgctgc 
accccgaca accaggcgt acctcgtgg agcaccgct 
ggccccccg tttggtact tgaaggact acgactatt 
atcaaaagt gtttggccg aacgcgaca aagaactga 
agcccacaa tcaaaacaa gctcgggct gtttatgct 
tctctcgga aatcagagt ctgacccgg ccctgtcct 
cgagccagc tctgaccgg gactactgt gactgttca 
aatgcattt aactcattt cacctttca cgattctct 
cataattgt cttgacata ttcgtaggt taagtgcag 
gcggccacg atgtacaga gtatcgacc acatcgccg 
aggtacagc taacctatc tgaagatgt cgtctccgt 
caaacgaac ccctgctct tactgtcgt gcctttgag 
cgcggccat tgccagcat tattaaagt tcgagggct 
gtcgttatg caacgagcg tatgcgagt gcataccta 
gacacccga gaaatttaa gcgttttcg catgagtgt 
tattcaatt tttttgatg gaatatttt aacttaaaa 
cacgttgct atggggagc gtgttttaa aatagtgaa 
aacacgttg ctatgggaa acgtgtttt aaaatagtg 
tataatcta ggaaaaaac attattgca tcgtatatt 
tgaaaattc tttaggaac atcctcggt taaatttga 
cgagtcttg caatcacag tttgtaaat ttaattata 
attgttata gtaattttt ttatacaaa ttttcattt 
gcagcggtt catttaaaa tatcacagt taatgaacc 
cttcacaat aactgaata attctttct gcacttgat 
agccaaagg ttcgaaggg tcttttcaa aaagtaaac 
tttggaaaa atacgataa aattgtaat tagtggatt 
cgcacattg tttcagagg atacactgt tgatggcaa 
aaaaacgaa cactgtaac gaaaaaata taaaaatta 
cactgaaat ctctcttaa aggacacct cctatgagc 
ggacgcctc tgataaacg gacattttt gttggctct 
gttttgagc taatgtatt ctaaatagt attgtttct 
cttttaagc ggactctcc aataaactg agaatcaca 
taagccggt acatgtttc atttcagtg ttaaactaa 
gtcagattc agacctcag aagataaaa ttatcttat 
gggtattat ttattgcac tagttcttc tatgcagcg 
ttttaatgg catataaaa ctaaaaaag cgtatgcta 
acactaaaa gaaaaaaaa aacaaaatt ttagcaatg 
aaatttgaa atacctaag cagcaaata ttgtcaaaa 
atgaagatg aactcgtgg tagaaacgt ttgtgaaaa 
gcaaagttt tttttaaaa attaaagga ctgaaaata 
ttgataaat taacttatg gttggttta tcaaatcat 
taaaaaata ttccacttt cttgatcaa ttgttggga 
tgaaaacta aagagttgg cgacgaaat tggaaaatc 
aaattttta aggcaccat taaggtgac aagaaaagt 
tttaaaatc acgatacaa gttttaagt acatatctg 
atgaacatg atatagaga agtcagaag ttaaatatt 
ttttttatg aaatagttt ataacaatt gttgtacca 
atacttata atcatgctg ctacctatt taataaatt 
ctttttagt ttg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY